It describes how to evaluate the semen of animals such as volume, individual and mass motility, concentration, dead and live sperm, abnormality.

1. Semen Evaluation
In Animals
By
Dr.Sulake Fadhil Abbas

2. 1-Volume
The graduated collection tube use to
determine the volume of ejaculate to the nearest
0.1ml, just after collection.
2-Hydrogen ion concentration (pH)
and color:
Values of seminal pH determined by using
pH paper.

3. 3-Motility
a)Mass motility
A drop of fresh semen deposit on a
warm glass slide (37°C) and its motility
evaluate according to wave motion table under
microscope (magnification 100x)

4. b) Individual motility
Individual sperm motility evaluate by
depositing a few drops of diluted semen by
normal saline on a warm glass slide (37°C),
covered by cover slip. Evaluation make
according to progressively foreword motility.

5. 4-Dead sperms percentage:
A drop of fresh semen mix with one drop
of eosin stain then with two drops of nigrosin
stain and smear make and dried. The slide
examine under microscope (magnification
400x), uncolored sperm head (white) is
considered as life sperm, while pink head
(colored) due to dead sperm.
5-Abnormal sperms percentage:
Determination of abnormal sperm
percentage make using the same smear of live
and dead.

6. 6-Sperm concentration:
Made by adding 0.1ml of fresh semen to 19.9
ml of sperm counting solution (2.9 sodium
citrate +few drops of eosin to facilitate
distinguishing of sperm under microscope
+Mercuric chloride (HgCl2) as killing agent
for sperm). The solution components mix for
3 minutes, after that using Neubauer
chamber (RBCs square) sperms were counted
to determine sperm concentration using the
following equation:

7. C=n X200 X 400 X 10 X1000/ 80
-n=No .of sperm in five square.
-200= converse dilution rate.
-80=No .of small square in five large squares.
-10= converse depth between slide and cover
slide.
-400= converse square area of cover slide.