Liposomes are highly biocompatible and easy to control in vivo. Due to their unique physicochemical characteristics, liposomes can incorporate hydrophilic, lipophilic, and hydrophobic therapeutic agents, including a broad variety of drugs, DNA, and diagnostic agents. Hydrophilic compounds may either be entrapped into the aqueous core of the liposomes or located at the interface between the lipid bilayer and the external water phase. Lipophilic or hydrophobic drugs are generally entrapped almost completely in the hydrophobic core of the lipid bilayers of the liposomes. https://www.creative-biolabs.com/lipid-based-delivery/

1. GET INSIDE
THE BRAIN!
Liposomes for brain drug delivery
Expert in Lipid Based Drug Delivery Services
Creative Biolabs|Lipid-based Drug Delivery

2. Contents
• Background
• Pathways for crossing the
blood-brain barrier (BBB)
• Liposome-based strategies
across the BBB
• Creative Biolabs’ liposome
development services
Liposome Development Services
Creative Biolabs has more than ten years of
experience in liposomal formulation
development. We integrate the knowledge of
well-known liposome academics into our
liposome technology. Our team of liposome
experts and support staff has successfully
served thousands of customers all over the
world. At Creative Biolabs, we provide a full
set of liposomes custom services and offer
various liposome analysis services to
characterize liposome-based products and
measure the embedding efficiency. We
believe our liposome products and services
will meet the highest standards.
Creative Biolabs|Lipid-based Drug Delivery

3. The blood–brain barrier (BBB) is a highly complex multicellular
structure that protects the brain from harmful substances and
invading organisms from the bloodstream, supplying brain tissue
with nutrients and controlling its homeostasis. The BBB is
composed of polarized endothelial cells connected by tight
junctions of the cerebral capillary endothelium and a variety of
transporters, which are responsible for its extremely low
permeability, limiting the delivery of drugs to the central nervous
system (CNS). BBB functionality is dynamically regulated by an
ensemble of different cell types, such as astrocytes, pericytes, and
neurons. Endothelial cells are surrounded by a basal lamina,
which is generally rich in laminin, fibronectin, type IV collagen,
and heparin sulfate, representing an interesting target for drug
transport and providing a negatively charged interface.
The development of new strategies to treat brain diseases is
challenging, considering the low drug accessibility to the brain
due to the presence of the BBB. In several high incidence brain
pathologies, such as stroke, brain infections, Alzheimer’s disease
(AD), and Parkinson’s disease (PD), the BBB is altered. Specialized
BBB components are more permeable if failed to maintain,
allowing the molecules that cause neuroinflammation and
neurodegeneration to enter the brain. However, even when
damaged and more permeable, the BBB can still be difficult for
drug delivery into the brain.
Background
Creative Biolabs|Lipid-based Drug Delivery
Fig 1. Cross-section of a cerebral capillary

4. CMT
AET
RMT
• Carrier Mediated Transport
• carbohydrates, fatty acids, amino acids, nucleotides,
hormones, vitamins, organic cations and anions
• Active Efflux Transport
• Receptor Mediated Transport
• peptides and proteins
Fig 2. Different drug delivery mechanisms across the BBB
Creative Biolabs|Lipid-based Drug Delivery

5. Liposome-based strategies across the BBB
Liposomes are a type of nano-sized phospholipid bubbles that have
attracted much attention as potential drug carriers. Liposomes are highly
biocompatible and easy to control in vivo. Due to their unique
physicochemical characteristics, liposomes can incorporate hydrophilic,
lipophilic, and hydrophobic therapeutic agents, including a broad variety of
drugs, DNA, and diagnostic agents. Hydrophilic compounds may either be
entrapped into the aqueous core of the liposomes or located at the interface
between the lipid bilayer and the external water phase. Lipophilic or
hydrophobic drugs are generally entrapped almost completely in the
hydrophobic core of the lipid bilayers of the liposomes.
Advantages of Liposome:
• Nano- or microsize vesicles
• Able to incorporate hydrophilic, lipophilic, and hydrophobic therapeutic
agents.
• Good biocompatibility and biodegradability, low toxicity, drug-targeted
delivery, and controlled drug release.
Creative Biolabs|Lipid-based Drug Delivery
Fig.3 Structure of liposomes.
(Débora B Vieira, 2016)

6. Liposomes are used to treat:
• Alzheimer’s Disease
• Parkinson’s Disease
• Stroke
• Glioma
Administration Routes:
• Intravenous injection
• Intraperitoneal injection
• Oral administration
• Intranasal administration
Creative Biolabs|Lipid-based Drug Delivery
Cationic Liposome Ligand-targeted liposome Stimuli-Responsive Liposome

7. Creative Biolabs’ liposome development and analysis services
Creative Biolabs|Lipid-based Drug Delivery

8. As a liposome contract manufacturer, Creative Biolabs is making high-end
liposomal products for our customers. We can design
and produce a liposomal product that is fully tailored to
your requirements. Our groundbreaking
research, objectified working process, and state-of-the-art
equipment mean that we can develop distinctive
liposomal formulations and products. Our customized liposome
services offer genuine phospholipid bi-layered
vesicles that contain the active substance. We can
produce liposomal formulations for any active
substance. Besides, our services make every
liposome unique and are subject
to stringent quality control. We guarantee that our
liposomes retain their quality and efficacy
during storage. For more details about our
services, please contact us.
Our custom liposome services can regulate the
solubility of encapsulated small organic molecules,
protect the embedded agents from degradation, and
achieve site-specific delivery. Our services include
liposome formulation design, liposome encapsulation,
and liposome-drug complex analysis and
characterization. We provide various novel liposome
formulations including immunoliposomes,
proteoliposomes, magnetic liposomes, fluorescent
liposomes, and pH-sensitive liposomes.
Features of Our Custom Liposome Services:
• Non-toxicity, biocompatible, and completely biodegradable
• Increased drug efficacy
• Site avoidance effect
• Increased stability
• Reduced drug toxicity
• Flexibility for active targeting
Creative Biolabs|Lipid-based Drug Delivery

9. CONTACT
SUITE203, 17RamseyRoad,Shirley, NY 11967, USA
Tel: 1-631-871-5806 (USA)
44-207-097-1828 (Europe)
Fax: 1-631-207-8356
Email: info@creative-biolabs.com
Creative Biolabs|Lipid-based Drug Delivery