BOC 134 lecture - 7 Cell Death

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Lecture 7, BIOL 134

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  • Dear New Friends of my Father Jesus;

    I know that most of you are not aware that after the 7 Seals are opened on the Book of Life of the Lamb that he ends up sitting down on the Throne of God. But it is this signal truth that explains what the arrival of the New Heaven and New Earth is really caused by: a New Lord God of a New Creation: not the mundane and fallen status quo of mortality that is our 'last captivity'. The final level of the providence is then that as Earth as 'Egypt'; Heaven as 'the Promised Land'; and Death as the 'Cosmic Pharaoh' whose 'captivity' for the Third Israel is of 'Mortality' with the 'Wall' between North and South Korea symbolizing the 'barrier of Original Sin' to our reaching 'Immortality in the Kingdom' on the other side; as we go from 'Earth/Egypt' to 'Heaven/as the first course of Restoration into Canaan at the Cosmic Level'.

    Hyo Jin Moon, in whom I am in contact with from spirit world, said I should include this quote from Luke 12:45-48; as explaining my role in relation to that of Rev. Moon. The main point is the Final Test for Rev. Moon as of when the servant John of my Father the Lord God who is sent in his Lord's place with a scribe and finds Rev. Moon as ' the faithful and wise steward' say in his heart these words: to wit;

    '...My lord delayeth his coming..';
    ( by the endless prolongation of the Will)

    and shall begin to beat the menservants and maidens,

    and to eat and drink, and to be drunken;
    The lord of that servant will come in a day when he looketh not for him;

    and at an hour when he is not aware,

    and will appoint him his portion with the unbelievers.
    And that servant (( Rev. Moon )) which knew his lord's will,

    and prepared not himself

    ( by not keeping or regaining his virginity ),

    neither did according to His Will

    ( by gathering the 144,000 virgins of God and the Lamb

    who were the eunuchs who would not marry nor be given in marriage),

    shall be beaten with many stripes.
    Here Hyo Jin Moon says this applies to me and My Generation:
    'But he who knew not, and DID commit things worthy of stripes,

    shall be beaten with few stripes.
    And this is where my Father's servant John chimed in with Hyo Jin:

    'For unto whomsoever MUCH IS GIVEN

    ( which obviously refers to Rev. Moon),

    of him shall much be required

    ( as being given the greater portion of responsibility ):

    and to whom men have committed much

    ( because the members of the Unification

    Church sacrificed so much to pay so much indemnity),
    of him (( Rev. Moon )) they will

    ASK THE MORE

    (( by having Rev. Moon be restored by John and me in conjunction with Hyo Jin Moon having brought us all together in 'Unity'; so his father can become the Messiah )).'

    I know that you and the members of the Unification Church do not want to hear anything that challenges their beliefs or views about a man they say is 'God' and the 'Messiah'; even as he prepares to die and be interred in a huge tomb; from which there shall be no resurrection.

    In my book 'A City Not Forsaken: My Generation and the Revelation of Salvation' I point out Three Basic Great Errors Rev. Moon made in the book 'Divine Principle'; and also where he 'stumbled' and then at the end when he 'fainted'.

    Please read this and then; with your grasp of the Principle; answer me: as if you indeed stood in the Presence of the Lamb and the 12 Apostles; the Judge and the Jury, if you will.

    In truth you already are standing in judgment: because I simply am not only what I appear to be; and I do know whereof I speak. I am the Wrath of the Lamb as the Child of the Resurrection; having the Measure '144' as symbolizing 'the Wall' as of that Virginity itself by which I was redeemed from the earth.

    Here are some excerpts for my new friends to peruse:.


    Thus the First Stumbling Rev. Moon made in 1960 is that he did not Marry a Black American Woman who symbolizes the Soul of America in the Position of Sheba as the queen of the South; thus leaving her as the Widow of Israel, Rev. Moon could have done what Elijah once appeared to do for the previous Widow of Israel in Zarepta.

    To substantially symbolize what mixing the soil of Korea with that of our Nation really could really been, having laid 'the Foundation of Salvation' here in America could of happened if only Re. Moon had 'marched' or 'walked' with our 'spiritual king' of non-violence; Dr Martin Luther King; but Moon was too busy with J.Edgar Hoover branding Dr. King as 'pro-Communist', another accusation of the Devil Rev. Moon propagated due to his jealousy of the Third Israel as the Black Christian Race in the West and especially it's black Moses.

    Because Rev. Moon never 'arrived' as our 'Joshua' is the reason why so many of the children of all the tribes of Israel died; both Rev. Moon's own family members and those of My Generation.

    The First Great Mistake in the book 'Divine Principle' is that Rev. Moon said that my Father Jesus did not arise in the flesh; and bone; as of the Resurrection; as even Doubting Thomas had to admit; thus Rev. Moon lost his 'wedding garment' as having 'sold' it to buy the double-edged sword his book ''Divine Principle' really became; but it could have been a Scepter of Resurrection had he said he was Elijah!

    But Rev. Moon did not even rise to the standard of the least of the Apostle's that Thomas really was: no faith; no progress on the Path of the Just.

    The Second Great Mistake Rev. Moon made in turning his book 'Divine Principle' into a discarnate object of 'good and evil' was in saying that the Holy Spirit is a 'female spirit'; when the Seal ( had he reached it ) would have shown him that the Holy Spirit of God is Jehovah Himself; and this cannot be known until one has the Seal as I was given; to Seal the 144,000 that Rev. Moon Married when he was to Seal them instead; which would have been the Baptism of Fire where the Hate seen by the Arabs and the Jews in 1967 at the 144 hours of War and Hatred became seen opposed the the Summer of Love and Peace; yea! Love as Haight Street became our Jordan: he missed this at the Visitation of God, to begin to raise the 144,000 Virgins from My Generation: the Children of the Resurrection who do not marry nor are they to be given in marriage; which Rev. Moon ignored. Yet these virgins are the Clouds themselves who first rise: dead in Christ, faithful into Death.

    It is We who are the Clouds; the Virgins unto God and the Lamb; and I speak for them as the least: for we stand for the Bride my City really is: a City Not Forsaken it shall be called.

    The Third Great Mistake Rev. Moon made was in then 'Marrying' the Lamb to the Holy Spirit as his 'Bride'; being as how the Holy Spirit is that of Jehovah Himself, as all of they called to the Table of Justice know.

    Here Rev. Moon allowed Love and Truth as the 2 Witnesses to be invaded by Satan as they symbolized the Bride and the Bridegroom; the Bride being she who was to abide in Love and thus God to 'prepare herself' for the Bridegroom; what happened instead is that SODOM arose by Rev. Moon marrying 2 'male entities' of the Son and the Shepherd; which is who the Holy Spirit really is.

    This was a misidentification that allowed 'Isis' who is 'Mystery' as the 'queen' of Egypt to invade his life and his book: a complete disaster. The men of Sodom are now trying to take America: state by state. They already invaded the Catholic Church due to Moon's blunder, where men as 'false father's' rape their own spiritual 'sons' as in the 'marrying' of 'Jesus the Son' to his 'Father the Holy Spirit who is Jehovah'' which condition allowed, and actually produced the Abomination of Desolation itself.

    And where Rev. Moon Fainted completely is where he decided that he would consciously speak the Accusation that John the Baptist dared not even admit to himself: but because he secretly feared it was true Satan invaded him and he died: the Accusation that his father Zacharias had committed an act of Adultery with Mary by which Jesus was conceived: and this is what 'killed' the Unification Church: the deadly Error of John that was the 'beam' in his 'eye'; the 'delusion' of his 'mind'; and THIS ALONE is the 'deadly wound' of our own 'John the Baptist'; Reverend Sun Myung Moon.

    In closing I can only hope you will want to respond to what I have written here point by point with a 'principled' point of view and not childish recriminations or attempts to simply brush me off or humor me until I die or vanish in a purff of smoke; or something just as melodramatic..

    Unfortunately no one in the Unification Church believes that what is written in the Revelation of Jesus Christ is now to be revealed as what it truly is: The Book of Life of the Lamb; but I would have Rev. Moon himself reveal it so that he can truly earn his place in the unfolding of the Design of Providence as the Messiah sent by God with the Word of God.

    That is what Rev. Moon was SUPPOSED to have done. But he declared the Revelation to be a 'fairytale; whose imagery and language were to awaken men of a much lower standard and understanding'

    Not so; please believe me.

    Peace; and Love; from the God of Love and Peace in whose Name my Father Jesus has had me appear to restore Rev. Moon before he dies.

    Salvation Rose
    The Bride; the Lamb's Wife
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BOC 134 lecture - 7 Cell Death

  1. 1. The Biology of Cancer Chapter 9: p53 and Apoptosis: Master Guardian and Executioner Cell Death
  2. 2. Apoptosis Autophagy Necrosis Programmed cell death. Death cycle is programmed by the cell itself ‘Self-eating’ Catabolic process involving lysosomes. ‘Death’ caused by external factors like trauma or toxins. Not programmed. Cell Death
  3. 3. The term was originally used by Wyllie and his colleagues and is from the Greek meaning “dropping away” as the leaves from a tree. Apoptosis: Programmed Cell Death Cell Suicide The Face of Cell Death: Apoptosis QuickTime™ and a decompressor are needed to see this picture. QuickTime™ and a decompressor are needed to see this picture.
  4. 4. QuickTime™ and a decompressor are needed to see this picture.
  5. 5. QuickTime™ and a decompressor are needed to see this picture.
  6. 6. QuickTime™ and a decompressor are needed to see this picture. QuickTime™ and a decompressor are needed to see this picture.
  7. 7. Active cell death • Specific gene expression • Specific intracellular signals • Requires energy and RNA and protein synthesis • Characteristic morphological features • DNA cleaved, chromatin condenses • Cells shrink • Formation of apoptotic body • Cleared by phagocytosis • No inflammation=no tissue damage Passive cell death • Physical or chemical trauma • Cells swell up • Membrane breaks down and cellular contents leak out • Nucleus disintegrates • Cell ghosts • Inflammatory=tissue damage Apoptosis Necrosis QuickTime™ and a decompressor are needed to see this picture. QuickTime™ and a decompressor are needed to see this picture.
  8. 8. Figure 9.18d The Biology of Cancer (© Garland Science 2007) An apoptotic cell showing fragmented Golgi bodies (green)
  9. 9. Autophagy
  10. 10. QuickTime™ and a decompressor are needed to see this picture.
  11. 11. QuickTime™ and a decompressor are needed to see this picture. Srcf.ucam.org Autophagy: clearing out garbage
  12. 12. QuickTime™ and a decompressor are needed to see this picture.
  13. 13. QuickTime™ and a decompressor are needed to see this picture. The Face of Cell Death: Apoptosis
  14. 14. QuickTime™ and a decompressor are needed to see this picture. Two Apoptotic Pathways: Extrinsic & Intrinsic
  15. 15. Two Pathways that Initiate Apoptosis Intrinsic/ Mitochondrial Apoptosis Regulated by Mitochondrial Cytochrome C release Extrinsic/ Death Receptor Apoptosis Activated by ligation of Death Receptors Fas, TNF alpha These pathways intersect at the effector caspases
  16. 16. QuickTime™ and a decompressor are needed to see this picture. Two Pathways that Initiate Apoptosis
  17. 17. QuickTime™ and a decompressor are needed to see this picture. Intrinsic Pathway Chemotherapy Irradiation
  18. 18. Figure 9.29 The Biology of Cancer (© Garland Science 2007) The Apoptotic Caspase Cascade: Apoptosome The Wheel of Death Pro-apoptotic signals open channels in the mitochondrial membrane to allow cytchrome c and Smac/Diablo molecules to be released. Cytochrome c molecules bind to Apaf1 to form the apoptosome. The apoptosome activates pro-caspase 9 to caspase 9 which in turn activate caspase 3. Caspase 3 activates in turn, a series of executioner caspases which cleave various death substrates whose cleavage creates the apoptotic cell phenotype.
  19. 19. Figure 9.28 The Biology of Cancer (© Garland Science 2007) The Wheel of Death - Apoptosome The apoptosome is assembled in the cytosol when cytochrome c molecules are released from the mitochondria into the cytosol.
  20. 20. QuickTime™ and a decompressor are needed to see this picture. The apoptosome associates with Apaf1. this causes the assembly of the 7 spoked wheel in which Apaf-1 forms the spokes and the cyt c forms the tips. Once assembled this attracts procaspase 9 into the hub of the wheel which converts procaspase 9 into active caspase 9. The resulting caspase 9 proceeds in turn to cleave and activate other caspase molecules thereby triggering the apoptotic cascade. Caspase 9 is activated
  21. 21. Caspase 9 activates the executioner caspases QuickTime™ and a decompressor are needed to see this picture. Active caspase 9
  22. 22. QuickTime™ and a decompressor are needed to see this picture. Executioner caspases : Caspase 3, 6 & 7 cleave DNA by activating DNAase QuickTime™ and a decompressor are needed to see this picture. APOPTOSIS
  23. 23. QuickTime™ and a decompressor are needed to see this picture. Executioner caspases : Caspase 3, 6 & 7 cleave DNA by activating DNAase QuickTime™ and a decompressor are needed to see this picture. APOPTOSIS Caspase 9 is activated Caspase 9 activates the executioner caspases Mitochondrial Cyt C release QuickTime™ and a decompressor are needed to see this picture. INTRINSIC APOPTOTIC PATHWAY
  24. 24. The Extrinsic Pathway: In the extrinsic pathway, signal molecules known as ligands, which are released by other cells, bind to transmembrane death receptors on the target cell to induce apoptosis. For example, the immune system’s natural killer cells possess the Fas ligand (FasL) on their surface. The binding of the FasL to Fas receptors (a death receptor) on the target cell will trigger multiple receptors to aggregate together on the surface of the target cell. The aggregation of these receptors recruits an adaptor protein known as Fas- associated death domain protein (FADD) on the cytoplasmic side of the receptors. FADD, in turn, recruits caspase-8, an initiator protein, to form the death-inducing signal complex (DISC). Through the recruitment of caspase-8 to DISC, caspase-8 will be activated and it is now able to directly activate caspase- 3, an effector protein, to initiate degradation of the cell. The extrinsic apoptotic pathway QuickTime™ and a decompressor are needed to see this picture.
  25. 25. QuickTime™ and a decompressor are needed to see this picture. The Extrinsic Apoptotic Pathway Disc (death inducing signal complex) TRADD : tumor necrosis factor death domain, FADD: Fas associated death domain. QuickTime™ and a decompressor are needed to see this picture. Cleaves DNA QuickTime™ and a decompressor are needed to see this picture.
  26. 26. Figure 9.19 The Biology of Cancer (© Garland Science 2007) Apoptosis of cells forming webs between future mouse toes. The dark dots are apoptotic cells.
  27. 27. QuickTime™ and a decompressor are needed to see this picture. Apoptotic humor !
  28. 28. p53 and Apoptosis
  29. 29. Figure 9.8 The Biology of Cancer (© Garland Science 2007) p53 - activating signals’s and p53 downstream effects A variety of cell physiologic stresses can cause a rapid increase in p53 levels which proceeds to induce a number of responses.
  30. 30. A cancer cell does not want to undergo apoptosis hence it initiates anti-apoptotic strategies
  31. 31. Figure 9.34 The Biology of Cancer (© Garland Science 2007) Anti-apoptotic strategies used by cancer cells Cancer cells resort to numerous strategies in order to decrease the likelihood of apoptosis. This diagram indicates that in various cancer cell types the levels or activity of important pro-apoptotic proteins are decreased (blue). Conversely, the levels or activity of certain anti-apoptotic proteins may be increased (red).
  32. 32. Figure 9.37 The Biology of Cancer (© Garland Science 2007) The Apoptotic Circuit Board The full array of components governing apoptosis is not known. An initial attempt at modeling the system is shown.
  33. 33. Cancer cells invent numerous ways to inactivate the apoptotic machinery in order to survive Among these are the activation of Akt pathway, inactivation of p53 and also inhibition of caspases Loss of apoptotic functions allows cancer cells to survive a variety of cell physiological stresses such as DNA damage

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