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Test of Cerebrospinal Fluid and Serous Membrane Effusion Zhongsheng Xia The Second Affiliated Hospital, Sun Yat-sen University
[object Object],[object Object]
Test of CSF ,[object Object],[object Object],[object Object]
Test of serous membrane effusion ,[object Object],[object Object]
The cerebrospinal fluid (CSF) ,[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Test of CSF
The position of lumbar puncture:  The patient is placed in lateral kneel-chest position with the neck flexed
http://www.chinavbox.cn/show/PWTVH3C3.html Video for lumbar puncture
[object Object],[object Object],[object Object],[object Object],Test of CSF
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Test of CSF
[object Object],[object Object],[object Object],Test of CSF
[object Object],[object Object],[object Object],[object Object],[object Object],Test of CSF
[object Object],[object Object],[object Object],[object Object],Test of CSF
[object Object],[object Object],[object Object],[object Object],Test of CSF
[object Object],[object Object],[object Object],[object Object],Test of CSF
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Test of CSF
Characteristic changes of CSF in common central nervous system diseases ↑   : increased;  ↓  : decreased;  ->  : normal No No virus tubercular bacillus Can be found No Pathogen   ↑  red blood cell ->  or  ↑ lymphocyte ↑  lymphocyte ↑  lymphocyte ↑  neutrophil 0~10 lymphocyte Cell count and differential  (Γ—10 6 /L) -> -> -> ↓ ↓ 119~129 Chloride (mmol/L) ↑ -> ->  or  ↑ ↓ ↓ 2.5~4.5 Glucose (mmol/L) ↑ ↑ ↑ ↑ ↑ 0.2~0.4 Protein (g/L) (quantitative) + + + + + - Protein   (qualitative) red colorless or yellow clear or mild cloudy mild cloudy cloudy clear Gross appearance ↑ ↑ ↑ ↑ ↑ 80~180 Pressure (mmH 2 O) Subarachnoid hemorrhage Brain tumor Viral meningitis Tubercular meningitis Pyogenic meningitis normal
ascites Pleural effusion Pericardial effusion
Test of serous membrane effusion ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Test of serous membrane effusion
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Test of serous membrane effusion
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Test of serous membrane effusion
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Test of serous membrane effusion
[object Object],[object Object],[object Object],[object Object],[object Object],Test of serous membrane effusion
[object Object],[object Object],[object Object],Test of serous membrane effusion
Differentiation of transudate and exudate  can be positive >0.5 >0.6 >200 IU negative <0.5 <0.6 <200 IU Pathogen Fluid/serum Protein Fluid/serum LDH LDH neutrophil, lymphocyte,etc lymphocyte Cell differential >500Γ—10 6 /L <100Γ—10 6 /L Cell count decreased Similar to serum glucose Glucose >30g/L <25 g/L Protein quantitate positive negative Rivalta test >1.018 <1.018 Gravity  cloudy clear diaphaneity yellow, red or green light yellow Gross appearance infection, malignant, etc. not from infection or tumor Main cause exudate transudate
uestions ,[object Object],[object Object],Q
CSF is a clear, colorless liquid that fills the ventricles, the subarachnoid spaces surrounding the brain and the spinal cord. Its total volume is approximately 90~150ml.
Measure pressure of CSF with manometer

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Test Of Cerebrospinal Fluid And Serous Membrance Effusion

  • 1. Test of Cerebrospinal Fluid and Serous Membrane Effusion Zhongsheng Xia The Second Affiliated Hospital, Sun Yat-sen University
  • 2.
  • 3.
  • 4.
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  • 7. The position of lumbar puncture: The patient is placed in lateral kneel-chest position with the neck flexed
  • 9.
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  • 16.
  • 17. Characteristic changes of CSF in common central nervous system diseases ↑ : increased; ↓ : decreased; -> : normal No No virus tubercular bacillus Can be found No Pathogen ↑ red blood cell -> or ↑ lymphocyte ↑ lymphocyte ↑ lymphocyte ↑ neutrophil 0~10 lymphocyte Cell count and differential (Γ—10 6 /L) -> -> -> ↓ ↓ 119~129 Chloride (mmol/L) ↑ -> -> or ↑ ↓ ↓ 2.5~4.5 Glucose (mmol/L) ↑ ↑ ↑ ↑ ↑ 0.2~0.4 Protein (g/L) (quantitative) + + + + + - Protein (qualitative) red colorless or yellow clear or mild cloudy mild cloudy cloudy clear Gross appearance ↑ ↑ ↑ ↑ ↑ 80~180 Pressure (mmH 2 O) Subarachnoid hemorrhage Brain tumor Viral meningitis Tubercular meningitis Pyogenic meningitis normal
  • 18. ascites Pleural effusion Pericardial effusion
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 26. Differentiation of transudate and exudate can be positive >0.5 >0.6 >200 IU negative <0.5 <0.6 <200 IU Pathogen Fluid/serum Protein Fluid/serum LDH LDH neutrophil, lymphocyte,etc lymphocyte Cell differential >500Γ—10 6 /L <100Γ—10 6 /L Cell count decreased Similar to serum glucose Glucose >30g/L <25 g/L Protein quantitate positive negative Rivalta test >1.018 <1.018 Gravity cloudy clear diaphaneity yellow, red or green light yellow Gross appearance infection, malignant, etc. not from infection or tumor Main cause exudate transudate
  • 27.
  • 28. CSF is a clear, colorless liquid that fills the ventricles, the subarachnoid spaces surrounding the brain and the spinal cord. Its total volume is approximately 90~150ml.
  • 29. Measure pressure of CSF with manometer