Microsatellite are the short nucleotide base pair repeats. They are also known as SSR/STR/MNR/VNTR. Microsatellite also detects heterozygous traits. Various applications are mentioned here. And all about Microsatellite.

1. Microsatellite
By PIYUSH SHARMA
Microsatellites or SSRs (Simple
Sequence Repeats, litt and luty
termed in1989) are one of the most
indicated techniques to study
polymorphism between DNA
sequences

2. MICROSATELLITE
• Short tenden repeated sequences of DNA .
• These sequences occurs in Non coding sequences.
• Microsatellite are used as genetic markers.
• Repeated polynucleotide sequences units are of
di- hexa nucleotide base pair.

3. MICROSATELLITE
• If these nucleotide base pair are more than 6 base
pairs and upto 25 base pairs than it is known are
MINISATELLITE.
• MINISATELLITE are also known as VNTR.
• VNTR ( vriable numbers of tenden repeates ).

4. TERMINOLOGY
• DNA MOTIF – Short Special Pattern Repeatation Of
Base Pair in Necleotide sequences.
• SSR – Simple Sequence Repeats.
• STR – Short Tenden Repeats.
• VNTR – Variable Numbers Of Tendem Repeats.
• SSLP – Simple Sequence Length Polymorphism.

7. MICROSATELLITE

8. MICROSATELLITE
• Rich in A/T
• Amplified by PCR
• 20 – 25% in Humans
• 40 – 45% in Plants
• CO–DOMINANT

9. CO DOMINANT

10. MOLECULAR MARKERS
• Microsatellite is CoDominant.
• In Heterozygote Allelic varation can be detected.
• Used as markers of genomic sequence.

11. MICROSATELLITE
ADVANTAGES :-
• Benefits of VERMICOMPOSTING :-
• Readily analysed by PCR.
• On Agarose Gel Electrophoresis it is observed
that there is large size different.
• Genotype output is high.

12. MICROSATELLITE
• Ancestry Analysis.
• Evaluation of Genetic Flow and Variation.
• Detection of Genetic Structure of Native plant
population.
• Application in Forensic science and Desease Diagnosis.

13. • REFERENCES
• Albertini E, Torricelli R, Bitocchi E, Raggi L, Marconi G, Pollastri L, Minco G, Battistini A, Papa R and Veronesi F (2011)
Structure of genetic diversity in Olea europaea L. cultivars from central Italy. Molecular Breeding 27: 533-547. [ Links ]
• Allard RW (1999) Principles of plant breeding. 2nded., J. Wiley & Sons, New York, 247p. [ Links ]
• Asfaw A, Blair M and Almekinders C (2009) Genetic diversity and population structure of common bean
(PhaseoREFERENCES
• Albertini E, Torricelli R, Bitocchi E, Raggi L, Marconi G, Pollastri L, Minco G, Battistini A, Papa R and Veronesi F (2011)
Structure of genetic diversity in Olea europaea L. cultivars from central Italy. Molecular Breeding 27: 533-547. [ Links ]
• Allard RW (1999) Principles of plant breeding. 2nded., J. Wiley & Sons, New York, 247p. [ Links ]
• Asfaw A, Blair M and Almekinders C (2009) Genetic diversity and population structure of common bean (Phaseolus
vulgaris L.) landraces from the East African highlands. Theoretical and Applied Genetics 120: 1-12. [ Links ]
• Benchimol LL, Souza Jr CL and Souza AP (2005) Microsatellite-assisted backcross selection in maize. Genetics and
Molecular Biology 28: 789-797. [ Links ]
• Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007)
Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins
using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762. [ Links ]
• lus vulgaris L.) landraces from the East African highlands. Theoretical and Applied Genetics 120: 1-12. [ Links ]
• Benchimol LL, Souza Jr CL and Souza AP (2005) Microsatellite-assisted backcross selection in maize. Genetics and
Molecular Biology 28: 789-797. [ Links ]
• Benchimol LL, Campos T, Carbonell SAM, Colombo CA, Chioratto AF, Formighieri EF, Gouvêa LRL and Souza AP (2007)
Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins
using new developed microsatellite markers. Genetic Resources and Crop Evolution 54: 1747-1762. [ Links ]