10. • Choose 200 pairs 20 gr Red Tilapia (1 week)
• S. Iniae 10.000 cfu/ml challenge test (2 week)
1 month • Separate 12 pairs resistant and inresistant fish (1 week)
Identification
• Raised 12 pairs sex-separated fish, also control fish
4 month
• Mating the fish (3 weeks)
• Raise the offspring (6 weeks) and Fed S. iniae (2 weeks)
4 month • SSR test (3 weeks)
Confirmation • Data analysis (2 weeks)
@IBNU SAHIDHIR
www.artaquaculture.blogspot.com
12. 1. 200 pairs of 20 grams red tilapia are screened 6 weeks after fed with
1000 cfu/ml Streptococcus iniae.
“The symptomless seedlings (T1) are resistant and the symptoms
seedlings (T2) are inresistant . Twelve pairs are chosen from both of
them and each is sex separated into two different tank. Then, twelve
pairs additional red tilapia are added as control.”
2. After 4 months fish will be mated 4 pairs in each replication.
3. Fifty offspring (5 grams) in each replication are fed with 1000 cfu/ml
Streptococcus iniae.
4. Analysis of 50 micro-satellites markers in 48 (24 pairs) and 60
seedlings (30 in each treatment).
5. Correlation analysis between phenotypes and SSR.
Types of SSR markers linked tightly to the resistant phenotype will be
identified by GenAlEx 6.41
@IBNU SAHIDHIR
www.artaquaculture.blogspot.com
