Presentation of Edible Vaccine, Properties of an edible vaccine, how to make an edible vaccine, and etc. An edible vaccine is the best vaccine for developing country because of it very cost effective and very simple to use. But our country has not developed this vaccine yet. So it is our responsibility to develop an edible vaccine to protect our human being form more infectious disease.

1. Presentation On
Plant Genetic Engineering Practical(BGE3206)

2. Submitted By
ASH1413061M | ASH1413062M
ASH1413064M | ASH1413065M
BKH1413071F
Submitted To
Md. Anwar Hossain |Assistant Professor
Razia Sultana | Assistant Professor
Asma Talukder | Lecturer

3. Vaccine
Vaccine is an antigenic substance prepared from the causative agent of a disease or a synthetic
substitute, used to provide immunity against one or several disease.
Smallpox vaccine, the first successful vaccine was introduced by Edward Jenner in 1796.
Edible Vaccine
A vaccine in which an antigenic substance is engineered into an edible part of a plant.
The concept of edible vaccine was developed by Arntzen in the 1990s.

4.  Should not be toxic
 Low levels of side effect
 Should not contaminate the environment
 Should not cause problem in individual
 Technique of vaccination should be simple
 Should be cheap
 Should provide long lasting immunity

5. Recombinant
Protein(vaccine)
Transgenic plant Protection against
Rabies glycoprotein Tomato Rabies virus
Foot & mouth virus Arabidopsis Foot & mouth virus
Herpes virus B surface antigen Tobacco Herpes simplex virus
Cholera toxin B subunit Potato Vibrio cholerae
Human cytomegalovirus
glycoprotein B
Tobacco Human cytomegalovirus

6. Traditional vaccine Edible vaccine
Too expensive to be used on large scale Comparatively less expensive
Lack of physical infrastructure makes it
impossible to disseminate the vaccine
May be easily available
Required trained personnel to administer
injections
Do not require any trained personnel to
administer
Required elaborate production facilities,
purification, sterilization, packaging
No purification strategies required
Can’t directly stimulate the immune system Vaccine when taken orally, can directly
stimulate the immune system

10.  Cost effective
 Easy to administer
 Easy to store
 Acceptable to poor developing countries
 Active both mucosal & systemic immunity
 Heat stable
 Do not require cold chain maintenance
 No fear of contamination
 Delivery of multiple antigens

11.  Transgenic contamination can occur.
 Antibiotic resistance marker gene can spread from GM food to
pathogenic bacteria.
 Selection of best plant is difficult.
 Difficulty in dose maintenance.
 Consistency of dosage from fruit to fruit, plant to plant &
generation to generation is not similar.
 Stability of vaccine in fruit is not known.

12.  The first trial on humans in 1997 (using the heat labile B- toxin from E. coli) is a milestone
on the road to creating inexpensive vaccines that might be particularly useful in
immunizing people in developing countries, where high cost and logistical issues, such as
transportation and the need for certain vaccines to be refrigerated, can thwart effective
vaccination programs.
 The hope is that edible vaccines could be grown in many of the developing countries
where they would actually be used.
 Whatever may be the current situation, a day is not far off when we will be able to pluck a
fruit from the garden, eat it and be protected from diseases…making needles needless…