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CYTOLOGY Lab.
1.   Single mitochondria, RER & Lipid droplet.
2.   Mitochondria ,RER & Secondery lysosomes.
3.   Mitochondria, SER & glycogen granules.
4.   T.S of centriole , Golgi complex &coated vesicle.
5.   Golgi complex.
6.   Peroxisome & Golgi complex.
7.   Golgi apparatus in epididymis
8.    Nucleus & RER (EM).
9.    Nuclear pore & rER continuous with the outer
      nuclear membrane(EM).
10.   Melanin pigment in negroid skin
11.   Glycogen granules in liver. Slide
12.   lipid droplet.(EM)
Single mitochondria, rER
& lipid droplet


Mitochondria.
Outer membrane and inner folded
membrane.
Cristae
Rough endoplasmic reticulum :
Consists of stacks of flattened
cisternae.
Membranous organells.
Polyribosomes on cytosolic surface of
the membrane
Mitochondria, rER & secondary lysosomes

Secondary
lysosomes.
Membranous
cell organelles..
Appears
heterogenous
due to presence
of digested
materials
sER , Mitochondria &
 glycogen granules

 Smooth endoplasmic reticulum.
Absence of ribosomes (smooth)
Cisternae is tubular in shape (not
flattened)
Mostly found in cells synthesizing steroid
hormones, cholesterol and triglycerides
like liver and endocrine cells.

Glycogen granules: appears
as collections
of electron-dense
particles (rosett appearance)
T.S. of centriole, Golgi
complex & coated vesicle
Centrioles are non-membranous
organelles.

centriole is a hollow cylinder with a dense
wall around an electron lucent central core.

Its wall is made up of 9 bundles of
microtubules, each bundle consists of 3
microtubules (triplets).
Golgi complex
Cis face and trans face, transfer
vesicles secretory vesicles.

Cis-face (immature or forming face)
which is usually convex. It is directed
towards rough endoplasmic reticulum
and its cisternae are highly
fenestrated.

 Trans-face (mature or secretory face)
is usually concave and is directed
towards the plasmalemma. The lumen
of its cisternae tends to be wider.
6- Nucleus & rER   NUCLEUS: The nuclear
                   envelope, chromatin, nucleolus, and
                   nuclear matrix.
Peroxisome & Golgi complex
 Spherical membranous organelles.
 Have a dense central region called crystaline core.
 Fine granular homogenous content.
Lipid droplets & cell membrane
A cell inclusion, Electron dense and homogenous, Variable in size.
Nuclear pores & rER continous with the outer
nuclear membrane
Golgi apparatus in epididymis
Dark stained area in cells of epididymis --- Supranuclear in position
Glycogen granules in liver
  A cytoplasmic inclusion, present in large amounts in liver cells
  (hepatocytes). Glycogen appears as dark acidophilic granules
Melanin pigments in negroid
skin
 A cytoplasmic deposit - Dark brown pigment. - In the
 epidermis of skin.

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Lab 1st year cytology

  • 2. 1. Single mitochondria, RER & Lipid droplet. 2. Mitochondria ,RER & Secondery lysosomes. 3. Mitochondria, SER & glycogen granules. 4. T.S of centriole , Golgi complex &coated vesicle. 5. Golgi complex. 6. Peroxisome & Golgi complex. 7. Golgi apparatus in epididymis
  • 3. 8. Nucleus & RER (EM). 9. Nuclear pore & rER continuous with the outer nuclear membrane(EM). 10. Melanin pigment in negroid skin 11. Glycogen granules in liver. Slide 12. lipid droplet.(EM)
  • 4. Single mitochondria, rER & lipid droplet Mitochondria. Outer membrane and inner folded membrane. Cristae Rough endoplasmic reticulum : Consists of stacks of flattened cisternae. Membranous organells. Polyribosomes on cytosolic surface of the membrane
  • 5. Mitochondria, rER & secondary lysosomes Secondary lysosomes. Membranous cell organelles.. Appears heterogenous due to presence of digested materials
  • 6. sER , Mitochondria & glycogen granules Smooth endoplasmic reticulum. Absence of ribosomes (smooth) Cisternae is tubular in shape (not flattened) Mostly found in cells synthesizing steroid hormones, cholesterol and triglycerides like liver and endocrine cells. Glycogen granules: appears as collections of electron-dense particles (rosett appearance)
  • 7. T.S. of centriole, Golgi complex & coated vesicle Centrioles are non-membranous organelles. centriole is a hollow cylinder with a dense wall around an electron lucent central core. Its wall is made up of 9 bundles of microtubules, each bundle consists of 3 microtubules (triplets).
  • 8. Golgi complex Cis face and trans face, transfer vesicles secretory vesicles. Cis-face (immature or forming face) which is usually convex. It is directed towards rough endoplasmic reticulum and its cisternae are highly fenestrated. Trans-face (mature or secretory face) is usually concave and is directed towards the plasmalemma. The lumen of its cisternae tends to be wider.
  • 9. 6- Nucleus & rER NUCLEUS: The nuclear envelope, chromatin, nucleolus, and nuclear matrix.
  • 10. Peroxisome & Golgi complex Spherical membranous organelles. Have a dense central region called crystaline core. Fine granular homogenous content.
  • 11. Lipid droplets & cell membrane A cell inclusion, Electron dense and homogenous, Variable in size.
  • 12. Nuclear pores & rER continous with the outer nuclear membrane
  • 13. Golgi apparatus in epididymis Dark stained area in cells of epididymis --- Supranuclear in position
  • 14. Glycogen granules in liver A cytoplasmic inclusion, present in large amounts in liver cells (hepatocytes). Glycogen appears as dark acidophilic granules
  • 15. Melanin pigments in negroid skin A cytoplasmic deposit - Dark brown pigment. - In the epidermis of skin.