More Related Content
Similar to Ramen Spectroscopy Poster
Similar to Ramen Spectroscopy Poster (20)
Ramen Spectroscopy Poster
- 1. TEMPLATE DESIGN © 2008
www.PosterPresentations.com
Morven Gannon - C12760661
DT710 - BSc in Medical Device Innovation
Department of Manufacturing Engineering
Dublin Institute of Technology
Introduction
References
Application 1
[1] Wachsmann-Hogiu et al. Current Opinion in Biotechnology (2009) 20: 63-73
[2] Ghita et al. EPJ Techniques and Instrumentation (2015) 2:6
[3] Hauser et al. Advanced Drug Delivery Reviews 89 (2015) 27:15
[4] Pascut et al. Biochimica et Biophysica Acta 1830 (BBA) (2013) 3517 – 3524
[5] http://www.eurostemcell.org/factsheet/mesenchymal-stem-cells-other-bone-
marrow-stem-cells 3/12/15
RAMEN SPECTROSCOPY APPLICATION IN STEM-CELL ENGINEERING
• Assessing differentiation in neural progenitor stem
cells that can generate neurons and glial cells:
• This research is dedicated to treatment of
Parkinson’s, Alzheimer’s, Strokes and the chronic
inflammatory and trauma they cause in the CNS
and PNS with the replacement of in-vivo neural
and glial cells [2]
Stem cell applications have enormous potential in
regenerative therapy and already provide
engineered cell tissue to repair diseased cells,
replace dead ones and build scaffold structure grafts
encouraged to build new tissue. A great deal of
further research is required to make stem cell
applications affordable and practical enough to
encourage everyday usage in the medical sector
[1]. It should be:
• Non-destructive (without the toxins of fluorescent
labelling)
• Repeatable (time relative)
Raman Microscopic Spectroscopy (RMS) provides a
chemical fingerprint of a molecular particle by
reading the scattered in-elastic photons from a
monochromatic laser light source.
• This is done in stem cell research with a Raman
spectrometer coupled with an inverted microscope
and is more relevant recently due to technological
improvements.
Figure 2: RMS results for Neural Progenitor Cells differentiation (A)
displaying the RNA content difference in the cytoplasm (B) [2]
Figure 1: Typical RMS configuration to monitor spatial changes over
time in live cultured stem cells [2]
Application 2
• Recording and interpreting over a timespan the
mineralisation of bone nodules in-vitro using
cultured mesenchymal stem cells (MSC)
• RMS can observe the proliferation and
differentiation over time to assess the growth of the
nodule forming osteoblasts [4]
Figure 3: Time course RMS of mesenchymal stem cells differentiation
into osteoblasts. The spike forming at 1000 cm-1 indicating the
growing levels of hydroxyapatite, the scaffold for osteocytes [4]
Application 3
• Refining the process of in-vitro differentiation of
cardiac heart tissue (cardiomyocytes – CM) grown
from human embryonic stem cells (EBS), and
potentially dictate the differentiation:
Figure 4: Time course embryonic bodies differentiating into cardiac muscle [2]
Brightfield
Imaging
Figure 3: Differentiation path from MSC to connective tissue[5]