This document summarizes a research project on screening the phytochemicals and anti-inflammatory properties of Berginia ciliata (Dhungeful), a medicinal plant used in Pyuthan district of Nepal. The objectives are to identify phytochemicals present in Dhungeful extracts and test its anti-inflammatory activity in vitro and in vivo. Methods proposed include extracting rhizomes with methanol, ether and hexane, qualitative phytochemical analysis, and testing anti-inflammatory effects using HRBC membrane stabilization, lipid peroxidation inhibition, protein denaturation inhibition, and carrageenan-induced paw edema in rats. A timeline is provided and chemicals/materials estimated to cost 30740

1. SCREENING OF PHYTOCHEMICALS AND ANTI-
INFLAMMATORY PROPERTY OF BERGINIA CILIATA
(DHUNGEFUL)
Prepared by
Neeraj Ojha
Sonam Theeng
Yuvraj Kalathoki
BPHARM 7TH SEMESTER

2. INTRODUCTION
• Medicinal plants have been used for prevention or treatment of
different kinds of diseases since the Vedic periods dating back
to thousands of years .
• Medicinal plants are the major bio resource of drugs for both
traditional and conventional systems of medicine .
• Phytochemicals are the medicinally active compounds found
in plants; parts such as roots, barks, pulps etc.;
• These active compounds are responsible for showing
pharmacological activity .
• Many modern drugs were originally extracted from plant
sources even if they are now made synthetically & many other
drugs descend from plants;

3. Contd.
• The inflammatory response involves a complex array of
enzymes activation ,mediator release ,fluid extravasations, cell
migration , tissue breakdown and repair which are aimed at
host defense and usually activated in most disease condition ;
• In many inflammatory disorders there is excessive activation
of phagocytes, production of o2 and OH radicals as well as
non -free radical species which can harm the surrounding
tissue either by powerful direct oxidising action or indirectly
with hydrogen peroxide and OH radicals;
• Berginia ciliata commonly known as Dhungeful in
Pyuthan district is widely used to reduce inflammation mostly
backache and also as immune booster. The major parts used
are rhizome and leaves .Rhizomes are known to possess good
anti-inflammatory property ethno-medicinally;

4. Rationale of the Study
• Dhungeful is known to show better pain relieving property
ethno medically .As per the knowledge we have till now , no
study on anti-inflammatory property has been done on
the Dhungeful(Bergenia ciliata) that we have discovered in
mountainous areas of the Pyuthan district despite having
tremendous ethno-medicinal uses for this purpose.
• Moreover it has fast pain relieving property compared to other
medicinal plants .So ,if we are able to confirm the presence of
anti-inflammatory activity it will be of great importance;

5. Objective
• General objective:
Screening of phytochemicals and different biological property of
Dhungeful extract;
• Specific objective:
To screen the selected biological activity of dhungeful extract
(anti-inflammatory );
To identify the phytochemicals present;

6. Methodology
• Berginia ciliata rhizome will be extracted using different solvents
preferably methanol ,ether and hexane;
• The extract will be analysed qualitatively for phytochemicals
present in it.Preliminary clinical tests are going to be carried out for
the extract of Berginia ciliata to identify the presence of various
phytochemicals;
1. In -vitro anti inflammatory activity ;
• The human red blood cell (HRBC) membrane stabilization method;
• Lipid peroxidation study ;
• Protein denaturation study;
2. In -vivo anti inflammatory activity
• Carragenan induced rat paw edema;

7. S.No
.
Chemicals required Quantity Rate(in Rupees) Total Price (in
Rupees)
1. 1‐Diphenyl‐2‐Picrylhydrazyl (DPPH) 1 gm 10135 10135
2. Sodium nitroprusside 100 gms 2490 2490
3. Perchloric acid(60%) 500 gms 4360 4360
4. Ferrous sulphate 500 gms 400 400
5. Sodium nitrate Hi-AR” 500 gms 625 625
6. Bovine albumin fraction 100 ml 2920-14810 4000 approx.
7. Carragenan 1 kg 7730 7730
8. Aluminium Chloride/TBA/FCR Not
available
Not available Not available
9.
Animals (albino mice)
10 65-130 1000
Total Rs.30740/-

8. S.NO. ACTIVITY DEC/JAN FEB MAR APR MA
Y
JUN
1. LITERATURE REVIEW
2. TOPIC PRESENTATION
3. PROPOSAL DEFENCE
4. PROPOSAL SUBMISSION
5. PLANT
COLLECTN/IDENTIFICATION
6. MATERIALS
COLLECTION/LAB
WORK/PHYTOCHEMICAL
SCRNG.
7. FINAL PRESENTATION
8. REPORT SUBMISSION