1. USE
OF
AGRICULTURAL
WASTES
AS
SUBSTRATES
FOR
BIOREMEDIATION
WITH
TRAMETES
VERSICOLOR
Guillem
Llorens-­‐Blanch

2. BioremUAB
The
main
objecCve
of
the
research
group
is
the
development
of
novel
biological
process
to
degrade
contaminants
that
are
not
degraded
or
very
liKle.
LigninolyCc
fungi
are
used
in
our
experiments
due
to
his
unspecific
enzymaCc
system,
which
allows
the
degradaCon
of
a
wide
range
of
pollutants.

3. BioremUAB
The
main
objecCve
of
the
research
group
is
the
development
of
novel
biological
process
to
degrade
contaminants
that
are
not
degraded
or
very
liKle.
LigninolyCc
fungi
are
used
in
our
experiments
due
to
his
unspecific
enzymaCc
system,
which
allows
the
degradaCon
of
a
wide
range
of
pollutants.
Main
characteris3cs
of
Trametes
versicolor
• White-­‐rot
basidiomycetes
• Widely
distributed
in
the
environment
(lignin
degradaCon)
• Able
to
growth
in
submerged
cultures.
T.versicolor in the environment (up)
and growth in pellet form (down) • Extracellular
enzymaCc
system
with
potent
red-­‐ox
capacity:
MnP,
LiP
and
Laccase.
• Intracellular
enzymaCc
system:
Cytochrome
P450

4. Bioremedia3on
of
organic
pollutants
•
SyntheCc
dyes
Liquid
matrix
Effluents
• PharmaceuCcals
and
treatment
Personal
Care
Products
(PPCPs)
• Endocrine-­‐DisrupCng
Air
pulsed
fluidized
bed
Chemicals
(EDCs)
Bioreactor
treatment
• Polycyclic
AromaCc
Hydrocarbons
(PAHs)
• Brominated
flame
Solid
matrix
retardants
Sludge
and
soil
• Chlorinated
solvents
treatment
(PCE,
TCE)
Biopile
(up)
and
bioslurry
(down)

5. Project
DEGRAPHARMAC
•
Wastewater
form
the
University
Vila
UAB
• Wastewater
from
the
veterinary
Liquid
hospital
UAB
waste
• Wastewater
from
a
hospital
• RejecCon
stream
from
the
reverse
osmosis
process
in
a
sewage
treatment
plant
•
Sludge
from
the
acCvated
sludge
of
a
WWTP
Solid
waste
• Sludge
from
the
MBR
of
a
WWTP

6. Solid
Matrices
Matrices
Soil
Sludge

7. Solid
Matrices
Matrices
Pollutants
Soil
PAH
Spiked
(Creosote)
Spiked
Sludge
PPCP
Real
concentraCons

8. Biopiles
Contaminated
sludges
or
soils
are
mixed
with
lignocellulosic
materials
Tube
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon
Tray
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon

9. Biopiles
Contaminated
sludges
or
soils
are
mixed
with
lignocellulosic
materials
Tube
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon
Tray
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon

10. Biopiles
Contaminated
sludges
or
soils
are
mixed
with
lignocellulosic
materials
7 days
Tube
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon
Tray
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon

11. Biopiles
Contaminated
sludges
or
soils
are
mixed
with
lignocellulosic
materials
Tube
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon
Tray
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon
Addition &
Mixing

12. Biopiles
Contaminated
sludges
or
soils
are
mixed
with
lignocellulosic
materials
15 - 40
days
Tube
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon
Tray
Biopiles
Inoculum
Grown
fungus
on
Sludge/Soil
Pollutant
extracCon
the
support
addiCon

13. Biopiles
Contaminated
sludges
or
soils
are
mixed
with
lignocellulosic
materials
Parameters
Methods
Fixed
Study
Laccase
AcCvity
Humidity
Humidity
Ergosterol
QuanCficaCon
Naproxen
test
Mixture
Mixture
CO2
ProducCon
Temperature
-­‐-­‐-­‐
pH
-­‐-­‐-­‐

14. Soil
bioremedia3on.
Searching
supports
7
different
materials
were
tested
as
supports
for
soil
bioremedia3on
rabbit
wheat
rabbit
maize
wheat
rice
husks
pine
feedstock
straw
feedstock
stalks
(Pn)
straw
(Pa)
(Ar)
stardust
(C2)
pellets
(C1)
(C3)
(Pa)
Screning
based
on:
CO2
producCon Laccase
AcCvity
Ergosterol
QuanCficaCon
[mg·g-1 DW]
[U·g-1 DW]
Time
Time Time (d)
(d) (d)

15. Soil
bioremedia3on.
Searching
supports
7
different
materials
were
tested
as
supports
for
soil
bioremedia3on
rabbit
wheat
rabbit
maize
wheat
rice
husks
pine
feedstock
straw
feedstock
stalks
(Pn)
straw
(Pa)
(Ar)
stardust
(C2)
pellets
(C1)
(C3)
(Pa)
Screning
based
on:
CO2
producCon Laccase
AcCvity
Ergosterol
QuanCficaCon
[mg·g-1 DW]
[U·g-1 DW]
Time
Time Time (d)
(d) (d)

16. Soil
bioremedia3on.
Tes3ng
the
supports
Sterile
soil
Non-­‐sterile
soil

17. Soil
bioremedia3on.
Tes3ng
the
supports
Sterile
soil
Non-­‐sterile
soil
Laccase
Ac3vity
[U·∙g-­‐1
DW]
Ergosterol
[mg·∙g-­‐1
DW]
ND24
[%]

18. Soil
bioremedia3on.
Tes3ng
the
supports
Sterile
soil
Non-­‐sterile
soil
Laccase
Ac3vity
[U·∙g-­‐1
DW]
Ergosterol
[mg·∙g-­‐1
DW]
ND24
[%]

19. Soil
bioremedia3on.
Tes3ng
the
supports
Sterile
soil
Non-­‐sterile
soil
Laccase
Ac3vity
[U·∙g-­‐1
DW]
Ergosterol
[mg·∙g-­‐1
DW]
ND24
[%]

20. Soil
bioremedia3on.
Tes3ng
the
supports
Sterile
soil
Non-­‐sterile
soil
Laccase
Ac3vity
[U·∙g-­‐1
DW]
Ergosterol
[mg·∙g-­‐1
DW]
ND24
[%]

21. Soil
bioremedia3on.
Tes3ng
the
supports
rabbit
wheat
straw
rabbit
feedstock
(C2)
pellets
(C1)
feedstock
(C3)

22. Soil
bioremedia3on.
Tes3ng
the
supports
wheat
straw
pellets
(C1)

23. Soil
bioremedia3on.
PAH
removal
S:
Sterile
oil
S+bac:
Sterile
soil
with
bacterial
inoculum
NS:
Non-­‐sterile
soil

24. Sludge
bioremedia3on.
Searching
supports
3
different
materials
were
tested
as
supports
for
sludge
bioremedia3on
Pallets
chips
Olive
wood
Strain
wood
chips
chips
Screning
based
on:
• CO2
producCon
• Laccase
AcCvity
• Ergosterol
QuanCficaciton

25. Sludge
bioremedia3on.
Searching
supports
3
different
materials
were
tested
as
supports
for
sludge
bioremedia3on
Pallets
chips
Olive
wood
Strain
wood
chips
chips
Screning
based
on:
• CO2
producCon
• Laccase
AcCvity
• Ergosterol
QuanCficaciton

26. Sludge
bioremedia3on.
PPCP
degrada3on
Cultures
Characteris3cs
• Dried
MBR
sludge
• 11
days
growth
cultures
were
used
AbioCc
3g
Dry
Weight
+
1g
support
to
colonize
the
sludge
3g
DW+
1g
support
+
0.5mL
Experimental
• The
ability
of
the
fungus
to
mycelium
degrade
Hydrochlorothiazide
(HZT)
3g
DW+
1g
support
+
0.5mL
Killed
Control
mycelium
(Heat
killed)
was
studied
Laccase
AcCvity
DegradaCon
(%)

27. Sludge
bioremedia3on.
Op3mizing
the
bioremedia3on
Characteris3cs
of
the
cultures
divided
into
groups
Group
Amount
Amount
Moisture
of
of
(%)
support
sludge
(g)
(g)
1
3.0
6.0
40
2
2.5
6.5
54
3
2.0
7.0
60
4
1.3
7.7
54
5
0.8
8.2
40
6
1.3
7.7
26
7
2.0
7.0
20
8
2.5
6.5
26
9
2.0
6.0
40

28. Sludge
bioremedia3on.
PPCP
removal
DegradaCon
of
PPCP
at
real
concentraCons
in
sterile
sludge
with
Olive
wood
chips

29. THANKS
FOR
YOUR
ATTENTION