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NAME : BRENDA CHONG (00011203)
LEE ENG SHENG (00011155)
INSTRUCTOR : MS. PUTRI SHAREEN
Tuberculosis is caused by the infectious agent
known as Mycobacterium tuberculosis (Mtb).
Koch's bacillus.
Gram-resistant (waxy cell walls)
Non motile
Pleomorphic rods
Pathogenic *to humans
1. Small, slow-growing bacterium that can live
only in human body.
2. Aerobic bacterium, needs oxygen to survive.
3. During active TB disease, Mtb complexes are
always found in the upper air sacs of the
lungs.
Acid-fast bacilli
The lipid-rich cell
wall s are
impermeable to
various basic dyes
(except for phenol
compounds)
1. Heat fix cells on glass microscope slide.
2. Flood the slide with carbol fuchsin stain.
3. Heat the slide gently until it steams (5 min).
4. Pour off the carbol fuchsin.
5. Wash slide thoroughly with water.
6. Decolourize with acid-alcohol (5 min).
7. Wash slide thoroughly with water.
8. Flood slide with methylene blue counterstain for 1 min.
9. Wash with water.
10. Blot excess water and dry in hand over bunsen flame.
It is spread through the air
from person to person
When TB patients cough, sneeze, spit, laugh or
talk.
The inhaled TB bacteria reach the lungs when
a person breathes in Mtb-contaminated air.
Latent TB infection. The bacteria can remain
dormant (asleep) for years and not cause any
TB disease.
The first effective
treatment for TB
was developed in
the 1940s –
streptomycin.
Infants often are vaccinated with bacille
Calmette-Guerin BCG injections.
(Home)
Stay home.
Ventilate the room.
Cover your mouth.
Wear a mask.
1. Mycobacterium Tuberculosis [Online]. Retrieved from :
http://www.microbiologybytes.com/video/Mtuberculosis.ht
ml [Accessed 29 JULY 2014]
2. http://www.mayoclinic.org/diseases-
conditions/tuberculosis/basics/prevention/con-20021761
[Accessed 22 JULY 2014]
3. http://www.niaid.nih.gov/topics/tuberculosis/understanding/
pages/cause.aspx [Accessed 22 JULY 2014]

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Tuberculosis

  • 1. NAME : BRENDA CHONG (00011203) LEE ENG SHENG (00011155) INSTRUCTOR : MS. PUTRI SHAREEN
  • 2. Tuberculosis is caused by the infectious agent known as Mycobacterium tuberculosis (Mtb). Koch's bacillus.
  • 3. Gram-resistant (waxy cell walls) Non motile Pleomorphic rods Pathogenic *to humans
  • 4. 1. Small, slow-growing bacterium that can live only in human body. 2. Aerobic bacterium, needs oxygen to survive. 3. During active TB disease, Mtb complexes are always found in the upper air sacs of the lungs.
  • 5. Acid-fast bacilli The lipid-rich cell wall s are impermeable to various basic dyes (except for phenol compounds)
  • 6. 1. Heat fix cells on glass microscope slide. 2. Flood the slide with carbol fuchsin stain. 3. Heat the slide gently until it steams (5 min). 4. Pour off the carbol fuchsin. 5. Wash slide thoroughly with water. 6. Decolourize with acid-alcohol (5 min). 7. Wash slide thoroughly with water. 8. Flood slide with methylene blue counterstain for 1 min. 9. Wash with water. 10. Blot excess water and dry in hand over bunsen flame.
  • 7.
  • 8. It is spread through the air from person to person When TB patients cough, sneeze, spit, laugh or talk.
  • 9. The inhaled TB bacteria reach the lungs when a person breathes in Mtb-contaminated air. Latent TB infection. The bacteria can remain dormant (asleep) for years and not cause any TB disease.
  • 10. The first effective treatment for TB was developed in the 1940s – streptomycin.
  • 11. Infants often are vaccinated with bacille Calmette-Guerin BCG injections. (Home) Stay home. Ventilate the room. Cover your mouth. Wear a mask.
  • 12. 1. Mycobacterium Tuberculosis [Online]. Retrieved from : http://www.microbiologybytes.com/video/Mtuberculosis.ht ml [Accessed 29 JULY 2014] 2. http://www.mayoclinic.org/diseases- conditions/tuberculosis/basics/prevention/con-20021761 [Accessed 22 JULY 2014] 3. http://www.niaid.nih.gov/topics/tuberculosis/understanding/ pages/cause.aspx [Accessed 22 JULY 2014]