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You heated the samples containing your cheek cells. Think carefully about where this DNA was
packaged in your cells, and what high temperatures usually do to enzyme structure and function.
(Hint: Lysosomes contain enzymes known as DNAses!) What functions do you think these high
temperature incubations served in your DNA extractions?
Solution
The DNA is present in the nucleus of the cell, i.e. enclosed in the nuclear membrane. At high
temperatures, the membrane of organelles is break-down. The breakup of lysosomal membrane
result in leakage of lysosomal enzymes, which break down the other organelles. But at higher
temperatures, these enzymes become functionally inactive as they become denatured upon
exposure to higher temperatures. So, the DNAases cannot break down DNA, and it can be
extracted out.

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You heated the samples containing your cheek cells. Think carefully .pdf

  • 1. You heated the samples containing your cheek cells. Think carefully about where this DNA was packaged in your cells, and what high temperatures usually do to enzyme structure and function. (Hint: Lysosomes contain enzymes known as DNAses!) What functions do you think these high temperature incubations served in your DNA extractions? Solution The DNA is present in the nucleus of the cell, i.e. enclosed in the nuclear membrane. At high temperatures, the membrane of organelles is break-down. The breakup of lysosomal membrane result in leakage of lysosomal enzymes, which break down the other organelles. But at higher temperatures, these enzymes become functionally inactive as they become denatured upon exposure to higher temperatures. So, the DNAases cannot break down DNA, and it can be extracted out.