Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: Enzymes are proteins.) If necessary, it may be useful for you to review the Scientific Method Tutorial (found in the Course Content section of the classroom). Lab Materials You may need the following, depending on your experimental design: Materials in your lab kit: none Additional materials you may need: plastic beakers or cups hydrogen peroxide solution (from Lab 4) yeast (from Lab 4) sample of fresh meat, about 1 cm cubed in size (unprocessed and uncooked, e.g., liver, steak, fish, or poultry—particularly organs) sample of fresh vegetable, about 1 cm cubed in size (e.g., potatoes or other root vegetables) a pen for labeling the beakers or cups a ruler with centimeter markings 1. Write a brief introduction. Include the question that you are asking and a clear hypothesis for your experiment (15 points). 2. Design an experiment. Provide a detailed account of the materials and methods used to conduct the experiment. Also include the methods for data collection and analysis (20 points). 3. Conduct the experiment and record your results below. What did you observe? Which samples showed bubbling? Rate the bubbling for comparison between samples. Present your data in table or graph format. You might also include pictures (20 points). 4. Use your knowledge about enzymes to interpret and discuss your results. It may be necessary for you to refer to your textbook and/or use other information resources. What effect does the acid have on the enzyme? Looking back, how could you have improved the experiment? (20 points) 5. What is your conclusion? Was your hypothesis supported? (15 points) 6. Cite all reference sources used (including text book) and provide a reference section with citations in APA format (10 points). Grading Your Final Applied Lab Project will be graded based on the following criteria (100 points total): Excellent Good Poor Unacceptable Outline 5 points Outline includes brief description of planned experiment that meets the assignment description. Submitted on time. 5 points Outline includes brief description of planned experiment, but a few issues with content, relevance and/or organization. Submitted on time. 3-4 points Outline includes brief description of planned experiment, but major issues with content, relevance and/or organization, and/or not submitted on time. 1-2 points No outline submitted 0 points Introduction, Question and Hypothesis 15 points Accurate and specific introduction, question and hypothesis that is directly related to the experiment 12-15 points Introduction, question, and/or hypothesis is somewhat vague or includes some mitakes, and/or not directly related to experiment 5-11 points Missing introduction, question and/or hypothesis, and/or very vague and/or not related to experiment. 1-.

1. Design an experiment in which you will test the effect of an
acidic fluid on enzymatic activity. (Recall: Enzymes are
proteins.) If necessary, it may be useful for you to review the
Scientific Method Tutorial (found in the Course Content section
of the classroom).
Lab Materials
You may need the following, depending on your experimental
design:
Materials in your lab kit:
none
Additional materials you may need:
plastic beakers or cups
hydrogen peroxide solution (from Lab 4)
yeast (from Lab 4)
sample of fresh meat, about 1 cm cubed in size (unprocessed
and uncooked, e.g., liver, steak, fish, or poultry—particularly

2. organs)
sample of fresh vegetable, about 1 cm cubed in size (e.g.,
potatoes or other root vegetables)
a pen for labeling the beakers or cups
a ruler with centimeter markings
1. Write a brief introduction. Include the question that you are
asking and a clear hypothesis for your experiment (15 points).
2. Design an experiment. Provide a detailed account of the
materials and methods used to conduct the experiment. Also
include the methods for data collection and analysis (20 points).
3. Conduct the experiment and record your results below. What
did you observe? Which samples showed bubbling? Rate the
bubbling for comparison between samples. Present your data in
table or graph format. You might also include pictures (20
points).
4. Use your knowledge about enzymes to interpret and discuss
your results. It may be necessary for you to refer to your
textbook and/or use other information resources. What effect
does the acid have on the enzyme? Looking back, how could

3. you have improved the experiment? (20 points)
5. What is your conclusion? Was your hypothesis supported?
(15 points)
6. Cite all reference sources used (including text book) and
provide a reference section with citations in APA format (10
points).
Grading
Your Final Applied Lab Project will be graded based on the
following criteria (100 points total):
Excellent
Good
Poor
Unacceptable

4. Outline
5 points
Outline includes brief description of planned experiment that
meets the assignment description. Submitted on time.
5 points
Outline includes brief description of planned experiment, but a
few issues with content, relevance and/or organization.
Submitted on time.
3-4 points
Outline includes brief description of planned experiment, but
major issues with content, relevance and/or organization, and/or
not submitted on time.
1-2 points
No outline submitted
0 points

5. Introduction, Question and Hypothesis
15 points
Accurate and specific introduction, question and hypothesis
that is directly related to the experiment
12-15 points
Introduction, question, and/or hypothesis is somewhat vague or
includes some mitakes, and/or not directly related to experiment
5-11 points
Missing introduction, question and/or hypothesis, and/or very
vague and/or not related to experiment.
1-4 point
Question and hypothesis missing

6. 0 points
Experimental Design, Materials and Methods
20 points
Experiment is designed to directly test the hypothesis;
description of experiment is detailed and well written and
includes all materials and methods used.
18-20 points
Experiment is designed to directly test the hypothesis, but
description is somewhat inaccurate and/or some information
about materials and methods used is missing and/or minor
problems with language and/or organization.

7. 9-17 points
Experiment is not well designed to test the hypothesis, and/or
most of the materials and methods used are not described well,
and/or major problems with language and organization.
1-8 points
No description of experimental design, materials and methods
used.
0 points
Results
20 points
Results are clearly and accurately presented in a table or graph
format. Pictures support the data in the table and/or graphs.
18-20 points

8. Results are presented, but minor problems with clarity and/or
accuracy.
9-17 points
Results are described, but major problems with clarity and/or
accuracy and/or results not presented in table or graph.
1-8 points
Results are not included.
0 points
Discussion of Results
20 points

9. A well written discussion of results that includes knowledge
about enzymes learned from text book and course modules
and/or web-sources and how experiment could have been
improved.
18-20 points
Discussion of results and how experiment could have been
improved has some minor problems with accuracy and/or
organization and/or missing some information and/or not well
connected to knowledge about enzymes learned from text
book/course modules and/or web sources.
9-17 points
Discussion of results and how experiment could have been
improved has some major problems with accuracy and/or
organization and/or missing important information and/or not
connected to knowledge about enzymes learned from text
book/course modules and/or web-sources.
1-8 points
No discussion of results and no connection to knowledge about
enzymes from text book and course modules.

10. 0 points
Conclusion
10 points
A well stated conclusion that is accurate and specific and
directly related to the data collected. Clearly stated whether or
not the conclusion supports the hypothesis.
9-10 points

11. Conclusion stated, but slightly inaccurate and/or missing
specific information, and/or not directly related to data
collected.
4-8 points
Conclusion is vague, inaccurate and/or not directly related to
the experiment and/or missing statement about whether or not
conclusion is supported by hypothesis.
1-3 point
Missing conclusion

12. 0 points
Citations
5 points
All references used are cited in correct APA format, in-text
references are included.
5 points
All references are included, but a few minor mistakes in APA
format, and/or missing in-text references.
3-4 points
Missing one or several references and/or several mistakes in
APA format

13. 1-2 point
No references are included
0 points
Grammar and Spelling
5 points
Less than 5 minor spelling and grammatical errors
5 points
Between 5 and 10 minor spelling and grammatical errors

14. 3 points
Between 10 and 20 spelling and grammatical errors
1 point
More than 20 spelling and grammatical errors
0 point