4. Enzyme & Substrates :
Enzyme: A substance produced by a living organism that acts as a catalyst to bring
about a specific biochemical reaction.
Substrates: In biochemistry, the substrate is a molecule upon which an enzyme acts.
Enzymes catalyze chemical reactions involving the substrate(s).
Enzyme are highly specific in their active site.
Enzymes and substrates are bind together in a model called “lock and key”.
5. Mechanism of enzyme action:
The substrate molecule bind it self to the active sites on the surface of enzyme. The
active site in a given enzyme is so shaped that only a specific substrate can fit in it. Just
like a lock and key. The specific binding results in the formation of enzyme substrate
complex.
Enzyme lower down the activation energy of reaction.
6. Example:
Activation energy of acid hydrolysis of sucrose is 6.22 kj/mol. But activation
energy in only 2.15 kj/mol. When hydrolysis take place in presence of sucrose
enzyme.
7. Cont.…
At finally product are produce.
The active site of the enzyme change shape and can no longer bind to
the substrate. It been denatured.