Please help with an explanation method on how to approach these. I .pdf

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Please help with an explanation/ method on how to approach these. I get so confused. Complete the table below using + to indicate if the enzyme is synthesized and - to indicate if the enzyme will not be synthesized. Genotype symbols l = acl (the repressor gene) P = P_lac (the promoter), O = lacO (the operator), Z = lacZ (the beta = -galactosidase gene), and Y = lacY (the permease gene). The minus sign accompanying the symbol (e.g., I^1) indicates a mutant gene, the plus sign indicates wild-type/functional gene. O^c is the constituitive operator mutation (the same as O-) and l^-d genes make mutant subunits of the repressor protein (as opposed to I^1 which you can think of as not making a product) and I^s codes for the super-repressor protein, which will not release from the operator. Solution It is related to Lac operon and Here I, P & O are regulatory genes and Z & Y are structural coding genes. The expression of coding genes depends on regulatory genes. Generally O & P promotes expression of Z & Y. If I is functional, O & P cannot promote the expression of Z & Y. Inducer can remove the I effect and promotes Z & Y expression Based on the above words, we can tell weather the Z and Y are exprssed or Not. I have give some in below. Please find Genotype Inducer Absent Inducer Present B-Galactosidase or Lac-Z Permease or Lac-Y B-Galactosidase or Lac-Z Permease or Lac-Y A - - + + B - - - + C - - + - D + + + + E - - - - F - - + + G - - - - Genotype Inducer Absent Inducer Present B-Galactosidase or Lac-Z Permease or Lac-Y B-Galactosidase or Lac-Z Permease or Lac-Y A - - + + B - - - + C - - + - D + + + + E - - - - F - - + + G - - - -.

Please help with an explanation/ method on how to approach these. I get so confused. Complete
the table below using + to indicate if the enzyme is synthesized and - to indicate if the enzyme
will not be synthesized. Genotype symbols l = acl (the repressor gene) P = P_lac (the promoter),
O = lacO (the operator), Z = lacZ (the beta = -galactosidase gene), and Y = lacY (the permease
gene). The minus sign accompanying the symbol (e.g., I^1) indicates a mutant gene, the plus
sign indicates wild-type/functional gene. O^c is the constituitive operator mutation (the same as
O-) and l^-d genes make mutant subunits of the repressor protein (as opposed to I^1 which you
can think of as not making a product) and I^s codes for the super-repressor protein, which will
not release from the operator.
Solution
It is related to Lac operon and Here I, P & O are regulatory genes and Z & Y are structural
coding genes. The expression of coding genes depends on regulatory genes. Generally O & P
promotes expression of Z & Y. If I is functional, O & P cannot promote the expression of Z & Y.
Inducer can remove the I effect and promotes Z & Y expression
Based on the above words, we can tell weather the Z and Y are exprssed or Not. I have give
some in below. Please find
Genotype
Inducer Absent
Inducer Present
B-Galactosidase or Lac-Z
Permease or Lac-Y
B-Galactosidase or Lac-Z
Permease or Lac-Y
A
-
-
+
+
B
-
-
-
+

C
-
-
+
-
D
+
+
+
+
E
-
-
-
-
F
-
-
+
+
G
-
-
-
-
Genotype
Inducer Absent
Inducer Present
B-Galactosidase or Lac-Z
Permease or Lac-Y
B-Galactosidase or Lac-Z
Permease or Lac-Y
A
-
-
+

+
B
-
-
-
+
C
-
-
+
-
D
+
+
+
+
E
-
-
-
-
F
-
-
+
+
G
-
-
-
-

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Photos from our Spring Gala 2024. Thank you for making our 2024 Spring Gala unforgettable! Whether you attended or supported us with a donation, we extend our heartfelt thanks for celebrating School-Community Partnerships alongside ExpandED Schools. Special appreciation to our honorees, Rachel Skaistis and Cravath, Swaine, & Moore LLP, for their unwavering support in advancing educational equity. Your contributions have profoundly impacted countless young New Yorkers, and we’re proud to have you as part of our community. Our gratitude also extends to our Board of Directors, sponsors, city partners, community-based organizations, and the inspiring young people who joined us. Your support fuels our mission, and we’re endlessly thankful for your dedication. While we celebrate our gala’s success, our work continues. As you view the evening’s highlights, consider how you can further empower young New Yorkers and educators with your donation, no matter the size. Donate today: https://www.expandedschools.org/support-our-work/donate/

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1. Please help with an explanation/ method on how to approach these. I get so confused. Complete the table below using + to indicate if the enzyme is synthesized and - to indicate if the enzyme will not be synthesized. Genotype symbols l = acl (the repressor gene) P = P_lac (the promoter), O = lacO (the operator), Z = lacZ (the beta = -galactosidase gene), and Y = lacY (the permease gene). The minus sign accompanying the symbol (e.g., I^1) indicates a mutant gene, the plus sign indicates wild-type/functional gene. O^c is the constituitive operator mutation (the same as O-) and l^-d genes make mutant subunits of the repressor protein (as opposed to I^1 which you can think of as not making a product) and I^s codes for the super-repressor protein, which will not release from the operator. Solution It is related to Lac operon and Here I, P & O are regulatory genes and Z & Y are structural coding genes. The expression of coding genes depends on regulatory genes. Generally O & P promotes expression of Z & Y. If I is functional, O & P cannot promote the expression of Z & Y. Inducer can remove the I effect and promotes Z & Y expression Based on the above words, we can tell weather the Z and Y are exprssed or Not. I have give some in below. Please find Genotype Inducer Absent Inducer Present B-Galactosidase or Lac-Z Permease or Lac-Y B-Galactosidase or Lac-Z Permease or Lac-Y A - - + + B - - - +

2. C - - + - D + + + + E - - - - F - - + + G - - - - Genotype Inducer Absent Inducer Present B-Galactosidase or Lac-Z Permease or Lac-Y B-Galactosidase or Lac-Z Permease or Lac-Y A - - +

3. + B - - - + C - - + - D + + + + E - - - - F - - + + G - - - -