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Final Applied Lab Project-BIOL 103The Effect of low pH on
Enzyme Activity
Design an experiment in which you will test the effect of an
acidic fluid on enzymatic activity. Recall: enzymes are proteins!
To complete this project, it may be useful for you to first
review the Scientific Method Tutorial, the OLI module about pH
and enzymes, Lab 1 (Introduction to Science) and Lab 4
(Enzymes). As you review Lab 4, you will be reminded that
there are several factors that impact enzymatic activity: pH,
temperature, and amount of reagent. It is OK to use the same
enzyme/substrate/method as you did in lab 4 (but modify the
treatment), but you are encouraged to search on-line to find a
different enzyme/substrate/method for measuring enzyme
activity for your project (include all references).
As you design your experiment for this project, please
remember that you are trying to examine how an acidic fluid
will modify the outcome of an enzymatic reaction. To
successfully complete this project, you will need to identify the
question(s) being asked in your experiment and the hypothesis
that you are testing. In your experimental design, you must
clearly explain what you are doing. That means that you will
need to identify the enzyme, the substrate, the acidic fluid used
as treatment, the control treatment and the method of measuring
enzyme activity, as well as explain your experimental protocol.
You must also thoroughly explain how the acidic fluid impacted
enzyme activity based on the results from your own experiment
as well as knowledge of enzymes and pH from the OLI modules,
lab manual and potentially additional information sources.
Submit Files - Final Applied Lab Project
Top of Form
Folder
Final Applied Lab Project
Instructions
Final Applied Lab Project
Addresses course outcomes 1-4:
· recognize and explain how the scientific method is used to
solve problems
· make observations and discriminate between scientific and
pseudoscientific explanations
· weigh evidence and make decisions based on strengths and
limitations of scientific knowledge and the scientific method
· use knowledge of biological principles, the scientific method,
and appropriate technologies to ask relevant questions, develop
hypotheses, design and conduct experiments, interpret results,
and draw conclusions
This is the culminating lab assessment in BIOL 103. It is
designed to assess your ability to apply the principles of the
scientific method.
The Effect of low pH on Enzyme Activity
Design an experiment in which you will test the effect of an
acidic fluid on enzymatic activity. Recall: enzymes are proteins!
To complete this project, it may be useful for you to first
review the Scientific Method Tutorial, the OLI module about pH
and enzymes, Lab 1 (Introduction to Science) and Lab 4
(Enzymes). As you review Lab 4, you will be reminded that
there are several factors that impact enzymatic activity: pH,
temperature, and amount of reagent. It is OK to use the same
enzyme/substrate/method as you did in lab 4 (but modify the
treatment), but you are encouraged to search on-line to find a
different enzyme/substrate/method for measuring enzyme
activity for your project (include all references).
As you design your experiment for this project, please
remember that you are trying to examine how an acidic fluid
will modify the outcome of an enzymatic reaction. To
successfully complete this project, you will need to identify the
question(s) being asked in your experiment and the hypothesis
that you are testing. In your experimental design, you must
clearly explain what you are doing. That means that you will
need to identify the enzyme, the substrate, the acidic fluid used
as treatment, the control treatment and the method of measuring
enzyme activity, as well as explain your experimental protocol.
You must also thoroughly explain how the acidic fluid impacted
enzyme activity based on the results from your own experiment
as well as knowledge of enzymes and pH from the OLI modules,
lab manual and potentially additional information sources.
Hint: Keep in mind that the acid will change the environmental
conditions of the experiment (for example, a low pH value
could change the shape of the active site on the enzyme
protein), without directly participating in the reaction.
Lab Materials
You may need all or some of the following, depending on your
experimental design:
Materials from your lab kit:
· pH paper
· hydrogen peroxide solution (you can purchase this at a
pharmacy if you have used up the bottle that came with the lab
kit)
· plastic beakers or cups
· vinegar
· yeast (can be purchased at grocery store if more is needed)
· balloons
· plastic bottle
· marker for labeling of beakers
You may choose to use additional materials (different acidic
fluids and/or different organisms and/or different substrate if
you chose an enzyme other than catalase).
Outline (10 points) submit in assignment folder in week 7.
· Name of enzyme you will use: Yeast
· Name of organism (if applicable): N/A
· The substrate: Hydrogen peroxide
· Method for measuring enzyme activity: Circumference of
balloons
· Treatment: acidic fluid(s), pH, length of exposure, how you
will treat your samples: length of exposure will be 1min, 3min,
5min
· The control(s) in the experiment: 3 Tubes filled with 10ml of
catalase, 10ml of hydrogen peroxide and no acetic acid
· Sample size: 3 tubes with 10ml of catalase, 10ml of hydrogen
peroxide and each one with 1ml, 3ml, 5ml of acetic acid
· Hypothesis: When acetic fluids are added to enzyme-substrate
mixtures, enzyme activity is affected.
· How you will present your data (table and/or type of graph):
Table
Write a lab report that includes the following:
1. Title page: descriptive title, your name, course name,
semester
2. Introduction: general background information about enzymes
and specific information about your chosen enzyme, the
question(s) that you are asking and a clear hypothesis for your
experiment (15 points).
3. Design an experiment. Provide a detailed description of the
materials and methods used to conduct the experiment. Identify
control and experimental samples, as well as independent and
dependent variables. Also include the methods used for data
collection and analysis (20 points).
4. Conduct the experiment and record your results. What did
you observe? Present your data in table and/or graph format.
Remember to label everything and include the unit of measure
with all numbers (20 points)
5. Use your knowledge of enzymes and pH to interpret and
discuss your results. It may be necessary for you to refer to the
OLI course modules, lab manual and/or use additional
information resources. What effect does the acidic treatment
have on enzyme activity? Did you get the expected results?
Explain. (15 points)
6. State a specific and accurate conclusion. Is your hypothesis
supported by the results? Looking back, how could you have
improved your experiment? (10 points)
7. Include a list of references to all information sources used in
APA format (5 points).
Avoid Plagiarism
It is very important to write with your own words. If you do
copy one or two sentences directly (use sparingly), use
quotation marks (") around the copied text, followed by an in-
text reference. All information sources need to be included in
the reference list and as in-text references. Plagiarism will be
reported to the proper UMUC authority. Guidance on how to
avoid plagiarism can be found here:
Submission
Submit your final applied lab project to both the TurnItIn
website as well as in the assignment folder by the due date
specified in the course schedule.
Grading
Your Final Applied Lab Project will be graded based on the
following criteria (100 points total):
Rubric Name: Final Applied Laboratory Project
Criteria
Excellent (90-100%)
100 points
Good (75-89%)
89 points
Adequate (50-74%)
74 points
Unacceptable (0-49%)
49 points
Outline 10 points (Due in week 7)
Outline includes all the required components and planned
experiment meets requirements for the assignment and is clearly
and accurately described. Submitted on time.
9-10 points
Outline missing one or two of the required components, and/or
planned experiment does not meet one of requirements for the
assignment and/or minor issues with clarity and accuracy.
7-8 points
Outline missing several of the required components, and/or
planned experiment does not meet several of the requirements
for the assignment and/or major issues with clarity and
accuracy.
5-6 points
Outline missing most of the required components andplanned
experiment does not meet the requirements of the assignment.
0-4 points
Introduction, Question and Hypothesis (15 points)
Background information about enzymes in general and about
specific enzyme used in project is clearly and accurately
written. Questions and hypothesis are specific, relevant and
clearly stated.
14-15 points
Background information about enzymes in general and about
specific enzyme is somewhat unclear and/or inaccurate.
Questions and hypothesis could be more specific, relevant and
clearly stated.
11-13 points
Missing background information about enzymes in general or
about specific enzyme used in project or questions and
hypothesis.
7-12 point
Missing background information about enzymes in general and
about specific enzyme used in project andquestions/hypothesis.
0-6 points
Experimental Design, Materials and Methods (20 points)
Experiment is designed to directly test the hypothesis;
description of experiment is detailed and well written and
includes all materials and methods used.
18-20 points
Experiment is mostly designed to test the hypothesis and/or
description of experiment is somewhat inaccurate and/or some
information about materials and methods used is missing and/or
minor problems with clarity an organization.
15-17 points
Experiment is barely designed to test the hypothesis and/or
description of experiment is inaccurate and/or most of the
materials and methods used are missing and/or major problems
with clarity an organization.
10-14 points
Experiment is not designed to test hypothesis and/or description
of experiment and materials and methods used are missing.
0-9 points
Results (20 points)
Results are clearly and accurately presented in a table and/or
graph format.
18-20 points
Results are presented, but minor problems with clarity and/or
accuracy.
15-17 points
Results are described, but major problems with clarity and/or
accuracy and/or results not presented in table or graph.
10-14 points
Results are not included
0-9 points
Discussion of Results (15 points)
A clear, accurate and well organized discussion of results that
demonstrates good knowledge of enzymes.
14-15 points
Minor problems with clarity, accuracy or organization and/or
demonstrates some gaps in knowledge of enzymes.
15-17 points
Major problems with clarity, accuracy or organization or
demonstrates minimal knowledge of enzymes.
10-14 points
Missing discussion of results, or major problems with clarity,
accuracy or organization andmissing understanding of enzymes.
0-9 points
Conclusion ( 10 points )
A well stated conclusion that is accurate and specific and
directly related to the data collected. Clearly stated whether or
not the results support the hypothesis. Relevant suggestion for
improvement of experiment.
9-10 points
Conclusion stated, but slightly inaccurate, and/or not directly
related to data collected and/or suggestion for improvement of
experiment not very relevant.
7-8 points
Conclusion stated, but inaccurate and/or barely related to data
collected and/or missing suggestion for improvement of
experiment.
5-6 points
Missing conclusion, only suggestion for improvement of
experiment.
0-4 points
Citations (5 points)
All references used are included and in correct APA format, in-
text references are included.
5 points
All references are included, but a few minor mistakes in APA
format, and/or missing in-text references.
4 points
Missing one or several references and/or major mistakes in APA
format.
3 point
No references included or references not in APA format.
0-2 points
Grammar and Spelling (5 points)
Less than 3 minor spelling and grammatical errors
5 points
Between 4 and 7 minor spelling and grammatical errors
4 points
Between 8 and 12 spelling and grammatical errors
3 point
More than 13 spelling and grammatical errors
0-2 points
Overall Score
Level 4
90 or more
Level 3
75 or more
Level 2
50 or more
Level 1
0 or more
Bottom of Form
Final Project Outline
The Effect of low pH on Enzyme Activity in Raw Meat
Purpose: To recognize the impact of acid on based on the
activity of enzymes
Hypothesis: When acetic fluids are added to enzyme-
substrate mixtures, enzyme activity is affected.
Experiment: Observation of the yeast and temperature
regulated hydrogen peroxide in the presence of food.
Materials:
· Name of enzyme you will use: Yeast
· Name of organism (if applicable): Liver, carrot
· The substrate: Hydrogen peroxide
· Method for measuring enzyme activity: Circumference of
balloons
· Treatment: acidic fluid(s), pH, length of exposure, how you
will treat your samples: length of exposure will be 1 min, 3
min, 5 min
· The control(s) in the experiment: 3 Tubes filled with 10ml of
catalase, 10ml of hydrogen peroxide and no acetic acid
· Sample size: 3 tubes with 10ml of catalase, 10ml of hydrogen
peroxide and each one with 1ml, 3ml, 5ml of acetic acid
· Hypothesis: When acetic fluids are added to enzyme-substrate
mixtures, enzyme activity is affected.
· How you will present your data (table and/or type of graph):
Table
Procedure: 1. Cut 1 cm of meet and add to 200 ml
cylinder
2. Add yeast into the same 200 ml cylinder
3. Add Hydrogen peroxide into the testing
tube.
4. Cylinder and testing tube is kept in water
both temperatures 80 degrees F.
5. Pour hot Hydrogen Peroxide in the cylinder
that contains meat and yeast.
6. Volume of both cylinder and testing tube
will be measured, before and after
combining all items.
7. After 3 minutes the volume is measured
again and recorded
8. Initial volume will be subtracted from final
volume, to obtain the actual amount of
foam.
9. Repeat by replace the acidic food with the
alkaline one.
10. The data obtained will be used to create the
table.
Data and Analysis: observing the altered pH condition and the
level of oxygen that is generated will collect
Data
Conclusion: the results of my exeriment
References: Any references used (i.e.: internet, books,
documentation)
Final Applied Lab Project-BIOL 103The Effect of low pH on Enzyme A.docx

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Final Applied Lab Project-BIOL 103The Effect of low pH on Enzyme A.docx

  • 1. Final Applied Lab Project-BIOL 103The Effect of low pH on Enzyme Activity Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, the OLI module about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), but you are encouraged to search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references). As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the OLI modules, lab manual and potentially additional information sources. Submit Files - Final Applied Lab Project Top of Form
  • 2. Folder Final Applied Lab Project Instructions Final Applied Lab Project Addresses course outcomes 1-4: · recognize and explain how the scientific method is used to solve problems · make observations and discriminate between scientific and pseudoscientific explanations · weigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific method · use knowledge of biological principles, the scientific method, and appropriate technologies to ask relevant questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusions This is the culminating lab assessment in BIOL 103. It is designed to assess your ability to apply the principles of the scientific method. The Effect of low pH on Enzyme Activity Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, the OLI module about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that
  • 3. there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), but you are encouraged to search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references). As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the OLI modules, lab manual and potentially additional information sources. Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH value could change the shape of the active site on the enzyme protein), without directly participating in the reaction. Lab Materials You may need all or some of the following, depending on your experimental design: Materials from your lab kit: · pH paper · hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit)
  • 4. · plastic beakers or cups · vinegar · yeast (can be purchased at grocery store if more is needed) · balloons · plastic bottle · marker for labeling of beakers You may choose to use additional materials (different acidic fluids and/or different organisms and/or different substrate if you chose an enzyme other than catalase). Outline (10 points) submit in assignment folder in week 7. · Name of enzyme you will use: Yeast · Name of organism (if applicable): N/A · The substrate: Hydrogen peroxide · Method for measuring enzyme activity: Circumference of balloons · Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples: length of exposure will be 1min, 3min, 5min · The control(s) in the experiment: 3 Tubes filled with 10ml of catalase, 10ml of hydrogen peroxide and no acetic acid · Sample size: 3 tubes with 10ml of catalase, 10ml of hydrogen peroxide and each one with 1ml, 3ml, 5ml of acetic acid
  • 5. · Hypothesis: When acetic fluids are added to enzyme-substrate mixtures, enzyme activity is affected. · How you will present your data (table and/or type of graph): Table Write a lab report that includes the following: 1. Title page: descriptive title, your name, course name, semester 2. Introduction: general background information about enzymes and specific information about your chosen enzyme, the question(s) that you are asking and a clear hypothesis for your experiment (15 points). 3. Design an experiment. Provide a detailed description of the materials and methods used to conduct the experiment. Identify control and experimental samples, as well as independent and dependent variables. Also include the methods used for data collection and analysis (20 points). 4. Conduct the experiment and record your results. What did you observe? Present your data in table and/or graph format. Remember to label everything and include the unit of measure with all numbers (20 points) 5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to the OLI course modules, lab manual and/or use additional information resources. What effect does the acidic treatment have on enzyme activity? Did you get the expected results? Explain. (15 points) 6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back, how could you have improved your experiment? (10 points)
  • 6. 7. Include a list of references to all information sources used in APA format (5 points). Avoid Plagiarism It is very important to write with your own words. If you do copy one or two sentences directly (use sparingly), use quotation marks (") around the copied text, followed by an in- text reference. All information sources need to be included in the reference list and as in-text references. Plagiarism will be reported to the proper UMUC authority. Guidance on how to avoid plagiarism can be found here: Submission Submit your final applied lab project to both the TurnItIn website as well as in the assignment folder by the due date specified in the course schedule. Grading Your Final Applied Lab Project will be graded based on the following criteria (100 points total): Rubric Name: Final Applied Laboratory Project Criteria Excellent (90-100%) 100 points Good (75-89%) 89 points Adequate (50-74%) 74 points Unacceptable (0-49%) 49 points Outline 10 points (Due in week 7) Outline includes all the required components and planned experiment meets requirements for the assignment and is clearly
  • 7. and accurately described. Submitted on time. 9-10 points Outline missing one or two of the required components, and/or planned experiment does not meet one of requirements for the assignment and/or minor issues with clarity and accuracy. 7-8 points Outline missing several of the required components, and/or planned experiment does not meet several of the requirements for the assignment and/or major issues with clarity and accuracy. 5-6 points Outline missing most of the required components andplanned experiment does not meet the requirements of the assignment. 0-4 points Introduction, Question and Hypothesis (15 points) Background information about enzymes in general and about specific enzyme used in project is clearly and accurately written. Questions and hypothesis are specific, relevant and clearly stated. 14-15 points Background information about enzymes in general and about specific enzyme is somewhat unclear and/or inaccurate. Questions and hypothesis could be more specific, relevant and clearly stated. 11-13 points
  • 8. Missing background information about enzymes in general or about specific enzyme used in project or questions and hypothesis. 7-12 point Missing background information about enzymes in general and about specific enzyme used in project andquestions/hypothesis. 0-6 points Experimental Design, Materials and Methods (20 points) Experiment is designed to directly test the hypothesis; description of experiment is detailed and well written and includes all materials and methods used. 18-20 points Experiment is mostly designed to test the hypothesis and/or description of experiment is somewhat inaccurate and/or some information about materials and methods used is missing and/or minor problems with clarity an organization. 15-17 points Experiment is barely designed to test the hypothesis and/or description of experiment is inaccurate and/or most of the materials and methods used are missing and/or major problems with clarity an organization. 10-14 points Experiment is not designed to test hypothesis and/or description of experiment and materials and methods used are missing. 0-9 points Results (20 points)
  • 9. Results are clearly and accurately presented in a table and/or graph format. 18-20 points Results are presented, but minor problems with clarity and/or accuracy. 15-17 points Results are described, but major problems with clarity and/or accuracy and/or results not presented in table or graph. 10-14 points Results are not included 0-9 points Discussion of Results (15 points) A clear, accurate and well organized discussion of results that demonstrates good knowledge of enzymes. 14-15 points Minor problems with clarity, accuracy or organization and/or demonstrates some gaps in knowledge of enzymes. 15-17 points Major problems with clarity, accuracy or organization or demonstrates minimal knowledge of enzymes. 10-14 points Missing discussion of results, or major problems with clarity, accuracy or organization andmissing understanding of enzymes.
  • 10. 0-9 points Conclusion ( 10 points ) A well stated conclusion that is accurate and specific and directly related to the data collected. Clearly stated whether or not the results support the hypothesis. Relevant suggestion for improvement of experiment. 9-10 points Conclusion stated, but slightly inaccurate, and/or not directly related to data collected and/or suggestion for improvement of experiment not very relevant. 7-8 points Conclusion stated, but inaccurate and/or barely related to data collected and/or missing suggestion for improvement of experiment. 5-6 points Missing conclusion, only suggestion for improvement of experiment. 0-4 points Citations (5 points) All references used are included and in correct APA format, in- text references are included. 5 points All references are included, but a few minor mistakes in APA format, and/or missing in-text references. 4 points
  • 11. Missing one or several references and/or major mistakes in APA format. 3 point No references included or references not in APA format. 0-2 points Grammar and Spelling (5 points) Less than 3 minor spelling and grammatical errors 5 points Between 4 and 7 minor spelling and grammatical errors 4 points Between 8 and 12 spelling and grammatical errors 3 point More than 13 spelling and grammatical errors 0-2 points Overall Score Level 4 90 or more Level 3 75 or more Level 2 50 or more
  • 12. Level 1 0 or more Bottom of Form Final Project Outline The Effect of low pH on Enzyme Activity in Raw Meat Purpose: To recognize the impact of acid on based on the activity of enzymes Hypothesis: When acetic fluids are added to enzyme- substrate mixtures, enzyme activity is affected. Experiment: Observation of the yeast and temperature regulated hydrogen peroxide in the presence of food. Materials: · Name of enzyme you will use: Yeast · Name of organism (if applicable): Liver, carrot · The substrate: Hydrogen peroxide · Method for measuring enzyme activity: Circumference of balloons · Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples: length of exposure will be 1 min, 3 min, 5 min · The control(s) in the experiment: 3 Tubes filled with 10ml of catalase, 10ml of hydrogen peroxide and no acetic acid · Sample size: 3 tubes with 10ml of catalase, 10ml of hydrogen peroxide and each one with 1ml, 3ml, 5ml of acetic acid · Hypothesis: When acetic fluids are added to enzyme-substrate
  • 13. mixtures, enzyme activity is affected. · How you will present your data (table and/or type of graph): Table Procedure: 1. Cut 1 cm of meet and add to 200 ml cylinder 2. Add yeast into the same 200 ml cylinder 3. Add Hydrogen peroxide into the testing tube. 4. Cylinder and testing tube is kept in water both temperatures 80 degrees F. 5. Pour hot Hydrogen Peroxide in the cylinder that contains meat and yeast. 6. Volume of both cylinder and testing tube will be measured, before and after combining all items. 7. After 3 minutes the volume is measured again and recorded 8. Initial volume will be subtracted from final volume, to obtain the actual amount of foam. 9. Repeat by replace the acidic food with the alkaline one. 10. The data obtained will be used to create the table. Data and Analysis: observing the altered pH condition and the level of oxygen that is generated will collect Data Conclusion: the results of my exeriment References: Any references used (i.e.: internet, books, documentation)