pus sample,urine culture, and antibiotic susceptibility

1. Made by: Komal Mudgal ( section-A)
Enroll. No. : AO989216015

2.  The urine culture is used to diagnose a urinary
tract infection (UTI) and to identify the
pathogenic causing the infection.
 Germs can infect wounds and cause pain,
swelling, warm, and redness around the wound
and can lead to the formation of pus .

3.  To test and analyze urine sample,
pus sample , stool culture and blood
culture to identify the microorganisms
causing infection (if present) and
performing antibiotic sensitivity test
(AST).

4.  COLLECTION OF THE SAMPLE
FOR URINE / STOOL CULTURE:
20-30ml of the sample is collected in a sterile plastic
container.
FOR PUS SAMPLE :
A sterile cotton swab is used to collect pus from a
wound site.

5.  Plating and streaking
After the collection of the sample it is streaked on first
the macConky agar plate then onto the nutrient plate.
 Incubation

7.  Colony of a plate is mixed thoroughly with
sterile saline solution .
 Streaked with cotton swab on a MHA PLATE
or nutrient agar plate .
 Antibiotic discs are placed gently with the
help of forceps.

9.  ANTIBIOTIC SENITIVITY is done only when the
results are positive that is there is presence of
infection .
 IDENTIFICATION is a very important step
Like shiny colonies , rough colonies and colorful
colonies etc.
 Antimicrobial susceptibility tests (ASTs) basically
measures the ability of an antibiotic or other
antimicrobial agent to inhibit the invitro microbial
growth.