Mouse Genome Browser View for Psk9 gene Pcsk 9 exon/intron structure Mouse Genome Browser View for Apoc3 gene Apoc3 exon/intron structure Gene Editing Design after "looking" at the gene: You see that for at least one of the genes, there are several different annotated transcripts. This raises the possibility that putting a frameshift mutation into the "first" exon of the longest transcript may not actually knock out the gene, since it would not affect all of the transcripts. A similar situation could arise if you targeted an alternatively spliced exon. At the same time, you want to target as early as possible in the coding sequence so that a frameshift mutation will truncate/disrupt asmuch of the protein as possible. 1. Which exon (counting from right to left, since the gene is transcribed in the 1 point reverse direction) would you target in Pcsk9 to ensure that you knock out the gene? Your answer.