Why do you need to use aprotic solvents (THF, diethyl ether) in a Grignard Reaction? Solution RMgX is general representation of grignard reagent it exist as R- and MgX+ R- is very strong base. if we use any protic solvents like H2O and ROH R- abstracts proton from solvent becomes neutral R- + H2O -------------> RH so there will be reaction between grignard reagent and protoc solvent. so we must use aprotic solvent to avoid above mentioned problem..

1. Why is nucleotide synthesis an important pathway for medical intervention and what types of
disease could be treated using this information?
Solution
NUCLEOTIDES(PURINES AND PYRIMIDINES) PLAYS AN IMPORTANT ROLE IN
REPLICATING AND QUIESCENT CELLS. NUCLEOTIDES HELP IN PREVENTION OF
NEURODEGENERATION. THEY CAN BE MAINTAINED BY DE NOVO SYNTHESIS OR
SALVAGE PATHWAYS. THE NUCLEOTIDE SYNTHESIS IS IMPORTANT PATHWAY
FOR MEDICAL INTERVENTION AS IT HELP TO DEVELOP DRUG THAT CAN PLAY AS
ANALGOUE IN TREATMENT OF NUCLEOTIDE DISEASES.SALVAGE PATHWAYS
ARE IMPOTANT SOURCES OF NUCLEOTIDES FOR DNA, RNA AND ENZYME CO
FACTORS. DEGREDATION OF NUCLEOTIDES HELPS IN MAINTENANCE OF
NUCLEOIDE POOL. DISEASES DUE TO ENZYMES OF NUCLEOTIDE SYNTHESIS
COULD BE TREATED SUCH AS LESCH-NYHAN DISEASE, ATAXIA TELANGIETASIA,
OROTIC ACIDURIA, GUT,SCID,VON GIERKE DISEASE AND MANY SUCH.