1. Laura Ann Kelley
San Diego,CA 92124
lakelley310@gmail.com
Profile
Experienced Molecular and Cell Biologist with 10+ years of experience in industry and academia. Currently
working as an Associate Scientist for Eli Lilly & Company where I employ a variety of molecular and cellular
techniques in drug discovery and development.
 Proficientin flow cytometry sample preparation, acquisition (LSRII,LSRFortessa, FACSCanto,
FACSAria, CyTof) and analyses (FlowJo,FACSDiva)
 96 and 384 well High Throughput Screening Assay development
 Tissue/cell culture of suspension and adherent cell lines and primary mammalian cells
 In vitro assays (Bioplex 200, MSD, Cell Proliferation,Cell Death, Necrosis & Apoptosis, Reporter
Gene Assays, Functional Bioassays)
 Molecular cloning techniques (DNA/RNA extraction and quantification, primer design, vector
design, ligation restriction digestion, PCR, DNA sequencing, bacterial transformation, protein
transfection),
 General molecular, protein and biochemistry techniques (chemical and radio isotopic proliferation
and cytotoxicity assays,ELISA, Western blotting, protein purification techniques by metal affinity
chromatography, SDS-PAGE /Agarose gel electrophoresis)
 Human and murine immune cell isolation (MACS) and characterization from pBMC and whole blood
 Experience in virology techniques for transduction and working with live MCMV forpre-clinical
vaccinemodels
 Additional training (Animal care and use program, Mouse/Rat handling techniques, Blood-borne
pathogen training, Radiation safety training )
Professional experience
Eli LillyandCompany,San Diego, CA, Associate Consultant Biologist (August 2007-present)
 Responsible forprocessing and analysis of large multicolor flow cytometry panels involving surface
and intracellular staining of fresh and cultured human PBMCs, mouse splenocytes and lymph nodes
 Optimize human mixed lymphocytereactions for bio-assay of antibodies
 PerformHigh Throughput flow cytometry binding assays
 Performin vitro cell based assays (ELISA,Luminex) for cytokines,chemokines, and
immunoglobulin from cells and condition medium
 Train colleagues on FACSDiva software, proper instrument use, and education in multicolor flow
cytometry acquisition and analysis.
 Optimize single cell PCR, high throughput cloning and expression protocols forfull length
antibodies in mammalian cells and demonstrate and troubleshoot the applicability and diversity of
scPCR across a wide variety of targets for pharmaceutical drug development
 Support team projects by assisting with desired molecular biology work.

2. Cedars-SinaiMedical Center,LosAngeles, CA, ProjectResearch Associate II (June2006-August 2007)
 Tissue culture, processing and analysis of tumor celllines used in the development of antibody
therapeutics forpre-clinical drug discovery in prostate cancer research
 Transient transfection of suspension and adherent mammalian cell lines and gene and expression
of secreted proteins to identify prostate cancerprotein markers as prognostic factors
 Western Blot analyses
 Cell based proliferation and cytotoxicity assays
 Cloning of targeted genes into expression vectors
Amcyte DiabetesInc.,Santa Monica, CA, ProjectResearch Associate II (October2004-June 2006)
 Progenitor stem cell culturing, proliferation and maintenance (adult human and neonatal porcine)
 Sample preparation for DNA, RNA (RT-PCR) and immunohistochemistry assays and
immunofluorecent staining
 Sample preparation for FACS sorting
UniversityofCalifornia,SanDiego,ResearchAssociate/Asst Lab Manager (April 2003-October 2004)
 Infectionand propagation of MCMV in culture
 Viral preparation and titers transfection and gene expression of viral antigenic proteins
 Plasmid DNA preparation and dosing for in vivovaccination studies
Education
UniversityofNevada,Las Vegas
 M.S., Biology,May 2002.
 B.S., Biology,May 1998
Publications/Posters
Morello CS, KelleyLA,Munks MW,Hill AB, Spector DH. Cytomegalovirus genes encoding homologs of
human cytomegalovirusUL54 (DNA polymerase) and UL105 (helicase) elicits strong CD8 T-cellresponses
and is protective against systemic challengeJ Virol. 2007 Jul;81(14):7766-75. Epub 2007 May 16.
Monica Florio, ErikPong, Laura Kelley,Wen-GhihTsang and Aleksandar Francki. Growth Factor-mediated
Activationof Akt1 in Adult Primary Human Pancreatic Cells Enhances the Survivaland Proliferationof
Endocrine Lineage Cells. AmCyte Inc., Santa Monica, California 90404, USA.
Keystone Symposia Poster. Pancreatic Islets: From Development to Transplantation, February 23-28,
2006, Sagebrush Inn and Conference Center, Taos, New Mexico

3. Morello CS, YeM, Hung S, KelleyLA,Spector DH. Systemic priming-boosting immunization with a
trivalent plasmid DNA and inactivated murine cytomegalovirus (MCMV) vaccineprovides long-term
protection against viral replication following systemic or mucosal MCMV challenge.
J Virol. 2005 Jan; 79(1):159-75.
Riddle, BR, SJ Mantooth, L Kelley,and T Lawlor.Population structure and mountain-top phylogeography of
the Tamiasumbrinus species complex in western North: expansion, migration, and diversificationin
response to changing climates. American Society of Mammologists, Lubbock,TX;2003.
Laura A. Kelleyand Brett R. Riddle, Is Palmer’s chipmunk (T. palmeri) a distinct evolutionarily significant
unit (ESU)withinthe Uinta chipmunk (T. umbrinus) species group? Department of Biological Sciences,
University of Nevada, Las Vegas, Las Vegas, Nevada 89154-4004. Annual meeting of the American Society
of Mammalogists, Missoula, MT; 2001.