3. PRINCIPLE
Acid treatment denatures fragmented DNA
The lysing solution removes most of the nuclear
proteins
In the absence of massive DNA breakage produces
nucleoids with large halos of spreading DNA loops,
emerging from a central core.
The nucleoids from spermatozoa with fragmented
DNA either do not show a dispersion halo or the halo
is minimal.
4.
5. The Sperm Chromatin Dispersion Test: A Simple
Method for the Determination of Sperm DNA
Fragmentation
Presence of extensive DNA breaks,
High amounts of ssDNA
Suppressing the generation of DNA dispersion
The results suggest that ssDNA interacts within the
sperm head in such a way that the removal of most
nuclear proteins by lysing solutions does not result in
dispersion of the DNA fragments.
6. SPERM CHROMATIN STRUCTURAL ASSAY
Treatment with an acid solution
That opens up the DNA strand at the breaks or
fragmented regions.
The dye Acridine Orange binds
Double stranded DNA (dsDNA) and single stranded
DNA (ssDNA) and fluoresces green or red
7.
8. DNA FRAGMENTATION AND TREATMENT
Antibiotics
Antioxidants
Diet
Quitting smoking, alcohol
Varicocele surgery
Testicular aspiration of sperm
Avoid exposure of toxic
Scrotal cooling
Treatment for diabetes
9.
10. REFERENCE
Clinical application of sperm chromatin structure assay in diagnosis and
treatment of infertility
Comparison and variation of % dna fragmentation index (%dfi) between
sperm chromatin structure assay (scsa®) and sperm chromatin dispersion
(scd) tests
Comparison of the halosperm® test kit with the sperm chromatin structure
assay (scsa®) infertility test in relation to patient diagnosis and prognosis
donald p. Evenson, +and regina wixon
Comparison of chromatin assays for DNA fragmentation evaluation in
human sperm KAZIM R. Chohan, jeanine t. Griffin, marie lafromboise,
christopher j. De jonge, and douglas t. Carrell
Evaluation of sperm DNA fragmentation using multiple methods: a
comparison of their predictive power for male infertility - aamir javed1,
muralidhar srinivasaih talkad2, manjula kannasandra ramaiah
The clinical utility of sperm DNA integrity testing - American socity of
reproductive medicine
