Screening_Superlative_Packaged_Fruit_Juices_Nutritional_Values.pdf

SSR Inst. Int. J. Life Sci.
Rai, 2019
DOI:10.21276/SSR-IIJLS.2019.5.1.11
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 01 | Page 2184
Screening of the Superlative Packaged Fruit Juices for Nutritional
Values
Sunil Kumar Rai*
Student, Department of Biotechnology, Rapture Biotech, Noida, India
*Address for Correspondence: Mr. Sunil Kumar Rai, Student, Department of Biotechnology, Rapture Biotech, Noida
Sector-10, India
E-mail: sunilraiself2015@gmail.com
Received: 10 June 2018/ Revised: 24 Oct 2018/ Accepted: 21 Dec 2018
ABSTRACT
Background: The fruits are an excellent source of balanced diet because it provides essential nutrients. The packaged juices
claimed the high level of nutrition values, therefore the study focuses on the parameters of purity and health nutrition has been
concluded by screening the most popular fruit juices in India on various parameters.
Methods: The study deals with the analysis of best-packaged fruit juices by identifying standard pH value using pH meter,
microbial contamination ration on NAM, phytochemical properties, quantitative and adulteration analysis, significant amount of
vitamins and minerals, and antimicrobial activity.
Results: The objective of this study was to assess the microbial quality of the fruit juices of different companies. The natural juices
were beneficial for health on the basis of pH value. Tropicana pomegranate had the highest value in microbial contamination
analysis (2.3x103
cfu/ml). Tannin and flavonoid showed resist against cancer and diabetes diseases. Tropicana apple juice with
protein lowest concentration (60.33 µg/ml) was beneficial. Real pomegranate with maximum phenol concentration (9.06 µg
GAE/ml) was beneficial against some diseases. Natural pomegranate with maximum carbohydrate concentration (7.69 µg/ml) was
beneficial. Natural apple with the lowest tannin concentration (-0.38 µg TAE/ml) was preferred. Real apple has the largest zone of
inhibition (20 mm) shown maximum antimicrobial activity.
Conclusion: The data obtained from the study concluded that Real fruit juice somehow is good for consumption, conversely not
suitable for long term and daily consumption because packaged juices become carcinogenic. The observations are probably not
stable and get over-involved on long time storage.
Key-words: Adulteration, Antimicrobial activity, Packaged fruits juices, Phytochemical analysis, Nutrition values, Quantitative
analysis, Secondary metabolites
INTRODUCTION
Nutrients like carbohydrates, proteins, fats, vitamins etc
plays vital and specific role for the development of body.
The study contract with verdict out different
constituents and compositions of fruit juices and
analyzing the category of nutrient available in foods
helps to plan a balanced diet [1]
. The juices of fruits are
considered as highly preferred beverage universally to
humans. Generally, it get conserved by applying
chemicals as preservatives and as well as through the
How to cite this article
Rai SK. Screening of the Superlative Packaged Fruit Juices
for Nutritional Values. SSR Inst. Int. J. Life Sci., 2019; 5(1): 2184-
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natural factors while production.
Somehow, the various microorganisms present in fruit
juice generate "food-borne diseases" and may also cause
death of patient. Fruit juices are very popular among the
people of all ages around the world. Ready to drink
packed fruit juice is becoming more and more popular.
Advantage of packed fruit juices are that these are
convenient to carry and can be kept for a considerable
amount of time. Fruit juices by their very nature contain
various organisms [2,3]
. Clearly, many of these
microorganisms will be harmless yeasts and saprophytic
bacteria. By detecting this bacterial load in the juice, it
apparently gives an about the quality of the sample [4]
.
Basically Juices are processed by mechanic squeezing
and blending of fresh fruits and vegetable flesh at room
temperature without exposing to high temperature or
solvents along with valuable nutrients comprises of
Research Article

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vitamins and minerals to supply a vigorous diet [1,4]
. A lot
of marketable juices are refined to eliminate fiber or
pulp although lofty pulp fresh apple juice is most
accepted beverage. The fruit juices are generally
distinguish into wide variety on the bases of their
extraction and storage process. The concentrated fruit
juices are highly well-liked means to extracted juices
from the fruits moreover next the water content gets
reduced (by evaporating process) [5,6]
. The huge amounts
of concentrated juices, generally frozen state are
shipped to the other country for packaging and
marketing. The fruit juice packaging companies
reconstitute the juice by adding water before packaging.
Another popular way to extract juice from the fruit in
country of origin and then lightly pasteurized and frozen,
chilled or aseptically transported to the country where it
will be packed. Freshly squeezed juice extracted from the
fruits and used immediately [7,8]
.
The information provided through the study deals with
testing different fruit juices samples to identify the
components and chemicals presence. The studies were
allowed to analysis the packaged juices at different
parameters of health benefits, purity and hygiene. The
study places an interest towards phytochemical analysis,
quantitative analysis and adulteration. The study also
highlights the antibacterial properties, antifungal
properties as well as the microbial contamination ratio in
the juices.
MATERIALS AND METHODS
Total six samples of fruit juices collected from the local
market of Noida, India in the duration of 2017. Fruit
juices samples required to perform the necessary tests
were mentioned below, which included some collection
of Real and Tropicana juice and natural fresh market
juice of required flavor Real apple, Tropicana apple,
Natural apple, Real pomegranate, Tropicana
pomegranate, and Natural pomegranate.
Determination of fruit juice’s pH - The pH of healthy
juice should be more than pH 3.5. For pH testing, pH
meter was used.
Detection of microbial contamination- In six samples of
fruit juices, two varieties of fruit juices (Apple, Tropicana)
were chosen based on the consumer demand. Juice
samples were tested within an hour after purchased.
About 0.1 ml of all samples were uniformly spread onto
the NAM and incubated for 24 hours at 37o
C [9,10]
. The
plates were allow for screening to identify the presence
of distinct colonies following incubation period along
with the definite numbers of bacteria, were estimated as
colony forming unit per ml (cfu/ml)[11-15]
.
Phytochemical analysis- For testing presence of fats
takes clean test tube and added 1 ml of each fruit juice
samples. Using a pipette, add the 2 ml of ethanol to each
test tube. Stirrer with glass rod and added 2 ml of
distilled water in tube and stirrer again [11,17,18]
.
Fat is present, if the solution turns in white color. For
analyzing the cardiac glycoside, 1 ml of all extract was
treated glacial acetic acid of about 0.5 ml that include
one drop of solution of ferric chloride solution and
underplayed with 0.2 ml concentrated sulphuric acid. At
the interface a brown ring formed and violet ring might
be emerging beneath the ring, whereas a greenish ring
might be formed in the acetic acid layer.
For phenols, 2 ml of distilled water was further added to
1 ml of extract behind few drops of 10% aqueous ferric
chloride.
For flavonoid analysis, dilute sodium hydroxide was
added to 1 ml of extract. An intense yellow color was
produced in the plant extract, which becomes colorless
on addition of a few drops of dilute nitric acid indicated
the presence of flavonoide [16]
. For tannin test, added 2
ml of 5% ferric chloride to 2 ml of sample [9-11,19]
.
For test saponins, added 2 ml of sample to clean tube,
using a pipette added 4-5 ml of distilled water and noted
for the stable froth formation indicated presence of
saponins.
For analyzing protein, same amount of “5% solution of
sodium hydroxide” and “1% copper sulphate was
added”. Here the pink or purple color indicated the
occurrence of free amino acids and proteins.
For testing presence of benedict’s, 1 ml of a sample
solution was placed in a test tube. Two ml of Benedict’s
reagent (A solution of 3.46 g sodium citrate, 2 g sodium
carbonate and 0.346 g copper sulphate mixed with 20 ml
distilled water) was added [20,21]
. Place test tube on water
bath for 5 min and absorbed the color change and
formation of precipitate.
For fehling test, the 1 ml of Fehling A, 7% copper
sulphate (0.7 g CuSO4 in 10 ml distilled water) and
Fehling B, 25% sodium hydroxide, and 35% potassium
titrate (2.5 g NaOH and 3.5 g potassium titrate in 10 ml
distilled water) solution were mixed and boiled for one

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minute. Similar amount of extract were added and allow
to heat onto the boiling water bath for 5–10 min.
Appearance of the yellow colored first and then brick red
colored precipitate revealed the presence of reducing
agent. For mollsch’s test, added 2 ml of carbohydrate
solution in a test tube and added then 2 drops of
α-naphthol solution [11]
. Suspiciously incline the tubes
and pour concentration H2SO4 using a dropper closer to
the wall of test tubes.
Quantitative analysis
Estimation of protein by Lowry method- We prepared
the standard BSA solutions from 0 to 1 mg/ml
concentration in boiling test-tubes by suitably dilution of
BSA stock solution and kept the volume of solution 5 ml.
Added to the 2 ml of Lowry reagent having 5 ml of
unknown sample test tube then added 0.2 ml of “Folins-
ciocalteau reagent” and determined the absorbance at
660 nm using spectrophotometer [18,19]
. Plot the graph
with protein concentration as well as x-axis and
absorbance at y-axis and prepared the standard
calibration graph and calculated the value of proteins in
unknown samples by using slope value of the graph.
Estimation of reducing sugars by the di-nitro salicylic
acid (DNS) method- Taken 7 clean dry test tubes and
pipette out “standard sugar solution” ranges from 0 to 3
ml in different test tubes and constituted the volume to
3 ml with distilled water concentrations ranging from 0
to 750 mg and then added 1 ml DNS reagent and closed
the test tube with cotton plug after that kept the test
tube on boiling water bath for 5 minutes later then kept
the test tubes at room temperature [22]
. Examined the
extinction at 540 mm aligned with the blank. Prepared
the standard curves of the sugars provided and used
them to estimate the concentration of the unknowns
provided.
Estimation of tannic acid- Added 0, 100, 200, 300 and
400 mg/ml of tannic acid working solution from standard
in 4 test tubes and make up the volume to 5 ml using
distilled water. About 2 ml of reagent 1 was added to
each test tube for analyzing absorbance within 10
minutes. Absorbance was observed at 605 nm for each
sample by spectrophotometer [22,24]
. We plotted a graph
with protein concentration on x-axis and absorbance
on y-axis and prepared a standard calibration graph and
then calculated the amount of tannic acid in unknown
sample by using slope value of the graph.
Estimation of phenol- Standard gallic acid (0, 1, 10, 20,
50 μg/ml) was placed into clean test tubes. Added
distilled water to make up the volume up to 500 µL.
Added 2.5 mL of “Folin-Ciocalteu’s” reagent to each test
tube and incubate at room temperature for 5 min [18]
.
And then added 2 ml of 75% Na2CO3 to each test tube
and incubated at room temperature for 1.5 hours [15]
.
Measured the absorbance of each sample at 765 nm
wavelength using the spectrophotometer and
maintaining protein conc. on x-axis and absorbance on y-
axis to plot standard calibration graph and by using slope
value of the graph calculate the amount of phenols in
unknown samples.
Adulterate analysis of fruit juices for sodium benzoate
and formalin- Taken the 5 ml of sample into a beaker
along with 500 ul of 10% NaOH solution and 6 g NaCl.
Added sufficient water to bring the volume up to about
50 ml and let it stand for 30 min with frequent shaking
[19]
. Added one drop of phenolphthalein (ph.ph) (the
color will change) and drops of HCl until the color change
(disappear) then added excess 3 ml of HCl and 6.25 ml of
chloroform and transferred into separator funnel. Let it
stand for 20 min with frequent shaking [17]
. Transferred
12.5 ml of the chloroform layer (lower layer) into a
conical flask and then added 12.5 ml of 50% ethanol
solution followed by titrating with 0.05M NaOH and
added to the 1 drop of ph.ph as an indicator. For the
analyzing presence of formalin, taken 1 ml of sample in
test tube and then about 500 µl of concentrated
sulphuric acid was added to the side walls of the test
tube without shaking [25,26]
.
Determination of Vitamin C by iodine titration-
Prepared to the solution of 1% starch solution and added
0.50 g soluble starch to 50 ml boiling distilled water. For
titrating with juice samples, added 25 ml of juice sample
to a beaker and then added about 1 ml starch solution.
Titrate until endpoint reached [20]
. Recorded to the initial
and final volume of iodine solution, required producing
the color changed at the end point and calculated the
amount of titrate used for each flask [27]
.

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Antimicrobial activity of different fruit juices by disc
diffusion method- NAM for bacteria and PDA for fungus
was prepared according to the accurate composition.
Immediately after autoclaving, allowed it to cool at
room temperature. Freshly prepared medium was pour
into petri-plates and allowed to cool to room temperature
and stored at 4C. About 100 µL of bacteria culture and
150 µL of fungus from freshly prepared culture was taken
in the pipette and poured in the middle of the respective
petri-plate [26,27]
. Swab the bacteria and fungus evenly on
the surface of the plate. Sterilized the forceps using a
flame and dip in the samples and antibiotics for 5–10
seconds and placed to each disc gently on the agar plate.
Let the plate rest in laminar airflow for 30 minutes so
that the drug was properly absorbed in the gel. Inverted
plates and incubated plates overnight at 30°C.
Calculation
y= mx+c
x=(y-c)/m
y= y-axis, x= x-axis
RESULTS
The research work had been conducted at the
Department of Biotechnology, Rapture Biotech, Noida,
India. In this study, most popular packaged juice samples
were focused collected. Two varieties of fruit juices
(Apple and Tropicana) were chosen based on the
consumer demand. Samples were tested within an hour
after procurement.
Fruit juice having low pH tends to acidic nature. As we
had determined the pH values of different fruit juices
(Apple and Tropicana) from different spices like natural
with tropicana and real brands. It is shown in Table 1,
natural apple juice had pH value 3.8, was lowest in
comparison to normal range is between (6 and 7.5).
Optimally, we want a pH of 7.365. As same as natural
pomegranate juice had pH value 3.8 and according to pH
concentration the healthiest pH is one that is slightly
alkaline. Natural (Apple and Tropicana) was slightly
approaching alkaline nature or less acidic. But tropicana
apple had pH value 3.5 (which is near the pH of natural
juices) (Table 1). Microbial contamination of juices had
shown the hygienic nature of the fruit juice. The juice
contains the maximum value of contamination may be
beneficial. Tropicana pomegranate has the highest value
(2.3 x 103
cfu/ml) so it will may be beneficial in among all
above fruit juices (Table 1).
Microbial contamination of different fruit juice
Table 1: Microbial contamination value of different fruit juice
S. No. Different fruit juice pH value No. of colonies cfu/ml
1. Tropicana apple 3.5 15 1.5 x 103
cfu/ml
2. Tropicana pomegranate 2.9 23 2.3 x 103
cfu/ml
3. Real apple 3.1 15 1.5 x 103
cfu/ml
4. Real pomegranate 3.3 9 0.9 x 103
cfu/ml
5. Natural apple 3.8 – –
6. Natural pomegranate 3.9 – –
Phytochemical analysis- Phytochemical test was
performed on all sample juices, which included fat test,
cardiac glycoside test, phenol test, flavonoid test, tannin
test, saponin test, protein test, bendicts test, Fehling test
and mollish’s test (Table 2). In case of apple juices,
saponin was highly present in natural apple juice and in
the case of pomegranate tannin and flavonoid were
highly present in all three (Real, Tropicana and natural).

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Tannin and flavonoid resist against the disease cancer
and diabetes, these are very beneficial for health.
Protein estimation test was performed on all samples to
find out the exact amount of protein presented in the
juice sample. The highest concentration of protein
present in the real pomegranate juice (332.6 µg/ml) was
beneficial among all other juices. Tropicana apple juice
with protein concentration (60.33 µg/ml) was the lowest
concentration.
Table 2: Qualitative phytochemical analysis of apple juice and Pomegranate juice
S No. Phytochemical
Test
Pomegranate
natural
Natural
apple
Pomegranate
real
Real
apple
Pomegranate
tropicana
Tropicana
apple
1.
Fat +/- +/- – – + –
2.
Cardiac
glycoside
– – – – + +
3.
Phenol ++ + ++ – ++ –
4.
Flavonoid +++ ++ ++ +/- +++ ++
5.
Tannin +++ ++ +++ + +++ –
6.
Saponnin + +++ ++ - ++ +
7.
Protein ++ ++ + + + +
8.
Benedict’s Dark/Brown
ppt.
Brown Brick Red Red Green/Yellow
ppt.
Orange/Red
ppt.
9.
Fehling ++ +++ ++ + + ++
10.
Mollisch’s + ++ +++ + ++ +
(+= Slight present, ++= Moderate present, +++= Highly present, -= Absent, +/-= Maybe present)
The highest concentration of protein present in the real
pomegranate juice (332.6 µg/ml) was beneficial among
all other juices. Tropicana apple juice with protein
concentration (60.33) was lowest concentration (Fig. 1).
Not everyone needs the same amount of protein.
Protein was modest 0.8 grams of proteins per kilogram
of body weight.
Carbohydrates were present in about every living
thing and co-operate a significant role in
the appropriate performance of the immune system,
fertilization, blood clotting, and individual’s growth.
Carbohydrates provide energy to body, with minerals
and vitamins. Simple carbohydrate reduction involves
reducing amount of carbohydrate consumed to between
5 to 45% of daily calories. Natural pomegranate with
maximum concentration (7.69 µg/ml) of carbohydrate
was the beneficial for health but real pomegranate (3.47
µg/ml) has more beneficial in comparison to Tropicana
(Fig. 2).

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Fig. 1: Quantitative estimation of Protein from different fruit juices
Fig. 2: Quantitative estimation of Carbohydrate from different fruit juices
The use of concentrated phenol for the skin may root the
severe damage of skin. Phenol had huge benefits such as
preservation, decreasing the risk of cardiovascular
diseases, cancer, diabetes, Alzheimer’s disease and
arthritis. Real pomegranate with maximum
concentration (12.03 µg GAE/ml) of phenol was more
beneficial with compare to Tropicana (Fig. 3). The high
amount of polyphenol intake with 30% reduced the
mortality (rate of death) in older adults.
Tannins was commonly referred to as tannic acid and
had been accounted to be conscientious for reduction in
food intake, growth rate, food efficiency, total
metabolizable energy and protein digestion.
Consequently, the foods that are rich in tannins were
measured to be of low nutrition. Additionally tannin too
reported to exert other physiological property as it
increase blood clotting, decrease blood pressure, fall in
serum lipid level, develop liver necrosis, and adjust
immune-responses. The amount and variety of tannins
was dangerous to these belongings. Natural apple juice
had lowest concentration (0.38 µg TAE/ml) was
preferred fruit juice but real apple juice with
concentration (32.5 µg TAE/ml) was better than
Tropicana (Fig. 4).

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Fig. 3: Quantitative estimation of Gallic acid from different fruit juices
Fig. 4: Quantitative estimation of tannin from different fruit juices
Analysis of sodium benzoate and formalin- Sodium
benzoate was used to preserve the fruit juice for the long
period of time; it might be the cause to asthma and
cancer disease. If sodium benzoate is present in high
amount then there will be a negative effect on health.
About 1,500 mg of sodium is daily requirement of old
age and high blood pressure patient and less than 2,300
milligrams of sodium in a day for rest of them. Both
natural apple and pomegranate consist of slightly 0.21%
of sodium benzoate. As same as in real apple
the presence of sodium benzoate was very less 0.21%,
so real apple juice was more beneficial compared to the
Tropicana juice. Formalin was a preservative and can
preserve juice for long time. Due to high toxicity, it was
considered to cause liver and kidney. As per the
experiment formalin was absent from all fruit juices so
formalin had the negative effect on the health, it is good
to avoid. Ascorbic acid titration was performed to find
out the amount of Vitamin C in all sample juices (Table
3).

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Table 3: Consist of data obtained during the Ascorbic acid titration
S. No. Different juice Initial titration (ml) Final titration (ml) Difference (ml)
1. Tropicana apple 24.4 46.3 21.6
2. Real apple 43.5 48.3 4.8
3. Natural apple 44 49.5 5.5
4. Tropicana pomegranate 46 49.8 3.8
5. Real pomegranate 45.9 46.1 0.2
6. Natural pomegranate 46 47 1
The real pomegranate had higher zone of inhibition for
bacteria 1 (B. Subtilis) (4 mm) and bacteria 2 (S. Flexneri)
(10 mm) (Fig. 5). Real apple had the largest zone of
inhibition (20 mm) (Fig. 6), the most beneficial juice in
among all above.

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Fig. 5: Disk diffusion result of different fruit juices again four isolated Bacteria
Bacteria 1- Bacillus subtilis, Bacteria 2- Shigella flexneri
Bacteria 3- E. coli, Bacteria 4- Enterobacter cloacae
Fig. 6: Disk diffusion result of different fruit juices again four isolated Fungi

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DISCUSSION
There were six fruit juice samples collected: Natural
apple, Natural pomegranate juice, Real apple, Real
pomegranate juice, Tropicana apple, and Tropicana
pomegranate juice to perform the analysis. Initially the
pH of all sample juices were checked, by taking some
sample juice in bicker and poured the pH meter in this,
the result was in both natural juices apple (pH 3.8) and
pomegranate (pH 3.8) pH value have high (means less
acidic in nature) so the natural juices were beneficial for
health on the basis of pH test compare to other juices
[26]
. Sometimes the juices were packaged in hygienic
manner to increase the sustainability of juices for long
period but according to health issues, it has a negative
effect on health so to find out the quantity of
compounds we performed the microbial contamination
on the packaged juices real and tropicana. Tropicana
pomegranate has the highest value in microbial
contamination analysis (2.3x103
cfu/ml).
The phytochemical test was performed on all sample
juices, which included fat test, cardiac glycoside test,
phenol test, flavonoid test, tannin test, saponin test,
protein test, bendicts test, Fehling test and mollish’s test
[19]
. In case of apple juices saponin was highly present in
natural apple juice and in the case of pomegranate
tannin and flavonoid were highly present in all three
(Real, Tropicana and natural) [8]
. Tannin and flavonoid
resist against the disease cancer and diabetes, these are
very beneficial for health. Protein estimation test was
performed on all samples to find out the exact amount
of protein present in the juice sample. The highest
concentration of protein present in the real
pomegranate juice (332.6 µg/ml) was the beneficial
among all other juices. Tropicana apple juice with
protein concentration (60.33 µg/ml) was the lowest
concentration.
Quantitative analysis for phenol was performed to find
the exact concentration of phenol present in the sample.
Phenol had a number of negative submission like skin
damage even though phenol had even more beneficial
effects such as preventing and/or decreasing the risk of
cardiovascular diseases, cancer, diabetes, alzheimer’s
disease, and arthritis [27]
. Real pomegranate with
maximum concentration (9.06 µg GAE/ml) of phenol can
be beneficial against some disease. Quantitative analysis
of carbohydrate was performed on all samples to find
out the concentration of carbohydrate in sample juices.
Carbohydrate is the main nutrient for health. It played a
vital function in the appropriate performance of the
immune system, fertilization, blood clotting, and human
development [24]
. Natural pomegranate with maximum
concentration (7.69 µg/ml) of carbohydrate is beneficial
for health rather than any others. The quantitative
estimation of tannin was carrying out with each and
every juice sample to locate out except concentration of
tannin. Occasionally, tannin has been accounted to be
liable for diminishing in feed ingestion, growth rate, feed
efficiency, total metabolized energy and protein
digestion ability [9,17]
. Consequently, the foods with high
amount of tannins were measured to be of low
nutritional values. Similarly pr equivalently tannin
furthermore reported to apply additional physiological
possessions, such as blood clotting acceleration, blood
pressure reduction, fall in serum lipid level, produce liver
necrosis and alter immune-responses [23]
. The quantity
and variety of tannins are serious to these properties.
The Natural apple juice with the lowest concentration
(-0.38 µg TAE/ml) was the preferred fruit juice.
Adulterant analysis of sodium benzoate and formalin was
performed on samples to find out the percentage of
sodium benzoate [11]
. Tropicana apple consist of 2.55% of
sodium Benzoate. If Sodium Benzoate will present in high
amount then there will be a negative effect on health.
Formalin was additionally a preservative and can protect
juices for long duration. Due to high toxicity, it was
considered to cause liver and kidney [22]
. As per the
experiment formalin was absent from all fruit juices. So
formalin had the negative effect on the health, it is good
to avoid.
Ascorbic acid titration was performed to find out the
amount of “Vitamin C” in all sample juices. “Vitamin C” is
the master vitamin for our health [9]
. The amount of
vitamin C should be high in daily diet for proper growth
and functionality of body. Antimicrobial activity was
performed on all samples against different bacteria and
fungi. Zone of inhibition represented the presence of
antibacterial. Higher zone of inhibition is beneficial
against the bacteria and fungi. Some disease like typhoid,
TB etc is mainly caused by Bacillus bacteria and some
other bacterial sp. Real pomegranate has higher zone of
inhibition for bacteria 1 (B. Subtilis) was 24 mm and
bacteria 2 (S. Flexneri) was 10 mm so it is most beneficial
for these types of diseases. Real apple has the largest
zone of inhibition (20 mm), which represents the

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maximum presence of antibacterial against the fungi,
also the most beneficial juice in among all above.
CONCLUSIONS
Human body requests significant nutrients to uphold the
vigorous condition. The food must include good quality
of nutrients. This study sentenced out a variety of
ingredients and composition of fruit juice. The data
illustrate that natural juices are beneficial for health on
the basis of pH test compare. Tropicana pomegranate
juice has highest microbial contamination value. Tannin
and flavonoid resist against cancer and diabetes. The
highest concentration of protein present in the real
pomegranate juice. Real pomegranate juice with
maximum concentration of phenol fights against few
diseases. Natural pomegranate with maximum
concentration of carbohydrate is valuable. Natural apple
with the lowest concentration of tannins is most
appropriate. Formalin has the negative effect on the
health and good to avoid. Real pomegranate has higher
zone of inhibition for B. Subtilis and S. Flexneri, so it was
most beneficial for diseases caused by the bacteria. Real
apple juices have the largest zone of inhibition.
In recent years packaged fruit juices becoming the first
choice of consumers and demand increasing more day by
day. However, the packaged juice has some limitations
regarding balanced nutrition. Nowadays consumers have
an astonishing preference towards health and take the
option. However, if the nutrition level of jutes were
protected by using some natural ingredient may improve
the consumption by consumers. The packaged juice
sector may introduce improvement for consumer and
product.
ACKNOWLEDGMENTS
I would like to offer my sincere thanks to Dr. Faiyaz
Ahmed, department of Biotechnology of Bhimrao
Ambhedkar University, Muzafferpur, India. I am also
indebted thanks to Dr. Vinod Kumar Gupta, Rapture
Biotech, Noida for their precious support in carrying out
this work.
CONTRIBUTION OF AUTHORS
All authors equally contributed in this article.
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