Compare density gradient centrifugation, Magnet-activated cell sorting (MACS), and Fluorescence-activated cell sorting (FACS) in the isolation of pure stem cell populations from a heterogeneous suspension. Discuss the advantages and disadvantages of each technology as well as the emerging (recent) methods in stem cells separation.
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.
Sharon Bahia from the European Collection of Authenticated Cell Cultures of P...OECD Environment
The OECD Test Guidelines Programme is increasingly integrating innovative methodologies for the safety testing of chemicals into regulatory standards. Ensuring transparency, broad and and fair access to these innovative methods are important principles. Recently, the OECD published Guiding Principles for Good Licensing Practices of protected elements in Test Guidelines. This webinar explained those principles and showed how they can be useful in your work. If you are from the community of test developers, this webinar video recording is for you!
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ
Compare density gradient centrifugation, Magnet-activated cell sorting (MACS), and Fluorescence-activated cell sorting (FACS) in the isolation of pure stem cell populations from a heterogeneous suspension. Discuss the advantages and disadvantages of each technology as well as the emerging (recent) methods in stem cells separation.
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.
Sharon Bahia from the European Collection of Authenticated Cell Cultures of P...OECD Environment
The OECD Test Guidelines Programme is increasingly integrating innovative methodologies for the safety testing of chemicals into regulatory standards. Ensuring transparency, broad and and fair access to these innovative methods are important principles. Recently, the OECD published Guiding Principles for Good Licensing Practices of protected elements in Test Guidelines. This webinar explained those principles and showed how they can be useful in your work. If you are from the community of test developers, this webinar video recording is for you!
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ
Microfluidics and organ on a chip technology is an interdisciplinary field of medical and engineering. It will replace the current methods of testing efficacy of drug viz. cells in dishes test and animal testing.
Implementation of Medical Image Analysis using Image Processing TechniquesYogeshIJTSRD
Clinical imaging is playing a fundamental limit in assessment and patching of affliction and discovering tumors and finding of threatening cells in less than ideal stage. As a standard system for perceiving bone features, is minute pictures were used. These photos are secured by using small radiography, where it expected to reiterated, drawn out and work raised measure. This method cant recognize the destructive cells because of the presence of uproar in the photos. Hence there is a necessity for automated and strong strategies to finish the image planning examination. As a first stage, the most fundamental piece of picture planning is to denoising without barging in on the diagnostics information during the clearing of commotion. The past collaboration disposes of the uproar and present fog in the image. To get precise picture getting ready, we have executed fragile and hard breaking point with various coefficients and to check the edge Visu wither was used. It was found that the Wavelet deionsing gadget was a helpful resource for picture improvement. In the gathering, our proposed work was connected with pre planning methodology to wipe out the noise and to get smooth pictures. This collaboration will help with improving the idea of the image and besides take out the fake areas. To recognize the presence of bone illness and to choose its stage, K infers estimation was used and thusly to get smooth picture, edge division measure was performed. Miss. Kode Keerthi | Mr. Parasurama N "Implementation of Medical Image Analysis using Image Processing Techniques" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-3 , April 2021, URL: https://www.ijtsrd.com/papers/ijtsrd39893.pdf Paper URL: https://www.ijtsrd.com/engineering/electronics-and-communication-engineering/39893/implementation-of-medical-image-analysis-using-image-processing-techniques/miss-kode-keerthi
Plasmonic wave assessment via optomechatronics system for biosensor applicationIJECEIAES
Transduction biosensor (mass-based, optical and electrochemical) involves analysis, recognition and amplification in the acquired sample. In this work, the plasmonic-based biosensor was employed without using tags. It is crucial to determine angles of Brewster (Ɵb) and critical (Ɵc) for generating plasmonic resonance (Ɵr). The objective is to verify a cost-effective plasmonic biosensor through Fresnel simulation and experimentation of a developed optomechatronics system. The borosilicate glass, Au and Air layers were simulated with the Winspall 3.02 simulator. The optomechatronics system consists of: 1-optics (650 nm laser, slit, polarizer, photodiode), 2-mechanical (bipolar stepper motors, gears, stages) and 3-electronics (PIC18F4550, liquid crystal display (LCD) and drivers). Later, the software performs angular interrogation by reading the reflected beam from a rotating prism at 0.1125. Experimentation to simulation accuracy indicates that percentage differences for Ɵr and Ɵc are 1% and 0.2%, respectively. In conclusion, excellence verification was successfully achieved between experimentation and simulation. It proved that the lowcost optomechatronics system is capable and reliable to be deployed for the biosensor application.
brief introduction on bone structure and common bone disease, mechanism of targeting, structure and composition of bone delivery system with mentioning some gaps in current research
Different kidney diseases affect different part of kidney. For example, kidney tumor usually occurs in renal cortex, renal column hypertrophy may exist in renal column, medullary cystic kidney disease usually exists in renal medulla, and transitional cell cancer, renal pelvis and ureter cancer may attack renal pelvis To propose automatically segment kidney and detect disease occurs in kidney.Random forest and modified active appearance model can performed in this project.
Neutron Imaging and Tomography with Medipix2 and Dental Microroentgenography:...IJAEMSJORNAL
An over view of Neutron Imaging and Tomography (NIT) with Medipix2 and Dental Micro-roentgenography have been presented in this article. This over view confined to semiconductor detector Medipix2, neutron radiography and tomography and dental microroentgenography. Medipix2 is a pixel-based detector technology employed to measure charge particles, photons (visible through gammas) and neutron. Neutron Beam for this technology are LVR-15 Research Reactor ( 107 n/cm2 s) and Spallation neutron source ( 3×106n/cm2 s) .This technology has been verified with photograph and neutronogram of a relay and photograph and tomographic 3D reconstruction of a bullet cartidge, tooth and fishing thread. Comparison of spatial resolution among different imagers also has been presented.
1. Flowcytometery : Principles and applications.
2. Hemocytometery: Principles and applications.
3. Chromatography: Types, Principles and application
4. Electrophoresis: Types, Principles and application
1. PCR and transillumnator: Theory and its applications to biomedical field.
2. Inoculation and isolation of Microorganism from the different type of samples.
Microfluidics and organ on a chip technology is an interdisciplinary field of medical and engineering. It will replace the current methods of testing efficacy of drug viz. cells in dishes test and animal testing.
Implementation of Medical Image Analysis using Image Processing TechniquesYogeshIJTSRD
Clinical imaging is playing a fundamental limit in assessment and patching of affliction and discovering tumors and finding of threatening cells in less than ideal stage. As a standard system for perceiving bone features, is minute pictures were used. These photos are secured by using small radiography, where it expected to reiterated, drawn out and work raised measure. This method cant recognize the destructive cells because of the presence of uproar in the photos. Hence there is a necessity for automated and strong strategies to finish the image planning examination. As a first stage, the most fundamental piece of picture planning is to denoising without barging in on the diagnostics information during the clearing of commotion. The past collaboration disposes of the uproar and present fog in the image. To get precise picture getting ready, we have executed fragile and hard breaking point with various coefficients and to check the edge Visu wither was used. It was found that the Wavelet deionsing gadget was a helpful resource for picture improvement. In the gathering, our proposed work was connected with pre planning methodology to wipe out the noise and to get smooth pictures. This collaboration will help with improving the idea of the image and besides take out the fake areas. To recognize the presence of bone illness and to choose its stage, K infers estimation was used and thusly to get smooth picture, edge division measure was performed. Miss. Kode Keerthi | Mr. Parasurama N "Implementation of Medical Image Analysis using Image Processing Techniques" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-3 , April 2021, URL: https://www.ijtsrd.com/papers/ijtsrd39893.pdf Paper URL: https://www.ijtsrd.com/engineering/electronics-and-communication-engineering/39893/implementation-of-medical-image-analysis-using-image-processing-techniques/miss-kode-keerthi
Plasmonic wave assessment via optomechatronics system for biosensor applicationIJECEIAES
Transduction biosensor (mass-based, optical and electrochemical) involves analysis, recognition and amplification in the acquired sample. In this work, the plasmonic-based biosensor was employed without using tags. It is crucial to determine angles of Brewster (Ɵb) and critical (Ɵc) for generating plasmonic resonance (Ɵr). The objective is to verify a cost-effective plasmonic biosensor through Fresnel simulation and experimentation of a developed optomechatronics system. The borosilicate glass, Au and Air layers were simulated with the Winspall 3.02 simulator. The optomechatronics system consists of: 1-optics (650 nm laser, slit, polarizer, photodiode), 2-mechanical (bipolar stepper motors, gears, stages) and 3-electronics (PIC18F4550, liquid crystal display (LCD) and drivers). Later, the software performs angular interrogation by reading the reflected beam from a rotating prism at 0.1125. Experimentation to simulation accuracy indicates that percentage differences for Ɵr and Ɵc are 1% and 0.2%, respectively. In conclusion, excellence verification was successfully achieved between experimentation and simulation. It proved that the lowcost optomechatronics system is capable and reliable to be deployed for the biosensor application.
brief introduction on bone structure and common bone disease, mechanism of targeting, structure and composition of bone delivery system with mentioning some gaps in current research
Different kidney diseases affect different part of kidney. For example, kidney tumor usually occurs in renal cortex, renal column hypertrophy may exist in renal column, medullary cystic kidney disease usually exists in renal medulla, and transitional cell cancer, renal pelvis and ureter cancer may attack renal pelvis To propose automatically segment kidney and detect disease occurs in kidney.Random forest and modified active appearance model can performed in this project.
Neutron Imaging and Tomography with Medipix2 and Dental Microroentgenography:...IJAEMSJORNAL
An over view of Neutron Imaging and Tomography (NIT) with Medipix2 and Dental Micro-roentgenography have been presented in this article. This over view confined to semiconductor detector Medipix2, neutron radiography and tomography and dental microroentgenography. Medipix2 is a pixel-based detector technology employed to measure charge particles, photons (visible through gammas) and neutron. Neutron Beam for this technology are LVR-15 Research Reactor ( 107 n/cm2 s) and Spallation neutron source ( 3×106n/cm2 s) .This technology has been verified with photograph and neutronogram of a relay and photograph and tomographic 3D reconstruction of a bullet cartidge, tooth and fishing thread. Comparison of spatial resolution among different imagers also has been presented.
1. Flowcytometery : Principles and applications.
2. Hemocytometery: Principles and applications.
3. Chromatography: Types, Principles and application
4. Electrophoresis: Types, Principles and application
1. PCR and transillumnator: Theory and its applications to biomedical field.
2. Inoculation and isolation of Microorganism from the different type of samples.
Optical Coherence Tomography: Technology and applications for neuroimagingManish Kumar
Optical coherence tomography (OCT) is an emerging imaging technology with applications in biology, medicine, and materials investigations. Attractive features include high cellular-level resolution, real-time acquisition rates, and spectroscopic feature extraction in a compact noninvasive instrument. OCT can perform ‘‘optical biopsies’’ of tissue, producing images approaching the resolution of histology without having to resect and histologically process tissue specimens for characterization and diagnosis.
Advances and Innovations and Impediments in Tissue Engineering and Regenerati...CrimsonpublishersITERM
The evolving arena of regenerative medicine requires the use of cells, scaffolds, tissues or genetically edited elements as therapeutic agents for implantable engineered tissues and organs that can regenerate physiological functions. A variety of fabrication techniques like gas foaming, phase separation, salt leaching, and freeze drying have been developed that successfully regenerate complex and functional tissues. Recently developed three-dimensional (3D) printing technology promises to bridge the differences between artificially engineered tissues and native tissues. After 3D printing, 3D bioprinting was introduced as an ultimate solution for vascularized tissue fabrication. The large number of tissues such as bone, cartilage, skin, myocardial, kidney, liver, and lung tissue models were investigated with 3D bioprinting. As there is a need for stimulus-responsive geometry, four-dimensional (4D) printing technology has been developed to fabricate structures that can transform their shape. Tissue engineering and regenerative medicine providing exciting results has brought a new era of medical research and applications with value addition in the field of medicine. It will be important to ensure that appropriate technologies are developed, validated that could result to the betterment of human situation. The positive role of regulatory authorities could enhance the morale of the researchers and scientific communities. This could translate in to the lifesaving new innovations.