![EXISTING SYSTEM:
ANY basic biological logic behaviors have recently been unveiled such as
oscillator [1], toggle switch [2] and NOT, AND, OR, NAND, NOR, XOR logic
gates [3-9]. Some specific bio-combinational logic circuits and sequential logic
circuits have also been developed [3, 5, 10- 13]. In [14], a detail survey recorded
all features and improvements on a genetic circuit generating oscillatory outputs.
However, previous works in synthesizing biological circuits cannot trigger bio-
sequential logic circuits. In sequential logic circuits, synchronization of logic
reactions rely on a square wave as the clock signal. Toggle switch [2] is a
fundamental on-off switch in which two terminals with opposite level are either
connected together or disconnected from each other. However, it alone cannot
generate a periodic output signal. To the purpose, one needs to replace inducer
(IPTG, aTc) with a new input. Combining oscillator with toggle switch has been
studied in the literature [4] in which synthesis of the genetic clock based on
mixture of the multiple harmonic sine wave signals is also attempted. In this study,
we attempt to construct a genetic circuit with a simpler structure to generate
periodic square wave. The sinusoidal signal is served as the source of the input. A
dual repressor combines a toggle switch and two biological logic gates so that the
toggle switch consecutively changes its status with the output. Analysis of the](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)
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Synthesis of genetic clock with combinational biologic circuits
- 1. Synthesis of Genetic Clock with Combinational Biologic Circuits ABSTRACT: The potential of genetic clock lies in its role to triggering logic reaction for sequential biological circuits. In general, biochemical reaction of the biological system is extremely slow. However, a square wave generator used as a genetic clock the transient response should be fast enough to catch the reaction change between two logic levels. Therefore, the requirement for instantaneous changes in logic status is not likely to exist in biological systems. This paper presents a method of synthesizing a genetic clock generator based on the combination of a toggle switch with two biological logic gates. A dual repressor is used to connect the two fundamental biologic circuits. Analysis of the characteristic responses of this genetic clock with its relation to the key parameters is provided.
- 2. EXISTING SYSTEM: ANY basic biological logic behaviors have recently been unveiled such as oscillator [1], toggle switch [2] and NOT, AND, OR, NAND, NOR, XOR logic gates [3-9]. Some specific bio-combinational logic circuits and sequential logic circuits have also been developed [3, 5, 10- 13]. In [14], a detail survey recorded all features and improvements on a genetic circuit generating oscillatory outputs. However, previous works in synthesizing biological circuits cannot trigger bio- sequential logic circuits. In sequential logic circuits, synchronization of logic reactions rely on a square wave as the clock signal. Toggle switch [2] is a fundamental on-off switch in which two terminals with opposite level are either connected together or disconnected from each other. However, it alone cannot generate a periodic output signal. To the purpose, one needs to replace inducer (IPTG, aTc) with a new input. Combining oscillator with toggle switch has been studied in the literature [4] in which synthesis of the genetic clock based on mixture of the multiple harmonic sine wave signals is also attempted. In this study, we attempt to construct a genetic circuit with a simpler structure to generate periodic square wave. The sinusoidal signal is served as the source of the input. A dual repressor combines a toggle switch and two biological logic gates so that the toggle switch consecutively changes its status with the output. Analysis of the
- 3. characteristic responses with its relation to the key parameters is conducted. Our design is verified via an extensive simulation study PROPOSED SYSTEM: The structure of combining a traditional genetic oscillator with a traditional toggle switch requires at least five gene sequences (three in the former and two in the later). The choice of frequency is determined by the oscillator. The proposed gene clock with feedback structure can operate with only four gene sequences in the general situation. The oscillation frequency can be tuned through the degradation rates of the toggle switch and the biological logic gates with known parameters. The formula used to predict oscillation frequency is developed.
- 4. SOFTWARE IMPLEMENTATION: Modelsim 6.0 Xilinx 14.2 HARDWARE IMPLEMENTATION: SPARTAN-III, SPARTAN-VI