Mohamed Kamal SalehHome: Haj Shaaban Tower, level 10                     University: Zoology Dept, Faculty of Science     ...
Work ExperiencesJuly 2010- present     Lecturer of Molecular Biology in the department of Zoology, Faculty of Sciences    ...
PublicationsKamal, M., Shaaban, AM., Zhang, L., Wailker, C., Gray, S., Thakker, N., Speirs, V., and Bell, SM.(2010) Loss o...
Teaching skills:2007-2010          Demonstrator, University of Leeds, UK.                                          Supervi...
Grants and Awards  2010            The Bibliotheca Alexandrina post-doctoral research grant  2009            Best achiever...
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Mohamed kamal saleh_c_v (update oct 2011)

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Mohamed kamal saleh_c_v (update oct 2011)

  1. 1. Mohamed Kamal SalehHome: Haj Shaaban Tower, level 10 University: Zoology Dept, Faculty of Science Bata, University of Benha Benha Banha E-mail: mk_saleh1980@yahoo.com Mobile: 01117532985ResearchMy main research interests lie in the field of molecular biology, more specifically in tumourpathology and cell biology. During my post doctoral training in the University of Leeds, I haveexamined the biochemistry of V (D) J recombination reactions, a process responsible for antibodiesproduction, and if gone wrong it causes lymphomas and leukemias. Achieving this project providedme strong experience in the fields of chromatin research and immunology. Moreover I gainedextensive experiences in molecular cloning, site directed mutagenesis, western blotting and real timePCR.On the other hand, I have investigated the prognostic value and functional roles of a novel tumoursuppressor gene (CSMD1) for my PhD. Conducting such a project has given me wide range of skillsand experiences. I have mastered a wide range of molecular and cell biology techniques. I haveestablished shRNA stable cell lines, using cloning, to study the biological consequences of silencingCSMD1 expression. Interestingly, I have found that loss of CSMD1 expression resulted in increasedcell proliferation, migration and invasion. In contrast, down regulation of CSMD1 expression causeda decrease in cell adhesion and disruption of breast duct formation. Moreover, I have extensiveexperience of immunohistochemistry and statistical analysis. I have identified that loss of CSMD1protein expression is associated with poor prognosis in breast cancer patients.Education2010 PhD” Characterization of CSMD1 in Cancer” Leeds Institute of Molecular Medicine, School of Medicine, University of Leeds, UK. To functionally characterise CSMD1, I have stably silenced CSMD1 expression in four cancer cell lines, using plasmid based RNAi (shRNA). Using CSMD1 shRNA stable cell lines, I have performed wide range of cell based assays to study cell morphology, size, proliferation, adhesion, migration and invasion. In order to study the role of CSMD1 in cell differentiation and duct formation of the mammary gland, I have established 3D matrigel models, using MCF10A CSMD1 stable cell line. Using immunofluorescence, I have studied cell cytoskeleton, polarity and apoptosis in the 3D models. In order to evaluate CSMD1 as a prognostic factor, I have stained paraffin embedded tissue microarrays for more than 400 breast cancer patients using immunohistochemistry. Using univariate and multivariate analyses, I found that loss of CSMD1 expression was associated with poor patient’s outcome and considered an independent biomarker in breast cancer.1997.2001 BSc in Zoology (First class honour degree), University of Benha, Egypt. 1
  2. 2. Work ExperiencesJuly 2010- present Lecturer of Molecular Biology in the department of Zoology, Faculty of Sciences , University of Benha, Egypt. The main responsibilities of this post are to teach the molecular biology and molecular biology related courses to the undergraduate students. Also, to supervise demonstrators and teaching assistants while they deliver the practical to students. In addition, supervising final exams and marking students answer sheets. Moreover, I am supervising a Master student, who is conducting a research project under the title “Biochemical markers of hepatocellular carcinoma in hepatitis C virus infection combined with hepatic schistosomiasis”. In this project we aim to determine a better management tool for patients with liver cancer. On the other hand, I am conducting a research project funded by bibliotheca Alexandrina, Egypt and I am writing up a research proposal to be submitted to the STDF (under the reintegration programme). This project will investigate the causes and potential drugs of breast cancer in Egyptian women. If successful, the fund will cover me (a principle investigator) and two non phD holders (research assistants).May 2010- present Post doctoral research fellow in the institute of Molecular and cellular Biology, Faculty of Biological Sciences, University of Leeds, England, UK. In this post I have been investigated the molecular biology and biochemistry of the V(D)J recombination reactions. These reactions are involved in the production of antibodies and T cell receptors. The By-product of V(D)J recombination was thought for long time to be degraded during cell division. Recently, a group of researchers has shown that these By-products could reintegrate into the genome and cause genomic instability, which eventually will cause cancer (Lymphomas and leukaemia). Our aim is to understand the molecular mechanisms of these reintegrations and how to inhibit them. We believe that the results of this project will be the ground base for designing anti- Lymphomas and leukaemia drugs.September 2006- May 2010 PhD student in the Leeds Institute of Molecular Medicine (LIMM), School of Medicine, University of Leeds, England, UK. This PhD studentship costed arround 1.5 million egyptian pounds and was funded by the Egyptian government.February 2002- September 2006 Demonstrator in the Zoology Department, Faculty of Sciences, Benha University, Egypt. 2
  3. 3. PublicationsKamal, M., Shaaban, AM., Zhang, L., Wailker, C., Gray, S., Thakker, N., Speirs, V., and Bell, SM.(2010) Loss of CSMD1 expression is associated with high tumour grade and poor survival in invasiveductal breast carcinoma. Breast Cancer Res Treat. 2010 Jun;121(3):555-63Towns, K., Kipioti, A., Long, V., McKibbin, M., Ehsani, P., Springell, Kelly., Kamal, M., Ramesar,R., Mackey, D., Moore, A., Mukhopadhyay, R., Webster, A., Black, G., Sullivan, J., Pierce, E.,Beggs, J., and Inglehearn, C (2010) Prognosis for splicing factor PRPF8 Retinitis pigmentosa andcorrelation between human and yeast phenotypes. Human Mutation. 2010 May;31(5):E1361-76.Kamal, M., Toomes, C., Holliday, D., Speirs, V., and Bell, SM. (2011) Loss of CSMD1 expressionenhances cell proliferation, migration and invasion but disrupts cell morphology, adhesion andmammary duct formation. Cancer Research (submitted).Richardson, J., Shaaban AM., Kamal, M., Alisary, R., Ellis, I., Speirs, V., Green, A., Bell, SM.(2010) Identification of reduced MCPH1 expression in aggressive breast cancer. Breast Cancer ResTreat. 2011 Jun;127(3):639-48Kamal, M., Raiahi, S., Grabitchi, H., and Bell, SM. (2011) Loss of CSMD1 expression is associatedwith poor prognosis in gastric carcinoma. J Pathology (in preparation)Kirkham, C., Kamal, M., and Boyes J. (2011) A Potential New Mechanism by which V(D)JRecombination Triggers Genome Instability (in preparation)Laboratory skills:Molecular biology such as DNA and RNA extraction, cloning (annealing, ligation, and transformationreactions), plasmid DNA preparations (mini and large scales preps), Sequencing, PCR, genotyping,reverse transcription reaction (RT), digesion reactions, gel extraction and real time PCR). Cell culturetechniques such as maintaining and freezing cells, DNA and RNA transfection, cell-based assays suchas adhesion, proliferation, migration, invasion, live cell labelling, co cultures and 3D models),immunoflourescence, microscopy (epifluorescence, confocal and electron microscopy), and live-cellimaging microscopy. Mice breading and dissection, Histology, and immunohistochemistry. I alsohave extensive experiences in western blotting, site directed mutagenesis and have attendedworkshops in ELISA. 3
  4. 4. Teaching skills:2007-2010 Demonstrator, University of Leeds, UK. Supervise students to carry out the practical sessions of histology, molecular biology and cell biology modules, along with supporting and helping other students in the laboratory. Marking student’s performance sheets. Supervising university placement students in Dr. Bell’s group. 2002- 2006 Demonstrator, University of Benha, Egypt. Delivering tutorials in histology, cell biology and molecular biology to groups of 25 undergraduate students.Computer experience Microsoft Word, Excel, PowerPoint, SPSS, real-time PCR software (for the ABI 7500 and 7700 instruments), ScanScope, Volocity (time-lapse imaging software), Adobe Photoshop, DreamWeaver, SIPR and Science Warehouse purchasing systems. International Computer Driving Licence (ICDL).Conferences and workshops 2011 Academic tutor for the credit hour teaching system, Benha, Egypt. 2011 Yorkshire Cancer Research annual meeting, Harrogate, England (Poster) 2011 London annual chromatin meeting, Imperial college, London 2010 1st British Breast Cancer Research Conference, Nottingham, England (Oral presentation) 2010 Breast Cancer Campaign annual meeting, London (Poster) 2009 Egyptian Cultural Bureau annual scientific meeting, London (oral presentation) 2009 Annual North of England cell biology meeting, York (poster). 2009 Beatson International Cancer Conference, Glasgow (poster). 2008 Faculty of Medicine and Health annual meeting (oral presentation). 2008 Joint Meeting of the Pathological Society of Great Britain and Ireland and the Dutch Pathological Society (2 posters). 2008 Breast Cancer Campaign (BCC) annual meeting, London. 2007 Intensive statistical course, University of Leeds. 2006 Health and safety course for working with genetically modified organisms, University of Leeds. 2005 Electron microscopy workshop, Central laboratories, University of Ain shams, Cairo, Egypt. 2004 PCR workshop, University of Ain shams, Cairo, Egypt. 4
  5. 5. Grants and Awards 2010 The Bibliotheca Alexandrina post-doctoral research grant 2009 Best achievers for the year (Egyptian embassy, London).LanguagesNative Arabic, fluent English and basics of FrenchInterests Reading and swimming Clean full UK driving licence Clean full Egyptian driving licence 5

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