1. Course Book
PRACTICAL CELL BIOLOGY
For 2nd
Year Biology
By
Khder H. Rasul
Assistant lecturer
Department of Biology
College of science
University of Salahaddin
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2. PRACTICAL CELL BIOLOGY
Course Book for 2015-2016
Practical cell biology course book for one semester
Instructor: Khder Hussein Rasul
Scientific grade Assistant lecturer
E-mail: khder_koiss@yahoo.com
Khder.rasul@su.edu.krd
Lab time and place
• Sunday (8:30 am - 5:30 pm which include 3 groups) at lab 6.
• Monday (8:30 am - 5:30 pm which include 3 groups) at lab 6.
Course Description: This course is designed to provide information about
introduction to the cell biology, characteristics, function and parts of the cell as
well as different techniques for studying the cells. The material will be
presented at a level suitable for advanced undergraduates by lecturing,
discussion, video, power points and seminar.
References
Color atlas of cytology, histology and microscopic anatomy. 2003.
Wolfgang Kuehnel. 4th
edition.
Cell imaging techniques, methods and protocols. 2006 Humana Press
Inc. Edited by Douglas J. Taatjes and Brooke T. Mossman.
Essential cell biology.2009. Bruce Alberts , Dennis Bray, Karen Hopkin ,
Alexander Johnson , Julian Lewis , Martin Raff , Keith Roberts and
Peter Walter. 3rd
edition.
Cell and Molecular Biology, Concepts and Experiments. 2007. Gerald
Karp. 5th
edition.
Methods in Cell Biology. 2003. Edited by Leslie Wilson and Paul
Matsudaira.
The Cell a Molecular Approach. 1997. Geoffrey M. Cooper.
Internet.
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3. Course Objectives
Understand historic background of cell biology.
Become familiar with the various sub-cellular structures and organelles
inside eukaryotic cells.
To understand more advanced cellular functions, such as regulation of
membrane transport, cell signaling, cell death and cellular
differentiation.
To gain experience and understanding of microscope (light and electron
microscope).
To learn the basic histological techniques for study the tissue and cell.
To study some pathological conformational features of the cell.
Exams: Students are responsible for at least two exams and a final exam.
Exams will consist of short answer, filling the blanks, true and false, draw and
label, comment writing, and essay-style questions. Students will be examined
on material covered in class and on assigned readings.
Grading: Students grade will be based on their cumulative score from the
exams, quizzes, lab report, seminar and activity in the lab.
Sub final
* Quizzes:
Quizzes involve short exam from previous lab.
3
Practical
Laboratory (15% of total grade)
Exams(at least two) = 10%
Quizzes = 5%
Final marks
Laboratory (20% of total grade)
4. ** Seminar:
Each student will give a short seminar on a subject belong to cell biology. The
subject will be selected by the student but must receive approval from the
instructor. This subject will be graded on content as well as effectiveness.
Final Exam
Laboratory (20% of total grade)
Attendance in laboratory is graded. Attendance at lab is essential to
determine what information to learn. Occasionally, additional material will be
given out from other sources and the students will be held responsible for this
material as well. In the event of absence from lab, a student must assume full
responsibility for the material covered in lab.
Lab coat:
All students will not be allowed to enter the lab without wearing lab coat.
Course Program
Week 1
Course introduction, history of microscope discovery, Brief history of cell
discovery and cell theory.
Week 2
Introduction to Cells: prokaryote and eukaryote cells, general structure of the
animal and plant cells and shape of the cells.
Week 3
Cell dimension: ocular micrometer, stage micrometer and determine cell
dimension.
Week 4
Cell counting: to determine the abnormality or contamination. Using different
way for cell counting such as cell counting by haemocytometer and ocular grid.
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5. Week 5
Lipid Solubility of the plasma Membrane: composition of the plasma
membrane. Movement of molecules through and a cross the plasma
membrane and test different types and concentrations of alcohols on
permeability of the plasma membrane.
Week 6
Extraction of cell membrane lipid: extraction of myelin lipid from neuron.
Lab 7
Effect of various temperatures on the membrane nature and activity:
investigation the effect of different temperature on beet cell membranes.
Week 8
First exam
Lab 9
Techniques used for studying the cell, Transmission Electron
microscopy/fixation, postfixation ,dehydration, clearing, infiltration and
embedding.
Week 10
Techniques continue. Trimming, ultrasectioning and different types of sections,
mounting, glass knife, staining using heavy metals.
Week 11
Transmission electron microscope and prepare specimen with negative stain for
electron microscope.
Week 12
Mitochondria, ultrastructure, function, different shapes and size,
conformational states.
Lab 13
Isolation of mitochondria from different cell. Cells which need more energy
contain higher number of mitochondria compare to other cells which their
activity is low.
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6. Week 14
Cell death: Necrosis and programmed cell death (apoptosis), mechanism,
cellular features as revealed by LM and EM.
Week 15
Second exam
Week 16
Cellular respiration: aerobic and anaerobic respiration and cellular respiration
in yeast.
Lab 17
Testing of some stimulators and inhibitors on cellular respiration of yeast
Lab 18
Extraction of tyrosinase enzyme from potato tissue
Presentations, documents and videos are
available in the following link
http://www.slideshare.net/khder/presentations
Khder_koiss@yahoo.com
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7. Week 14
Cell death: Necrosis and programmed cell death (apoptosis), mechanism,
cellular features as revealed by LM and EM.
Week 15
Second exam
Week 16
Cellular respiration: aerobic and anaerobic respiration and cellular respiration
in yeast.
Lab 17
Testing of some stimulators and inhibitors on cellular respiration of yeast
Lab 18
Extraction of tyrosinase enzyme from potato tissue
Presentations, documents and videos are
available in the following link
http://www.slideshare.net/khder/presentations
Khder_koiss@yahoo.com
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