3. Based on the tracing of current below measured through a nanopore made from -hemolysin
channel fused with cyclodextrin during translocation of free nucleotides in the paper of Clarke et al
(2009) (lecture notes "DNA Sequencing"), roughly estimate the time (in hours), which would be
required to sequence a single-stranded DNA molecule with a length of 100 million NTs assuming
that a. the -hemolysin + cyclodextrin protein is supplemented with exonuclease, which cleaves off
nucleotides right at the pore entrance, and b. the exonuclease cleavage rate is similar to the
nucleotide translocation rate through the pore (there is always a nucleotide inside the channel)..
3 Based on the tracing of current below measured through a .pdf
1. 3. Based on the tracing of current below measured through a nanopore made from -hemolysin
channel fused with cyclodextrin during translocation of free nucleotides in the paper of Clarke et al
(2009) (lecture notes "DNA Sequencing"), roughly estimate the time (in hours), which would be
required to sequence a single-stranded DNA molecule with a length of 100 million NTs assuming
that a. the -hemolysin + cyclodextrin protein is supplemented with exonuclease, which cleaves off
nucleotides right at the pore entrance, and b. the exonuclease cleavage rate is similar to the
nucleotide translocation rate through the pore (there is always a nucleotide inside the channel).
