Minibody is composed of a pair of single-chain Fv fragments which are linked via CH3 domains, and Fvs with distinct specificity, which paired to the former part through heterodimerization process. To promote the heterodimerization efficiency, single-residue mutations can be introduced into each CH3 domains to achieve the “knobs and holes” approach. Far more than that, additional cysteine residues can also be introduced into CH3 domains to stabilize the bispecific minibody structure.

1. minibody antibody
• Creative Biolabs possesses unchallenged experience in synthesis of
minibody and trimeric bispecific minibody (Tribi minibodies). Our
experienced scientists are committed to providing custom synthesis
strategy for your desired mini-antibody.
• Minibody is composed of a pair of single-chain Fv fragments which
are linked via CH3 domains, and Fvs with distinct specificity, which
paired to the former part through heterodimerization process. To
promote the heterodimerization efficiency, single-residue mutations
can be introduced into each CH3 domains to achieve the “knobs and
holes” approach. Far more than that, additional cysteine residues
can also be introduced into CH3 domains to stabilize the bispecific
minibody structure.

2. • As an enhanced version of minibody, Tribi minibody is an optimal tool
in targeting tumor cells. One of its chains, which is designed to
recognize tumor antigens via its two Fv fragments, while the other
chain possessing a Fv fragment takes charge of recruiting effector
cells, such as T cytotoxic cells or NK cells. With the addition of this
extra binding domain, the avidity of Tribi minibody is significantly
higher than that of the bispecific minibody. Thus, even at a lower
concentration, Tribi minibody is still able to perform a stronger
cytotoxicity than the corresponding bispecific minibody.

3. • Taking advantage of its abundant resources in biotechnology, Creative
Biolabs provides services not only a minibody or a Tribi minibody with
high-affinity and confirmed cytotoxicity, but also integrated a budget
and time-saving working procedure which can satisfy all of your
requirements in every step.