A research report published in the international magazine Genes & Development, scientists from the Children's Hospital of Philadelphia used a powerful new technology to RNA sequence 20,000 single nuclei, opening up the biological events behind heart disease. https://www.creative-bioarray.com/services/stem-cell-research.htm

1. Creative Bioarray
The Complete Guide to Know That RNA Sequencing of Cardiac Cells May
Elucidate Cardiac Developmental Mechanisms and Develop New Cardiac
Therapies

2. CONTENTS
01 Brief Introduction
02 Process of Research
03 Application
04 Conclusion

3. Brief Introduction
Report publishing biological events
animal study transcription blueprint

4. Brief Introduction
Recently, a research report published in the
international magazine Genes & Development,
scientists from the Children's Hospital of
Philadelphia used a powerful new technology
to RNA sequence 20,000 single nuclei, opening
up the biological events behind heart disease.
In animal studies, researchers identified multiple
cell types in healthy and diseased hearts, and
researchers also studied the ‘transcription
blueprint’ in detail, where DNA transcribes
genetic information into RNA and protein.

5. Brief Introduction (II)
The researchers claimed that they applied a large number of parallel single-nuclear RNA
sequencing technologies to heart studies in postnatal mice for the first time, which may provide
biological events involved in normal heart development and heart disease. Their final goal is to
find targeted therapies for heart disease, in addition, this type of large-scale sequencing can be
applied to many medical fields.

6. Process of Experiment
scRNA-seq central nervous system
sNucDrop-seq gene regulation

7. scRNA-seq
In the past three years, researchers have begun
to use a large number of parallel single-cell RNA
sequencing technologies (scRNA-seq), however,
due to the large size of cardiomyocytes,
researchers face many challenges in studying
the technology of single cells in the heart after
birth.

8. Central Nervous System
01
In order to enable
analysis of large cells
(such as muscle cells)
or complex
morphological cells
(such as neuronal
cells)
0202
researchers have
developed a number
of parallel single-
nuclear sequencing
(snRNA-seq)
technologies
0303
but so far, this
technique can only
be used to study the
central nervous
system
0404
In this study,
researchers first tried to
use the snRNA-seq
technology to study the
heart tissue of an
individual after birth.

9. sNucDrop-seq
The researchers analyzed 20,000 nuclei from
heart tissue from normal and diseased mice
with a snRNA-seq method called sNucDrop-seq,
and they have developed the sNucDrop-seq
technology and applied it to the heart of a
mammal's birth. At present, researchers only
pay attention to the study of cardiomyopathy.
The main manifestation of cardiomyopathy is
the progressive degeneration of heart tissue,
which is also the common cause of heart failure
in individuals.

10. Gene regulation
Researchers use mice to mimic childhood
mitochondrial cardiomyopathy. The
researchers say the heart is a very complex
organ that contains multiple cell types
In this study, the researchers focused
on three areas of research
researchers are still unclear about the
mechanisms of heart development and
heart disease in mammals after birth.
normal heart development, heart disease,
and the gene regulation mechanism of the
cardiac hormone GDF15.

11. Application
Sequencing tools Metabolic changes
GDF15 Targeted therapies

12. Sequencing tools
They use sequencing tools to identify
cells such as cardiomyocytes, fibroblasts,
and endothelial cells.
The researchers found that each cell type is
very different, in the case of normal disease,
there will be signs of functional changes in the
heart cells
researchers can detect metabolic changes
in fibroblasts

13. GDF15
In addition, the researchers also found a network of
genes that regulate heart hormones (especially GDF15)
during heart disease. Cardiac hormones can slow down
overall body growth, presumably to reduce the energy
requirements of the damaged heart.
Such signals may shed light on the more biological
mechanisms behind the growth limitation of children
with congenital heart disease. Researchers prove that in-
depth heart biology research can help researchers
develop targeted therapies to work with key genetic
networks, and it is expected to develop more therapies
for patients with heart disease.

14. Conclusion

15. Conclusion
This study is the first step in the study of researchers to define the
"transcriptional landscape" of normal and diseased hearts at high
resolution. Furthermore, researchers are expected to clarify the
progression of heart disease with age through more in-depth research,
and the tools developed by researchers can help to develop organs or
systems outside the heart.

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