1. Omar Quintero-Monzon, Ph.D. quintero.monzon@gmail.com 1 / 3
Omar Quintero-Monzon, Ph.D.
PROFESSIONAL PROFILE
 Comprehensive knowledge of protein biochemistry and glycobiology with 4+ years in biotech industry.
 Extensive experience in antibody drug development, including leading projects and managing CROs to
access novel technologies.
 Broad biochemical experience including protein purification and characterization, preclinical assay
development and structural biology.
 Proven track record of initiating and introducing innovative technologies to laboratories.
PROFESSIONAL EXPERIENCE
Scientist, Discovery Therapeutics 2016 - present
SHIRE, Lexington, MA
 Developed Octet based assays to evaluate sialic acid composition of recombinant glycoproteins in order to
optimize expression conditions.
 Developed UPLC based assay for isolation and quantitation of monosaccharides from glycoproteins.
Scientist, Biologics Drug Discovery 2011- 2015
BIOGEN, Cambridge, MA
 Co-led an antibody drug discovery project for ALS and identified lead therapeutic candidates.
 Developed robust purification methods for antibodies and their binding partners, including aggregation-prone,
RNA-binding proteins with intrinsically disordered domains. Consistently delivered highly pure, functional
protein.
 Developed novel biomarker assays using ELISA/MSD/Singulex platform. For 2 challenging drug targets, a
>50-fold improvement in sensitivity over the standard assay allowed for their detection in plasma and cerebral
spinal fluid.
 Introduced and established transmission electron microscopy tools to understand antibody binding epitope
and specificity. This contribution provided key insights into the mechanisms of action of three antibody drugs.
 Co-authored 2 research papers and submitted 1 patent application.
 Responsible for the performance evaluation and career growth of associate scientist during 3-year manager
role.
NIH-Postdoctoral Fellow, Neurology Department, Brigham and Women’s Hospital 2007- 2010
HARVARD MEDICAL SCHOOL, Boston, MA
 Developed robust enzymatic assays based on single amino acid resolution electrophoresis. Demonstrated
that processivity and total activity of the enzyme complex gamma-secretase are independent.
 Led a team of 5 scientists that integrated 30+ constructs of membrane-bound gamma-secretase into CHO
cells, and expressed and purified the corresponding protein complexes.
 2 years of mentoring a lab technician and 2 years of mentoring thesis work of a Harvard University
undergraduate student.
Graduate Student, Biochemistry Department 2001- 2007
BRANDEIS UNIVERSITY, Waltham, MA
 Cloned, expressed and purified >10 constructs each of the recombinant proteins Abp1 and Srv2, among other
cytoskeleton proteins. Developed biochemical assays to define the functional significance of three protein-
protein interactions (actin-Abp1, actin-Srv2 and cofilin-Srv2) for the kinetics of actin polymerization.
 Applied structure- based, site directed mutagenesis to map the binding interface of the three protein pairs
above, using biochemical and Saccharomyces cerevisiae genetic techniques.
Research Technician, Biochemistry Department 2000- 2001
BRANDEIS UNIVERSITY, Waltham, MA
 Purified and characterized recombinant Shaker potassium channels isolated from CHO cells.
 Performed initial transmission electron microscopy (TEM) screening of the SNARE protein complex.

2. Omar Quintero-Monzon, Ph.D. quintero.monzon@gmail.com 2 / 3
RESEARCH SKILLS
Biochemistry
HPLC Size Exclusion Chromatography combined with Multi-Angle static Light Scattering (SEC-MALS),
dynamic light scattering (DLS) and analytical ultra-centrifugation (AUC) to evaluate polydispersity and
molecular weight of proteins. Expression and purification of soluble or membrane associated proteins from
E.coli, S. cerevisiae and CHO cells. Capillary electrophoresis (LabChip GXII and Peggy Sue CE-SDS)
characterization of proteins. FPLC - Akta with IMAC, ion-exchange, HIC, affinity purification and SEC.
Traditional chromatography of tagged and untagged proteins. Generation of pyrogen free proteins for
animal studies. Solubilization of intrinsically disordered protein/domains. UREA denaturation experiments,
SDS-PAGE/Western blotting. Peptide and glycan HPLC. Interpretation of hydrogen/deuterium exchange
mass spectrometry data. Sucrose gradients and preparative electrophoresis.
Ligand Binding Assays
Multi-format immunoassays, ELISA, MSD, Singulex, Octet, immuno-precipitation. Chemical labelling and
crosslinking of proteins (both amine and cysteine chemistry). Fluorescence-based activity and binding
assays.
Molecular Biology, Cell Biology and Genetics
Basic recombinant DNA techniques, cloning by yeast recombination, PCR, mammalian cell culture and
gene integration, yeast genetics (tetrad analysis, gene integration, genotypic characterization),
immunofluorescence microscopy.
Structural Biology
Transmission electron microscopy analysis of negatively stained protein samples (on Philips CM12 and FEI
Morgagni 286, Tecnai™ G² Spirit BioTWIN and JEOL 1200EX microscopes), single particle averaging and
reconstruction of protein complexes using IMAGIC software. Immuno-gold staining methods in TEM.
Glycobiology
Multidimensional chromatographic mapping of N-glycans, oligosaccharide derivatization with fluorescent
tags and purification by HPLC (C8, C18, amine), carbohydrate preparative electrophoresis, lectin based
chromatography of N-glycans, exoglycosylase profile characterization of oligosaccharides.
EDUCATION
Ph.D. Department of Biochemistry 2007
Brandeis University, Waltham, MA
B.S./M.S. Department of Chemistry 1995
University of Havana, Havana, Cuba
AWARDS
2007 National Institute of Aging, NIH Research Diversity Supplement Award.
2004 National Institute of General Medical Sciences, NIH Research Diversity Supplement Award.
PATENTS
Roger N, Hock C, Barenco Montrasio MG, Montrasio F, Grimm J, Baeriswyl JL, Weinreb P, Coomaraswamy J,
Quintero-Monzon O.
TDP-43 specific binding molecules. US patent application 20140255304. applied October 26, 2012, filed
September 11, 2014
PUBLICATIONS
Sevigny J, Chiao P, Bussiere T, Weinreb P, Williams L, Maier M, Dunstan R, Salloway S, Chen T, Ling Y,
O'Gorman J, Qian F, Arastu M, Mingwei Li , Chollate S, Brennan M, Quintero-Monzon O, Scannevin R, Arnold
H, Engber T, Rhodes K, Ferrero J, Hang Y, Mikulskis A, Grimm J, Hock Ch, Nitsch R. The antibody
aducanumab reduces beta-amyloid plaques in Alzheimer’s disease. Nature 2016, 537, 50-56.
PUBLICATIONS (cont.)

3. Omar Quintero-Monzon, Ph.D. quintero.monzon@gmail.com 3 / 3
Pepinsky RB, Arndt JW, Quan C, Gao Y, QUINTERO-MONZON O, Lee X, Mi S.
Structure of the LINGO-1-anti-LINGO-1 Li81 antibody complex provides insights into the biology of LINGO-1
and the mechanism of action of the antibody therapy. J Pharmacol Exp Ther. 2014, Apr 22.
QUINTERO-MONZON O, Martin MM, Fernandez MA, Cappello CA, Krzysiak AJ, Osenkowski P, Wolfe MS.
Dissociation between the processivity and total activity of gamma-secretase: implications for the mechanism of
Alzheimer's disease-causing presenilin mutations. Biochemistry 2011, 50, 9023-35
QUINTERO-MONZON O, Jonasson EM, Bertling E, Talarico L, Chaudhry F, Sihvo M, Lappalainen P, Goode BL.
Reconstitution and dissection of the 600-kDa Srv2/CAP Complex: roles for oligomerization and Cofilin-Actin
binding driving actin turnover. J Biol Chem. 2009; 284, 10923-34.
QUINTERO-MONZON O, Rodal AA, Strokopytov B, Almo SC, Goode BL.
Structural and functional dissection of the Abp1 ADFH actin-binding domain reveals versatile in vivo adapter
functions. Mol Biol Cell. 2005, 16, 3128-39. (highlighted in ASCB Newsletter, 2005).
Hong S, QUINTERO-MONZON O, Ostaszewski BL, Podlisny DR, Cavanaugh WT, Yang T, Holtzman DM, Cirrito
JR, Selkoe DJ.
Dynamic analysis of amyloid β-protein in behaving mice reveals opposing changes in ISF versus parenchymal
Aβ during age-related plaque formation. J Neurosci. 2011, 31, 15861-9
Chauldhry F., Little K., Talarico L., QUINTERO-MONZON, O, Goode, BL.
A central role for the WH2 domain of Srv2/CAP in recharging actin monomers to drive actin turnover in vitro and
in vivo. Cytoskeleton. 2010, 67, 120-133.
Mattila PK*, QUINTERO-MONZON O*, Kugler J, Moseley JB, Almo SC, Lappalainen P, Goode BL.
A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of
Srv2/cyclase-associated protein. Mol Biol Cell. 2004, 15, 5158-71 (*Both authors contributed equally).
Bertling E, QUINTERO-MONZON O, Mattila PK, Goode BL, Lappalainen P.
Mechanism and biological role of profilin-Srv2/CAP interaction. J Cell Sci. 2007; 120, 1225-34.
Garcia R., Cremata J.A., QUINTERO-MONZON O, Montesino R., Benkestock K., Stahlberg J.
Characterization of protein glycoforms with N-linked neutral and phosphorylated oligosaccharides: studies on
the glycosylation of endoglucanase 1 (Cel7B) from Trichoderma reesei. Biotechnology and Applied
Biochemistry. 2001, 33, 141-152.
Valdes R., Ibarra N., Gonzalez M., Alvarez T., Garcia J., Llambias R., Perez CA, QUINTERO-MONZON O, Fischer
R. CB.Hep-1 hybridoma growth and antibody production using protein-free medium in a hollow fiber bioreactor.
Cytotechnology. 2001, 35, 145-154.
Betancourt L.H.; Garcia R.; Gonzalez J.; Montesino R.; QUINTERO-MONZON O, Takao T.; Shimonishi Y.;
Cremata J.A. Dextranase (alpha-1,6 glucan-6-glucanohydrolase) from Penicillium minioluteum expressed in
Pichia pastoris: Two host cells with minor differences in N-glycosylation. FEMS Yeast Research. 2001, 1, 151-
160.
Montesino R., Nimtz M., QUINTERO-MONZON O, García R., Falcón V., Cremata J. A.
Characterization of the oligosaccharides assembled on the Pichia pastoris expressed recombinant aspartic
protease. Glycobiology. 1999, 9, 1037-43.
QUINTERO-MONZON O, Montesino R and Cremata J.A.
Two-Dimensional mapping of 8-amine-1,3,6-naphthalene trisulfonic acid derivatives of N-linked neutral and
sialyl oligosaccharides. Anal Biochem. 1998, 254, 23-32.
Montesino R, García R, QUINTERO-MONZON O, Cremata JA
Variation in N-Linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic Yeast
Pichia pastoris. Protein Expression and Purification. 1998, 14, 197-207.