this document contains malarial parasite test and everything about the parasite

1. Title: Malaria Parasite Test
Aim: To determine the presence or absence of plasmodium spp
in the blood sample of a patient
Apparatus: Slide, Cover slip, Cotton wool, Methylated spirit,
microscope, immersion oil, tourniquet, needle & syringe, EDTA bottle
and lancet
Reagent: Leishman stain (blue black coloration)
Theory: Malaria is a mosquito borne infection disease of human and
other animals caused by plasmodium parasite. Commonly the disease is
transmitted b y a bite from an infected female anopheles mosquito
which introduce the organism from its saliva into a person’s circulatory
system

2. Procedure: The blood sample was collected and transferred into an EDTA
bottle to prevent the sample from clotting. Also the patient fingertip can be
pricked with the use of lancet and the thumb tip was pressed for blood
to drop. The blood was dropped on a slide and cover slip was gently used to
spread the sample on the slide to make a thin film.
It was stained with Leishman stain and was left for 3 minutes, then it was
diluted with water and also left for some few minutes and it was rinsed with
running water and also air dry or some minutes. Immersion oil was added
to the prepared stained slide and it was observed under the influence of
X100 objective lens
Observation: The result was to be positive if the presence of a chromatin
dot was observed which indicate that plasmodium species was in the film
while the result is negative, if there is no presence of chromatin dot in the
film.
Note: Plasmodium species are
Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae,
Plasmodium ovale. The specific parasite present in a patient having malaria
is Plasmodium falciparum
Conclusion: Conclusively, malaria parasite test is used to determine the
presence or absence of Plasmodium spp in the blood