More Related Content Similar to Anti-idiotype Antibody Production (20) More from Creative Biolabs (20) Anti-idiotype Antibody Production1. Anti-idiotype antibodies
(anti-IDs) have specific
binding capabilities to an
idiotope of another
antibody.
Anti-idiotypic Antibody: Structure and Types
Approaches to Developing Anti-ID Antibodies
1
Immunized phage display
library technology:
Target antibody is utilized
to immunize animals.
Isolated immunized
library
Multiple anti-ID antibody discovery approaches
Highest possible affinity
Four types of anti-ID antibodies achievable
Large number of anti-ID antibodies for further
selection
Anti-ID antibody sequences are delivered
FEATURES:
ANTI-IDIOTYPE ANTIBODY
Creative Biolabs
Anti-idiotype
Antibody
Production
WHAT WE DO:
© 2021 Creative Biolabs All Rights Reserved. | Contact Us
α
β
γ
ε ε
δ
ζ
CD3
CD4 CD3
ζ
Antigen
Epitope
Paratope
Idiotype
Idiotopes
VH
VL
Fab
L-chain
Fc
H-chain
Complementarity deter-
mining regions (CDR), or
idiotype can determine the
antigen specificity of the
antibody. It can be the
actual antigen-binding site
or comprise variable region
sequences outside of the
antigenbinding site on the
antibody itself.
There are four main classi-
fications of detection
based anti-ID antibodies:
detect free antibody; detect
bound antibody; detect
total antibody; detect T cell
receptor.
Type 1
Type 2 Type 3 Type 4
Welcome
Target
antibdoy
Animal
immunization
Phage display
Screening & sequencing
Expression & purification
Fab antibodies
Conversion into Ig
Ig antibodies
Ab DNA library
Phage plasmid
Recombinant
phagemid
High affinity
mAbs
Affinity screening
Phage
display library
Amplify
E. coli
Screening
Myeloma cells
Hybridoma
Spleen B cell
Harvest mAbs
antibody is
packaged to phage library
and anti-ID antibodies can
then be generated by
biopanning. scFv/Fab
antibodies with an affinity
of 10-10
M could be
obtained.
Premade non-immunized
human phage display
library technology:
Phage display technology
in the absence of immuni-
zation suits perfectly for
the conformational epitope
targeting anti-idiotypic
antibodies, since in vitro
selection well maintains the
correctly folded conforma-
tion of target antibody.
Hybridoma technology:
scFv/Fab forms of target
antibodies are utilized to
immunize rats or mice in
the presence of isotype
matching control antibod-
ies for counter-selection.
Anti-idiotype Antibody Production
Phage Display Library Screening
Native™ Antibody Discovery
Antibody Characterization
Antibody Affinity Maturation
2
Hybridoma Technology
Premade Non-immunized Human Phage Display Library Technology
Immunized Phage Display Library Technology