Production of Antibiotics for Microbiology & Pharmacy Students.

1. Production of Antibiotics
PRESENTED BY:-
Sheikh Mohammad Talha
BPH in RCH
Bangladesh University of Health Sciences(BUHS)
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2. Key Points
 Introduction
 What is Antibiotics?
 Application of Antibiotics.
 Types of Antibiotics.
 On the basis Mechanism of
Action.
 Production of Antibiotics.
 Steps in the production of
Penicillin.
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3. Introduction
1.Fleming (1929): First reported the lytic action of the
green mold (Penicillium Notatum) on staphylococci.He
called the culture fluid of the fungus containing
antibacterial substance as penicillin.
2.Florey and Chain (1940): Successfully isolated penicillin
compound,which however,prove to be low moleculer
substance rather than an enzyme.This pure replication
aroused the universal interest in this substance and it
saved millions of peoples life after the Word War II.
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4. What is Antibiotics?
Defition :
Substance (such as penicillin)
that destroy or inhabit the growth of
other pathogenic microorganism
and is used in the treatment of
external or internal infection.
OR,
While some antibiotics are
produce by microorganism, most
are now manufactured synthetically.
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5. Application of antibiotics
• Antibiotic or their modifications are indispensable medicinal
preparation.
• Antibiotic are used advantageously in agriculture and
poultry or animal
• Some antibiotic are used successfully to preserve perishable
foods.
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6. Class of Antibiotics
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7. Production of Antibiotics
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11. Industrial production techniques:
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Fermentation :
Industrial microbiology can be used to produce
antibiotic via the process of fermentation. Oxygen
concentration temperature, PH and nutrient level must
be optimal and are closely mentored and adjusted if
necessary. As antibiotic are secondary metabolites. Once
the process is complete the antibiotic must be extracted
and purified to a crystalline product.

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14. Batch-fed fermenter
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15. Strains used for the production
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In the raeliest year of antibiotics discovery the antibiotic
being discovered were naturally produced antibiotic and
were either produced by fungi, such as the antibiotic
penicillin or by soil bacteria, which car produced
antibiotic including Streptomycin and tetracycline.some
antibiotic are produced naturally by fungi .The include
the cephalosporin producing acremonium
chrysogenum.
Streptomycin is produced by stereptomyces griseus.
Tetracycline is produced by streptomyces aureofaciens.

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17. Isolation and Purification
• 3-5days, the maximum amount
of antibiotic will have been
produced
• The isolation process can begin.
• Depending on the specific
antibiotic produced, the
fermentation broth is processed
by various purification methods.
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18. Common organism used in antibiotic
 Bacillus subtilis.
 Bacillus polymyxa.
 Streptomyces nodosus.
 Streptomyces griseus.
 Penicillium notatum.
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