Ocearch And Its Effects On Sharks Essay

Ocearch And Its Effects On Sharks Essay
The shark population has dropped 80%. More than 10 million sharks die every year. Ocearch has
been tagging sharks for a long time. People think sharks are vicious beast that mean to eat and kill
humans but they have benefits that the whole world uses everyday!
To begin with, great white sharks are mythical beasts and are fascinating.To start with, the
ecosystem that sharks live in is the Pacific ocean or seas all around the world. When sharks attack
they don't want to eat humans but might mistake us for wounded seals which is their main food
source. Next, after sharks give birth they leave their young. However, once they do leave they could
get killed by other sharks or killer whales. But once they grow up, they can grow to be about 24
feet.
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The Identification of Green Fluorescence Protein Tagged...
Introduction:
Information is passed between neurons and muscles through the release of neurotransmitters. These
neurotransmitters are stored in synaptic vesicles in synapses. But before they can dock at a synapse,
a motor transport known as kinesin moves the synaptic vesicles along microtubules to their final
destination in the cell. This process is known as vesicle transport.
The purpose of these experiments were to gain a better understanding of the movement of vesicles
along microtubules and the importance of this in proper nerve transmission. This was accomplished
by analyzing the phenotypes of three different strains of Caenorhabditis elegans using microscopy
and a fluorescence microscope. The organisms contained fluorescence tags on the membranes of
their synaptic vesicles to make it easier to see how the mutations affected the vesicles locations.
Wild type C. elegans should be actively moving in an s–shape pattern under a microscope. Under a
fluorescence microscope, the tags should appear at the nerve ring and along the nerve cords. In the
C. elegans with a fusion defect, its movement would appear impaired and the fluorescence tags
would be more concentrated at the nerve ring. Lastly, a C. elegans with a transport defect would also
have impaired movement, but the fluorescence tag would be dispersed in patches along the length of
the entire worm.
Results:
Observation of phenotypic appearance was taken using a dissecting microscope at 50x
magnification and a

Personal Narrative: Omnivore
Have you ever played a game in the woods? Or been on a hike at night, with no light? I woke up on
my last day of Atwood. I was a little sad to be leaving, but I was happy to go back home. After I ate
my breakfast, I went to the Glass Class, where we learn about nature and animals. When I got there
the instructors started to talk about food chains and how some animals eat other animals. Instantly I
knew that we would be playing Predator and Prey. Then the instructors said that we would be
playing Predator and Prey! The instructors made us close our eyes so we could get our headbands.
When I opened my eyes I was holding a yellow headband which meant I was an omnivore. Predator
and Prey was like tag. We would go into the woods,

Oz Tag Training : Improving Training
Oz–tag Training: Improving Training In any chosen sport, in order for the performance capacities to
be improved, and to improve one's ability and success within the sport, sport related training must
be implemented. The information on this webpage has been compiled to provide Oz–Tag enthusiasts
ideas and examples of quality Oz–Tag specific training. As stated before in order to be a successful
sportsman, and for performance capacities to improve, sport related training must be implemented.
Rep As well as sport related training, drills must ensure that sport related fitness is increased to
improve the player.  Drills are to be executed at greater intensities than the average game to
improve fitness, the Vo2 Max and the anaerobic threshold.  APP This is because the greater the
VO2 max and anaerobic threshold, the player will have better endurance as fatigue will be delayed.
 Also this training is specific to enhancing fitness to a high level, as sport related fitness is
required to be significantly higher than health related fitness. Two drills that have been implemented
analysed and tested are a 'Tagging Drill' and a drill called 'Keeping Off'. Tagging Drill Many
performance capacities are essential to the game of Oz–Tag in order to be a successful player.
However, the performance capacities of, reaction time, agility and balance have all been targeted by
this drill.  EVA The drill consists of a player running through a course of other players avoiding
getting

Argumentative Essay On Blue Sharks
Did you know that only four out of the 400 species of sharks attack humans? Sharks benefit both
humans and other aquatic creatures. This is why sharks need to be protected and tracked. Sharks
aren't really the demonized creatures we all think they are. To begin with, there are only 400 species
of sharks, only four attack and the blue sharks are not one of them. Most importantly blue sharks are
known for their distinct coloring which allows them to blend in with the ocean water. Blue sharks
inhabits deep waters in the world's temperate and tropical oceans. Other blue sharks that prefer
cooler waters migrate long distances, such as from New England to South America. Squid are
important prey for blue sharks, but their diet includes invertebrates, such as cuttlefish and pelagic
octopuses, as well as lobster shrimp, crab, and a lot of bony fishes. Blue sharks have triangular teeth
that eventually allow it to easily catch hold of slippery prey. On the whole they're actually very
interesting and important aquatic creatures. ... Show more content on Helpwriting.net ...
Specifically, Ciona is an aquatic blue shark named to honor Anna Ciona, a seven year shark
enthusiast from Calgary, Canada. Ciona was tagged at Cape Cod, Canada August 28, 2016. His
length was 8 feet 7 inches, weight 256 pounds, and his stage of life is mature. Ciona's last ping off
the coast of East Hampton, Long Island. Therefore experts say that "Long Island is a possible
breeding ground for sharks." The water Ciona is swimming in is 32 degrees fahrenheit at the
Atlantic Ocean. Blue sharks migrate east across the Atlantic Ocean each year. In conclusion Ocearch
has given important knowledge and facts of many tagged

The Importance Of Bullying In High School
Bill Gates once said, "Be nice to nerds. Chances are you'll end up working for one." I wish that I
had heard this quote before I went to high school. I spent the first part of my education needing
some inspiration similar to this. To explain, I was bullied in Elementary School. From Kindergarten
to Fifth Grade, I was teased because I was too smart. There were several times that I would hide on
the back of the bus crying after a full day of insults. There was one boy at my school that was the
worst. His name was Chaska Lacey. Chaska loved making new insults and nicknames every day to
insult my intelligence. And it wasn't always at school either. Once I was jogging through the town of
Waverly in order to stay in shape for basketball season. I was running at a decently consistent pace
before I passed Chaska and his younger brother, Jolon. I kept on running because I didn't want to
talk to him. He started following and shouting abuse at me. He said things such as, "Hey, Booky. I
didn't know that you could do anything except read. Look at those legs. I didn't think that running
was even possible for someone that spent that much time doing homework. Can you teach me to
read, please?" I eventually out ran him, however it wasn't before his words made me feel that people
didn't know who I was. People only saw that I was a pair of glasses stuck in a book. I know that
what he told me was not incredibly rude, but it still hurt me. I wanted for someone to realize that I
was much more

Mako Shark Research Paper
Shark Essay Do you know what a mako shark is? They are curious beings and are closely related to
the great white shark. Tagged by ocearch, Lazarus and other sharks are helping the ocean's
ecosystem. Mako sharks are exquisite aquatic creatures and are very rare to see. To begin with,
Mako sharks are the fastest shark in the world! Speed sometimes differs between shortfin and
longfin makos. The keel a flat part right before the tail will also help them go faster. They can swim
anywhere from 23 miles per hour to 60 miles per hour! Another fact is makos eat food whole. Even
if they have 8 sharp rows of teeth. Makos need a lot of food. Once a 120 pound swordfish was found
inside a 700 pound mako! Then, they can live in deep water to

Distractions In Children Research Paper
Explain to the children that we are living in a world full of distractions. These distractions prevent
us to hear or recognize God's voices clearly. Even the devil will try to prevent us from hearing God's
call. God's call is unexpected; you don't know when he will call you. It can be in the middle of the
night, anywhere and anytime so we must be ready by being alert and awake like Samuel who no
matter how many time the voice called him, even though Samuel does not know it was God, he still
does not ignore the voice. Imagine yourself as Samuel, will you ignore the voice after the first time,
the second time. Remember that Eli does not tell him it is God who call him until after the time. Can
you be like Samuel and constantly be alert to the voice or will you ignore it and continue to sleep?
This game we about to play is call "Here I am" to teach you how to listen to the God's voice among
the ... Show more content on Helpwriting.net ...
Those who paly Samuel and God don't need it and you can hold it for them for the time being. But
this can be done after you select the child to be God.
2) Have the children gather around together in circle or spread around everywhere in the room. Then
selected one child to be Samuel. Blindfold the child and turn him around a few times.
3) Select another children to be God. Take away both of these children's instrument and keep it for
them since they don't need it now.
4) Tell the child who play God to call out "Samuel" to the child who plays Samuel. While doing
this, the other children will use their music instruments to create distraction for Samuel to find God.
5) The goal is for Samuel to find God. He will do this by tagging the child who plays God. When
Samuel tags God, he will says, " Here I am"
6) If he tagged the wrong person, the person will play the role of Eli and says, "I did not call you.
Go back to sleep." Samuel will go back and God will continue to call Him until Samuel response to
the right

Research Paper On Seekers
One day I went outside to play hide and seek with my neighborhood friends. I usually say I can't
because I have a lot of homework, or I have to help my mom something. I am usually not in the
mood to play with them, but when I am, I am happy to. I said yes and they led me to the area where
we play. Once we got there the group leader started to explain the rules. One of the main rules was
not to leave the area to hide, or you are automatically out. Since I have been with them a couple of
times, I didn't really listen. I was focusing all of my attention on a squirrel that had just found a nut.
When he finished we finally went off. There were about ten people, so we had 2 seekers. If you got
tagged, you became a seeker to. We all went until the last one remained, and that one was the new
seeker. ... Show more content on Helpwriting.net ...
All of the seekers rushed around the area, looking for us. One of the seekers thought he heard
whispers in the tree, so he alerted everyone else. Once they discovered that we were not there, all
the seekers got mad at him for wasting time. Finally, when they couldn't find us, one of the seekers
said there was one place that we could be. He pointed at us, and we ducked down. We knew it was
over and there was no escape. They surrounded us, joking that they found a good spot to hide. So
they tagged us, and we just accepted it, because there was no way we could get out. After all that, it
was my turn to be the

The Clock Strikes 12 : 30 A.m
As the clock strikes 12:30 a.m., my phone battery drops below 20 percent. Other iPhone users may
know that this is the critical moment at which your phone asks if you want to go into "battery saving
mode". This is the level at which I try to avoid. Being alarmed by this notification, I grab my phone
charger to plug it in. After plugging in the cord, I was immediately alarmed when nothing happened.
I looked down and realized my phone charger had the "Turtleneck of Death." This term refers to the
phenomenon when a phone charger cord gets bunched up at the end until it resembled the folds of a
turtleneck, eventually leading to the charger not working at all. This was my reality on that cold
winter night. It was past midnight so all stores nearby were closed. This meant I was going to have
to choose between staying home to get my homework done without my phone, or abandoning my
school work to drive 20 minutes away to pick up a phone charger at one in the morning. This was
not a hard decision because I knew I wouldn't make it through the night without a phone. I quickly
changed into my warmest clothes and ran outside to my car. My father probably thought I was up to
no good when he saw me running out the door at 1 a.m., but I was headed straight to Walmart. After
a 20 minute drive I pulled into an almost empty parking lot and ran in to grab a charger. I returned
home at 2 a.m. with a brand new phone charger in hand. I plugged in my phone and everything felt
right again. The

Research Paper On The Chaser
"Wounds turn into scars and scars make you tough," Aisha Tyler. When I was in Pre–K, life was
pretty simple. We would eat snacks, have naptime, and have a long recess. During recess, there was
a game that everyone enjoyed playing. We called it, "Cootie Touch." The game is played like tag.
All the girls are the chasers, and all the boys are the runners. When a boy gets tagged, he becomes a
chaser; and that's how the game is played until there is one person left. The last person to get tagged
wins. There were many times this game got very heated; to the point that we were no longer allowed
to play.
This one day, we go out to recess, and we begin playing our favorite game. We play the first game,
and I get tagged out almost immediately. The second

File Format, Compression Techniques, Image Resolution And...
Impact that file format, compression techniques, image resolution and colour depth have on file size
and image quality – D2
In this report I will be discussing the different file formats, compression techniques, image
resolution and colour depth. I will be explaining the different purposes, then, after I have issued an
in–depth explanation of image quality and file size I will be completing a final conclusion about the
best ones to use for certain tasks.
Seen as there are numerous different uses for images it means that the quality will differ for
different purposes. The formats which I will be discussing within this report include JPEG, GIF's,
TIF, PSD and BMP. All these formats are made for different reasons and I will be explaining these
differences in quality and file size. Each format varies in the depth of the colour within the image,
some have larger colour depth meaning that the file size will be larger, whereas, some of these
formats have very low colour depth resulting in a lower quality image. When exporting an image,
sometimes there is an option to adjust the colour depth to your preference, so, the file format could
change to your desired colour depth.
Compression techniques
When compressing a file to make it smaller there are two options which are available, Lossy and
Lossless. Both do the same job, which is compressing the file size, however, they both do it
differently for different reasons, I will explain the advantages and disadvantages of both. The

Protein Adsorption Using Fluorescently Tagged Rh Fgn
Introduction:
For this laboratory, we are studying protein adsorption using fluorescently tagged Rh–FGN. To
prepare for this study, model sutures of a specific length have been coated in albumin, which allows
us to compare the difference between a coated suture and an uncoated suture in regards to "sticky"
protein adsorption. Based on the effects of surface energy (γ) on protein adsorption, the uncoated
nylon model suture will yield higher protein adsorption then coated suture, since the coated suture
should have higher surface energy. Thus at a 0.05 assurance level (α = 0.05) our null hypothesis is
as follows: there is not a difference in protein adsorption between uncoated and coated nylon model
sutures when exposed to a fibrinogen solution. Our alternative hypothesis (Ha) is as follows: there is
a difference in protein adsorption between uncoated and coated nylon model sutures when exposed
to fibrinogen solution at a 0.05 assurance level (α = 0.05).
Background:
When designing new state–of–the–art biomedical devices, the reaction between the biomaterials
placed in the body and the body itself is highly important and widely studied. The compatibility of
biomaterials governs their effectiveness; therefore, understanding the mechanisms of these
interactions and the thermodynamics behind them is essential for creating and improving these
devices. When determining the materials necessary for these devices, protein adsorption must be
addressed, due to the fact that

Ripped: A Short Story
Ripped
"Yes! It's recess time!" I yelled.
"Awesome," said Hunter.
"Tell everyone to come to the playground," I told Hunter.
"Okay!"
It was my first year of elementary. Every recess, my friends and I would play tag, until our feet
would collapse.
My good friend, Hunter, was the first person to be it every day. We would run far away from Hunter.
He was nowhere in sight. I sat on a rock, wondering what would take him so long. He usually can
tag someone in the first minute of the game. And that was going to be me. I turned around and
Hunter was running full speed at me. My reaction to that was too slow. He tagged me and ran full
speed the other direction, so he doesn't get tagged back. Dang it! I thought. I hated getting tagged.
"Ha–ha! You're it!" yelled Hunter as he got further away from me. I then ran full speed at him.
When I grabbed Hunter's shirt to tag him, I felt that his shirt was very thin. My hand was then pulled
into his direction, until I almost fell. But I managed to balance myself.
"Hunter, you shouldn't even bother to tag me. I'm too good for you," I told him, so he ... Show more
content on Helpwriting.net ...
As I walked inside, there were butterflies in my stomach, trying to get out. "You will get a yellow
card for that, ok? You ripped Hunter's shirt!"
I was left speechless. I have never gotten a yellow card before in my life. This means that my
parents will get notified. I was very mad. The shock of her words left me in place.
That night after school, my parents got an email from Mrs. Shelly. They were shocked to see that I
would do such a thing. They were very disappointed at what I had done. My parents yelled at me
that night until my ear were ripped out of my head.
That day was a very sad day. I first got in trouble at school and then I got yelled at by my parents.
As I lay there in my bed, I could not stop crying. There was nothing I could do to fix it. The best
thing I could do was to fix my behavior. And I

Research Papers for Vietnam War
Vietnam War Research Paper: During the 30 years of XX century Vietnam was at war. It all started
in the 1940′s, when the Communists fought against French colonial rule, and was completed in 1975
with the fall of Saigon. The period, which the Vietnamese know as the "American War" and the
Americans call the "Vietnam War", lasted from 1965 to 1973, during the time of the U.S.
intervention. The communist leader Ho Chi Minh led his forces based in the north and defeated the
French in 1954. Agreements was signed, which divided the country into the pro–American South
and communist north, with a demilitarized zone between them. Continue reading → This entry was
posted in Research papers and tagged how to write a research paper on the ... Show more content on
Helpwriting.net ...
In the study of the spectral range of 400–200 nm devices are used built on the same optical scheme
as those for studying the visible range of the spectrum, the difference is only in the glass prisms,
lenses, and other optical components. When measuring the intensity of UV emission, as a reference
is used source in the UV region of the spectrum with known distribution of the spectral brightness
(band tungsten lamp, coal arc, and synchrotron radiation). A thermocouple and graded solar cells are
standard receivers in this region of the spectrum. Continue reading → This entry was posted in
Research papers and tagged UV Spectroscopy research paper, UV Spectroscopy research proposal
on May 19, 2013.
Research Paper on Tilapia
Tilapia Research Paper: Tilapia is a common name designating fishes of the Cichlidae family. This
brand name comes from the Latin form of thiape, a word that means "fish" in béchouana – an
African language. It includes three genera within cichlids: Oreochromis, Tilapia, and Sarotherodon.
They originate in Africa and Near or Middle East. The size varies between 5 and 50 centimeters.
These fresh or brackish fishes water are bred and consumed widely in the world. The first notes of
the tilapia breeding go back to ancient Egypt 4,000 years ago, but its commercial exploitation
begins in South Africa

Essay On Proteolytic Degradation Tagss
Results and Discussion
Multiple synthetic amino acid sequence tags target fluorescent proteins for degradation by different
proteases. To study proteolytic degradation by different proteases, fluorescent proteins were
engineered with different potential degradation tags on either the N–terminus or C–terminus (Table
S2). Previously tested degradation tags could not be compared directly to one another because they
were characterized in different E. coli strains and under different conditions. To our knowledge, this
is the first systematic investigation of multiple degradation tags in E. coli. We tested the previously
determined degradation tags and several newly designed tags (Table 1A–B). Degradation tags were
fused to multiple fluorescent ... Show more content on Helpwriting.net ...
The fluorescence of RepA70–YFP increased as more RepA70–CFP was produced (Fig. 3C), and
this indicated that ClpAP protease could be overloaded and a proteolytic–queue forms similar to
what was observed with the LAA tagged proteins targeted to ClpXP8. We also tested two other tags,
MarA and MarAn20 (20 amino acids from the N–terminal of MarA), which target proteins to be
degraded by the Lon protease. The Lon protease was weakly overloaded by MarA tagged proteins
but was overloaded more by MarAn20 tagged proteins (Fig. 3C–D). This made us wonder if Lon
could be overloaded when both MarA and MarAn20 were co–produced. Indeed, this was the case
(Fig. 3E).
The main proteases of E. coli can exhibit different levels of crosstalk depending on the degradation
tags used. We have demonstrated that ClpXP, ClpAP, and Lon can be overloaded using two proteins
engineered with identical degradation tags targeted for a specific protease. We have hypothesized
that crosstalk between proteases may occur through shared information (Fig. 1B). To test this
hypothesis in a synthetic system, we monitored the level of a fluorescent protein (YFP) targeted to
one protease while producing another protein (CFP) targeted to a different protease. There was
strong crosstalk when proteins with the LAA degradation tag (target to ClpXP) were co–produced
with proteins with all other tags (RepA70, MarA, MarAn20, and HipB; Fig.

A Research Study On Cultured And Handled At 20. C Using...
Nematodes were cultured and handled at 20 °C using standard methods (Brenner, 1974). C. elegans
strains used in this study are as follows: N2 Bristol and MR156: rrIs01[Pelt–2::GFP; unc–119(+)] X,
which were used as wild type. CB3514: lin–23(e1883)/dpy–10(e128) II, YHS47: lin–
23(e1883)/dpy–10(e128) II; rrIs01[Pelt–2::GFP + unc–119(+)] X, DH2: zyg–11(b2) II. DS97: mat–
2(ax76) II, EU593: mel–26(or184) I, EU640: cul–2(or209) III, UP63: mat–3(ku233) III, NJ582:
cul–1(e1756)/unc–69(e587) III, VC141: zif–1(gk117) III, VC370: rfp–1(ok572)/eT1 III; +/eT1 V,
VC562: rbx–1(ok782) V/nT1[qls51] (IV;V), VC684: rbpl–1(ok907) I/hT2[bli–4(e937)let–?
(q782)gls48] (I;III), VC954: rnf–113(ok1401) III/hT2[bli–4(e937) let–?(q782)qls48] (I;III),
VC1241: skr–1(ok1696) I, VC1439: skr–2(ok1938) I/hT2[bli–4(e937) let–?(q782)qls48] (I;III),
YHS171: rfp–1(ok572) III/hT2[bli–4(e937)let–?(q782)gls48] (I;III); rrIs01[Pelt–2::GFP + unc–
119(+)] X, YHS161: rbx–1(ok782) V/nT1[qls51] (IV;V); rrIs01[Pelt–2::GFP + unc–119(+)] X, and
YHS163: skr–1(ok1696) I; rrIs01[Pelt–2::GFP + unc–119(+)] X.
Quantification of intestinal nuclei and microscopy
To examine the number of intestinal nuclei marked by GFP using an elt–2::GFP transgene, worms
were transferred to 0.2 mM tetramisole in M9 buffer on a poly–L–lysine–coated slide glass, covered
with a coverslip and observed using a fluorescence microscope (Zeiss Axioskop 2, Carl Zeiss,
Germany). Otherwise, DNA of some strains, whose intestine is not marked with intestinal

Questions On Natural Language Processing
Introduction
1.1 Inspiration and Outline
Today in the world of information technology everyone is using internet to share and access data.
But the data is in the form of natural language. And as we all know that all natural languages have
basic feature that it have ambiguity. It is something which can be understand in two or more ways,
and that depends on what situation it occurs. There are different types of ambiguities present in
natural languages like lexical ambiguity, structural or grammatical ambiguity, ambiguity of scope,
pragmatic ambiguity etc. Among all these ambiguities, lexical ambiguity is generally present in
natural languages. This ambiguity can be defined as the ambiguity which occurs because a word has
various meaning. So, to use information technology in best way we need to eliminate ambiguity
from the sentences with the help of tool called word sense disambiguation.
Word Sense Disambiguation (WSD) is a tool which computes the correct sence of ambiguious word
in context which it occur. It is a main challenge in Natural Language Processing(NLP) and it is
considered an AI– complete problem. For example consider the sentence–। and ;g ,d vke ckr gSA
vke Qyks dk jktk gSA Here, the ambiguous word is vke which can be interpreted as 'common' or as
'mango' based on the situation in which it is said.
The problem of recognition of definite sense of given word seems to be simple. As human being
simply identify the meaning of a word given on a given context. But

Argumentative Essay: Marco Polo
Marco Polo was a Venetian merchant believed to have journeyed across Asia.He first left at age 17
with his dad and uncle.They were traveling upon the silk road.Marco Polo was born in 1254 in italy
City–state. He traveled with his family. They journeyed to Europe to Asia 1271 to 1295. There was a
game made after Marco Polo the rules of the game you have to play with five or more people you
have to be in a pool one of the five people will have their eyes close and the four people with there
eye open have to yell "Marco" then the person with their eyes close yell "Polo" the object of the
game is try not to get catched by the person yelling "Polo" and if you get tagged you yell "fish out
of water" and the person who got tagged is the new Polo.

School Observation In School
I observed Mrs. Ruffs First grade class at Carlin Park Elementary. The class consisted of 11 girls
and 11 boys. While I watched the class, I focused in on trying to notice the difference between the
two sexes. I observed that the girls and the boys are similar in the way they carry themselves. They
were both very active in the activity they were playing. The activity they played was a version of tag
that there was one person it and they all needed to run away from the tagger and if they were tag,
they turned into a zombie where they crawled around trying to tag other people that were still in.
The catch of the game is that everyone needed to run on a line in on the gym floor. Boy or girl, they
all were very energetic in not wanting to get tagged. The boys sometimes went off track and didn't
pay attention to directions as much as the girls did but for the most part, the instructions were
explained in detail that the first graders could understand the game and have fun playing it.
(Repeat from first two Observations)
When I asked Mrs. Ruff her opinion about co–ed physical education and at what ages/grades would
it be better to separate the two sexes, her response was passionate which made me feel good and
glad I asked the question. Mrs. Ruff believes that co–ed classes are good for students and do more
good for the kids than harm. She has not had a problem at the elementary level and feels that her
students get more out of being together with one another and that the level of

Hsp 30 Lab Report
In response to heat–shock, yeast extracts up–regulate the HSP 30 gene by producing more proteins
the same way bacteria cells up–regulate gene expression. In an environmental stress, such as heat
shock, proteins inside a cell will degenerate. Once the protein is exposed to heat shock, the weak
interactions that hold the proteins' tertiary structure will degrade. Therefore, the cell must use the
chaperone proteins to re–fold the degraded proteins. In order to create more chaperone proteins, the
HSP 30 gene must be turned on. It is essential that the HSP 30 gene in yeast extracts are frequently
turned on (up–regulated) to produce more chaperone proteins. Therefore, the experiment was
conducted to test the expression of a particular gene, called ... Show more content on
Helpwriting.net ...
The results for the yeast with GFP–tagged HSP 12 are shown in lane 1 (Fig. 1). In figure 1 lane 1,
single bands were obtained at 15 KD and 20 KD, while two–separate clusters of bands were
obtained at 25 KD and 32 KD. In lane 1, a band was found at 32 KD (Fig. 1). The HSP–12 gene
should have produced bands around 38 KD. Faint bands are also present in lane 1 as a result of
background bands or proteolytic cleaving of proteins (Fig. 1). Therefore, the positive control
worked in the experiment. This means the yeast in the experiment were in fact heat shocked. The
results for the wild type yeast strand with no GFP–tagged proteins are shown in lane 4 (figure 1).
The negative control in lane 4 produced no bands (Fig. 1). Since the negative control worked, there
were no contaminations in the lanes that could have potentially skewed the results. The results for
the yeast with GFP–tagged HSP 30 grown at 30°C are shown in lane 2 (Fig. 1). In figure 1 lane 2,
one band was obtained at 22 KD. Band was not produced around 64KD, but was produced at 22KD.
This indicates that when yeast strands are not experiencing heat shock, genes are not up–regulated.
At the Permissive temperature, an appropriate sized protein is not produced because the HSP 30
gene is not exposed to heat shock. The results for the yeast with GFP–tagged HSP 30 grown at 42°C
are shown in lane 3 (Fig. 1). In figure 1 lane 3, single bands were obtained at 21 KD and 22KD.

Things that No One Should Post on Facebook
Nowadays most of the teenager, adult or even kids are using social network which the most
common is Facebook. In case we are not updating our status right now, chances are that we are
uploading photos or taking some sort of abnormal test. We post tons of intimate details about our
lives that we normally wouldn't share with anyone. We think that if we make sure our privacy
settings are set properly that we are safe and snug within our circle of friends. The problematic is
that we never know who is actually looking at our info. Our friend's account might have been
hacked when they installed some rogue submission, or their creepy uncle may be using their account
as they forgot to log out. For the sake of the safety of us and our family, there is some info that we
would never post on Facebook. Here are 5 things that we must reflect eliminating or not posting to
Facebook and/or other social networks. Ourselves or Our Family's Full Birth Dates: We all love
getting "happy birthdays" from our friends on our Facebook wall. It makes us feel all warm inside
knowing that people remembered and cared enough to write us a short note on our special day. The
problematic is when we list our birthday we are provided that individuality thieves with one of the 3
or 4 pieces of personal info that is wanted to steal our identity. It is greatest to not list the date at all,
nonetheless in case we need, however leave out the year. Our real friends must know this
information as well. Our

Fluorescent Tagged Protein Research Paper
Essay 47: Fluorescent Tagged Proteins
Proteins tagged with fluorescent markers are extremely versatile tools in cell and molecular biology
research. A fluorescent marker is, as the name suggests, a molecule that emits light of a particular
wavelength following exposure to a photons of a shorter wavelength, for example a laser beam. If a
fluorescent marker is a reasonable size and chemically stable enough to be attached to a protein, it is
potentially useful to molecular biologists. In many cases, the proteins in question are monoclonal
antibodies generated against a particular target of interest such as a cell surface receptor, a
cytoskeletal protein, or a cellular organelle. In some cases, a fluorescent label is used to track the
fate of a particular protein as it is processed in the ER and Golgi complex, transported along
microtubules, or secreted from the cell altogether. In still other cases, two or more fluorescently
tagged proteins are introduced into the same cell to see whether they localize in the same or separate
compartments or organelles. ... Show more content on Helpwriting.net ...
In this method, cells are incubated with antibodies conjugated with fluorescent dyes such as FITC
(fluoroscein isothiocyanate), which emits green wavelengths of light; PE (phycoerythrin), or Texas
Red, both of which emit red wavelengths of light. Newer fluorescent dyes emit at yellow and blue
wavelengths as

Purified Protein Lab Report
Biotechnology industry, Research, Pharmaceutical industry used purified proteins in large quantities
for their purposes. This able genetic engineer to set up some technics to easily extracted from
variable source proteins in large amount.
Several approaches can be envisaged to address the function of a gene. The techniques of molecular
biology and biochemistry allowing for example to localize the expression of a gene or its product
(Northern, western, in situ hybridization, immunofluorescence, etc.), to determine the structure of
the protein (NMR, crystallography ...) or to find partners proteins (double hybrid,
immunoprecipitation ...) give important information on the function of a gene (V Ecochard –2011).
This experiment will focus on SDS PAGE of purified protein and cell lysate samples, Western blot
analysis procedure, interpretation of purified protein and lysate samples; Sequence analysis of
tagged fusion proteins, and why and how to choose cell type, and why cell expression mater? The
following samples containing human dihydrofolate reductase (DHFR) protein will be analyzed:
Purified His–tagged DHFR, expressed and purified from E.coli, ... Show more content on
Helpwriting.net ...
Place the gel in the electrophoresis chamber and fill chamber with with 1X running buffer, and
carefully rinse wells by pipetting to remove any bubbles.
Sample preparation: purified protein and cell lysate have been already and denatured in Laemmli
sample buffer and are in His–tagged DHFR, GST–tagged DHFR, Myc–Flag–tagged DHFR aliquots.
Control lysate is provided by TA. Wear goggles before to hreat samples for 2 minutes at 95˚C.
Centrifuge samples and load gel at room temperature. Obtain an aliquot of the each of the
following: 1X Laemmli buffer and Kaleidoscope prestained protein standard (Bio–Rad

Personal Narrative : My Brother Nick
One time my brother Nick and I were going to get ready to play a hard, and tiring football game
with our friends. My brother and I were all ready and excited because we were on the same team. I
was getting ready to receive the ball from the other team. We were ready to throw, run, and catch the
ball while moving down my yard. We were both good at football, at least we thought we were. The
other team was also ready for the beginning of the game. When they first kicked off, Nick and I got
a few yards, but not all the way to the "end zone". We got lined up and my brother Yelled "HIKE" so
I ran out and then slanted in and he threw the ball and I caught it. Nick and I yelled "Yes!" because
now we were even closer to where we would get a ... Show more content on Helpwriting.net ...
Nick and I were sad, but it was OK because we were still winning by 7 points. We had to stay
focused even when we were in a bad situation. When they had to kick off we were ready, Nick was
up a little and I was back far. I could tell that they were going to kick it short, so I moved up but let
Nick return this time. so I blocked for him. That was a good choice by both of us because we ran it
all the way back to the end zone. We also kicked it short but we kicked it away from the other team
so they would struggle to get it. It worked, so they got less yards than they usually would. They
were about 30 yards from the end zone. so Nick and I were happy. On the next play they passed the
ball and Nick tipped it away. Next they passed, but made the catch a few yards from the end zone.
So next they ran the ball to try to get the touchdown, and they did. Now the score was 21 to 14 us.
The game was almost over and there were about 7 minutes left. So we were trying to score some last
points to be completely sure we would be in the lead. When we were getting ready to receive the
ball from them, they decided to kick the ball short again. So I yelled to Nick "Leave it" so that we
would not touch it and have the other team get it. Now we had about 5 minutes left and Nick and I
were all ready to start. We formed a bit of a Circle, even though there was only two people. The play
was a slant so that meant that Nick was the

Protein Chromatography Lab Report
During this experiment the His–tagged protein RNase H was purified using affinity
chromatography. The source of over–expressed His–tagged RNase H used was 5.0 mL of E. coli
lysate. The experiment was performed in partners using different materials in order to prepare the
needed three buffers to an approximate pH value of 7.9. Once the three buffers were ready a
minicolumn was packed with 0.5 mL of Ni–NTA agarose and then different solutions were used to
wash the column, starting with DI water, followed by the loading buffer, then the E.coli lysate, the
washing buffer, and finally the eluting buffer. Some of the flows through were collected for future
analysis in the upcoming experiments. The first was after the washing with the E.coli lysate, then
the first wash from the washing buffer, and finally the flow through obtained after the wash with the
eluting buffer. ... Show more content on Helpwriting.net ...
They are key constituents of all biological systems, and perform a great variety of functional and
structural roles. Proteins play a crucial role in almost all biological processes, like signal
transmission, catalysis, and structural support. This important range of functions comes from the
existence of thousands of proteins, which are each folded into a characteristics three–dimensional
structure, the same structure that allows it to interact with one or perhaps more molecules. A lot of
the functional and structural studies of proteins are conducted with purified preparations of proteins.
In general the purification methods of proteins aim to exploit the differences in their different
properties such as, solubility, size, charge, and resin–binding specificity, all with the purpose to
enrich the solution for the targeted protein

The Importance Of Green Fluorescent Protein
In the 1960's, Green Fluorescent Protein was discovered to be responsible for Aequorea victoria's
fluorescence under UV light (Niwa et al., 1996). Today, GFP is often used in protein tagging and has
made it possible for scientists to study expression and track proteins in vivo. With the rising
importance of GFP fusion proteins and other recombinant vectors, the metal affinity of Histidine
helps to make the protein purification process easier (Lilius et al., 1991). His tags are commonly
used to purify proteins through immobilized metal–affinity chromatography (IMAC) (Lilius et al.,
1991). This rapid and efficient method separates the recombinant protein from unwanted products
such as RNA. The His tag DNA sequence is inserted into the ... Show more content on
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It is of strong interest to molecular biologists because of the strong light emission of 508 nm under
UV light. UV wavelengths excite the fluorophore, contained in the Ser – Tyr – Gly sequence near
GFP's N–terminus, and a bright green fluorescence is given off as a result (Niwa et al., 1996). GFP
can be successfully tagged to many proteins without disturbing their function (Feilmeier et al.,
2000). This allows scientists to exploit the protein for its usefulness in marking proteins in vivo.
GFP tagging has many applications. It can be used to study gene expression by inserting gfp after a
promotor in the plasmid, so that if expressed, the colony expressing the gene of interest can be
identified by a green fluorescence of GFP under UV light (Feilmeier et al., 2000). Specific cellular
organelles can be tagged, such as the endoplasmic reticulum, to visualize protein localization and
networking inside the living cell. GFP can also be used to monitor cofactor levels within a cell in
real time. For example, when GFP was tagged to proteins that contain a receptor sensitive to Ca2+,
they interacted in a way so that the fluorescence of the fusion protein was proportional to the
calcium ion concentration with less than a 1 second lag in the cell (Romoser et al., 1997).
Throughout the decades, GFP mutants have been created to give brighter emission and different
wavelengths. Researchers can track separate cell pathways at the same time, since different proteins
can

Examples Of The Greatest Entertainment
America's Greatest Entertainment
America today is a world completely driven by entertainment. Our lives are surrounded by it each
and every day. Entertainment could be anything from TV, movies, music, social media and the
internet to skydiving, mini–golfing and paintball. Since there is so many different forms of
entertainment, and the want for it is so demanding, competitors are always trying to find a way to
one up one another. One classic form of entertainment that seems to be bringing people back to play,
is laser tag. Many different entertainment places offer laser tag but two seem to outshine the rest,
Laser Quest and Main Event. Laser Quest has a futuristic arena that is unmatched. They are in
ownership of the best equipment. ... Show more content on Helpwriting.net ...
Laser quest gives you the ability to select you very own codename. This codename could be
anything from your real name to your favorite superhero. This codename is tied to whichever pack
you select and adds to the fun and entertainment of laser tag. At Main Event, all of the names are
preselected and assigned limiting your imagination and creativity. After a quick game of laser tag at
Main Event you are left with a leaderboard of all the scores of the last mission, you get to see how
you ranked amongst opponents which can either be quite satisfying or, depending on how you did,
disappointing. At Laser Quest, you are offered that same opportunity, but not only that, everyone
receives a personalized scorecard that lets them know how they did. This scorecard shows everyone
that you tagged, everyone that tagged you, where exactly you tagged or were tagged, and how many
lasers you fired. Laser Quest has the best equipment around and blows Main Event out of the water.
Laser Quest creates a sense of competitiveness while maintaining a safe environment. Laser Quest
has its own rewards program. You register your account with your codename and it ranks you across
not only the people at Laser Quest but it ranks you against questors across the world. People are
constantly battling their way to the very top. Laser Quest seems to be all fun and games but they
actually host professional laser

Managing Big Data Systems
Definite answers regarding how much usable data is actually being generated by city–sized
populations, and best places to get it could be the subject of a future study, but social media
platforms may be the source most easily tapped into for this type of data. Employees of local IT
departments are in danger of having difficulties storing and processing data from social media
platforms to use as a foundation for aligning social network analysis with the systems . There is a
growing demand for those having the necessary skillsets to deal with big data. Local governments
(especially smaller–sized ones) might have special difficulty acquiring these resources which makes
finding a comprehensive solution for combining "big data", SNA, and GIS even more difficult, as
data manipulators are in short supply. A survey supporting a Public CIO Special Report ("The
Trouble with Big Data Talent," 2013), finds that more than half of government agencies polled were
experiencing data–related hiring difficulties – meaning there is a shortage of individuals who
possess an engineering background, can model data mathematically and can contribute to
organizations effectively using data insights for decision making.
Using the Data
Using data mining techniques, such as graph mining and social network analysis on regional data
sources could contribute great insights and improve operations. Social networking analysis is the
study and analysis of networks involving social interaction. Types of

Steganography Is The Most Effective And Fastest Media For...
In this modern era, the computer and internet have becomes the most effective and fastest media for
communication that connect different parts of the global world. As a result, people can easily
exchange information and share information with the others via the internet. However, the
information security requires the confidential data that needs to be protected from the unauthorized
users. Steganography is one of the methods used for the secure transmission of confidential
information. It can provide a high level of security to secure the important data during combined
with encryption. Steganography is a Greek origin word that means stegos meaning cover and grafia
to which classified as cover writing. Steganography is hiding a secret message into other
information such as sound, image and video. It is also known as invisible communication. In
addition, steganography is related to two technologies which are watermarking and fingerprinting.
Both these technologies provide the same goals which are mainly used for intellectual property
protection.Thus, both are different in algorithm requirements. Watermarking provides a hidden
copyright protection by owner whereas the fingerprinting used a copy of the carrier object and make
it as unique to the different customer. Besides, image steganography is a collection of numbers that
hide information or message inside the image. In this image hidden method, the pixels of the image
are changed to hide the secret data and invisible to

Cloning of Enhancer of Zeste Homolog 2 in Forward...
Title: Cloning of Enhancer of Zeste Homolog 2 in forward orientation into Escherichia Coli using
histidine–tagged pbluescript II KS+.
Abstract
Enhancer of Zeste Homolog 2 locus is intensely over expressed in breast and prostate cancer and it's
been established that its promoter inhibition by p53 has led to reduced cell proliferation and
invasion (Bracken, 2003; Xiao, 2011). Objective is to clone a forward orientated EZH2 insert into a
his–tagged pbluescript. Cloning EZH2 into a histidine–tagged pbluescript in a forward orientation
potentially allows isolation of protein via Affinity Chromatography or Chromatin
Immunoprecipitation therefore its role, effects and targets in the genome can be established.
Resultant Recombinant plasmids in ... Show more content on Helpwriting.net ...
Its locus is particularly amplified in these noted tumours leading to the progression of these cancers,
it can be suppressed by p53 (tumour/ proliferation suppressor) which represses the EZH2 promoter,
resulting inhibition of cell proliferation and invasion (Bracken, 2003; Xiao, 2011).
The vital components and techniques of gene cloning are as follows, the DNA sequence that
contains the desired gene (EZH2) is amplified by Polymerase chain reaction. PCR was established
by Kary Mullis in 1985, popularly known to amplify target sequences of DNA (EZH2) to a billion
fold in several hours using thermophilic polymerases (Taq) ,primers and other cofactors (Sambrook
and Russell, 2001). Three crucial steps are involved which are Denaturation (at 95°), Annealing of
the forward and reverse primers (55–65°) and lastly primer extension (at 72°). After amplification
the desired sequence is integrated into the circular vector (pbluescript) forming the recombinant
molecule. For the compatibility of the insert and vector, both were digested with (EcoR1) so the
same cohesive ends are generated in both, making it easier to ligate. EcoR1 is a restriction enzyme
that belongs to the type II endonuclease class which cuts within dsDNA at its recognition site
"GAATTC" (Clark 2010; Sambrook and Russell, 2001).
Agarose Gel electrophoresis uses electric fields and ethidium bromide (under UV

Tagged Architecture Essay
 Tagged Architecture, is very flexible memory protection mechanism. Each word in a memory has
an extra bits which dedicated to control access. In implementation of tagged architecture, one
memory word can be designed as a read–only in order to protect data. Another memory word can be
set as read and write or execute–only as exemplified of Figure 5. For example, in some cases some
data values are not changed such as student id, student nationality. Those data can be protected in
read–only memory word. A few systems have a tagged architecture memory protection such as The
IBM System/38, The Burroughs B6500–7500 system, because it is expensive and compatibility of
other hardware.
Figure 5: Tagged Architecture Source: Pfleeger and Pfleeger, 2006
 Segmentation, is protection of based on segment of memory. A program data can be separated
many pieces which are coded different purpose as exemplified on Figure 6. The main advantage of
segmentation is that program cannot identify actually memory address. The operating system has
three advantages of using this method as following:
The operating system can occupy any memory location or relocate.
If segment is empty, it can be removed from memory.
Memory addresses can be examined in order to protect.
According to Pfleeger and Pfleeger (2006), there are four benefit of ... Show more content on
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Radio was one of the first product of company. Nowadays, organization has expanded in electronics
and multimedia industry. First version of the PlayStation was released in 1994 in Japan as a gaming
console. Sony introduced the PlayStation Network and Qriocity online services in 2006. This
service is part of the PlayStation 3 (PS3), and it is free. Each of PS3 user can register and access to
PSN that offers following

Geo Tagged Data
Abstract
The dynamic characteristics of twitter messages have the potential to provide GIS scientists with a
great research opportunity to analyze the diffusion of events such as disease outbreaks,
environmental changes, and social movements. This could be achieved by digitally collecting tweets
that contain geo–tagged data. However, the percentage of geo–tagged data is extremely small
comparing to non geo–tagged data. On the other side, the non geo–tagged tweets often contain
chaotic data noise such as automated robots that affect the accuracy of the tweet content. For this
matter, it is essential to analyze and understand the differences between geo–tagged and non geo–
tagged tweets.
This research intends to compare the context and temporal ... Show more content on Helpwriting.net
...
This framework can enhance the temporal analysis of non geo–tagged tweets in comparison to geo–
tagged tweets for tracking real time events. In drawing upon GIS (Geographic Information
Systems), this study also proposes the use of geo–tagged tweets as a sensor to detect the utilization
of space within land use in urban communities. It visualizes the spatio–temporal variation of
specific geo–located keywords in regards to diverse types of land use from a cartographic
perspective. That will help urban planners to recognize how residents are using different parts of the
urban landscape for different activities. In addition, this study aims to understand the inter–
organizational network typology of selected topics. It will use the social network analysis tools to
identify the critical links and social ties among communities in cyberspace and compare them to
realspace. Finding spatial interactions and social ties between users in social media will assist in
understanding the structure of communities and how messages or events are spread out online and

The Electrical Impulses Are Used For Stimulating The...
BACKGROUND–
AREA OF APPLICABILITY
The electrical impulses are used for stimulating the contraction of muscles which is known as
Electrical muscle stimulation (EMS), also known as neuromuscular electrical stimulation (NMES)
or electromyostimulation. The muscle tissue was stimulated by the impulses produced by a device
and were delivered on the skin through the electrodes close to the targeted stimulated muscle. These
electrodes are the pads that will adhere to the skin of the animal. The stimulated muscles contract by
the impulses which act as an action potential coming from the central nervous system. It is shown in
some studies that the electrical stimulation has the ability to increase the strength.1 Therefore, it can
be used to treat the damaged muscles. It has been utilized as a potential for strength training tool in
healthy subjects, a rehabilitation and preventive tool for patients with mobility defects, a testing tool
for the neural and/or muscular function in vivo, and a post–exercise recovery tool for athletes.2 The
number of electrical stimulation sessions varies across muscle rehabilitation protocols.3
MOTIVATION OF THE PROBLEM
In the project the paw of a mouse is stimulated by the electrical stimulation which leads to neuronal
activities within the cortex along with vascular change. The task of the project was to image the
change in neuronal activities within the cortex. With application of the electrical stimulation, the
neurons that were in the resting state

Solving proportions
Solving Proportions MATT222 Intermediate Algebra A comparison of two numbers is referred to as
a ratio, similar to fractions that can be reduced to lowest terms and then converted into a ratio of
integers. Ratios allow one to compare sizes of two quantities and unit measurements. Any statement
expressing the equality of two ratios is known as a proportion, which is used in numerous formulas
in today's real world settings and applications. Using proportions is an effective way to find
solutions by using the extreme means property or cross–multiplying. Extreme means property is
simply the end result of the product of the extremes equaling the products of the means. Cross–
multiplying is a short cut in ... Show more content on Helpwriting.net ...
x – 3 4 (x – 3) Cancel out common factors which eliminates denominator on left. y – 1 + 1 = –3x – 3
+1 To isolate y, 1 is added to both sides. Cancel common factors. y = –3 x –1 4 Equation complete
and simplified. For this equation, I could have multiplied the LCD to both sides, but I found the
extreme means property was an efficient shortcut. Cross– multiplying allowed me to eliminate the
fractions and have the same ending result. We can now consider this an extraneous solution because
the number showing as the solution but causes zero (0) in the denominator. As rational expressions
can be tricky when there is a variable involved in the denominator so caution must be adhered. The
use of proportions is everyday life and real world settings and applications are used without one
even realizing it. While proportions can determine a solution whether it be driving distance,
estimated population count, unit measuring, gas mileage, or to estimate an average time for a job to
be completed, it is a necessary tool that is used in many ways. The ratios that build the proportion
can be easily solved by cross– multiplying the extremes and means in a fast and effective way. The
wildlife can be assured that their tags will be calculated with an accurate solution for any
conservationist inquiring about a certain species. So the next time you find

Middle Childhood Essay
A.
Freeze Tag, is another variation of the game Tag. Where the person who is "it" tags everyone but
instead of being out of the game once tagged, the person will be frozen in place until another player
"un–freeze" the player, for instance by touching the frozen player on the shoulder. Freeze tag, first
begins by gathering a group of players, deciding on who is "it", determining this may be
volunteering oneself or playing a game like rock, paper, scissor. After determining the person who is
"it", he or she will count up to a number allowing the other players to scatter, giving them enough
time to get away from the person who is "it". When the person is finish counting, he/she will chase
others to tag, once they are tagged; the person is ... Show more content on Helpwriting.net ...
These formations of strategies are presented through Piaget, Concrete–Operations.
The characteristics of the Concrete operation (Lightfoot, pg397–398) that we can focus on is
identity, logical necessity, conservation, and reversibility. Identify is where children realize that even
though the child is tagged by the person who is "it" there is no addition or subtraction of anything
from the child to alter the child, personality or form. Through logical necessity they are appeared
frozen, following the rules of the game and thorough logical reasoning they are able to determine,
that they are not actually frozen but rather they are pretending to be. Children comprehends that the
person who was tagged is not actually frozen but rather they are only their appearance is view so,
acting like they are frozen shows signs of the ability to understand, conservation. They also, are able
to apply reversibility to the game, understanding that the person who is frozen can be un–frozen
after they been tagged by the person who is "it", by the same action of another player tagging them.
Their mental operation is more pronounced, capable to assess their own thoughts and is more
organized in their thought process, able to think about different solution, ideas and alternatives, as
well as the cause and effects of their actions.

Changes In Baseball
Baseball was created by Abner Doubleday in 1839. Soon after creating the sport Abner left home to
fight in the Civil War. Since then baseball has changed immensely, from the calling of strikes in
1858 to the sliding rule placed in 2016. There has many so many changes and adaptations to the
game, all for the better. Some basic rules are: the pitching rubber is 60' 6'' away from home plate,
each team has nine players on defense at a time, three strikes is an out, while four balls is a walk,
three outs is a change of sides on the inning, bases are ninety feet apart totaling three–hundred and
sixty feet around, The bat is a smooth, round stick not more than 2 3/4 inches in diameter at the
thickest part and not more than 42 inches in length. The ... Show more content on Helpwriting.net ...
If a fly ball lands on or beyond first or third base and then bounces to foul territory, it is a fair hit.
FAIR TERRITORY is that part of the playing field within, and including the first base and third
base lines, from home base to the bottom of the playing field fence and perpendicularly upwards.
All foul lines are in fair territory. Any runner is out when – He is tagged, when the ball is alive,
while off his base. EXCEPTION: A batter–runner cannot be tagged out after overrunning or over
sliding first base if he returns immediately to the base; A TAG is the action of a fielder in touching a
base with his body while holding the ball securely and firmly in his hand or glove; or touching a
runner with the ball, or with his hand or glove holding the ball, while holding the ball securely and
firmly in his hand or

Blue Shark Research Paper
Phoebe proctor
1–2 Beane
Shark Information Paper sharks "Finally, back then, it was OK to demonize an animal especially a
shark, because man had done so since the beginning of time, and, besides, sharks appeared to be
infinite in number," Peter Benchley. Sharks are interesting creatures that are being tagged for their
own protection.
Buzz has many important facts about his life you may not know. To begin they live in deep seas and
oceans close to islands and some beaches. As a result people started getting scared of endangered
blue sharks on the coast of many islands. However many people don't see them because of
environment camouflage skin when there near surface. As a result blue sharks eat many different
things such as tiny plankton, some ... Show more content on Helpwriting.net ...
To begin they live in deep seas and oceans close to islands and some beaches. As a result people
started getting scared of endangered blue sharks on the coast of many islands. However many
people don't see them because of environment camouflage skin when there near surface. As a result
blue sharks eat many different things such as tiny plankton, some fast swimming fish, mackerel, and
shrimp. Before long blue sharks get to be skilled hunters, they learn how to attack from below and
come out straight. Nevertheless they are very skilled and athletic hunters that can be very smart
when not in and inadvertence about it. At last blue sharks are very important for our

Multimedia Research Paper
4.1.1 Introduction Everything is cross–platform now. That's part of the reality that we live in – a
multifaceted, multimedia world – and I'm delighted to be a part of that. –Robert J. Sawyer After the
advent of technology and computers, lot of things around the world have changed. The way people
communicated has undergone a remarkable change thanks to automation and machines. Mobile
technology is another field which has revolutionized communication, information sharing and data
retrieval. The world of management and business was of no exception for this influence. In order to
give a proper branding and promotion for a successful business, it is customary for entrepreneurs to
get a basic understanding of Multimedia technology and which strategies ... Show more content on
Helpwriting.net ...
However, with the revolution of computers and automation speeding up, multimedia strategies are
also undergoing lot of rapid changes and improvements. There are some key advantages to
multimedia technology for advertising which are listed below: Ease of retrieval: Print media is
something which is not durable for longer periods of time. Unless properly documented, it can be
lost in time or its quality might get compromised. But with digital technology and multimedia, the
data can be stored quite intact and also be retrieved whenever needed. Speed of Transfer: Working
in multimedia increases speed of work because hundreds of copies can be easily generated with
click of a mouse. In the earlier days, printing press and mechanical technology was effectively used
for promotions. With the advent of digital printing and DTP, things have changed dramatically. High
Quality: Data generated with Multimedia is usually of high quality and the user can get creative in
building ideas as per their mindset. The variety of options available to create an attractive flyer has
become possible only due to

Ocearch And Its Effects On Sharks Essay

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