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- 1. Cell Authentication By STR Profiling
- 2. Survey responses: Quality control of cultured cells/cell lines. (Click to enlarge) Cell Line Culture Survey Results
- 4. Require Cell Line Authentication Nature Publishing Group Nature Reviews Molecular Cell Biology Nature Nature Genetics Nature Reviews Immunology Nature Reviews Cancer Nature Reviews Neuroscience Nature Biotechnology Nature Methods Society for Endocrinology Journals Journal of Endocrinology Journal of Molecular Endocrinology Endocrine-Related Cancer AACR Journals Cancer Discovery Cancer Research Clinical Cancer Research Cancer Epidemiology, Biomarkers & Prevention Molecular Cancer Research Molecular Cancer Therapeutics Cancer Prevention Research Endocrine Society Journals Endocrinology Endocrine Reviews Journal of Clinical Endocrinology & Metabolism Molecular Endocrinology Hormones and Cancer BioTechniques Journals that Require Cell Line Authentication The following is a list of just some of many peer-review journals that are known to require cell line authentication for all submitted research involving cell lines.
- 5. Failure to monitor cell lines for cross-contamination or misidentification, a problem that has persisted for cell lines for decades, may result in compromised data and ultimately irreproducible results. How to solve this problem? A Misidentification due to labeling errors B Incorrect classification by pathologists C Cross-contamination with other cell types
- 6. Intruduction What is Short tandem repeat? Short tandem repeat is a microsatellite, consisting of a unit of two to thirteen nucleotides repeated hundreds of times in a row on the DNA strand. Standards for Cell Line Authentication short tandem repeat [STR] profiling
- 7. DNA Polymorphisms Most of our DNA is identical to DNA of others. However, there are inherited regions of our DNA that can vary from person to person. Variations in DNA sequence between individuals are termed "polymorphisms". STR STRs are short sequences of DNA, normally of length 2-5 base pairs, that are repeated numerous times in a head-tail manner. These Sequences with the highest degree of polymorphism are very useful for DNA analysis in forensics cases and paternity testing. STR Background
- 8. 13 Core STR Loci
- 10. DNA Extraction Multiplex PCR STR allele seperation And Sizing STR profile comparison analysis Bioinformati cs Database Analysis Complete STR profile report by request STR Profiling Workflow
- 11. 1 2 3 4 5 6 Complete STR profile report by request STR profile comparison analysis DNA extraction and amplification included Cross contamination detection and analysis Fast turnaround time High Accuracy, Competitive Price Highlight
- 12. P h o n e : 1 - 6 3 1 - 6 2 6 - 9 1 8 1 F a x : 1 - 6 3 1 - 6 1 4 - 7 8 2 8 A d d r e s s : 4 5 - 1 R a m s e y R o a d , S h i r l e y , N Y 1 1 9 6 7 , U S A E m a i l : i n f o @ c r e a t i v e - b i o a r r a y . c o m
Editor's Notes
- STR DNA profiling is now a powerful, inexpensive tool that can generate unique DNA signatures that can be used to authenticate cell lines and detect contamination of more than one cell type. This presentation will talk about why scientists need cell authentication, what is STR profile and STR profile workflow from Creative Bioarray.
- Accurate documentation of cell line tissue of origin, sex, and species are critical to ensure the credibility, reproducibility, and translation of data and results from cell culture-based experiments. The financial implications of misidentified or contaminated cell lines can be profound; as much as $700 million dollars per year in research that could be at risk. A Survey conducted by GBSI shows that The majority (52%) of respondents never perform authentication or other species-related QC tests on cell lines used in their experiments. Moreover, 74% never conduct STR profiling.
- When it comes to training, only 62% of respondents (Figure 2) had received specific training on the problems of cell line misidentification (cross-contamination) and mislabeling, and less than one-third (30%) were trained on the importance of cell line authentication as a quality control measure for species confirmation. Furthermore, only 65% of respondents indicated that their laboratory follows specific written procedures for the handling and storage of cultured cells and cell lines.
- Many Journals Require Cell Line Authentication. The following is a list of just some of many peer-review journals that are known to require cell line authentication for all submitted research involving cell lines, like AACR journals group, nature publishing group.
- Failure to monitor cell lines for cross-contamination or misidentification, a problem that has persisted for cell lines for decades, may result in compromised data and ultimately irreproducible results. How to solve this problem? .
- Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat [STR] profiling) has been the subject of a comprehensive and definitive standard. An international team of scientists is preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. So what is STR profiling? Short tandem repeat is a microsatellite, consisting of a unit of two to thirteen nucleotides repeated hundreds of times in a row on the DNA strand.
- Most of our DNA is identical to DNA of others. However, there are inherited regions of our DNA that can vary from person to person. Variations in DNA sequence between individuals are termed "polymorphisms". STRs are short sequences of DNA, normally of length 2-5 base pairs, that are repeated numerous times in a head-tail manner. These Sequences with the highest degree of polymorphism are very useful for DNA analysis in forensics cases and paternity testing.
- Although the human genome contains thousands upon thousands of STR markers, only a small core set of loci have been selected for use in forensic DNA and human identity testing . The 13 core STR loci used for the U.S. national DNA database are shown in the chart.
- The number of repeats of each STR at each genetic site varies within the human populations. STR profiling is the mehod to identify individuals or cell lines by characteristics of their DNA.
- Creative Bioarray offers STR analysis service. We offers unique customized solutions to fit any need. Our STR profiling workflow is from the DNA extraction to str profile report result.If you have an immediate need or further questions, please contact Creative Bioarray at info@creative-bioarray.com or 1-631-626-9181.
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