adding DNS. The pre-incubation step ensures that samples are at the correct temperature for the
entire enrymatic reaction. Note for groups testing optimal pH: ask your instructor for the correct
incubation temperature. You must also include at least one negative control that will also serve as
a blank for the spectrophotometer. Think earefully about how your control tube(n) will differ from
youe. experimental tubes? 5. Resuifs: Inclade Table I and Ciraph I for the maltose standard curve
in your final lab report. Complete Table 2 below with the data you collected after the DNS assay of
amylase activity. Usiny the maltose standard curve you cosstructed in Part II: a. Determine the
maltose produced in your experiment and insert it in this table. Show an example of this
calculation. b. Determine the amylase activity in each tube and insert it in this table. Show an
example of this calculation.Table 2: Data on the effect of &mpotbet on Aungal amylase setivity
Giraph 2: Graph the resulting Amylase Activity dats vs, your Independent Variable (usinig Excel)
and insert your graph in your report. Do not graph the control(s). Summary of results: State the
trend of each graph, describing how the dependent varable changes with the independent
variable. State the optimal condition for your enzyme..
adding DNS The preincubation step ensures that samples are.pdf
1. adding DNS. The pre-incubation step ensures that samples are at the correct temperature for the
entire enrymatic reaction. Note for groups testing optimal pH: ask your instructor for the correct
incubation temperature. You must also include at least one negative control that will also serve as
a blank for the spectrophotometer. Think earefully about how your control tube(n) will differ from
youe. experimental tubes? 5. Resuifs: Inclade Table I and Ciraph I for the maltose standard curve
in your final lab report. Complete Table 2 below with the data you collected after the DNS assay of
amylase activity. Usiny the maltose standard curve you cosstructed in Part II: a. Determine the
maltose produced in your experiment and insert it in this table. Show an example of this
calculation. b. Determine the amylase activity in each tube and insert it in this table. Show an
example of this calculation.Table 2: Data on the effect of &mpotbet on Aungal amylase setivity
Giraph 2: Graph the resulting Amylase Activity dats vs, your Independent Variable (usinig Excel)
and insert your graph in your report. Do not graph the control(s). Summary of results: State the
trend of each graph, describing how the dependent varable changes with the independent
variable. State the optimal condition for your enzyme.
