APOPTOSIS - Katha Sanyal
•
0 likes•100 views
Apoptosis is PCD- Programmed Cell Death wherein cells sends out signals to kill itself i.e., Cell Suicide. It is triggered by both Extrinsic and Intrinsic factors. The presentation covers it all.
Recommended
Recommended
More Related Content
Similar to APOPTOSIS - Katha Sanyal
Similar to APOPTOSIS - Katha Sanyal (20)
Recently uploaded
Recently uploaded (20)
APOPTOSIS - Katha Sanyal
- 1. APOPTOSIS G U I D E D B Y M S S U L A G N A G H O S H P R E S E N T E D B Y K A T H A S A N Y A L M S C B I O T E C H N O L O G Y S E M I S E S S I O N 2 0 2 1 PA P E R I
- 2. SYNOPSIS •Programmed Cell Death •Living Cell vs Necrotic Cell vs Apoptotic Cell •Biochemical Features •Mechanism: -Intrinsic Pathway -Extrinsic Pathway •Application of Apoptosis
- 5. PROGRAMMED CELL DEATH Vs ACCIDENTAL CELL DEATH
- 6. Fig 1: Necrosis vs Apoptosis
- 7. Fig 2: Normal vs Apoptotic Cell. Cooper & Hausman (2007); The Cell A Molecular Approach, 4th Edition, 691 Fig 3: Phosphotidylserine arrangement. Cooper & Hausman (2007); The Cell A Molecular Approach, 4th Edition, 692
- 8. LIVE vs NECROTIC vs APOPTOTIC CELL
- 9. LIVING CELL vs NECROTIC CELL vs APOPTOTIC CELL Phospholipid Composition Inactive Caspase Cell Intact Chromatin DNA linear Cell is at equilibrium Phospholipid Composition Same as Living Cell Independent of Caspase Cell Swells and Bursts Post-lytic DNA fragmentation Uncontrolled Degradative process Phospholipid composition Active Caspase Cell Shrinks Chromatin Condensed Pre-lytic DNA fragmentation Changes in the mitochondrial membrane potential Decrease in cytosolic pH Controlled energy dependent implosion Fig 3.1 + 3.2: Phosphotidylserine arrangement. Cooper & Hausman (2007); The Cell A Molecular Approach, 4th Edition, 692
- 10. Fig 4: Apoptosis Fig 5: Necrosis
- 12. PROTEIN CLEAVAGE PREOTEIN CROSS LINKING CELL SURFACE MARKERS CASPASES -A family of cysteine proteases -Present in an inactive proenzyme form Transglutaminase Trans-GLU-taminase Annexin I Calreticulin Homeostasis INITIATOR Cas-2 Cas-8 Cas-9 Cas-10 EXECUTIONER Cas-3 Cas-6 Cas-7
- 14. In the absence of Growth Signal Fig 7: Source- Cooper & Hausman (2007); The Cell A Molecular Approach, 4th Edition, 695 Fig 6: Bcl-2 Pathway
- 15. cytochrom C released In the absence of Growth Signal Fig 7: Source- Cooper & Hausman (2007); The Cell A Molecular Approach, 4th Edition, 695 Fig 6: Bcl-2 Pathway
- 16. Bax creates channels In the Mitochondrial Membrane Cytochrome C oozes Out of Mitochondria Cytochrome C binds with Apaf-1 and Procaspase-9 APOPTOSOME Cas-3 Executioner caspase activation by proteolytic cleavage APOPTOSIS Fig 8: Intrinsic Pathway (Complete)
- 17. LET’S RECALL DNA DAMAGE SIGNALLING AND PI3 PATHWAY
- 18. Fig 9: Source- Cooper & Hausman (2007); The Cell A Molecular Approach, 4th Edition, 698 Fig 10: Source- Cooper & Hausman (2007); The Cell A Molecular Approach, 4th Edition, 699
- 20. Plasma Membrane of CD8 Cell FAS Ligand TNFR Fig 11: Extrinsic Pathway
- 21. Conformational changes Fig 12: Conformational Changes in Extrinsic Pathway TNF
- 22. APOPTOSIS Cas-8 initiating Cas-3 executioner caspases via proteolytic cleavage FADD + Procaspase 8 Fig 13: TNF Pathway Fig 14: Perforin-Granzyme Pathway Directly cleaves DNA or activates Cas-10
- 23. APPLICATION
- 24. OVERPRODUCTION OF CELLS HOMEOST- -ASIS PATHOGEN INVADED CELSS ACTIVATED OR AUTO- AGGRESSIVE IMMUNE CELLS REMODE- -LLING CENTRE ORGAN SCULPTING CTRL
- 25. REFERENCES
- 26. Cooper G. M. and Hausman R. E.(2007), The cell: a molecular approach, 4th Edition, 695-701 Karp G.(2013), CELL AND MOLECULAR BIOLOGY, 7th Edition, 656-659 Bruce Alberts [and seven others] (2014), ESSENTIAL CELL BIOLOGY, 4th Edition, 633-636 Elmore S.(2007), APOPTOSIS: A REVIEW OF PROGRAMMED CELL DEATH, Published in final edited form as: Toxicol Pathol, 495–516
- 27. THANK YOU