Analysis Analysis Analysis Analysisof the entire entire entire protein protein proteinproteincomplementcomplement complement complement of acell, cell, tissue, tissue, tissue, or organism organism organism under under aspecific, specific, specific, defined defined set of conditions conditions conditions . • Relies Relies Relies on 3basic technological technological technological technological technological cornerstones cornerstones cornerstones cornerstones • MethodMethod MethodMethod to fractionatefractionate fractionatefractionate fractionatefractionate complexcomplex complex protein/protein/ protein/ protein/ peptide peptide peptidemixturesmixtures mixtures • MS to acquire acquire the data data necessary necessary to identify identify identifyidentifyindividual individual individual individualproteins proteins • Bioinformatics Bioinformatics Bioinformatics Bioinformatics Bioinformatics Bioinformatics Bioinformaticsto analyze analyze and assemble assemble the MS data

1. MASS SPECTROMETRY BASED
PROTEOMICS ANALYSIS
WEBINAR

2. MASS SPECTROMETER (MS)
• Mass Spectrometry is a powerful analytical
tool.
• Virtually ubiquitous research tool.
• MS technologies continuously evolving.
• Miniaturization.
PALM POTABLE MASS
SPECTROMETER

3. MASS
SPECTROMETER
PROTEOMICS
METABOLOMICS
LIPIDOMICS
BIOMARKER
DISCOVERY
PTM ANALYSIS
INTERACTOMICS
MASS SPECTROMETER IN “OMICS” ERA

4. NEED OF MASS SPECTROMETER
CLINICAL STUDIES
ENVIRONMENTAL STUDIES
FORENSIC APPLICATIONS
GEOLOGICAL STUDIES
PHARMACEUTICAL ANALYSIS

5. MASS SPECTROMETER:
A BOON TO INDUSTRY
FOOD CHEMICAL PETROLEUM
BIOTECHNOLOGY NUCLEARSPACE SCIENCE

6. DETECTING
CORONAVIRUS USING MS
• Institute of genomics and integrative biology
(IGIB) and National centre for disease control
(NCDC) detected novel coronavirus with 95%
sensitivity and 100% specificity.

7. Technology
advancements
Life science research
Rapid evolution
of healthcare
Increasing support and
funding from governments
Growing end-user
industries
MASS
SPECTROMETRY
MARKET: STRONG
GROWTH

8. The global mass spectrometry market is
projected to grow

9. WHAT IS MASS SPECTROMETRY?
• Mass spectrometry (MS) is a technique for production of charged molecular species and
their separation by magnetic and electric filed based on mass to charge ratio.

10. RELEVANCE OF
MASS
SPECTROMETRY
Detection
Identification
Structure elucidation
Quantification

11. MAIN DOMAINS OF MASS SPECTROMETRY
Technical Issue
Sample introduction
Ionization methods
Vacuum systems
Types of Mas analyzers
Combinations of mass analyzers
Modes of operation
Interpretation of mass spectra
Fragmentation pathways
Characteristics ions
Rules for interpretation
Applications
Identification
Quantitation
Fundamentals
Ionization process
Internal energy
Isotopic distribution
Isotopic mass
MASS
SPECTROMETER

12. UNIQUE FEATURES OF MS
Molecular
specificity
Detection
sensitivity
Versatility &
wide
applicability
Analysis of
complex
samples

13. Save time
Handle lot of
samples
Customizable
Power Technique
Perform Omics
experiment

14. BASIC COMPONENTS OF MASS SPECTROMETER
Sample
inlet
Ion source
Mass
analyzer
Detector
Data
system
• Gas Phase
• Electron Ionization
• Chemical Ionization
• Photoionization
• Solution Phase
• Electrospray
• Atmospheric Pressure (PI)
• Atmospheric Pressure (CI)
• Solid Phase
• Matrix-assisted laser desorption
• Plasma desorption
• Fast Atom Bombardment
• Time-Of-Flight (TOF)
• Quadrupole (Q)
• Ion trap
• Fourier Transform-ion Cyclotron Resonance (FT-ICR)
• Orbitrap
• Magnetic sector
• Direct infusion
• Gas Chromatography
• Liquid Chromatography
• Capillary electrophoresis
Vacuum system
• Electron multiplier
• Faraday cup
• Photomultiplier conversion dynode
• Array detector

15. KEY STEPS
• Ionisation
• Acceleration
• Deflection
• Detection

16. MS/MS
SPECTROMETER
• Collision Induced Dissociation (CID)
• Electron-capture Dissociation (ECD)
• Electron Transfer Dissociation (ETD)
• Post Source Decay (PSD)
• Surface-induced Dissociation (SID)
Fig: Tandem mass spectrometer

17. HOW IT WORKS?

18. RESEARCH
Important Tool
INDUSTRY
Designing
Developing
Sales & Marketing