Snake venoms contain various proteins and enzymes that have potential medical applications. Some components can destroy tumor cells and may help treat cancers like lung cancer, breast cancer, and ovarian cancer. Different snakes' venoms contain different cytotoxic, cardiotoxic, or neurotoxic proteins that are being researched for their ability to selectively target and destroy cancer cells. While still in early research stages, certain snake venom components have shown promise in inhibiting cancer cell growth and metastasis, and may eventually lead to new natural treatments for various types of cancer.

1. Under the supervision of
Dr. Pracheta
Department Of Bioscience And Biotechnology,
Banasthali University

2. Introduction
 Snake are the most feared animal in the world.
 Snakes are elongated limbless, flexible and highly evolved reptiles
belonging to the phylum: Chordata,class: Reptilia, order: Squamata.
 Widely distributed throughoutthe world except in Antarctica, New Zealand
and Ireland.
 Common in tropical and sub tropical regions.
 Nearly 3500 species of snake found all over the world.

3. Aim and objective
Anticancerpotentialof snake venom it is a research about curing cancer by
snake venom.
Snake venom composition are important, becauseit determine which snakes
venom compound is cure diseases. All snakes venom composition are
different.
In anticancerpotentialof snake venom we found that, nowadays venom is
important in medical aspect.
This report highlight the importanceof snake venom in the management of
cancer.

4. Type of snakes
Venomous snake Non venomous snake
King cobra
Indian taipan Saw scaled viper
Many –banded
krait
Mulga snakeHorned
viper
Indian Python Boa constrictors Ball Python
Four-lined
Snake
Milk snake Bull
Snake

5. Different morphological features of venomous &
non venomous snakes
S. No. Venomous snake Non –venomous snake
1 Timber rattlesnakes (Crotatus horridus) (Wright,
Wright, 1957)
Recognized by a tail rattle, a shoved head
facial pits and vertical pupils.
Adult length 36-60 inches (Conant, 1975).
Typically a yellowish color with brown or black
bands down its back.
Eastern hognose (Heterodon platirhinos)
Distinguished by an upturned snout, lack of facial pits and
round pupils.
Adult length 18-45 inches (Smith and Brodie, 1982).
Females being larger than males.
Variable coloration; can be spoted yellow or all black.
2 Copperhead (Agkistrodon contortrix)
Recognized by a copper, brown, red shovel head,
facial pits and vertical pupils.
Adult length 20-37 inches.
Found in fields, forests or rocky outcrops.
Eastern milk snake (Lampropeltis triangulum)
Distinguished by a Y or V on the top of its head, a lack of
facial pits and rounded pupils.
Adult length 20-60 inches (Savitzky et al., 2004).
Found in fields, forests or rocky outcrops (Savitzky et al.,
2004).
3 Cottonmouth (Agkistrodon piscivorus)
Recognized by black ‘Hourglass’ bands on its
back, a flattened head and facial pit, vertical pupils.
Adult length 26-35 inches (Kardong, 1982).
Found near fresh water.
Northern water snake (Nerodia sipedon)
Distinguished by dark bands down its body, a narrow
head, a slender body, a lack of facial pits and round pupils.
Adult length 24-42 inches.
Found near fresh water.

6. Different characteristics features of venomous and non venomous snake
Differentmorphologicalfeatures of venomous & non venomous snakes

7. Venomous snakes family-
venomous snakes families are –Atractaspididae, Colubridae, Elapidae and Viperidae,
Capecentipedeeater
(Aparallactus capensis)
Spotted harlequin snake
(Homorosel-apslaceteus)
Southern stiletto snake
(Atractaspis bibronii)
Aesculapian snake
(Elaphe longissima)
American green snakes
(Opheodrys)
American garter snake
(Thamnophis)
Differenttype of venomous snakes belongs to family-Atractaspididae,
Colubridae.

8. Cobra (Ohiophagus hannah) Krait (Bungarus) Coral snake (Micrurus)Mambas (Dendroaspis)
European adders
(Vipera berus)
Gaboon vipers
(Bitis gabonica)
Carpet viper (Echis) Rusell’s viper (Daboia
russelii)
Rattle snake
(Crotalus)
Bushmasters (Lachesis) Asia pit viper
(Trimeresurus)
Malaya pit viper
(Calloselasma)
Different type of venomous snakes belongs to family-Elapidae, viperidae.

9. Snake venom
 Snake venom is highly modified saliva.
 Snake use their venom to immobilize, kill and digest the prey.
 It is a rich bio resource of biologicallyactive compound.
 It is yellow, green or colorlessliquid.
 A thick liquid composed of both organic and inorganic component of
enzymatic, non –enzymatic, protein, peptidesand toxins.

10. Composition of snake venom
Enzymes
Composition of snake venom
Proteins Organic compound Peptides
Phopholipase A2
Phosphodiesterase
Phosphomonoesterases
L-amino acid oxidase
Disintegrin
Ancrod
Citrate
Nucleosides
Bradykinin-
Potentiating Peptides
(BPP)
Protinases
Pyroglutamyl-
peptide
Collagenase

11. Enzymes found in snake venoms
Enzymes found in all venoms
Phospholipase A2
Deoxyribonuclease
Phosphodiesterase
Adenosine triphosphatase
Phosphomonoesterase
NAD nucleosidase
L-amino acid oxidase
Ribonuclease
5` Nucleotidase
Hyaluronidase
Enzymes found mainly in Elapid venoms
Acetylcholinesterase
Phospholipase B
Glycerophosphatase
Enzymes found mainly in Viperid
venoms
Endopeptidase
Kininogenase
Arginine ester hydrolase
Thrombin like enzyme
Factor X activator
Prothrmbin activator
Enzymes found in some snake venoms
Glutamate-pyruvate transaminase
Catalase
Amylase
Lactate dehydrogenase
Heparin like enzyme

12. Types of snake venom
Snake
Venom
Cytotoxin
Cardiotoxin
Neurotoxin
Hemotoxin
Destroy tumor cells
Treat breast cancer
Ovarian cancer
Cause the heart to
beat irregularly or
stop beating,
Causing death.
Attack nervous
system.
Used to treat brain
injuries, liver, lung
and skin cancer.
Destroy RBCs i.e.
cause hemolysis.
Treat heart attacks
and blood disorders.

13. Cancer
 Cancer is an abnormal growth of cells.
 It is a major burden of disease in all developed and developing
countries.
 Ten of million of people are diagnosed with cancer around the
world.
 More than 100 types of cancer like-lung cancer, kidney cancer,
breast cancer and ovary cancer.
 Symptoms of cancer depend on the type and location of the
cancer.

14. Anticancer potential of snake venom
 The propertiesthat make venom harmful are also what make it is so valued
for medicine.
 ClaudeBernad, father of physiology, was the first one to realize the
involvement of some componentsof snake venom in different therapeutic
potentials.
 Calmette and his Friends in 1993, reported that use of snake venom for the
treatment of cancer in laboratoryanimal.
 Cobra venom was used in 1930sfor treating cancer.

15. Type of
cancer
Snake Snake venom
component
Biological functions
lung cancer,
blood cancer
Rattle snake &
pit vipers
Hemotoxin Destroy blood cell, paralyze the
cancer cells.
Lung cancer
& skin
cancer
Egyptian cobra Neurotoxin Use to treat lung and skin
cancer
Breast
cancer &
ovary cancer
South
American
snake
Cytotoxin Destroy tumor cells
Therapeutic applications of snake venom

16. Type of
cancer
Snake Snake venom
component
Biological
functions
Ovary cancer Vipera labentina
turnica (blunt-nosed
viper)
Toxic enzymes-LAAO,
PLA2
Inhibit NF-KB & STAT3
by inhibition of P50 &P60
translocation into nucleus
Breast cancer &
Ovary cancer
Bothrops jararraca Thrombin like enzyme Stop metastasis of breast
cancer as well as ovarian
cancer as it can inhibit
tumor dissemination &
angiogenesis
Lung cancer &
Breast cancer
Agkistrodon
contortrix
(American
copperhead)
Disintegrin like-
contortrostatin
Inhibit the growth of the
tumor- it also slowed
angiogenesis, block the
migration cells
Melanoma Ophiophagus
hannah (King
cobra)
Eristostatin, L-amino acid
oxidase
Used to convince the
human body to attack
melanoma cells.

17. Snake venom use in making medicines
Cobratoxan's main ingredient is the male's cobra venom
with methyl salicylate and different essential oils
Relives muscle pain.
Snake venom Pain Killer Essential oil Menthol Pain
Relief Back/Neck/Shoulder Pain Plaster
used as massaging oil to ease acute pains and aches,
sprains etc only for inner pain don't apply on cut an open
wound.
“snake oil” medicines supposedly treat joint pain.
Medicine Treatments

18. Mizon S-Venom Wrinkle
Tox cream
Stem-cell and peptide anti
wrinkle cream
Snake venom peptide cream
Viper venomAktiv cream
Snake venom scrub
Face mask
Wrinkle freeze serum
Snake venom Hair shampoo
Snake- hair oil
Snake – booster oil
Different types of cosmetics prepared by snake venom

19. Conclusion & Future prospective
 Snake venom is a complex mixture of several proteins or peptides, derived
from snake venom could producepotentiallyhuge medical benefits for
mankind.
 It is used as an anticanceragent.
 The therapeuticuse of snake venom provides an overview of different use
of snake venom for different therapies. Above description clearly indicates
that different componentsof the snake venom are being used for clinical
trial and they can be used as a natural therapeuticagent against cancer.
 In view of this snake venom may open the doors for new era of medicines
and research for treatment of cancer. This in turn may bring hope, smile and
relief to several cancer patient in future.

20. Thank you