DNA Extraction

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DNA Extraction

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DNA Extraction

  1. 1. DNA Extraction http://www.slideshare.net/iqbal1313/dna-extraction-25521910 Muhammad Iqbal Khan SS Biology Email ID:mikhan1313@yahoo.com 0923135448175
  2. 2. MATERIALS • Dried split peas • Dishwashing detergent • Isopropyl alcohol • Meat tenderizer • Small cup or beaker • Spoon
  3. 3. Start with the peas
  4. 4. Blend the peas with water and a little salt until you have “pea soup”
  5. 5. Why do we blend the peas? • The blender separates the pea CELLS from each other
  6. 6. Put the strained pea soup in a cup Add a squirt of dishwashing detergent
  7. 7. Let the mixture sit for 5 minutes
  8. 8. What does the detergent do? • Each pea cell is surrounded by a cell membrane • DNA is in the nucleus of a cell, which is also surrounded by a membrane
  9. 9. The membranes must be broken open in order to get the DNA out of the cell
  10. 10. Detergent: • Breaks apart membranes by attaching to the lipids (fats) & proteins in the membranes
  11. 11. The cell and nuclear membranes have been broken apart, as well as all of the organelle membranes, such as those around the mitochondria and chloroplasts. So what is left? • Proteins • Carbohydrates (sugars) • DNA
  12. 12. Add a pinch of meat tenderizer to the mixture
  13. 13. Why? • Meat tenderizer is an ENZYME – A protein that helps chemical reactions happen more quickly • The enzymes in meat tenderizer break apart proteins
  14. 14. The DNA in the nucleus is surrounded by proteins
  15. 15. The meat tenderizer cuts away the proteins from the DNA
  16. 16. Stir very, very GENTLY!
  17. 17. Tilt your cup & slowly pour a layer of alcohol on top of the pea soup mixture • DNA will float up into the alcohol from the pea soup

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