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Essay on The Effects of Temperature on Beetroot Cell...
Effects of Temperature on Beetroot Cell Membranes
Background Information: A cell membranes is a thin structure that surrounds the whole cell. It contains the cytoplasm of a cell. The cell membrane is
made up of hydrophilic region and a hydrophobic region. The hydrophilic region likes water, it is on the outside of the cell, the hydrophobic region is
the inside of the cell where its protected from H2O. The cell membrane's outer surface lets larger molecules into the cell. The inner surface deals with
proteins that are important for development and cellular function. The cell membrane contains specific proteins and lipid components that enable it to
perform its roles for the cell or organelle. A cell membrane can perform many different... Show more content on Helpwriting.net ...
After 90 seconds the test tube was removed and the water in the test tube was tipped out. 10ml of tap water was then added to it and then left to stand
in the test tube rack.
The beaker was then heated to 65c. The burner was then turned off and the water's temperature was left to rise to 70c. 5 beetroot slices where then
added to the test tube labelled 70c, then water was added to the slices just to cover them. The 70c test tube was then placed into the beaker with the
water's temperature at 70c for 90 seconds. After 90 seconds the test tube was removed and the water in the test tube was tipped out. 10ml of tap water
was then added to it and then left to stand in the test tube rack.
The beaker was then heated to 75c. The burner was then turned off and the water's temperature was left to rise to 80c. 5 beetroot slices where then
added to the test tube labelled 80c, then water was added to the slices just to cover them. The 80c test tube was then placed into the beaker with the
water's temperature at 80c for 90 seconds. After 90 seconds the test tube was removed and the water in the test tube was tipped out. 10ml of tap water
was then added to it and then left to stand in the test tube rack.
After 30 minutes the test tubes were shook and then held against a white background in a well lit room. The colour of each solution was recorded.
Results:
Colour Intensity Scale 1 2
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Essay on Membranes: Cell Membrane and Purple Dark
Cell Membranes and Transport
Hands–On Labs, Inc. Version 42–0033–00–01
Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of
the experiment's questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students'
writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Exercise 1: Diffusion
Observations
Data Table 1: Rate of... Show more content on Helpwriting.net ...
Hypotonic solution.|
B. What would happen to a red blood cell placed in distilled water? Why?
Distilled water is a hypotonic solution which means in contains no solutes. According to the text (p.72), "Cells placed in a hypotonic solution plump up
rapidly as water rushes into them." This means that a RBC would eventually pop.
C. Create a graph of your experimental data by plotting the percent change in potato mass against sucrose molarity. Label the x–axis as "Molarity of
Sucrose," and the y–axis as "% Change in Mass."
D. Determine the molar concentration of the sucrose contained in the potato strips by using the graph created in question C. At the point where the
molar concentration of the sucrose in the potato would be isotonic to the sucrose, there would be no net change in the potato mass. On the graph, this
is located at the 0% change. Draw a line from this place on the y–axis to intersect the line. From the line draw a line down to the x–axis to find the
molarity.
Molar concentration of sucrose in potato = .3 M
Exercise 3: The Effect of Solvents on Membrane
System
Observations
Data Table 3: Color Intensity from Damaged Beet Cells| TestTube| Solution Treatment| ColorIntensity0– 10| Condition of Beet (turgid, flaccid, etc.)| a|
70% Isopropyl alcohol| 10| Very flacid| b| 35% Isopropyl alcohol| 7| bendable| c| 17.5% Isopropyl alcohol| 3| Less firm| d| Distilled Water| 0| Very
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Beetroot Cell Membrane Permeability Experiment
Practical Assessment
Planning
AIM
Beetroot cells contain a red pigment, which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it.
The outer of the cell is also surrounded by a membrane, which again helps contain the pigment inside the cell. In this experiment I aim to find out the
relationship between the leakage of red pigment from a beetroot cell and the surface area. To do this successfully I will need to alter the surface area of
the beetroot cells accurately and then measure if any and how much dye is let out. I can hopefully then look at my results and then find a relationship
between the two factors and be able to explain exactly why any changes took place.
PREDICTION
For ... Show more content on Helpwriting.net ...
For this reason the water used will be at room temperature (23В° approx) and will be performed on the same day to allow no margin for any
discrepancies.
Time – Time is an important factor. When leaving the beetroot in the test tubes it must be ensured that they are in the water for exactly 20 minutes
each. This will make sure that the same period of time is given for dye leakage to occur. To control this, putting the beetroot into each test tube two
minutes apart will give enough time for them to be removed before the next test tube is ready to have its contents removed.
pH – pH can affect the structure of a membrane and therefore cause it to be unable to function efficiently. The pH's, which cause this affect, would be
those that are acid or alkaline. Therefore we must use water as our substance as it has a pH 7 and this is neutral and will have no affect on the leakage
of pigment.
APPARARATUS
В·Safety goggles
В·Cutting mat
В·Test tube holder
В·5 test tubes
В·2 x 25 ml beaker
В·Raw beetroot
В·Mounted needle
В·Cork borer or scalpel
В·Stop watch
В·Colorimeter
METHOD
1.Collect all the equipment required.
2.Use a cork borer to cut cylinders of raw beetroot. Ensure that all cuts made are at a vertical angle and that each individual slice of beetroot is
exactly 3mm in thickness. Cut five discs, as this will provide a good enough range of results to draw a graph and also to make conclusions as to why the
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Advantages And Disadvantages Of Cell Membranes
Animal and plant cells are often referred to as eukaryotic cells, eukaryotic cells are cells that contain a true nucleus as well as other organelles. A
light microscope can be used in order to view the nucleus and cell membrane superficially; however, in order to view other organelles an electron
microscope is required, this is because light microscopes are unable to resolve parts of a specimen that are smaller than 0.2Вµm.
The plasma membrane, or cell membrane, is a partially permeable structure that surrounds the cell; this allows some minerals and ions to pass in and
out of the cell. When looking at the membrane microvilli can be seen, not in all cells though, these small folds found in the membrane greatly
increase the surface area of the membrane to allow substances to pass through at a greater rate. The plasma membrane can be explained and
understood in more detail by studying The Fluid Mosaic Model, the model is based on experimental observations of how membranes behave. The
Fluid Mosaic model states that membranes are made up of a phospholipid bilayer and various protein molecules.
The nucleus of the cell is the largest and heaviest organelle, the nucleus contains DNA which is needed for controlling the cell. The DNA is packaged,
by chromatin, into a small volume to allow it to fit inside the nucleus. The nucleus is surrounded by a double nuclear envelope, which contains pores,
these pores allow some molecules and ions to pass freely into and out of the nucleus as
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Cell Membrane Diffusion
The cell membrane surrounds the cytoplasm of a cell and is the barrier as to what can and cannot pass through the cell. The cell membrane acts as a
barrier and is one of the many key factors for supporting life, without it diffusion and osmosis would not be able to occur. The barrier is selectively
/partially permeable and regulates what enters and exits the cell, therefore facilitating the transport of materials needed for survival. There is two
processes in which substances are carried across the membrane, either passive transport or active transport. Passive transport transpires without the
input of cellular energy albeit Active Transport requires cells to expend energy to move the substance across the barrier. Thecell membrane also
maintains cell potential.
The images above display the cell membrane structure, the ... Show more content on Helpwriting.net ...
Equilibrium is the state of properties in a solution diffusing at an equal rate in both directions. The process is generally slow but is affected by
heat. Therefore for example, there was a glass of equal amounts cold water and another of hot, a small amount of cordial was poured into both and
the rate of diffusion was timed. You would find the hot cordial reached equilibrium must faster than the cold. This is due to atoms are driven by
kinetic energy so the rate of movement (speed) is dependent on temperature, the higher the temperature, the faster the reaction and therefore the lower
the temperature, the slower the reaction. In addition molecules will always move from condensed areas of concentration to areas of low
concentration. Simple diffusion is classified as a means of passive transport; a hydrophobic molecule will move and be able to proceed into the
hydrophobic region of the membrane without being rejected. In real life an example of diffusion being essential for survival is the respiratory
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Cell Membrane Potential Lab Report
Increase in K+ ions concentration causes increase in membrane potential in crayfish muscle cell
Abstract: The membrane potential of a cell is affected by ions and depends on the electrochemical gradient and the concentration gradient (Sandstorm et
al, 2018). There are multiple ions that affects the membrane potential including K+, Na+ and Cl– but K+ and Na+ affect the membrane potential the
most. In this experiment, we look at how K+ affects the membrane potential by changing K+ concentration levels. A similar experiment was done
years ago by Hodgkin and other researchers; we will be reproducing the results. We first used a model cell to get theoretical values and then we used
the muscle cell of a crayfish to conduct our experiment. We dissected... Show more content on Helpwriting.net ...
The electrical gradient also wants to reach equilibrium in charges so that there is less differences in charge between the inside and the outside of the
cell and the two charges cancel each other out. The equilibrium potential depends on both forces, the concentration and electrical gradient of the ions.
(Sandstrom et al, 2018). The Nernst equation is used to find the voltage at which one ion reaches its equilibrium potential and the Goldman equation is
used to find the membrane potential when there more than one ion that affects it and have different permeability.
When the membrane potential of a cell is at equilibrium, or at "rest", there is not net movement of ions across the cell membrane. At rest the
equilibrium potential of a muscle cell is between –70 mV and –90 mV due to the high concentration of K+ inside the cell. The inside of the cell is very
negative compare to the outside and when the cell depolarizes, it becomes more positive. A previous research done by P. Fatt and B. Katz show that an
increase in K+ concentration outside the cell causes the most change in resting potential (Fatt, P et al,
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Cell Membrane Essay
The normal cell membrane and the structure commonly called a "basement membrane" differ because:
0.5 points were deducted
Incorrect:
Choice A is correct because the basement membrane is where a cell attaches to the "lining", so the basement membrane will always be outside the
normal cell membrane as it is not a part of the actual enterocyte or keratinocyte cell, just where it attaches to.
7. Which of the following is an example or description of negative feedback? 0.5 points were deducted
Incorrect:
Choice B is correct because the working muscle gets hot and the blood that is being pumped into the muscles is cooler than the muscles themselves, so
blood flow into the working skeletal muscle will cool the muscle back down.
8. Which ... Show more content on Helpwriting.net ...
1.5 points were deducted
Correct:
Choice B is incorrect because too much carbohydrates lead to the cause of coliform bacteria to die, not acidophilic bacteria. Acidophilic bacteria
means acid loving bacteria, so the heightened ph would not kill them, it would make more of them grow. If more of them grow, the LPS that causes
inflammation is not circulating, because they would only circulate if the bacteria dies.
Incorrect:
Choice C is correct because acidosis degrades tight junctions between enterocytes in the colon and the cecum, which in turn lets more endotoxins from
the bacteria into the blood that causes the inflammation in laminitis.
Choice D is correct because the acidosis kills coliform bacteria, releasing LPS into the system and the LPS causes the inflammation that in turn reduces
blood flow and kills the tissues that cause laminitis.
29. Insulin resistance could increase the likelihood of laminitis because: 1.5 points were deducted
Incorrect:
Choice A is correct because the stress of too much conversion ofglucose into triglycerides can cause insulin resistance. If there are more triglycerides,
there is more fat storage within an adipocyte, causing the animal to gain weight. With more weight, there is more pressure on the structure of the
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The Membrane And The Cell Membrane
Every living thing is made up of cells. Many cells differ in size or shape and have different functions that they are required to carry out to sustain life.
A structural feature in cells is the plasma membrane, which surrounds the cell and protects it from anything dangerous around it. The plasma
membrane is able to do this because it is selectively permeable. This means that the plasma membrane decides what comes in and goes out of the cell.
This allows it to keep the important nutrients it needs as well as allowing those nutrients to enter and at the same time keeping or discarding anything
that could harm the cell (Marieb, Smith 40).
The nucleus carries the DNA and genes of the cell. The DNA basically tells the cell whatprotein to make which can affect the type of transport used.
DNA is also very important for cell division (when a cell divides to make cells identical to it). Every cell has organelles. Organelles preside in the
cytoplasm and each have a specific function. For example, the mitochondrion is an organelle where ATP is produced which is needed for active
transport.
There are two different types of processes that are used to allow substances to get through the plasma membrane. The first type is passive process
which is the differences in concentration/pressure inside and outside of the cell. The two parts of passive processes are diffusion and filtration. Diffusion
also has two different types, simple and facilitated. This is easy to remember because simple
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Why Chloride Is Important For The Transport Of Negative...
Chloride channels are a structurally diverse superfamily of transmembrane proteins that facilitate the transport of negative anions across the cell
membrane. These channels are involved in a plethora of physiological processes such as neurotransmission, excitation of skeletal, cardiac, and smooth
muscle, salt transport, cell volume regulation, and acid production in internal and external compartments. Families of these channels include the
voltage–gated CLC family, calcium–activated CaCC family, GABAA receptors, glycine receptors, and thecystic fibrosis transmembrane conductance
regulator (CFTR). CFTR is an ATP–binding cassette (ABC) transporter that is responsible for proper fluid transport across the epithelial membrane of
various cells within body tissues such as the lungs, liver, digestive tract, and reproductive tract. Mutations in the protein sequence of CFTR are
characteristic of the disease cystic fibrosis, a disease where improper or absent ion movement decreases the flow of water across exocrine epithelial
cells causing mucus and other secretions to be unusually thick.
As an ABC protein, CFTR conforms to a similar architecture as other ABC proteins. The standard ABC architecture includes 4 protein subunits, 2 of
which are transmembrane domains (TMDs) consisting of 6 О±–helices each, and an additional 2 cytoplasmic nucleotide binding domains (NBDs). The
subunits that make up most ABC proteins in humans are encoded for in a single gene, as compared to most
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Secondary Modifications Of Red Cell Membrane
Kaitlin Zoccola
Paper 10 Summary
Physiologically important secondary modifications of red cell membrane in hereditary Spherocytosis– evidence for in vivo oxidation and lipid rafts
protein variations
The experimental research published in the article, Physiologically important secondary modifications of red cell membrane in hereditary
spherocytosis– evidence for in vivo oxidation and lipid rafts protein variations by Margetis, Antonelou, Karababa, Loutradi, Margaritis, and Papassideri,
attempts to identify the relationship between the molecular mechanisms of pathogenesis and the diversity of the disorder, hereditary spherocytosis
(HS). HS is characterized by irregular morphology and shorten life span of red blood cells. The three forms of ... Show more content on Helpwriting.net
...
The biologists' motive is explained by the high number of cases around the world. In total, 1 out of every 1,000–2,500 individuals are affected by HS
and suffer from hemolysis, jaundice, and splenomegaly. The disease compromises patients' fragility and increases susceptibility to events resulting in
the rupturing of the spleen, liver failure, and kidney failure. The disease is characterized by inherited modifications to five main proteins found in the
cell membrane and the exoskeleton of red blood cells, ankyrin–1, band 3, alpha–spectrin, beta–spectrin, or/and band 4.2. Interestingly, it is believed the
same protein deficiencies can cause different levels of severity.
To study the red blood cell membrane proteins, cytoskeleton proteins, and lipid raft proteins, white ghosts and membrane ghost were prepared from the
subjects' and pateints' blood samples. The ghosts and skeletons were analyzed on SDS–PAGE Laemmli–exponential gels and immunoblots under
reducing and non–reducing environments. Oxidative data was collected from an Oxidation Detection Kit from Chemicon. Protein bands were viewed
by using digital scanning densitometry software.
Table 1, displays the densitometic analysis of membrane protein deficiencies in each of the subjects and patients as well as red blood cell counts, Hb
density, MCHC, RDW%, RET %, and bilirubin levels. From this data, the subjects were categorized according to their primary
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Cell Membranes Of Proteins And Proteins Essay
CHAPTER ONE INTRODUCTION
Cell membranes of eukaryotes are complex structures, comprised of a highly regulated heterologous distribution of lipids and proteins (Hanada, 2010).
This distribution is determined to some extent by the location and topology of lipid synthases, and results from the trafficking of proteins and lipids
(Hanada, 2010). Within the cell, transport vesicles and tubules mediate trafficking by loading desired sets of proteins at one organelle and delivering
them to the subsequent (Hanada, 2010; Kumagai et al., 2005). Lipid influx routes such as the endocytosis of membrane lipids add further to the
diversity (Hanada, 2010). The result is an asymmetric distribution of protein and lipid types across the membrane bilayer (Hanada, 2010).
Ceramides are an example of a family of cellular lipids (Yasuda et al., 2001). Ceramides are synthesised at the endoplasmic reticulum from precursor
compounds and are transported to the Golgi apparatus for conversion into one of the several sphingolipids (Yasuda et al., 2001). The transport of such
compounds is highly selective if not specific (Kumagai et al., 2005; Yasuda et al., 2001). Inhibiting transport is a useful tool in investigating the role of
substrates (Yasuda et al., 2001) and makes an attractive target for biochemical manipulation of the cell (Ueno et al., 2001).
Intracellular trafficking of ceramides is highly regulated. Two ceramide transport pathways have been identified (Kumagai et al., 2005). The first is
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Describe The Semipermeability Of The Cell Membrane
The membrane around the cells in the body is the boundary that keeps them from reaching the surroundings on the outside. It allows for certain
substances to pass through to the cells rather than others, and it contains proteins and lipids such as phospholipids (cholesterol) (Erster 3). The simplest
membranes are known as a phospholipid bilayer, given that there are two layers containing hydrophilic heads and hydrophobic tails allowing the
membrane to have its semipermeability. The tails on both layers face the inside towards each other, while the heads are on the outside (Erster 4).
They have a fluidity to it, being able to move from side to side, and rarely flip due to having to move through areas that are hydrophobic (afraid of
water) and hydrophilic (attracted to water) since it would take a lot of energy to do so (Erster 10). ... Show more content on Helpwriting.net ...
Lipid rafts are a kind of membrane domain that have a specific structure and job. The whole domain does not detach from the membrane. The closest
thing to becoming loose is when the temperature goes down, increasing the fluidity of the membrane (Erster 12,13).
B) Choice B is incorrect. These regions cannot flip inside out. In a simple membrane, the lipids move from side to side, and there can sometimes be
a flip in the bilayer between the top and bottom layer's hydrophilic heads and hydrophobic tails, but that in itself takes a lot of energy to do (as
explained above)(Erster 10). There is no way to flip the region inside out.
C) Choice C is correct. The fluidity of the membrane can change overtime depending on the temperature. As explained in my falsification of choice A,
as temperature goes down, the membrane becomes more fluid, and the tails become unsaturated to keep the phospholipids from tightening up (Erster
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Cell Membranes Of Proteins And Proteins Essay
Chapter One – Introduction
Cell membranes of eukaryotes are complex in structure, comprised of a highly regulated heterologous distribution of lipids and proteins (Hanada,
2010). This distribution is determined to some extent by the location and topology of lipid synthases, and results from the trafficking of proteins and
lipids (Hanada, 2010). Within the cell, transport vesicles and tubules mediate trafficking by loading desired sets of proteins at one organelle and
delivering them to the next (Hanada, 2010; Kumagai et al., 2005). Lipid influx routes such as the endocytosis of membrane lipids add further to the
diversity (Hanada, 2010). The result is an asymmetric distribution of protein and lipid types across the membrane phospholipid bilayer (Hanada, 2010).
Ceramides are an example of a family of cellular lipids found in the cell membrane (Yasuda et al., 2001). Ceramides are synthesised at the
endoplasmic reticulum from precursor compounds and are transported to the Golgi apparatus for conversion into one of several sphingolipids (Yasuda
et al., 2001). The transport of such compounds is highly selective if not specific (Kumagai et al., 2005; Yasuda et al., 2001). Inhibiting transport is a
useful tool in investigating the role of substrates (Yasuda et al., 2001) and makes an attractive target for biochemical manipulation of the cell (Ueno et
al., 2001).
Intracellular trafficking of ceramides is highly regulated. Twoceramide transport pathways have been identified
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Cell Membrane Permeability
Only uncharged, small, polar molecules, (such as water) and hydrophobic molecules, (such as oxygen, carbon dioxide) and lipid–soluble molecules
(such as hydrocarbons) can freely pass across the membrane. All ions and large polar molecules (such as glucose) are not permeable to the membrane.
Membrane structure
The plasma membrane maintains dynamic homeostasis by separating the internal metabolic events of the cell from its external environment and
controlling the movement of materials into and out of the cell. The membrane is a double phospholipid membrane, also referred to as a phospholipid
bilayer, and has polar hydrophilic ("water loving") phosphate heads around the outside and non polar hydrophobic ("water fearing") fatty acid tails on
the inside of the membrane. Slight variations in these structure of the fatty acids in the membrane alter the fluidity of the membrane. Phospholipids
with saturated fatty acid pack more tightly, because of the nature of their single carbon bonds. This leads to a more rigid membrane. Unsaturated fatty
acids, which have double carbon bonds, limit packing and result in a more flexible membrane. Cholesterol molecules distributed throughout the
phospholipid bilayer provide some stability to the plasma membranes of animal cells. At higher temperatures the cholesterol molecules allow the
membrane to be firmer, at lower temperatures they allow for flexibility.
Fig. 1
Proteins
The mosaic nature of the proteins scattered within the phospholipid
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The Cell Membrane Transport Lab
INTRODUCTION–
In the cell membrane transport lab, there were many experiments that were done such as osmosis, diffusion in a gel, diffusion in a liquid, diffusion in
air, and filtration, A cell membrane transport lab is done to understand the different ways of transport and why they are all important since it relates to
the human body.
Osmosis occurs when a fluid, that's most likely water, passes through a semipermeable membrane. The tonicity of the solutions on both sides of the
semipermeable membrane determines the outcome. It's possible to use osmosis to sort blood cells by using a hypotonic solution to deplete blood cells
to distinguish and sort out the differences (Parichehreh et al 2011). The tonicity of the blood and the cells can determine the health of the body, where
your body can be dehydrated to hydrate enough. Variables in this experiment is the cell when it crenates or hemolyzes until the solutions on either
sides of the semipermeable membrane reaches equilibrium. If tonicity affects osmosis, then exposing a dialysis membrane full of cytoplasmic solution
(containing glucose, albumin, NaCl, and starch) in an environment solution (deionized water) will cause the cell to swell and gain weight due to being
a hypertonic solution and will result in glucose and NaCl passing through the semipermeable membrane and leave the cell since the particles of glucose
and NaCl are small, leaving osmosis to occur until the two solutions reaches equilibrium.
In diffusion,
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The Membrane Of The Cell Membrane
1.0 Introduction
1.1 The Cell Membrane Biological cells have a membrane that separates the contents of the cell from its exterior environment and serves a number of
functions. The membrane is partially permeable, facilitating and controlling what materials pass into and out of the cell. The membrane can produce
different compartments within the cell in addition to allowing electrical signals to pass along them, playing a role in cell signalling and creating
attachment sites for enzymes and molecules involved in the metabolism (Keeton, 1983, p. 71).
1.2 The Phospholipid Bilayer
The membrane of the cell is composed of a Phospholipid Bilayer with various protein molecules floating within it phospholipids are fats and are
constructed of two fatty acid tails that are hydrophobic and a Phosphate head that is hydrophilic. The heads orientate themselves towards the water
molecules outside of the cell with the hydrophobic tails pointing away from the water molecules. A second tier of phospholipids forms a mirror image
with the heads pointing towards the water molecules inside the cell itself. These two tiers of Phospholipids form the Phospholipid Bilayer (Keeton,
1983, p. 71). Figure 1 Phospholipid Bilayer
1.3 The Fluid Mosaic Model
The Fluid Mosaic Model (Keeton, 1983, p. 71) shows that within the Phospholipid Bilayer are a number of proteins that float freely and form a 'mosaic'
pattern. These proteins can be intrinsic and exist through the complete thickness of the membrane
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The Cells And The Cell Membrane
T1.
All species of fungi, plants and animals are formed from eukaryotic cells. The Eukaryote have a true nucleus; their DNA is confined to a definite
area within the cell enclosed by a Nuclear envelope. Prokaryotes evolved before eukaryotic cells and their cells do not have a true nucleus. Prokaryote
DNA is not enclosed by a membrane.
ORGANELLESTRUCTUREFUNCTIONPLANT OR ANIMAL
NucleusWithin the cell membraneSite of the nuclear material–the DNAboth
NucleolusInside nucleusManufacture of ribosomesboth
MitochondrionNumerous in cytoplasm. Up to 1000 per cell.Aerobic respirationboth
Rough endoplasmic reticulumContinuous throughout cytoplasmIsolation and transport of newly synthesised proteinsBoth both Smooth endoplasmic ...
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These compartments allow the chemicals involved in a particular process in the cell, such as photosynthesis or respiration to be kept separate from the
rest of the cytoplasm. This cell structure and high degree of internal organisation optimizes essential chemical reactions, and allows the processes within
the cell to function. The fluid that occupies the space between the organelles is called the cytosol, a solution containing a complex mixture of enzymes,
amino acids and waste materials.
T2. The cell surface plasma membrane covers the outside of a cell, and this is a double–layered sheet of phospholipid molecules interspersed with
proteins. It separates the cell from the outside environment; this protects the cell, and allows the import and export of selected chemicals.
1Phospholipds are the main component of cell membranes. They naturally form membranes in water because they automatically arrange themselves
into a bilayer that is almost impermeable to water and to water soluble substances. The membrane keeps the cell contents in and other substances out.
There is however a need for selective transport into and out of a cell and the cell does this through membrane proteins.
2 Membrane proteins act as hydrophilic pores, water filled channels that allow water soluble substances to pass through. These pores are small and
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Why Chloride Is Important For The Transport Of Negative...
Chloride channels are a structurally diverse superfamily of transmembrane proteins that facilitate the transport of negative anions across the cell
membrane. These channels are involved in a plethora of physiological processes such as neurotransmission, excitation of skeletal, cardiac, and smooth
muscle, salt transport, cell volume regulation, and acid production in internal and external compartments. Families of these channels include the
voltage–gated CLC family, calcium–activated CaCC family, GABAA receptors, glycine receptors, and thecystic fibrosis transmembrane conductance
regulator (CFTR). CFTR is an ATP–binding cassette (ABC) transporter that is responsible for proper fluid transport across the epithelial membrane of
various cells ... Show more content on Helpwriting.net ...
In the special case of CFTR, a single gene codes for 5 domains of the 1480 amino acid glycoprotein, but homologous halves are linked by a unique
regulatory domain (R) that acts as the binding site for PKA giving the domain conformation of CFTR as TMD1–NBD1–R–NBD2–TMD2 (Gadsby et
al., 2006). For the movement of ions across the membrane, negatively charged ions, like chloride, accrue near the positively charged ends of the 2
TMDs. The flow of these ions occurs down their electrochemical gradient when the channel opens up (Linsdell, 2005). However, for this to occur,
PKA must first phosphorylate the R domain, to allow for the binding of ATP to the NBDs, nestled within a Walker A motif. Once this occurs, the 2
NBDs dimerize causing a power stroke that brings on the conformational change of the 2 TMDs and the opening of the chloride channel allowing for
the flow of ions. This conformational change is sustained until hydrolysis of one the ATPs occurs (Furukawa–Hagiya et al., 2013). A graph showing
the importance of PKA in CFTR opening and the general mechanism of the channel opening can be observed in figure–1.
The nucleotide binding domain of CFTR contains many conserved motifs found in many other ABC proteins. One of these motifs, the Walker A motif,
is the catalytic binding site for ATP. A nearby parallel О
І–sheet also contains a conserved motif, the Walker B motif. Another motif, important
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Cell Membrane And Ticket Booth
Cell Wall and the Fence
The job of the cell wall is to hold the organelles in the cell and keep it protected. The fence around the amusement park serves a similar purpose. Its job
is to keep people out of the park that do not have access. It also holds in all the rides and food stands and people in.
Cell Membrane and Ticket Booth
The job of the Cell Membrane is a passageway that lets certain small molecules, water, and carbon dioxide out. It only allows certain molecules to
pass through and a ticket booth has the same purpose. It allows people in and out once they have paid. Like the cell membrane, it only allows certain
things in and out. The ticket booth is also picky and only lets paying coustmers past .
Vacuole and Storage warehouse
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Functions Of A Cell Membrane
The cell is a basic unit of life. All living organisms are made up of cells. Cells are vital to maintain life and to allow reproduction. Each cell is
separated from its surrounding environment by a cell membrane and acts as a barrier. The cell membrane is a cell's most essential organelle. Cell
membranes are composed of four different types of molecules; phospholipids, cholesterol, proteins and carbohydrates. The main function of a cell
membrane is to control which substances can move in and out of the cell. Different organisms have different cell membranes.
The phospholipids create the basic structure of a cell membrane. A phospholipid has two separate ends; a head and a tail end. The head of the
phospholipid is known as hydrophilic, ... Show more content on Helpwriting.net ...
Diffusion only occurs when there is a concentration gradient. This means when a particular type of molecule is not spread out at an even
concentration, but either exists in a higher concentration. The concentration gradient in regards to the movement of molecules (except water) always
moves from high concentration to low concentration. Diffusion across cell membranes can occur in three main ways. The way in which it diffuses
is depended on whether the molecules are small or large, and whether the molecules have already been concentrated. When really small molecules
appear they are able to fit into a protein channel that is in the plasma membrane. This process of crossing the membrane can occur either by passive
transport of active transport. When molecules are too big to fit through any channels, then they enter the cells by moving in a vesicle or by pumping,
and this process is called active transport. When a molecule is crossing the membrane through passive transport, it is doing this because no extra
energy needs to be used to get the molecule across the membrane. However when active transport is occurring this is because extra energy needs to be
applied. Active transport can be done in two different ways, either by using pumps or using vesicles. Active transport using pumps moves small
molecules against the concentration gradient. This is why there is extra energy needed. The pumps push molecules from areas of low concentration to
high
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Cell Membranes In Red Blood Cells
11. The red blood cells (RBCs) in your body contain mainly hemoglobin (Hb), which is a large protein that cannot pass through the RBC
membrane. The RBC membrane is also impermeable to sucrose. If a red blood cell is placed into a sucrose solution in which the concentration of
sucrose on the outside of the cell is less than the concentration of the Hb on the inside, will the RBC shrink, swell up or remain the same size?
Explain your reasoning. RBCs will swell up by hypertonic, since the plasma membrane does not prevent its diffusion, water will flow into the cell by
osmosis. The cell will swell and eventually burst. The bursting of a cell is called lysis.
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
12. How does "active transport" of material ... Show more content on Helpwriting.net ...
The glucose carbon (6c) will break into two pyruvates (3C each), then one carbon will be lost in the link reaction that releases a CO2 molecule and
adds a CoA the 2 carbon molecule forming Acetyl CoA. The Acetyl CoA molecule enters the kreb Cycle. It first gets associated with oxaloacetate
(4C) that releases the CoA. Two CO2 molecules are released after the reduction of 2 NAD+ to 2 NADH. Which leaves us with a new Oxaloacetate
molecule that will enter the Kreb cycle again.
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
23. Where are the enzymes that convert pyruvate to acetyl–CoA found?
Mitochondrial matrix
Where are the enzymes of the Krebs Cycle found (i.e., in which compartment of which organelle)? Mitochondrial matrix
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
24. In aerobic respiration, does inhaled molecular oxygen (O2) combine chemically with carbon to produce CO2?
No, there is no co2 in glycolysis produced
If your answer is yes, please explain. If it is no, please
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Comparing The Structure And Function Of The Cell Membrane
Task 2.1 Describe the structure and function of the cell membrane
Every living cell is surrounded by a thin layer known as the cell membrane or plasma membrane, which controls the movement of substances in to and
out of the cell. This membrane is also responsible for maintaining the ultra–structure of the cell, and keeping its internal components together.
Organelles such as the nucleus, lysosomes, the Golgi body and mitochondria are also bound by their own individual membranes.
The molecular structure of the plasma membrane is known as the fluid mosaic model; mosaic because of the way in which proteins are scattered
around and through the membrane, and fluid because although the phospholipid bilayer forms a firm boundary around the cell, it allows molecules and
proteins to travel through it freely. ... Show more content on Helpwriting.net ...
One end of a phospholipid is hydrophilic ("water–loving") and the other end is hydrophobic ("water–fearing"). The water–soluble hydrophilic heads
form the two outer surfaces of the membrane's bilayer. One layer is aligned on the outside of the cell toward the extracellular fluid, and the other is
aligned on the inside of the membrane, toward the intracellular fluid. The water–insoluble hydrophobic tails are aligned inwards toward the middle of
the bilayer, away from any aqueous substances.
Within the phospholipid bilayer, many different types of proteins exist. These proteins are diverse in shape, size and structure. Integral proteins are
embedded within the bilayer, either partially or fully. Peripheral proteins adhere to either side of the membrane's surfaces and transmembrane proteins
extend from one side of the membrane to the
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Cell Membrane And The Border Surrounding The Cell
In an animal cell, plasma membrane is the border surrounding the cell that allows the passage of enough oxygen, nutrients, and wastes (98). Cytoplasm
is region between the cell membrane and the nucleus, and that is where we find various types of organelles of specific form and their tasks (98). The
nucleus is an information center that contains most of the genetic information (DNA) and it controls the activities by creating messenger RNA
(mRNA) so it can instruct protein synthesis (102). The nucleus's outer layer is surrounded by nuclear envelope, which is a double membrane (inner and
outer membrane (102). Furthermore, the nuclear envelope has pores that allow proteins, RNAs, etc. to enter or exit the nucleus (102). In other words, it
is like a gate that controls passage area between the nucleus and cytoplasm. Moreover, within the nucleus there is another organelle called nucleolus
where the ribosomal RNA (rRNA) is synthesized (102). Ribosomes are complexes that are made of rRNA and proteins inside of nucleolus (102–103).
They help synthesize proteins, meaning that it helps create proteins in a cell. There are two types of ribosomes: free ribosomes and bound ribosomes.
Most of the proteins are made by free ribosomes that functions within the cytoplasm (102). Whereas bound ribosomes are attached to the endoplasmic
reticulum in order to make proteins that will go into the membrane, sent within certain organelles, or export of the cell (102–104). There are two types
of
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The Membrane Of A Eukaryotic Cell
A eukaryotic cell not only has a plasma membrane as its external boundary, but it also has a variety of membranes that divide its interior into discrete
partitions, separating processes and cell mechanisms (National Center for Biotechnology Information, 2012). The complex and varied design of the
phospholipid bilayer allows the membrane to serve the variety of specific functions required by different types of cells. Moreover, one of the most
significant properties of membranes is selective permeability; permitting the passage and movement of ions and molecules to pass freely through the
membrane, but excludes others from doing so (Ek–Vitorin and Burt, 2013). One type of membrane–bound vacuole is found in plant cells, the tonoplast
is quite large and contains water (Dee, 2000). Within Beta vulgaris cells, the membrane bound vacuole also contains a red, water soluble pigment,
known as betacyanin, that provides the beetroot with its characteristic colour (Nottingham, 2004). As the pigment is water soluble, it remains in the
vacuole of the cell, however if the integrity of the membrane is disrupted (through direct chemical damage, channel damage, injury via viruses, etc.) the
contents of the vacuole will diffuse out into the surrounding environment (Andrews, Almeida and Corrotte, 2014). Through practical analysis, the
effects of various chemicals on membrane permeability in B.vulgaris was observed through the utilization of various concentrations of ethanol – 0%,
35% and 70%
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Cell Membrane Lab Report
1.The mammalian OR is part of the GPCR superfamily, while the topological distinct family of transmembrane proteins defines the insect OR. The
insect OR is comprised of a heteromeric complex of a conventional odor ligand–binding receptor and an OR83b. The N terminus for the mammalian
OR is located extracellularly while it is located intracellularly for the insect OR. The C terminus is located in the intracellularly in the mammalian OR,
while it is located extracellularly in the insect OR.
2.First, odorant compounds bind to an odorant receptor (OR) for mammals. A transduction cascade, which involves a Gprotein and AC3, is activated.
This will produce a second messenger, cyclic AMP. Because cAMP binds to cyclic nucleotide–gated (CNG) channel, cations Na+ and Ca2+ will be in
abundance. This will depolarize the cell membrane. At this stage the Ca2+ –Ca2+ can activate dependent Cl– channel as well. Olfactory sensory
neurons (OSNs) will have high concentrations of Cl– intracellularly, in which the channel will support a negatively charged Cl– efflux. This will
produce more ... Show more content on Helpwriting.net ...
A.In the experiment, with mosquitos with mutated OR co–receptor proteins and in the absence of CO2, showed that the mosquitos lost preference for
human odor treated stockings. However, when CO2 was in the test chamber, orco mutants were just as attracted to the human odor treated stockings as
the non–mutants were.
B.The results suggest that CO2 could trigger host–seeking behavior even with nonfunctioning Orco–positive neurons.
4.A pro for the usage of 2,3–butanedione and pyridine is that they could inhibit cpA neuron or activate cpA neuron respectfully. A possible con could be
the rancid butter smell of 2,3–butanedione. A definite con would be the high flammability and overall toxicity, even at low doses of pyridine. The
fish–like odor could also be considered a con. The overall stopping of the mosquito bites (2,3–butanedione) and trapping of mosquitos (pyridine) is a
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How To Move Across A Semi-Permeable Cell Membrane
The purpose of this lab is to determine which molecules can move across a semi–permeable membrane. Dialysis tubing, a thin membrane that behaves
in much the same way as a living cell's actual plasma membrane, was used to test this experiment. After filling the dialysis tubing with the starch and
glucose solutions, the artificial cell was then submerged in a water and iodine solution, and the results we're recorded after 30 minutes. The artificial
cell had gained 1 gram of the water and iodine, but had not expelled any of it's starch and glucose solution. This experiment made it easy to see which
molecules travelled and which were too big.
Introduction
Osmosis a process by which molecules pass through a semi–permeable membrane. This movement is described as movement from an area of less
concentrated solution into a more concentrated one. The hypothesis being tested states that some molecules can pass through the membrane while
other molecules cannot. If a molecule can move across the ... Show more content on Helpwriting.net ...
The dialysis tubing, pre–soaked in water, was tied at one end to form a water tight seal. After opening the other end of the tube, 2 droppers of the
starch solution and 2 droppers of the glucose solution were placed inside, before being tied off on the other end. The "cell" was weighed to
determine its density before the experiment was conducted. Distilled water was measured out into a 150mL beaker and the "cell" is placed inside.
10 drops of iodine were added to the distilled water, and the contents of the beaker became visibly yellow. The beaker was moved aside and
monitored for 30 minutes. After 30 minutes, the "cell" was removed from the liquid, dried with a paper towel, and weighed again. By weighing again,
it was determined whether or not any substances had moved in or out of the artificial plasma
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Essay about Permeability of Beetroot Cell Membranes
Permeability of Beetroot Cell Membranes
An experiment to investigate how temperature affects the
Permeability of beetroot cell membranes
Interpretation of Results:
0В°C
20В°C
30В°C
40В°C
55В°C
0.24
0.28
0.75
0.79
0.22
0.26
0.35
0.41
0.81
From 0В°C to 30В°C there is a gradual increase of absorbency which shows that as the temperature increases it is denaturing the cell wall and cell
membrane and allowing the beetroot pigment to leek out into the distilled water.
As soon as the sample of beetroot is placed into the distilled water diffusion occurs naturally, which is the net movement of molecules from a region of
high concentration (beetroot sample) to a region of low concentration (distilled water).
From ... Show more content on Helpwriting.net ...
Within my data which I collected I have found one anomaly. The anomaly is taken from my second row of results (highlighted in red).
At the temperature 40В°C, the first result I collected from this temperature was 0.75 arbitrary units and from the re–test results at the same temperature
I got 0.41 arbitrary units. This is a difference of 0.34 arbitrary units.
This piece of data is not what I would have expected due to the first set of results, although the data at 55В°C on the first and second tests are relatively
the same.
Evaluation of Practical Work:
The anomaly which I have identified at 40В°C is 0.41 arbitrary units this could be due to the size of the sample of beetroot used, if the sample was
smaller than the other samples it was have less pigment which would suggested why the result is lower than expected. Another suggestion for why the
result is lower than expected could be due to the loss of pigment in the preparation process, or from where the sample was taken from on the beetroot.
If the sample was taken from the centre of the beetroot the sample may have had more pigment contained within the cells, but if the sample was taken
from closer to the surface of the beetroot it
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Biology Exam Revision : Cell Membrane
Biology Exam Revision
Cells
Cell MembraneThe cell membrane surrounds the cytoplasm of a cell, separating its contents from the environment around it. The cell surface
membrane consists of two layers of phospholipids and regulates what can enter and leave the cell. It is a semi–permeable membrane embedded with
proteins, including receptors on the outer surface.
NucleusThe nucleus is the 'brain' of the cell. It is a large, round organelle and is surrounded by the nuclear envelope (a double membrane). The nuclear
envelope allows the exchange of materials between the nucleus and cytoplasm through its large, nuclear pores. The nucleus contains DNA therefore the
information to regulate the metabolism and reproduction for the cell. It also contains a dark staining area called the nucleolus. The nucleolus produces
ribosomes.
MitochondrionThe mitochondrion has a double membrane surrounding its ellipse shape. Some of the reactions of aerobic respiration (the process
living things go through to use food energy) occur in the mitochondrion and they are the 'powerhouses' of the cell. They produce energy in the form of
ATP.
RibosomesRibosomes are the site of protein synthesis and are made up of two subunits. One of these units is large, and the other is small. These
subunits consist of protein and ribosomal RNA. Ribosomes can be found floating free in a cell, or attached to the endoplasmic reticulum.
Rough Endoplasmic ReticulumThe rough endoplasmic reticulum is covered with
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Cell Membrane Lab Report
Cell membranes regulate the chemicals that go out or stay inside a cell. This lab tested these functions to see how the cell membrane regulates the
chemicals that pass through it. The membrane tries to achieve equilibrium on both sides by sending some chemicals to the outside or inside to get the
same amount of each chemical. Dialysis tubing was used as a cell membrane.
The cell membrane was simulated by dialysis tubing. The tubing was folded, wetted and tied to allow it to look like a cell. It was then filled в…” by
starch solution, tied shut and wetted from the outside. It was then placed into a beaker with ВЅ water and enough iodine to give the water a
yellow–type colour. Observations of the tubing were recorded at the initial stage, after 15 minutes and after a 48 hour period. ... Show more content on
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It was very easy to see that there was no colour inside the tubing but outside, it was a yellow–type colour due to the water and iodine solution. After 15
minutes, the colour of the contents in the tubing had changed. It was now a navy blue and black colour. However, this colour was mainly in the
top area of the tube (closer to the surface of the water). The solution in the beaker had also changed colour. The bolder yellow colour had changed
into a much lighter yellow. After 48 hours, the inside of the tubing had completely changed colour as the entire thing was a very navy blue and
black. The contents of the beaker had turned into a very clear substance with a yellowish colour on the bottom of the beaker. There was also a lot of
bubbles on the bottom, where the yellowish colour was, as well as near the middle of the
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Cell Membrane Structure
1 – Describe the normal structure and functioning of cell membranes, and explain how they compare to the membranes relating to a specific
abnormality in cystic fibrosis?
All cells have a cell membrane. The structure of membranes is formed from a double layer of phospholipids with proteins floating in it. The proteins
are embedded on the surface and inside or bridge the double layers of phospholipids. This structure is called a mosaic model. The main function of cell
membranes is to provide protection and support for the cell and they also control what enter and exit the cell to maintain internal balance, called
homeostasis. There are two types of a membrane protein: integral proteins and peripheral proteins. The integral membrane proteins are ... Show more
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The cystic fibrosis affects the exocrine gland, which are organs that secrete mucus. It normally produces thin, slippery secretions such as mucus, sweat,
tears and digestive juice. The organs can be affected by cystic fibrosis are sweat glands, respiratory system, digestive system and reproductive system.
Sweat glands: The individual who suffers from cystic fibrosis, the amount of body salts lost in the sweat are higher than the normal people. This can
cause problems during times of increased sweating. Such as hot weather.
Respiratory system: The lining of the respiratory tract which made of tiny, hair– like cells called the cilia. Their function is to keep the lung clean and
clear from foreign substances and mucus. The person who suffers from cystic fibrosis, the cilia can not easily remove the thick and sticky mucus out
of the air passages in the lung. The mucus clogs the air passages, causing a chronic cough and lung infections. Repeated of lung infections can lead to
damage to the lung and leading to not get enough oxygen into the body.
Digestive system: The thick and sticky mucus blocks the pancreatic ducts and preventing the enzymes from reaching in the small intestine to digest
food. The result is incomplete digestion and poor absorption of food
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Semipermeability Of The Cell Membranes
A membrane is the cell's gatekeeper. This phospholipid bilayer says what molecules can move in or out of the cell. The cell membrane is also
responsible for maintaining homeostasis. The cell membrane is semipermeable. They can control what molecules come in or out. There are two ways
that molecules can move across the membrane. Passive mechanisms like diffusion dont use any energy while active transport uses energy to move
molecules.
Diffusion is the movement of particles down their gradient. Simple Diffusion is the passive movement from high to low concentration. Facilitated
Diffusion is the movement of particles from high concentration to low concentration through a protein carrier. Osmosis is the diffusion of water across
the cell membrane. steepness of gradient, temperature, size of molecule, and distance are all factors in the rate of diffusion. Tonicity refers to the
concentration of solute in the solvent. A typical solution consists of two components known as the solvent and the solute. The solvent is used as a
dissolving medium while the solute is used as the substance dissolved in the solvent. In a hypotonic solution, there is a lower concentration of solute
relative to the inside of the cell. In a hypertonic solution, there is a high concentration of solute relative to the inside of the cell. In plants cells, the
swelling of cells placed in a hypotonic solution results in turgor pressure. The structure of the cell stops it from bursting. Turgor pressure keeps the
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Investigation Of The Cell Membrane Essay
Mustafa Elgabry
October 17, 2016
Period 3
Egg Lab
Introduction: The cell membrane benifits the cell in many different ways and is composed of many different parts which help the membrane execute
what it is supposed to practice. A model that represents these parts is called the fluid mosaic model. The model contains proteins and carbohydrates
within a phospholipid bilayer that consists of hydrophobic tails and hydrophilic heads. All of the components within the bilayer play a role in the
function of the cell membrane. For example the carbohydrates practice cell to cell recognition while the proteins transport molecules in the cell and back
out. Proteins also have alternate functions such as intercellular joining and extracellular matrix. When molecules transport in and out of the cell
through the membrane, there are two types of transport, passive and active transports. Passive transport occurs when molecules move with the
concentration gradient. One example is simple diffusion, where the molecules transport through the cell membrane effortlessly moving from a high to
low concentration. Then there is facilitated diffusion where the molecules such as glucose move with the concentration gradient but have to use a
protein to pass through the membrane. Osmosis, however, is the diffusion of water molecules across the membrane, but the water molecules also need
to pass through a protein because the inside of the lipid bilayer consists of hydrophobic tails. The purpose
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The Role Of Proteins Of Cell Membrane Transport
Discuss the role of proteins in cell membrane transport essay plan Cell membranes are surrounded by a phospholipid bilayer that provides a
semipermeable barrier for cells, separating the cytosol from the extracellular environment. Phospholipids are ampithatic, meaning that they have a
hydrophilic head and hydrophobic tail, which causes the heads to face outwards towards the water and the tails inwards, creating the bilayer [figure 1].
Small hydrophobic molecules such as O2 and CO2 and small uncharged polar molecules such as H2O and ethanol can diffuse through this bilayer,
however larger molecules and ions cannot, and thus require proteins, which are polymers of amino acids joined together by strong peptide bonds.
These proteins feature throughout the membrane, and account for around 50% of its mass [http://www.ncbi.nlm.nih.gov/books/NBK9898/] . Not only
are proteins required for transport of molecules through the membrane, but they also transport signals and are necessary for the cell support; throughout
this essay I will focus on the pivotal role they play with regards to the transport of these molecules and signals, and what occurs when these functions
are inhibited. There are two main types of membrane transport proteins: firstly, there are channel proteins, which are essentially pores that extend
across the membrane. These pores create a hydrophilic passage for molecules through he hydrophobic bilayer. The rate of movement through these
pores is significantly faster
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Cell Membrane Diffusion
Question–
How does changing a solution's concentration of solutes affect the movement of molecules across a cell membrane?
Background Research–
The cell membrane's function is to control what goes in and out of the cell. The membrane isolates the area of cytoplasm from the external environment
and regulates the exchange of entering and exiting molecules. Cell membranes communicate with other organelles to function properly.(Muskopf). A
quality of the membrane is that it is selectively permeable. This means that certain substances may pass through the membrane more easily than others.
The cell membrane consists of a phospholipid bilayer and embedded proteins.(Feldkamp, page 73). Another characteristic of the cell membrane is the
idea of passive transport. Passive transport is when certain substances inside of the cell can move throughout the membrane without the use of the cells
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The way that a molecule can move from an area of a high to low concentration is simple diffusion. Concentration gradient is the term referring to the
difference between the concentration levels. For example, when a sugar cube enters into a water beaker, the sugar sinks to the bottom and the level of
concentration increases. The concentration of the sugar molecules goes up compared to the level at the top of the water. Eventually, the sugar cube will
dissolve and the molecules diffuse, those molecules disperse throughout the water beaker.(Feldkamp, page 95). A different type of diffusion is
facilitated diffusion. This is the process in which molecules can not dissolve at a moderate rate throughout the area of the membrane. An example of
facilitated diffusion are carrier proteins. Certain molecules are not able to dissolve. Carrier proteins assist the movement of these molecules across the
membrane. These proteins move the molecules from areas of high to low concentration without the use of energy.(Feldkamp, page
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Biology : Cell Membrane Transport
Cell Membrane Transport
Ard Orpilla
Zoology 2011 Section 16
Manisha Rao
October 6, 2014 Introduction
Cells are the basic units of life. Everything alive consists of cells. In order for cells to perform their basic functions, it has to continuously seek
homeostasis. This is done by structures within the cell. A structure within the cell is the plasma membrane, which regulates what enters and exits the
cell. The cell membrane contains a phospholipid bilayer which causes the cell to have selective permeability; "allowing transport of some chemicals
but not others" ( ). Specifically, the phospholipid bilayer allows for the movement of water, oxygen, and carbon dioxide. Conversely, it is not permeable
to ions or polar chemicals. The phospholipid bilayer is in possession of hydrophilic and hydrophobic regions, which are the polar heads and tails,
respectively. This is important because the head attracts water while the tail repels water, thus regulating the substances entering and exiting the cell.
When substances pass through the plasma membrane, they use passive or active transport. Whether either transport type is used is determined by the
concentration gradient, which is the difference of concentration between areas. In an active transport, the cell must use adenosine triphosphate, or ATP,
which is produced by the mitochondria of the cell and is energy used by the body, because the substance is moving from a lower concentration gradient
to a higher
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The Effects Of Environmental Stress On Cell Membranes
Introduction:
The purpose of this experiment was to indirectly study the effects of environmental stress on cell membranes. Watercourses have been with discarding
of domestic and manufacturing, for centuries. Alarms have only escalated about the ecological alterations in the recent years of what would be the
consequences. Scientists have developed a thorough investigation in search of various man harmful waste that is damaging the environment of
watercourses.
The cell membranes are the utmost essential organelle that surrounds all living cells. Its purpose is to control what goes in and out of the cells and is
accountable for the various other properties of the cells as well. The nucleus and other organelles also have membranes that are practically
indistinguishable. Membranes are organised in a mosaic arrangement, comprised of carbohydrates, proteins and phospholipids. This can be seen in
Figure 1. The objective of this indirect examination is to study the causes of various solvents and conducts on live beetroot cells. The reason why
beetroot cells have been selected for this experiment is because they have a big membrane–bound central vacuole, as seen in Figure 2. The red colour
anthocyanin, which provides the beetroot its bright colour is located in the vacuole. The cell membrane encloses the whole beetroot cell. The
anthocyanin cannot leak out if the membranes stay unharmed. The red colour can escape if the membranes are hassled or broken.
Abstract:
Ethanol disrupts
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HM Complexation At The Cell Wall Plasma Membrane Interface...
3 HM complexation at the cell wall–plasma membrane interface
The cell wall plasma membrane (CWPs) interface is believed as a key site for the HM stress signal perception. The CWPs involved in response to
various abiotic stresses, have been extensively identified and characterised in different crop plants. Under various stress conditions, the main CWPs
include phospholipases, the salt overly sensitive kinases, transcription factors, C–repeat binding factor, dehydration sensitive element binding proteins,
mitogen activated proteins kinases and phosphatases, and abscisic acid responsive element binding factors (Ihsan et al., 2017; Dar et al., 2017). There
are known CW proteins which forms covalent bonds and undergo glycosylation with HMs some ... Show more content on Helpwriting.net ...
Various plantcell organelles are involved in metal ions' sequestration but the homeostatic role of vacuole in conferring HM stress tolerance is well
established (Bashir et al., 2016). Therefore, the central vacuole seems to be a suitable storage bucket/reservoir for excessively accumulated metal
pollutants. In fact, two vacuolar proton pumps, a vacuolar proton–ATPase (VATPase) and vacuolar proton pyrophosphates (VPPase),accelerate vacuolar
uptake of most solutes (Yan et al., 2010), which is catalyzed by either channels or transporters (Yasir et al., 2011).Few well
–characterized HM
transporter proteins include zinc–regulated transporter (ZRT), iron–regulated transporter (IRT) like protein ZIP family, ATP
–binding cassette (ABC)
transporters, the P–type metal ATPases, the natural resistance–associated macrophage protein (NRAMP) family, multidrug resistance–associated
proteins (MRP), ABC transporters of the mitochondria (ATM), cation diffusion facilitator (CDF) family of proteins, copper transporter (COPT) family
proteins, pleiotropic drug resistance (PDR) transporters, yellow–stripe–like (YSL) transporter and Ca2+ cation antiporters (CAX)(Dimkpa et al., 2015;
Taiz and Zeigher 2010). Evidence for plant vacuoles as the main reservoirs
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Investigate the Effect of Temperature on Cell Membranes...
Investigate the Effect of Temperature on Cell Membranes and Membrane Structure
If you read a recipe for cooked beetroot it will usually recommend that you don't remove the outer skin of the beetroot and don't cut off al the stalk
and root if you want to avoid getting lots of red dye in the cooking water. Beetroot contains red pigments called betalains, located within the cell
vacuole. Normally the pigments can't pass through membranes but they leak out when the beetroot is cooked.
Aim – The aim of this practical is to use beetroot to examine the effect of the temperature on cell membranes and relate the effects observed to
membrane structure. To function correctly a cell needs to be able to control transport across the... Show more content on Helpwriting.net ...
The polar heads lie at both surfaces, the kind of three–dimensional enclosed structure shown above can form. The real shape tends to be globular
(minimizing surface to volume ratio), and there are so many more bipolar molecule pairs than shown here that the radius of curvature lacks the kind of
strain and distortion shown at the corners of this drawing.
A membrane is a phospholipid bilayer studded with proteins, polysaccharides and other sorts of lipids. This patchwork of molecules is like a mosaic.
Because the molecules move about within their respective layer, the membrane behaves like a fluid. This is why the model is called the Fluid–mosaic
model.
There have been many theories as to the nature of the cell membrane.
In 1935 Daniellie and Davson put forward a possible structure, which was widely accepted. Their model was composed of a double lipid layer coated
on each side by protein. They Estimated that the membrane was so thick, with the introduction of the electron microscope, membranes could be clearly
seen for the first time. Plasma membranes of both animal and plant cells showed a characteristic three–layered
(trilarninar) appearance.
As we apply heat on to the beetroot, this denatures the proteins creating "holes" in the membrane allowing pigment to be leeched out.
The denaturing of the proteins incorporated with the added kinetic energy added to the polysaccharides allows pigment to be
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The Effect Of Temperature On The Extent Of Cell Membrane...
Purpose: To determine the effects of temperature stress on the extent of cell membrane damage.
To determine the damaging effects Acetone and Ethanol concentration on cell membrane damage.
Introduction: The cell membrane (also known as the plasma membrane) is a "thin semi–permeable membrane that surrounds the cytoplasm of a cell"
(Bailey, 2010). The purpose of cell membrane is to serve as a barrier or gate keeper. Some molecules are able to diffuse across the lipid bilayer while
others are prohibited from entering the cell. (The Editors of Encyclopædia Britannica, 2016). Allowing only specific molecules to enter the cell and
keeping others out of the cell is what gives the cell membrane the "semipermeable" characteristic (Bailey, ... Show more content on Helpwriting.net ...
This specific structure gives reason as to why temperature, acetone and ethanol affect the membrane. Temperature helps to "determine what can enter
or leave the cell and how well molecules found within the membrane can function" (Chandler, 2017). Cells function best at a temperature that
corresponds to the physiological temperature, which is 98.6В°C. An increase of temperature increases fluidity in the cell membrane. This is a result
of the fatty acid tails becoming less rigid and allowing for more movement. It leads to a change in the permeability of the cell allowing some possibly
harmful molecules in. For temperature decrease, the cell becomes less fluid and more rigid. Also affecting the permeability of the membrane,
restricting possibly important molecules from entering (Chandler, 2017). Acetone is known as an organicsolvent which causes the lipid components of
the cell membrane to dissolve leading to the breakdown of the cell membrane (Shukla, 2016). With a higher acetone concentration, results in the
increase of membrane fluidity. This is due to the "acetone molecules pushing the lipid heads apart" (Posokhov, Kyrychenko, 2013). Similarly, ethanol
is also an organic solvent. The cell membrane can not hold its shape in the presence of ethanol leading to the membrane becoming more fluid. The cell
membrane is then more permeable because of ethanol molecules penetrating the bilayer. This also has a direct
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Essay On The Effects Of Temperature On Beetroot Cell...

  • 1. Essay on The Effects of Temperature on Beetroot Cell... Effects of Temperature on Beetroot Cell Membranes Background Information: A cell membranes is a thin structure that surrounds the whole cell. It contains the cytoplasm of a cell. The cell membrane is made up of hydrophilic region and a hydrophobic region. The hydrophilic region likes water, it is on the outside of the cell, the hydrophobic region is the inside of the cell where its protected from H2O. The cell membrane's outer surface lets larger molecules into the cell. The inner surface deals with proteins that are important for development and cellular function. The cell membrane contains specific proteins and lipid components that enable it to perform its roles for the cell or organelle. A cell membrane can perform many different... Show more content on Helpwriting.net ... After 90 seconds the test tube was removed and the water in the test tube was tipped out. 10ml of tap water was then added to it and then left to stand in the test tube rack. The beaker was then heated to 65c. The burner was then turned off and the water's temperature was left to rise to 70c. 5 beetroot slices where then added to the test tube labelled 70c, then water was added to the slices just to cover them. The 70c test tube was then placed into the beaker with the water's temperature at 70c for 90 seconds. After 90 seconds the test tube was removed and the water in the test tube was tipped out. 10ml of tap water was then added to it and then left to stand in the test tube rack. The beaker was then heated to 75c. The burner was then turned off and the water's temperature was left to rise to 80c. 5 beetroot slices where then added to the test tube labelled 80c, then water was added to the slices just to cover them. The 80c test tube was then placed into the beaker with the water's temperature at 80c for 90 seconds. After 90 seconds the test tube was removed and the water in the test tube was tipped out. 10ml of tap water was then added to it and then left to stand in the test tube rack. After 30 minutes the test tubes were shook and then held against a white background in a well lit room. The colour of each solution was recorded. Results: Colour Intensity Scale 1 2
  • 2. ... Get more on HelpWriting.net ...
  • 3. Essay on Membranes: Cell Membrane and Purple Dark Cell Membranes and Transport Hands–On Labs, Inc. Version 42–0033–00–01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment's questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students' writing of lab reports by providing this information in an editable file which can be sent to an instructor. Exercise 1: Diffusion Observations Data Table 1: Rate of... Show more content on Helpwriting.net ... Hypotonic solution.| B. What would happen to a red blood cell placed in distilled water? Why? Distilled water is a hypotonic solution which means in contains no solutes. According to the text (p.72), "Cells placed in a hypotonic solution plump up rapidly as water rushes into them." This means that a RBC would eventually pop. C. Create a graph of your experimental data by plotting the percent change in potato mass against sucrose molarity. Label the x–axis as "Molarity of Sucrose," and the y–axis as "% Change in Mass." D. Determine the molar concentration of the sucrose contained in the potato strips by using the graph created in question C. At the point where the molar concentration of the sucrose in the potato would be isotonic to the sucrose, there would be no net change in the potato mass. On the graph, this is located at the 0% change. Draw a line from this place on the y–axis to intersect the line. From the line draw a line down to the x–axis to find the molarity. Molar concentration of sucrose in potato = .3 M Exercise 3: The Effect of Solvents on Membrane
  • 4. System Observations Data Table 3: Color Intensity from Damaged Beet Cells| TestTube| Solution Treatment| ColorIntensity0– 10| Condition of Beet (turgid, flaccid, etc.)| a| 70% Isopropyl alcohol| 10| Very flacid| b| 35% Isopropyl alcohol| 7| bendable| c| 17.5% Isopropyl alcohol| 3| Less firm| d| Distilled Water| 0| Very ... Get more on HelpWriting.net ...
  • 5. Beetroot Cell Membrane Permeability Experiment Practical Assessment Planning AIM Beetroot cells contain a red pigment, which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it. The outer of the cell is also surrounded by a membrane, which again helps contain the pigment inside the cell. In this experiment I aim to find out the relationship between the leakage of red pigment from a beetroot cell and the surface area. To do this successfully I will need to alter the surface area of the beetroot cells accurately and then measure if any and how much dye is let out. I can hopefully then look at my results and then find a relationship between the two factors and be able to explain exactly why any changes took place. PREDICTION For ... Show more content on Helpwriting.net ... For this reason the water used will be at room temperature (23В° approx) and will be performed on the same day to allow no margin for any discrepancies. Time – Time is an important factor. When leaving the beetroot in the test tubes it must be ensured that they are in the water for exactly 20 minutes each. This will make sure that the same period of time is given for dye leakage to occur. To control this, putting the beetroot into each test tube two minutes apart will give enough time for them to be removed before the next test tube is ready to have its contents removed. pH – pH can affect the structure of a membrane and therefore cause it to be unable to function efficiently. The pH's, which cause this affect, would be those that are acid or alkaline. Therefore we must use water as our substance as it has a pH 7 and this is neutral and will have no affect on the leakage of pigment. APPARARATUS
  • 6. В·Safety goggles В·Cutting mat В·Test tube holder В·5 test tubes В·2 x 25 ml beaker В·Raw beetroot В·Mounted needle В·Cork borer or scalpel В·Stop watch В·Colorimeter METHOD 1.Collect all the equipment required. 2.Use a cork borer to cut cylinders of raw beetroot. Ensure that all cuts made are at a vertical angle and that each individual slice of beetroot is exactly 3mm in thickness. Cut five discs, as this will provide a good enough range of results to draw a graph and also to make conclusions as to why the ... Get more on HelpWriting.net ...
  • 7. Advantages And Disadvantages Of Cell Membranes Animal and plant cells are often referred to as eukaryotic cells, eukaryotic cells are cells that contain a true nucleus as well as other organelles. A light microscope can be used in order to view the nucleus and cell membrane superficially; however, in order to view other organelles an electron microscope is required, this is because light microscopes are unable to resolve parts of a specimen that are smaller than 0.2Вµm. The plasma membrane, or cell membrane, is a partially permeable structure that surrounds the cell; this allows some minerals and ions to pass in and out of the cell. When looking at the membrane microvilli can be seen, not in all cells though, these small folds found in the membrane greatly increase the surface area of the membrane to allow substances to pass through at a greater rate. The plasma membrane can be explained and understood in more detail by studying The Fluid Mosaic Model, the model is based on experimental observations of how membranes behave. The Fluid Mosaic model states that membranes are made up of a phospholipid bilayer and various protein molecules. The nucleus of the cell is the largest and heaviest organelle, the nucleus contains DNA which is needed for controlling the cell. The DNA is packaged, by chromatin, into a small volume to allow it to fit inside the nucleus. The nucleus is surrounded by a double nuclear envelope, which contains pores, these pores allow some molecules and ions to pass freely into and out of the nucleus as ... Get more on HelpWriting.net ...
  • 8. Cell Membrane Diffusion The cell membrane surrounds the cytoplasm of a cell and is the barrier as to what can and cannot pass through the cell. The cell membrane acts as a barrier and is one of the many key factors for supporting life, without it diffusion and osmosis would not be able to occur. The barrier is selectively /partially permeable and regulates what enters and exits the cell, therefore facilitating the transport of materials needed for survival. There is two processes in which substances are carried across the membrane, either passive transport or active transport. Passive transport transpires without the input of cellular energy albeit Active Transport requires cells to expend energy to move the substance across the barrier. Thecell membrane also maintains cell potential. The images above display the cell membrane structure, the ... Show more content on Helpwriting.net ... Equilibrium is the state of properties in a solution diffusing at an equal rate in both directions. The process is generally slow but is affected by heat. Therefore for example, there was a glass of equal amounts cold water and another of hot, a small amount of cordial was poured into both and the rate of diffusion was timed. You would find the hot cordial reached equilibrium must faster than the cold. This is due to atoms are driven by kinetic energy so the rate of movement (speed) is dependent on temperature, the higher the temperature, the faster the reaction and therefore the lower the temperature, the slower the reaction. In addition molecules will always move from condensed areas of concentration to areas of low concentration. Simple diffusion is classified as a means of passive transport; a hydrophobic molecule will move and be able to proceed into the hydrophobic region of the membrane without being rejected. In real life an example of diffusion being essential for survival is the respiratory ... Get more on HelpWriting.net ...
  • 9. Cell Membrane Potential Lab Report Increase in K+ ions concentration causes increase in membrane potential in crayfish muscle cell Abstract: The membrane potential of a cell is affected by ions and depends on the electrochemical gradient and the concentration gradient (Sandstorm et al, 2018). There are multiple ions that affects the membrane potential including K+, Na+ and Cl– but K+ and Na+ affect the membrane potential the most. In this experiment, we look at how K+ affects the membrane potential by changing K+ concentration levels. A similar experiment was done years ago by Hodgkin and other researchers; we will be reproducing the results. We first used a model cell to get theoretical values and then we used the muscle cell of a crayfish to conduct our experiment. We dissected... Show more content on Helpwriting.net ... The electrical gradient also wants to reach equilibrium in charges so that there is less differences in charge between the inside and the outside of the cell and the two charges cancel each other out. The equilibrium potential depends on both forces, the concentration and electrical gradient of the ions. (Sandstrom et al, 2018). The Nernst equation is used to find the voltage at which one ion reaches its equilibrium potential and the Goldman equation is used to find the membrane potential when there more than one ion that affects it and have different permeability. When the membrane potential of a cell is at equilibrium, or at "rest", there is not net movement of ions across the cell membrane. At rest the equilibrium potential of a muscle cell is between –70 mV and –90 mV due to the high concentration of K+ inside the cell. The inside of the cell is very negative compare to the outside and when the cell depolarizes, it becomes more positive. A previous research done by P. Fatt and B. Katz show that an increase in K+ concentration outside the cell causes the most change in resting potential (Fatt, P et al, ... Get more on HelpWriting.net ...
  • 10. Cell Membrane Essay The normal cell membrane and the structure commonly called a "basement membrane" differ because: 0.5 points were deducted Incorrect: Choice A is correct because the basement membrane is where a cell attaches to the "lining", so the basement membrane will always be outside the normal cell membrane as it is not a part of the actual enterocyte or keratinocyte cell, just where it attaches to. 7. Which of the following is an example or description of negative feedback? 0.5 points were deducted Incorrect: Choice B is correct because the working muscle gets hot and the blood that is being pumped into the muscles is cooler than the muscles themselves, so blood flow into the working skeletal muscle will cool the muscle back down. 8. Which ... Show more content on Helpwriting.net ... 1.5 points were deducted Correct: Choice B is incorrect because too much carbohydrates lead to the cause of coliform bacteria to die, not acidophilic bacteria. Acidophilic bacteria means acid loving bacteria, so the heightened ph would not kill them, it would make more of them grow. If more of them grow, the LPS that causes inflammation is not circulating, because they would only circulate if the bacteria dies. Incorrect: Choice C is correct because acidosis degrades tight junctions between enterocytes in the colon and the cecum, which in turn lets more endotoxins from the bacteria into the blood that causes the inflammation in laminitis. Choice D is correct because the acidosis kills coliform bacteria, releasing LPS into the system and the LPS causes the inflammation that in turn reduces blood flow and kills the tissues that cause laminitis. 29. Insulin resistance could increase the likelihood of laminitis because: 1.5 points were deducted Incorrect: Choice A is correct because the stress of too much conversion ofglucose into triglycerides can cause insulin resistance. If there are more triglycerides, there is more fat storage within an adipocyte, causing the animal to gain weight. With more weight, there is more pressure on the structure of the
  • 11. ... Get more on HelpWriting.net ...
  • 12. The Membrane And The Cell Membrane Every living thing is made up of cells. Many cells differ in size or shape and have different functions that they are required to carry out to sustain life. A structural feature in cells is the plasma membrane, which surrounds the cell and protects it from anything dangerous around it. The plasma membrane is able to do this because it is selectively permeable. This means that the plasma membrane decides what comes in and goes out of the cell. This allows it to keep the important nutrients it needs as well as allowing those nutrients to enter and at the same time keeping or discarding anything that could harm the cell (Marieb, Smith 40). The nucleus carries the DNA and genes of the cell. The DNA basically tells the cell whatprotein to make which can affect the type of transport used. DNA is also very important for cell division (when a cell divides to make cells identical to it). Every cell has organelles. Organelles preside in the cytoplasm and each have a specific function. For example, the mitochondrion is an organelle where ATP is produced which is needed for active transport. There are two different types of processes that are used to allow substances to get through the plasma membrane. The first type is passive process which is the differences in concentration/pressure inside and outside of the cell. The two parts of passive processes are diffusion and filtration. Diffusion also has two different types, simple and facilitated. This is easy to remember because simple ... Get more on HelpWriting.net ...
  • 13. Why Chloride Is Important For The Transport Of Negative... Chloride channels are a structurally diverse superfamily of transmembrane proteins that facilitate the transport of negative anions across the cell membrane. These channels are involved in a plethora of physiological processes such as neurotransmission, excitation of skeletal, cardiac, and smooth muscle, salt transport, cell volume regulation, and acid production in internal and external compartments. Families of these channels include the voltage–gated CLC family, calcium–activated CaCC family, GABAA receptors, glycine receptors, and thecystic fibrosis transmembrane conductance regulator (CFTR). CFTR is an ATP–binding cassette (ABC) transporter that is responsible for proper fluid transport across the epithelial membrane of various cells within body tissues such as the lungs, liver, digestive tract, and reproductive tract. Mutations in the protein sequence of CFTR are characteristic of the disease cystic fibrosis, a disease where improper or absent ion movement decreases the flow of water across exocrine epithelial cells causing mucus and other secretions to be unusually thick. As an ABC protein, CFTR conforms to a similar architecture as other ABC proteins. The standard ABC architecture includes 4 protein subunits, 2 of which are transmembrane domains (TMDs) consisting of 6 О±–helices each, and an additional 2 cytoplasmic nucleotide binding domains (NBDs). The subunits that make up most ABC proteins in humans are encoded for in a single gene, as compared to most ... Get more on HelpWriting.net ...
  • 14. Secondary Modifications Of Red Cell Membrane Kaitlin Zoccola Paper 10 Summary Physiologically important secondary modifications of red cell membrane in hereditary Spherocytosis– evidence for in vivo oxidation and lipid rafts protein variations The experimental research published in the article, Physiologically important secondary modifications of red cell membrane in hereditary spherocytosis– evidence for in vivo oxidation and lipid rafts protein variations by Margetis, Antonelou, Karababa, Loutradi, Margaritis, and Papassideri, attempts to identify the relationship between the molecular mechanisms of pathogenesis and the diversity of the disorder, hereditary spherocytosis (HS). HS is characterized by irregular morphology and shorten life span of red blood cells. The three forms of ... Show more content on Helpwriting.net ... The biologists' motive is explained by the high number of cases around the world. In total, 1 out of every 1,000–2,500 individuals are affected by HS and suffer from hemolysis, jaundice, and splenomegaly. The disease compromises patients' fragility and increases susceptibility to events resulting in the rupturing of the spleen, liver failure, and kidney failure. The disease is characterized by inherited modifications to five main proteins found in the cell membrane and the exoskeleton of red blood cells, ankyrin–1, band 3, alpha–spectrin, beta–spectrin, or/and band 4.2. Interestingly, it is believed the same protein deficiencies can cause different levels of severity. To study the red blood cell membrane proteins, cytoskeleton proteins, and lipid raft proteins, white ghosts and membrane ghost were prepared from the subjects' and pateints' blood samples. The ghosts and skeletons were analyzed on SDS–PAGE Laemmli–exponential gels and immunoblots under reducing and non–reducing environments. Oxidative data was collected from an Oxidation Detection Kit from Chemicon. Protein bands were viewed by using digital scanning densitometry software. Table 1, displays the densitometic analysis of membrane protein deficiencies in each of the subjects and patients as well as red blood cell counts, Hb density, MCHC, RDW%, RET %, and bilirubin levels. From this data, the subjects were categorized according to their primary ... Get more on HelpWriting.net ...
  • 15. Cell Membranes Of Proteins And Proteins Essay CHAPTER ONE INTRODUCTION Cell membranes of eukaryotes are complex structures, comprised of a highly regulated heterologous distribution of lipids and proteins (Hanada, 2010). This distribution is determined to some extent by the location and topology of lipid synthases, and results from the trafficking of proteins and lipids (Hanada, 2010). Within the cell, transport vesicles and tubules mediate trafficking by loading desired sets of proteins at one organelle and delivering them to the subsequent (Hanada, 2010; Kumagai et al., 2005). Lipid influx routes such as the endocytosis of membrane lipids add further to the diversity (Hanada, 2010). The result is an asymmetric distribution of protein and lipid types across the membrane bilayer (Hanada, 2010). Ceramides are an example of a family of cellular lipids (Yasuda et al., 2001). Ceramides are synthesised at the endoplasmic reticulum from precursor compounds and are transported to the Golgi apparatus for conversion into one of the several sphingolipids (Yasuda et al., 2001). The transport of such compounds is highly selective if not specific (Kumagai et al., 2005; Yasuda et al., 2001). Inhibiting transport is a useful tool in investigating the role of substrates (Yasuda et al., 2001) and makes an attractive target for biochemical manipulation of the cell (Ueno et al., 2001). Intracellular trafficking of ceramides is highly regulated. Two ceramide transport pathways have been identified (Kumagai et al., 2005). The first is ... Get more on HelpWriting.net ...
  • 16. Describe The Semipermeability Of The Cell Membrane The membrane around the cells in the body is the boundary that keeps them from reaching the surroundings on the outside. It allows for certain substances to pass through to the cells rather than others, and it contains proteins and lipids such as phospholipids (cholesterol) (Erster 3). The simplest membranes are known as a phospholipid bilayer, given that there are two layers containing hydrophilic heads and hydrophobic tails allowing the membrane to have its semipermeability. The tails on both layers face the inside towards each other, while the heads are on the outside (Erster 4). They have a fluidity to it, being able to move from side to side, and rarely flip due to having to move through areas that are hydrophobic (afraid of water) and hydrophilic (attracted to water) since it would take a lot of energy to do so (Erster 10). ... Show more content on Helpwriting.net ... Lipid rafts are a kind of membrane domain that have a specific structure and job. The whole domain does not detach from the membrane. The closest thing to becoming loose is when the temperature goes down, increasing the fluidity of the membrane (Erster 12,13). B) Choice B is incorrect. These regions cannot flip inside out. In a simple membrane, the lipids move from side to side, and there can sometimes be a flip in the bilayer between the top and bottom layer's hydrophilic heads and hydrophobic tails, but that in itself takes a lot of energy to do (as explained above)(Erster 10). There is no way to flip the region inside out. C) Choice C is correct. The fluidity of the membrane can change overtime depending on the temperature. As explained in my falsification of choice A, as temperature goes down, the membrane becomes more fluid, and the tails become unsaturated to keep the phospholipids from tightening up (Erster ... Get more on HelpWriting.net ...
  • 17. Cell Membranes Of Proteins And Proteins Essay Chapter One – Introduction Cell membranes of eukaryotes are complex in structure, comprised of a highly regulated heterologous distribution of lipids and proteins (Hanada, 2010). This distribution is determined to some extent by the location and topology of lipid synthases, and results from the trafficking of proteins and lipids (Hanada, 2010). Within the cell, transport vesicles and tubules mediate trafficking by loading desired sets of proteins at one organelle and delivering them to the next (Hanada, 2010; Kumagai et al., 2005). Lipid influx routes such as the endocytosis of membrane lipids add further to the diversity (Hanada, 2010). The result is an asymmetric distribution of protein and lipid types across the membrane phospholipid bilayer (Hanada, 2010). Ceramides are an example of a family of cellular lipids found in the cell membrane (Yasuda et al., 2001). Ceramides are synthesised at the endoplasmic reticulum from precursor compounds and are transported to the Golgi apparatus for conversion into one of several sphingolipids (Yasuda et al., 2001). The transport of such compounds is highly selective if not specific (Kumagai et al., 2005; Yasuda et al., 2001). Inhibiting transport is a useful tool in investigating the role of substrates (Yasuda et al., 2001) and makes an attractive target for biochemical manipulation of the cell (Ueno et al., 2001). Intracellular trafficking of ceramides is highly regulated. Twoceramide transport pathways have been identified ... Get more on HelpWriting.net ...
  • 18. Cell Membrane Permeability Only uncharged, small, polar molecules, (such as water) and hydrophobic molecules, (such as oxygen, carbon dioxide) and lipid–soluble molecules (such as hydrocarbons) can freely pass across the membrane. All ions and large polar molecules (such as glucose) are not permeable to the membrane. Membrane structure The plasma membrane maintains dynamic homeostasis by separating the internal metabolic events of the cell from its external environment and controlling the movement of materials into and out of the cell. The membrane is a double phospholipid membrane, also referred to as a phospholipid bilayer, and has polar hydrophilic ("water loving") phosphate heads around the outside and non polar hydrophobic ("water fearing") fatty acid tails on the inside of the membrane. Slight variations in these structure of the fatty acids in the membrane alter the fluidity of the membrane. Phospholipids with saturated fatty acid pack more tightly, because of the nature of their single carbon bonds. This leads to a more rigid membrane. Unsaturated fatty acids, which have double carbon bonds, limit packing and result in a more flexible membrane. Cholesterol molecules distributed throughout the phospholipid bilayer provide some stability to the plasma membranes of animal cells. At higher temperatures the cholesterol molecules allow the membrane to be firmer, at lower temperatures they allow for flexibility. Fig. 1 Proteins The mosaic nature of the proteins scattered within the phospholipid ... Get more on HelpWriting.net ...
  • 19. The Cell Membrane Transport Lab INTRODUCTION– In the cell membrane transport lab, there were many experiments that were done such as osmosis, diffusion in a gel, diffusion in a liquid, diffusion in air, and filtration, A cell membrane transport lab is done to understand the different ways of transport and why they are all important since it relates to the human body. Osmosis occurs when a fluid, that's most likely water, passes through a semipermeable membrane. The tonicity of the solutions on both sides of the semipermeable membrane determines the outcome. It's possible to use osmosis to sort blood cells by using a hypotonic solution to deplete blood cells to distinguish and sort out the differences (Parichehreh et al 2011). The tonicity of the blood and the cells can determine the health of the body, where your body can be dehydrated to hydrate enough. Variables in this experiment is the cell when it crenates or hemolyzes until the solutions on either sides of the semipermeable membrane reaches equilibrium. If tonicity affects osmosis, then exposing a dialysis membrane full of cytoplasmic solution (containing glucose, albumin, NaCl, and starch) in an environment solution (deionized water) will cause the cell to swell and gain weight due to being a hypertonic solution and will result in glucose and NaCl passing through the semipermeable membrane and leave the cell since the particles of glucose and NaCl are small, leaving osmosis to occur until the two solutions reaches equilibrium. In diffusion, ... Get more on HelpWriting.net ...
  • 20. The Membrane Of The Cell Membrane 1.0 Introduction 1.1 The Cell Membrane Biological cells have a membrane that separates the contents of the cell from its exterior environment and serves a number of functions. The membrane is partially permeable, facilitating and controlling what materials pass into and out of the cell. The membrane can produce different compartments within the cell in addition to allowing electrical signals to pass along them, playing a role in cell signalling and creating attachment sites for enzymes and molecules involved in the metabolism (Keeton, 1983, p. 71). 1.2 The Phospholipid Bilayer The membrane of the cell is composed of a Phospholipid Bilayer with various protein molecules floating within it phospholipids are fats and are constructed of two fatty acid tails that are hydrophobic and a Phosphate head that is hydrophilic. The heads orientate themselves towards the water molecules outside of the cell with the hydrophobic tails pointing away from the water molecules. A second tier of phospholipids forms a mirror image with the heads pointing towards the water molecules inside the cell itself. These two tiers of Phospholipids form the Phospholipid Bilayer (Keeton, 1983, p. 71). Figure 1 Phospholipid Bilayer 1.3 The Fluid Mosaic Model The Fluid Mosaic Model (Keeton, 1983, p. 71) shows that within the Phospholipid Bilayer are a number of proteins that float freely and form a 'mosaic' pattern. These proteins can be intrinsic and exist through the complete thickness of the membrane ... Get more on HelpWriting.net ...
  • 21. The Cells And The Cell Membrane T1. All species of fungi, plants and animals are formed from eukaryotic cells. The Eukaryote have a true nucleus; their DNA is confined to a definite area within the cell enclosed by a Nuclear envelope. Prokaryotes evolved before eukaryotic cells and their cells do not have a true nucleus. Prokaryote DNA is not enclosed by a membrane. ORGANELLESTRUCTUREFUNCTIONPLANT OR ANIMAL NucleusWithin the cell membraneSite of the nuclear material–the DNAboth NucleolusInside nucleusManufacture of ribosomesboth MitochondrionNumerous in cytoplasm. Up to 1000 per cell.Aerobic respirationboth Rough endoplasmic reticulumContinuous throughout cytoplasmIsolation and transport of newly synthesised proteinsBoth both Smooth endoplasmic ... Show more content on Helpwriting.net ... These compartments allow the chemicals involved in a particular process in the cell, such as photosynthesis or respiration to be kept separate from the rest of the cytoplasm. This cell structure and high degree of internal organisation optimizes essential chemical reactions, and allows the processes within the cell to function. The fluid that occupies the space between the organelles is called the cytosol, a solution containing a complex mixture of enzymes, amino acids and waste materials. T2. The cell surface plasma membrane covers the outside of a cell, and this is a double–layered sheet of phospholipid molecules interspersed with proteins. It separates the cell from the outside environment; this protects the cell, and allows the import and export of selected chemicals. 1Phospholipds are the main component of cell membranes. They naturally form membranes in water because they automatically arrange themselves into a bilayer that is almost impermeable to water and to water soluble substances. The membrane keeps the cell contents in and other substances out. There is however a need for selective transport into and out of a cell and the cell does this through membrane proteins. 2 Membrane proteins act as hydrophilic pores, water filled channels that allow water soluble substances to pass through. These pores are small and
  • 22. ... Get more on HelpWriting.net ...
  • 23. Why Chloride Is Important For The Transport Of Negative... Chloride channels are a structurally diverse superfamily of transmembrane proteins that facilitate the transport of negative anions across the cell membrane. These channels are involved in a plethora of physiological processes such as neurotransmission, excitation of skeletal, cardiac, and smooth muscle, salt transport, cell volume regulation, and acid production in internal and external compartments. Families of these channels include the voltage–gated CLC family, calcium–activated CaCC family, GABAA receptors, glycine receptors, and thecystic fibrosis transmembrane conductance regulator (CFTR). CFTR is an ATP–binding cassette (ABC) transporter that is responsible for proper fluid transport across the epithelial membrane of various cells ... Show more content on Helpwriting.net ... In the special case of CFTR, a single gene codes for 5 domains of the 1480 amino acid glycoprotein, but homologous halves are linked by a unique regulatory domain (R) that acts as the binding site for PKA giving the domain conformation of CFTR as TMD1–NBD1–R–NBD2–TMD2 (Gadsby et al., 2006). For the movement of ions across the membrane, negatively charged ions, like chloride, accrue near the positively charged ends of the 2 TMDs. The flow of these ions occurs down their electrochemical gradient when the channel opens up (Linsdell, 2005). However, for this to occur, PKA must first phosphorylate the R domain, to allow for the binding of ATP to the NBDs, nestled within a Walker A motif. Once this occurs, the 2 NBDs dimerize causing a power stroke that brings on the conformational change of the 2 TMDs and the opening of the chloride channel allowing for the flow of ions. This conformational change is sustained until hydrolysis of one the ATPs occurs (Furukawa–Hagiya et al., 2013). A graph showing the importance of PKA in CFTR opening and the general mechanism of the channel opening can be observed in figure–1. The nucleotide binding domain of CFTR contains many conserved motifs found in many other ABC proteins. One of these motifs, the Walker A motif, is the catalytic binding site for ATP. A nearby parallel О І–sheet also contains a conserved motif, the Walker B motif. Another motif, important ... Get more on HelpWriting.net ...
  • 24. Cell Membrane And Ticket Booth Cell Wall and the Fence The job of the cell wall is to hold the organelles in the cell and keep it protected. The fence around the amusement park serves a similar purpose. Its job is to keep people out of the park that do not have access. It also holds in all the rides and food stands and people in. Cell Membrane and Ticket Booth The job of the Cell Membrane is a passageway that lets certain small molecules, water, and carbon dioxide out. It only allows certain molecules to pass through and a ticket booth has the same purpose. It allows people in and out once they have paid. Like the cell membrane, it only allows certain things in and out. The ticket booth is also picky and only lets paying coustmers past . Vacuole and Storage warehouse ... Get more on HelpWriting.net ...
  • 25. Functions Of A Cell Membrane The cell is a basic unit of life. All living organisms are made up of cells. Cells are vital to maintain life and to allow reproduction. Each cell is separated from its surrounding environment by a cell membrane and acts as a barrier. The cell membrane is a cell's most essential organelle. Cell membranes are composed of four different types of molecules; phospholipids, cholesterol, proteins and carbohydrates. The main function of a cell membrane is to control which substances can move in and out of the cell. Different organisms have different cell membranes. The phospholipids create the basic structure of a cell membrane. A phospholipid has two separate ends; a head and a tail end. The head of the phospholipid is known as hydrophilic, ... Show more content on Helpwriting.net ... Diffusion only occurs when there is a concentration gradient. This means when a particular type of molecule is not spread out at an even concentration, but either exists in a higher concentration. The concentration gradient in regards to the movement of molecules (except water) always moves from high concentration to low concentration. Diffusion across cell membranes can occur in three main ways. The way in which it diffuses is depended on whether the molecules are small or large, and whether the molecules have already been concentrated. When really small molecules appear they are able to fit into a protein channel that is in the plasma membrane. This process of crossing the membrane can occur either by passive transport of active transport. When molecules are too big to fit through any channels, then they enter the cells by moving in a vesicle or by pumping, and this process is called active transport. When a molecule is crossing the membrane through passive transport, it is doing this because no extra energy needs to be used to get the molecule across the membrane. However when active transport is occurring this is because extra energy needs to be applied. Active transport can be done in two different ways, either by using pumps or using vesicles. Active transport using pumps moves small molecules against the concentration gradient. This is why there is extra energy needed. The pumps push molecules from areas of low concentration to high ... Get more on HelpWriting.net ...
  • 26. Cell Membranes In Red Blood Cells 11. The red blood cells (RBCs) in your body contain mainly hemoglobin (Hb), which is a large protein that cannot pass through the RBC membrane. The RBC membrane is also impermeable to sucrose. If a red blood cell is placed into a sucrose solution in which the concentration of sucrose on the outside of the cell is less than the concentration of the Hb on the inside, will the RBC shrink, swell up or remain the same size? Explain your reasoning. RBCs will swell up by hypertonic, since the plasma membrane does not prevent its diffusion, water will flow into the cell by osmosis. The cell will swell and eventually burst. The bursting of a cell is called lysis. ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ 12. How does "active transport" of material ... Show more content on Helpwriting.net ... The glucose carbon (6c) will break into two pyruvates (3C each), then one carbon will be lost in the link reaction that releases a CO2 molecule and adds a CoA the 2 carbon molecule forming Acetyl CoA. The Acetyl CoA molecule enters the kreb Cycle. It first gets associated with oxaloacetate (4C) that releases the CoA. Two CO2 molecules are released after the reduction of 2 NAD+ to 2 NADH. Which leaves us with a new Oxaloacetate molecule that will enter the Kreb cycle again. ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ 23. Where are the enzymes that convert pyruvate to acetyl–CoA found? Mitochondrial matrix Where are the enzymes of the Krebs Cycle found (i.e., in which compartment of which organelle)? Mitochondrial matrix ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ 24. In aerobic respiration, does inhaled molecular oxygen (O2) combine chemically with carbon to produce CO2? No, there is no co2 in glycolysis produced If your answer is yes, please explain. If it is no, please
  • 27. ... Get more on HelpWriting.net ...
  • 28. Comparing The Structure And Function Of The Cell Membrane Task 2.1 Describe the structure and function of the cell membrane Every living cell is surrounded by a thin layer known as the cell membrane or plasma membrane, which controls the movement of substances in to and out of the cell. This membrane is also responsible for maintaining the ultra–structure of the cell, and keeping its internal components together. Organelles such as the nucleus, lysosomes, the Golgi body and mitochondria are also bound by their own individual membranes. The molecular structure of the plasma membrane is known as the fluid mosaic model; mosaic because of the way in which proteins are scattered around and through the membrane, and fluid because although the phospholipid bilayer forms a firm boundary around the cell, it allows molecules and proteins to travel through it freely. ... Show more content on Helpwriting.net ... One end of a phospholipid is hydrophilic ("water–loving") and the other end is hydrophobic ("water–fearing"). The water–soluble hydrophilic heads form the two outer surfaces of the membrane's bilayer. One layer is aligned on the outside of the cell toward the extracellular fluid, and the other is aligned on the inside of the membrane, toward the intracellular fluid. The water–insoluble hydrophobic tails are aligned inwards toward the middle of the bilayer, away from any aqueous substances. Within the phospholipid bilayer, many different types of proteins exist. These proteins are diverse in shape, size and structure. Integral proteins are embedded within the bilayer, either partially or fully. Peripheral proteins adhere to either side of the membrane's surfaces and transmembrane proteins extend from one side of the membrane to the ... Get more on HelpWriting.net ...
  • 29. Cell Membrane And The Border Surrounding The Cell In an animal cell, plasma membrane is the border surrounding the cell that allows the passage of enough oxygen, nutrients, and wastes (98). Cytoplasm is region between the cell membrane and the nucleus, and that is where we find various types of organelles of specific form and their tasks (98). The nucleus is an information center that contains most of the genetic information (DNA) and it controls the activities by creating messenger RNA (mRNA) so it can instruct protein synthesis (102). The nucleus's outer layer is surrounded by nuclear envelope, which is a double membrane (inner and outer membrane (102). Furthermore, the nuclear envelope has pores that allow proteins, RNAs, etc. to enter or exit the nucleus (102). In other words, it is like a gate that controls passage area between the nucleus and cytoplasm. Moreover, within the nucleus there is another organelle called nucleolus where the ribosomal RNA (rRNA) is synthesized (102). Ribosomes are complexes that are made of rRNA and proteins inside of nucleolus (102–103). They help synthesize proteins, meaning that it helps create proteins in a cell. There are two types of ribosomes: free ribosomes and bound ribosomes. Most of the proteins are made by free ribosomes that functions within the cytoplasm (102). Whereas bound ribosomes are attached to the endoplasmic reticulum in order to make proteins that will go into the membrane, sent within certain organelles, or export of the cell (102–104). There are two types of ... Get more on HelpWriting.net ...
  • 30. The Membrane Of A Eukaryotic Cell A eukaryotic cell not only has a plasma membrane as its external boundary, but it also has a variety of membranes that divide its interior into discrete partitions, separating processes and cell mechanisms (National Center for Biotechnology Information, 2012). The complex and varied design of the phospholipid bilayer allows the membrane to serve the variety of specific functions required by different types of cells. Moreover, one of the most significant properties of membranes is selective permeability; permitting the passage and movement of ions and molecules to pass freely through the membrane, but excludes others from doing so (Ek–Vitorin and Burt, 2013). One type of membrane–bound vacuole is found in plant cells, the tonoplast is quite large and contains water (Dee, 2000). Within Beta vulgaris cells, the membrane bound vacuole also contains a red, water soluble pigment, known as betacyanin, that provides the beetroot with its characteristic colour (Nottingham, 2004). As the pigment is water soluble, it remains in the vacuole of the cell, however if the integrity of the membrane is disrupted (through direct chemical damage, channel damage, injury via viruses, etc.) the contents of the vacuole will diffuse out into the surrounding environment (Andrews, Almeida and Corrotte, 2014). Through practical analysis, the effects of various chemicals on membrane permeability in B.vulgaris was observed through the utilization of various concentrations of ethanol – 0%, 35% and 70% ... Get more on HelpWriting.net ...
  • 31. Cell Membrane Lab Report 1.The mammalian OR is part of the GPCR superfamily, while the topological distinct family of transmembrane proteins defines the insect OR. The insect OR is comprised of a heteromeric complex of a conventional odor ligand–binding receptor and an OR83b. The N terminus for the mammalian OR is located extracellularly while it is located intracellularly for the insect OR. The C terminus is located in the intracellularly in the mammalian OR, while it is located extracellularly in the insect OR. 2.First, odorant compounds bind to an odorant receptor (OR) for mammals. A transduction cascade, which involves a Gprotein and AC3, is activated. This will produce a second messenger, cyclic AMP. Because cAMP binds to cyclic nucleotide–gated (CNG) channel, cations Na+ and Ca2+ will be in abundance. This will depolarize the cell membrane. At this stage the Ca2+ –Ca2+ can activate dependent Cl– channel as well. Olfactory sensory neurons (OSNs) will have high concentrations of Cl– intracellularly, in which the channel will support a negatively charged Cl– efflux. This will produce more ... Show more content on Helpwriting.net ... A.In the experiment, with mosquitos with mutated OR co–receptor proteins and in the absence of CO2, showed that the mosquitos lost preference for human odor treated stockings. However, when CO2 was in the test chamber, orco mutants were just as attracted to the human odor treated stockings as the non–mutants were. B.The results suggest that CO2 could trigger host–seeking behavior even with nonfunctioning Orco–positive neurons. 4.A pro for the usage of 2,3–butanedione and pyridine is that they could inhibit cpA neuron or activate cpA neuron respectfully. A possible con could be the rancid butter smell of 2,3–butanedione. A definite con would be the high flammability and overall toxicity, even at low doses of pyridine. The fish–like odor could also be considered a con. The overall stopping of the mosquito bites (2,3–butanedione) and trapping of mosquitos (pyridine) is a ... Get more on HelpWriting.net ...
  • 32. How To Move Across A Semi-Permeable Cell Membrane The purpose of this lab is to determine which molecules can move across a semi–permeable membrane. Dialysis tubing, a thin membrane that behaves in much the same way as a living cell's actual plasma membrane, was used to test this experiment. After filling the dialysis tubing with the starch and glucose solutions, the artificial cell was then submerged in a water and iodine solution, and the results we're recorded after 30 minutes. The artificial cell had gained 1 gram of the water and iodine, but had not expelled any of it's starch and glucose solution. This experiment made it easy to see which molecules travelled and which were too big. Introduction Osmosis a process by which molecules pass through a semi–permeable membrane. This movement is described as movement from an area of less concentrated solution into a more concentrated one. The hypothesis being tested states that some molecules can pass through the membrane while other molecules cannot. If a molecule can move across the ... Show more content on Helpwriting.net ... The dialysis tubing, pre–soaked in water, was tied at one end to form a water tight seal. After opening the other end of the tube, 2 droppers of the starch solution and 2 droppers of the glucose solution were placed inside, before being tied off on the other end. The "cell" was weighed to determine its density before the experiment was conducted. Distilled water was measured out into a 150mL beaker and the "cell" is placed inside. 10 drops of iodine were added to the distilled water, and the contents of the beaker became visibly yellow. The beaker was moved aside and monitored for 30 minutes. After 30 minutes, the "cell" was removed from the liquid, dried with a paper towel, and weighed again. By weighing again, it was determined whether or not any substances had moved in or out of the artificial plasma ... Get more on HelpWriting.net ...
  • 33. Essay about Permeability of Beetroot Cell Membranes Permeability of Beetroot Cell Membranes An experiment to investigate how temperature affects the Permeability of beetroot cell membranes Interpretation of Results: 0В°C 20В°C 30В°C 40В°C 55В°C 0.24 0.28 0.75 0.79 0.22 0.26 0.35 0.41 0.81 From 0В°C to 30В°C there is a gradual increase of absorbency which shows that as the temperature increases it is denaturing the cell wall and cell membrane and allowing the beetroot pigment to leek out into the distilled water.
  • 34. As soon as the sample of beetroot is placed into the distilled water diffusion occurs naturally, which is the net movement of molecules from a region of high concentration (beetroot sample) to a region of low concentration (distilled water). From ... Show more content on Helpwriting.net ... Within my data which I collected I have found one anomaly. The anomaly is taken from my second row of results (highlighted in red). At the temperature 40В°C, the first result I collected from this temperature was 0.75 arbitrary units and from the re–test results at the same temperature I got 0.41 arbitrary units. This is a difference of 0.34 arbitrary units. This piece of data is not what I would have expected due to the first set of results, although the data at 55В°C on the first and second tests are relatively the same. Evaluation of Practical Work: The anomaly which I have identified at 40В°C is 0.41 arbitrary units this could be due to the size of the sample of beetroot used, if the sample was smaller than the other samples it was have less pigment which would suggested why the result is lower than expected. Another suggestion for why the result is lower than expected could be due to the loss of pigment in the preparation process, or from where the sample was taken from on the beetroot. If the sample was taken from the centre of the beetroot the sample may have had more pigment contained within the cells, but if the sample was taken from closer to the surface of the beetroot it ... Get more on HelpWriting.net ...
  • 35. Biology Exam Revision : Cell Membrane Biology Exam Revision Cells Cell MembraneThe cell membrane surrounds the cytoplasm of a cell, separating its contents from the environment around it. The cell surface membrane consists of two layers of phospholipids and regulates what can enter and leave the cell. It is a semi–permeable membrane embedded with proteins, including receptors on the outer surface. NucleusThe nucleus is the 'brain' of the cell. It is a large, round organelle and is surrounded by the nuclear envelope (a double membrane). The nuclear envelope allows the exchange of materials between the nucleus and cytoplasm through its large, nuclear pores. The nucleus contains DNA therefore the information to regulate the metabolism and reproduction for the cell. It also contains a dark staining area called the nucleolus. The nucleolus produces ribosomes. MitochondrionThe mitochondrion has a double membrane surrounding its ellipse shape. Some of the reactions of aerobic respiration (the process living things go through to use food energy) occur in the mitochondrion and they are the 'powerhouses' of the cell. They produce energy in the form of ATP. RibosomesRibosomes are the site of protein synthesis and are made up of two subunits. One of these units is large, and the other is small. These subunits consist of protein and ribosomal RNA. Ribosomes can be found floating free in a cell, or attached to the endoplasmic reticulum. Rough Endoplasmic ReticulumThe rough endoplasmic reticulum is covered with ... Get more on HelpWriting.net ...
  • 36. Cell Membrane Lab Report Cell membranes regulate the chemicals that go out or stay inside a cell. This lab tested these functions to see how the cell membrane regulates the chemicals that pass through it. The membrane tries to achieve equilibrium on both sides by sending some chemicals to the outside or inside to get the same amount of each chemical. Dialysis tubing was used as a cell membrane. The cell membrane was simulated by dialysis tubing. The tubing was folded, wetted and tied to allow it to look like a cell. It was then filled в…” by starch solution, tied shut and wetted from the outside. It was then placed into a beaker with ВЅ water and enough iodine to give the water a yellow–type colour. Observations of the tubing were recorded at the initial stage, after 15 minutes and after a 48 hour period. ... Show more content on Helpwriting.net ... It was very easy to see that there was no colour inside the tubing but outside, it was a yellow–type colour due to the water and iodine solution. After 15 minutes, the colour of the contents in the tubing had changed. It was now a navy blue and black colour. However, this colour was mainly in the top area of the tube (closer to the surface of the water). The solution in the beaker had also changed colour. The bolder yellow colour had changed into a much lighter yellow. After 48 hours, the inside of the tubing had completely changed colour as the entire thing was a very navy blue and black. The contents of the beaker had turned into a very clear substance with a yellowish colour on the bottom of the beaker. There was also a lot of bubbles on the bottom, where the yellowish colour was, as well as near the middle of the ... Get more on HelpWriting.net ...
  • 37. Cell Membrane Structure 1 – Describe the normal structure and functioning of cell membranes, and explain how they compare to the membranes relating to a specific abnormality in cystic fibrosis? All cells have a cell membrane. The structure of membranes is formed from a double layer of phospholipids with proteins floating in it. The proteins are embedded on the surface and inside or bridge the double layers of phospholipids. This structure is called a mosaic model. The main function of cell membranes is to provide protection and support for the cell and they also control what enter and exit the cell to maintain internal balance, called homeostasis. There are two types of a membrane protein: integral proteins and peripheral proteins. The integral membrane proteins are ... Show more content on Helpwriting.net ... The cystic fibrosis affects the exocrine gland, which are organs that secrete mucus. It normally produces thin, slippery secretions such as mucus, sweat, tears and digestive juice. The organs can be affected by cystic fibrosis are sweat glands, respiratory system, digestive system and reproductive system. Sweat glands: The individual who suffers from cystic fibrosis, the amount of body salts lost in the sweat are higher than the normal people. This can cause problems during times of increased sweating. Such as hot weather. Respiratory system: The lining of the respiratory tract which made of tiny, hair– like cells called the cilia. Their function is to keep the lung clean and clear from foreign substances and mucus. The person who suffers from cystic fibrosis, the cilia can not easily remove the thick and sticky mucus out of the air passages in the lung. The mucus clogs the air passages, causing a chronic cough and lung infections. Repeated of lung infections can lead to damage to the lung and leading to not get enough oxygen into the body. Digestive system: The thick and sticky mucus blocks the pancreatic ducts and preventing the enzymes from reaching in the small intestine to digest food. The result is incomplete digestion and poor absorption of food ... Get more on HelpWriting.net ...
  • 38. Semipermeability Of The Cell Membranes A membrane is the cell's gatekeeper. This phospholipid bilayer says what molecules can move in or out of the cell. The cell membrane is also responsible for maintaining homeostasis. The cell membrane is semipermeable. They can control what molecules come in or out. There are two ways that molecules can move across the membrane. Passive mechanisms like diffusion dont use any energy while active transport uses energy to move molecules. Diffusion is the movement of particles down their gradient. Simple Diffusion is the passive movement from high to low concentration. Facilitated Diffusion is the movement of particles from high concentration to low concentration through a protein carrier. Osmosis is the diffusion of water across the cell membrane. steepness of gradient, temperature, size of molecule, and distance are all factors in the rate of diffusion. Tonicity refers to the concentration of solute in the solvent. A typical solution consists of two components known as the solvent and the solute. The solvent is used as a dissolving medium while the solute is used as the substance dissolved in the solvent. In a hypotonic solution, there is a lower concentration of solute relative to the inside of the cell. In a hypertonic solution, there is a high concentration of solute relative to the inside of the cell. In plants cells, the swelling of cells placed in a hypotonic solution results in turgor pressure. The structure of the cell stops it from bursting. Turgor pressure keeps the ... Get more on HelpWriting.net ...
  • 39. Investigation Of The Cell Membrane Essay Mustafa Elgabry October 17, 2016 Period 3 Egg Lab Introduction: The cell membrane benifits the cell in many different ways and is composed of many different parts which help the membrane execute what it is supposed to practice. A model that represents these parts is called the fluid mosaic model. The model contains proteins and carbohydrates within a phospholipid bilayer that consists of hydrophobic tails and hydrophilic heads. All of the components within the bilayer play a role in the function of the cell membrane. For example the carbohydrates practice cell to cell recognition while the proteins transport molecules in the cell and back out. Proteins also have alternate functions such as intercellular joining and extracellular matrix. When molecules transport in and out of the cell through the membrane, there are two types of transport, passive and active transports. Passive transport occurs when molecules move with the concentration gradient. One example is simple diffusion, where the molecules transport through the cell membrane effortlessly moving from a high to low concentration. Then there is facilitated diffusion where the molecules such as glucose move with the concentration gradient but have to use a protein to pass through the membrane. Osmosis, however, is the diffusion of water molecules across the membrane, but the water molecules also need to pass through a protein because the inside of the lipid bilayer consists of hydrophobic tails. The purpose ... Get more on HelpWriting.net ...
  • 40. The Role Of Proteins Of Cell Membrane Transport Discuss the role of proteins in cell membrane transport essay plan Cell membranes are surrounded by a phospholipid bilayer that provides a semipermeable barrier for cells, separating the cytosol from the extracellular environment. Phospholipids are ampithatic, meaning that they have a hydrophilic head and hydrophobic tail, which causes the heads to face outwards towards the water and the tails inwards, creating the bilayer [figure 1]. Small hydrophobic molecules such as O2 and CO2 and small uncharged polar molecules such as H2O and ethanol can diffuse through this bilayer, however larger molecules and ions cannot, and thus require proteins, which are polymers of amino acids joined together by strong peptide bonds. These proteins feature throughout the membrane, and account for around 50% of its mass [http://www.ncbi.nlm.nih.gov/books/NBK9898/] . Not only are proteins required for transport of molecules through the membrane, but they also transport signals and are necessary for the cell support; throughout this essay I will focus on the pivotal role they play with regards to the transport of these molecules and signals, and what occurs when these functions are inhibited. There are two main types of membrane transport proteins: firstly, there are channel proteins, which are essentially pores that extend across the membrane. These pores create a hydrophilic passage for molecules through he hydrophobic bilayer. The rate of movement through these pores is significantly faster ... Get more on HelpWriting.net ...
  • 41. Cell Membrane Diffusion Question– How does changing a solution's concentration of solutes affect the movement of molecules across a cell membrane? Background Research– The cell membrane's function is to control what goes in and out of the cell. The membrane isolates the area of cytoplasm from the external environment and regulates the exchange of entering and exiting molecules. Cell membranes communicate with other organelles to function properly.(Muskopf). A quality of the membrane is that it is selectively permeable. This means that certain substances may pass through the membrane more easily than others. The cell membrane consists of a phospholipid bilayer and embedded proteins.(Feldkamp, page 73). Another characteristic of the cell membrane is the idea of passive transport. Passive transport is when certain substances inside of the cell can move throughout the membrane without the use of the cells ... Show more content on Helpwriting.net ... The way that a molecule can move from an area of a high to low concentration is simple diffusion. Concentration gradient is the term referring to the difference between the concentration levels. For example, when a sugar cube enters into a water beaker, the sugar sinks to the bottom and the level of concentration increases. The concentration of the sugar molecules goes up compared to the level at the top of the water. Eventually, the sugar cube will dissolve and the molecules diffuse, those molecules disperse throughout the water beaker.(Feldkamp, page 95). A different type of diffusion is facilitated diffusion. This is the process in which molecules can not dissolve at a moderate rate throughout the area of the membrane. An example of facilitated diffusion are carrier proteins. Certain molecules are not able to dissolve. Carrier proteins assist the movement of these molecules across the membrane. These proteins move the molecules from areas of high to low concentration without the use of energy.(Feldkamp, page ... Get more on HelpWriting.net ...
  • 42. Biology : Cell Membrane Transport Cell Membrane Transport Ard Orpilla Zoology 2011 Section 16 Manisha Rao October 6, 2014 Introduction Cells are the basic units of life. Everything alive consists of cells. In order for cells to perform their basic functions, it has to continuously seek homeostasis. This is done by structures within the cell. A structure within the cell is the plasma membrane, which regulates what enters and exits the cell. The cell membrane contains a phospholipid bilayer which causes the cell to have selective permeability; "allowing transport of some chemicals but not others" ( ). Specifically, the phospholipid bilayer allows for the movement of water, oxygen, and carbon dioxide. Conversely, it is not permeable to ions or polar chemicals. The phospholipid bilayer is in possession of hydrophilic and hydrophobic regions, which are the polar heads and tails, respectively. This is important because the head attracts water while the tail repels water, thus regulating the substances entering and exiting the cell. When substances pass through the plasma membrane, they use passive or active transport. Whether either transport type is used is determined by the concentration gradient, which is the difference of concentration between areas. In an active transport, the cell must use adenosine triphosphate, or ATP, which is produced by the mitochondria of the cell and is energy used by the body, because the substance is moving from a lower concentration gradient to a higher ... Get more on HelpWriting.net ...
  • 43. The Effects Of Environmental Stress On Cell Membranes Introduction: The purpose of this experiment was to indirectly study the effects of environmental stress on cell membranes. Watercourses have been with discarding of domestic and manufacturing, for centuries. Alarms have only escalated about the ecological alterations in the recent years of what would be the consequences. Scientists have developed a thorough investigation in search of various man harmful waste that is damaging the environment of watercourses. The cell membranes are the utmost essential organelle that surrounds all living cells. Its purpose is to control what goes in and out of the cells and is accountable for the various other properties of the cells as well. The nucleus and other organelles also have membranes that are practically indistinguishable. Membranes are organised in a mosaic arrangement, comprised of carbohydrates, proteins and phospholipids. This can be seen in Figure 1. The objective of this indirect examination is to study the causes of various solvents and conducts on live beetroot cells. The reason why beetroot cells have been selected for this experiment is because they have a big membrane–bound central vacuole, as seen in Figure 2. The red colour anthocyanin, which provides the beetroot its bright colour is located in the vacuole. The cell membrane encloses the whole beetroot cell. The anthocyanin cannot leak out if the membranes stay unharmed. The red colour can escape if the membranes are hassled or broken. Abstract: Ethanol disrupts ... Get more on HelpWriting.net ...
  • 44. HM Complexation At The Cell Wall Plasma Membrane Interface... 3 HM complexation at the cell wall–plasma membrane interface The cell wall plasma membrane (CWPs) interface is believed as a key site for the HM stress signal perception. The CWPs involved in response to various abiotic stresses, have been extensively identified and characterised in different crop plants. Under various stress conditions, the main CWPs include phospholipases, the salt overly sensitive kinases, transcription factors, C–repeat binding factor, dehydration sensitive element binding proteins, mitogen activated proteins kinases and phosphatases, and abscisic acid responsive element binding factors (Ihsan et al., 2017; Dar et al., 2017). There are known CW proteins which forms covalent bonds and undergo glycosylation with HMs some ... Show more content on Helpwriting.net ... Various plantcell organelles are involved in metal ions' sequestration but the homeostatic role of vacuole in conferring HM stress tolerance is well established (Bashir et al., 2016). Therefore, the central vacuole seems to be a suitable storage bucket/reservoir for excessively accumulated metal pollutants. In fact, two vacuolar proton pumps, a vacuolar proton–ATPase (VATPase) and vacuolar proton pyrophosphates (VPPase),accelerate vacuolar uptake of most solutes (Yan et al., 2010), which is catalyzed by either channels or transporters (Yasir et al., 2011).Few well –characterized HM transporter proteins include zinc–regulated transporter (ZRT), iron–regulated transporter (IRT) like protein ZIP family, ATP –binding cassette (ABC) transporters, the P–type metal ATPases, the natural resistance–associated macrophage protein (NRAMP) family, multidrug resistance–associated proteins (MRP), ABC transporters of the mitochondria (ATM), cation diffusion facilitator (CDF) family of proteins, copper transporter (COPT) family proteins, pleiotropic drug resistance (PDR) transporters, yellow–stripe–like (YSL) transporter and Ca2+ cation antiporters (CAX)(Dimkpa et al., 2015; Taiz and Zeigher 2010). Evidence for plant vacuoles as the main reservoirs ... Get more on HelpWriting.net ...
  • 45. Investigate the Effect of Temperature on Cell Membranes... Investigate the Effect of Temperature on Cell Membranes and Membrane Structure If you read a recipe for cooked beetroot it will usually recommend that you don't remove the outer skin of the beetroot and don't cut off al the stalk and root if you want to avoid getting lots of red dye in the cooking water. Beetroot contains red pigments called betalains, located within the cell vacuole. Normally the pigments can't pass through membranes but they leak out when the beetroot is cooked. Aim – The aim of this practical is to use beetroot to examine the effect of the temperature on cell membranes and relate the effects observed to membrane structure. To function correctly a cell needs to be able to control transport across the... Show more content on Helpwriting.net ... The polar heads lie at both surfaces, the kind of three–dimensional enclosed structure shown above can form. The real shape tends to be globular (minimizing surface to volume ratio), and there are so many more bipolar molecule pairs than shown here that the radius of curvature lacks the kind of strain and distortion shown at the corners of this drawing. A membrane is a phospholipid bilayer studded with proteins, polysaccharides and other sorts of lipids. This patchwork of molecules is like a mosaic. Because the molecules move about within their respective layer, the membrane behaves like a fluid. This is why the model is called the Fluid–mosaic model. There have been many theories as to the nature of the cell membrane. In 1935 Daniellie and Davson put forward a possible structure, which was widely accepted. Their model was composed of a double lipid layer coated on each side by protein. They Estimated that the membrane was so thick, with the introduction of the electron microscope, membranes could be clearly seen for the first time. Plasma membranes of both animal and plant cells showed a characteristic three–layered (trilarninar) appearance. As we apply heat on to the beetroot, this denatures the proteins creating "holes" in the membrane allowing pigment to be leeched out. The denaturing of the proteins incorporated with the added kinetic energy added to the polysaccharides allows pigment to be
  • 46. ... Get more on HelpWriting.net ...
  • 47. The Effect Of Temperature On The Extent Of Cell Membrane... Purpose: To determine the effects of temperature stress on the extent of cell membrane damage. To determine the damaging effects Acetone and Ethanol concentration on cell membrane damage. Introduction: The cell membrane (also known as the plasma membrane) is a "thin semi–permeable membrane that surrounds the cytoplasm of a cell" (Bailey, 2010). The purpose of cell membrane is to serve as a barrier or gate keeper. Some molecules are able to diffuse across the lipid bilayer while others are prohibited from entering the cell. (The Editors of EncyclopГ¦dia Britannica, 2016). Allowing only specific molecules to enter the cell and keeping others out of the cell is what gives the cell membrane the "semipermeable" characteristic (Bailey, ... Show more content on Helpwriting.net ... This specific structure gives reason as to why temperature, acetone and ethanol affect the membrane. Temperature helps to "determine what can enter or leave the cell and how well molecules found within the membrane can function" (Chandler, 2017). Cells function best at a temperature that corresponds to the physiological temperature, which is 98.6В°C. An increase of temperature increases fluidity in the cell membrane. This is a result of the fatty acid tails becoming less rigid and allowing for more movement. It leads to a change in the permeability of the cell allowing some possibly harmful molecules in. For temperature decrease, the cell becomes less fluid and more rigid. Also affecting the permeability of the membrane, restricting possibly important molecules from entering (Chandler, 2017). Acetone is known as an organicsolvent which causes the lipid components of the cell membrane to dissolve leading to the breakdown of the cell membrane (Shukla, 2016). With a higher acetone concentration, results in the increase of membrane fluidity. This is due to the "acetone molecules pushing the lipid heads apart" (Posokhov, Kyrychenko, 2013). Similarly, ethanol is also an organic solvent. The cell membrane can not hold its shape in the presence of ethanol leading to the membrane becoming more fluid. The cell membrane is then more permeable because of ethanol molecules penetrating the bilayer. This also has a direct ... Get more on HelpWriting.net ...