research associated with cancer and glucose metablism

1. Yuanchao Ye, Ph.D.
Postdoctoral fellow, Department of Pharmacology, Carver College of Medicine
University of Iowa
2254 E Grantview Dr. Coralville, IA, 52241
Tel. 319-383-6824
E-mail:yuanchaoye1986@gmail.com
Education:
Ph.D. in Pharmacology, Shenyang Pharmaceutical University, Shenyang, China,
09/2008-07/2012
B.S. in Pharmacy, Shenyang Pharmaceutical University, Shenyang, China, 09/2004-
07/2008
Post-doctoral training:
08/2012-present Department of Pharmacology, Carver College of Medicine,
University of Iowa, Iowa city, IA
Skills and Ability
1. Ability to manage time and work independently
2. Ability to collaborate with team member
3. Ability to prioritize and organize workload to efficiently accomplish goals
4. Ability multitask and maintain several projects at one time
5. Ability to perform a variety of duties without loss of efficiency
6. Strong attention to detail
7. Ability to perform repetitive work
8. Effective oral and written communication skills
9. Energetic and instinctive leadership capabilities
10. Quick learning ability
11. Lab management
Technical Expertise
1. Animal experiments
a. Handling and dosing mice (i.p., i.g., s.c., i.v.)
b. Basically mouse experiments and surgery
c. Xenograft
d. Transgenic mice and KO mice
e. Glucose tolerance test, Insulin tolerance test, Glucagon challenge test
f. Blood collection
g. Mice castrated
h. Experience in handling rabbit and rat
2. Cell biology
a. Cell culture (cell lines and primary cells)

2. b. Primary cells isolation (Epithelial cells from mammary gland; Fibroblast from
mice embryo; Hepatocytes from liver; Lymphocytes from spleen, whole
blood, bone marrow)
c. Development of stable transfected cell line
d. Cell transfection,
e. Flow cytometry,
f. Cell proliferation assay
g. Cell migration and invasion
h. Spheres and 3D culture
i. Virus construction and purification
j. Microscopy: light microscopy, fluorescence microscopy, laser scanning
confocal microscopy,
3. Molecular biology
a. Extraction of DNA and RNA, reverse transcription, PCR, qPCR, agarose gel
electrophoresis, gene cloning (Crisper system and Gateway system)
b. Western blot, immunohistochemistry, immunofluorescence, Elisa,
immunoprecipitation
4. Others
Database analysis (TCGA, c-bioportal); R studio, drug screening; protein
purification; Microsoft office (Excel, PowerPoint, Word, Access); Prism,
Chromatography, autophagy and apoptosis detection.
Awards and Honors:
2005 The Pharmaceutical Education Scholarship of the United Laboratories
2005-2008 The Scholarship of Shenyang Pharmaceutical University
2006 and 2007 Best Student in Shenyang Pharmaceutical University
2009 and 2011 Excellent Student in Shenyang Pharmaceutical University
2009 and 2011 The Enterprise Scholarship of Kangyuan
2011 Excellent Abstract in 1st Symposium on Development and Research of
Original New Drug
Academic experience and Professional Memberships:
2014- Present Reviewer, Archives of Medical Science
2014- Present Reviewer, Tumor Biology
2014- Present Reviewer, BMC Beterinary Research
2015- Present Reviewer, Acta Pharmacologica Sinica
2015- Present Reviewer, Journal of Microbiology biotechnology and Food sciences
2016- Present Reviewer, Jourmal of Chinese Pharmaceutical Sciences
2014- Present Member, American Association for Cancer Research

3. SelectedPeer-reviewed Publications
1. Yuanchao Ye, Xiaoyun Tang, Zhizeng Sun, Songhai Chen. Upregulated WDR26
serves as a scaffold to coordinate PI3K/ AKT pathway-driven breast cancer cell
growth, migration, and invasion. Oncotarget. 2016;7(14):17854-17869. DOI:
10.18632/oncotarget.7439
2. Yuanchao Ye, Zhizeng Sun, Ang Guo, Lang-sheng Song, Justin L Grobe, Songhai
Chen. Ablation of GNB3 gene does not affect blood pressure, body weight and
metabolism, but causes bradycardia. Cellular Signalling. 2014; 26(11): 2514-2520.
DOI: 10.1016/j.cellsig.2014.07.030
3. Yuanchao Ye, Hongju Wang, Weiwei Liu, Ling Chen, Shin-ichi Tashiro, Satoshi
Onodera, Takashi Ikejima. Negatively-regulated necroptosis by autophagy required
caspase-6 activation in TNFα-treated murine fibrosarcoma L929 cells. Int
Immunopharmacol. 2013; 17(3):548-555.
4. Yuanchao Ye, Hongju Wang, Lu Yu, Shin-ichi Tashiro, Satoshi Onodera, Takashi
Ikejima. RIP1-mediated mitochondrial dysfunction and ROS production contributed
to tumor necrosis factor alpha-induced L929 cell necroptosis and autophagy. Int
Immunopharmacol.2012;14(4):674-682.
5. Yuanchao Ye, Hongju Wang, Lei Xu, Weiwei Liu, Binbin Liu, Shin-ichi Tashiro,
Satoshi Onodera, Takashi Ikejima. Oridonin induced apoptosis and autophagy partly
through nitric oxide-ERK-p53 positive feedback loop signaling pathway in murine
fibrosarcoma L929 cells. Acta Pharmacol Sin. 2012;33(8):1055-1061.
6. Yuanchao Ye, Lu Yu, Hongju Wang, Shin-ichi Tashiro, Satoshi Onodera, Takashi
Ikejima. TNFα-induced necroptosis and autophagy via supression of p38-NF-κB
survival pathway in L929 cells. J Pharmacol Sci. 2011;117(3):160-169.
7. Weiwei Liu, Wuxiyar Otkur, Yuning Zhang, Qiuyuan Li, Yuanchao Ye, Linghe
Zang, Hao He, Toshihiko Hayashi, Shin-ichi Tashiro, Satoshi Onodera, Takashi
Ikejima. Silibinin protects murine fibroblast L929 cells from UVB-induced apoptosis
through the simultaneous inhibition of ATM-p53 pathway and autophagy. FEBS J.
2013; 280(18):4572-4584.
8. Weiwei Liu W, Wuxiyar Otkur, Lingzhi Li, Qiong Wang, Hao He, Yuanchao Ye,
Yuning Zhang, Toshihiko Hayashi, Shin-ichi Tashiro, Satoshi Onodera, Takashi
Ikejima. Autophagy induced by silibinin protects human epidermoid carcinoma
A431 cells from UVB-induced apoptosis. J Photochem Photobiol B. 2013;123:23-
31.
9. Qiong Wang, Weiwei Liu, Hong Zeng, Xuqin Xie, Guangxi Zang, Yuanchao Ye,
Shin-ichi Tashiro, Satoshi Onodera, Takashi Ikejima. p53-mediated autophagy
adjustment is involved in the protection of silibinin against murine dermal
inflammation and epidermal apoptosis induced by UVB irradiation. J Asian Nat Prod
Res. 2013;15(2):117-129.
10. Ynag Yu, Simiao Fan, Yuanchao Ye, Shin-ichi Tashiro, Satoshi Onodera, Takashi
Ikejima. The tyrphostin AG1478 augments oridonin-induced A431 cell apoptosis by

4. blockage of JNK MAPK and enhancement of oxidative stress. Free Radic Res.
2012;46(11):1393-1405.
11. Linghe Zang, Hao He, Yuanhcao Ye, Weiwei Liu, Simiao Fan, Shin-ichi Tashiro,
Satoshi Onodera, Takashi Ikejima. Nitric oxide augments oridonin-induced
phagocytosis of apoptotic cells by human histocytic lymphoma U937 cells via
autophagy and the NF-κB-COX-2-IL-1β pathway. Free Radic Res.
2012;46(10):1207-1219.
12. QiongWang, Yunachao Ye, Weiwei Liu, Shan Jiang, Shin-ichi Tashiro, Satoshi
Onodera, Fengying Gu, Yonggang Wang, Takashi Ikejima. Dual effects of silibinin
treatment on autophagy-regulated dermal apoptosis retardation and epidermal
apoptosis up-regulation in UVB-induced skin inflammation. J Asian Nat Prod Res.
2012;14(7):688-699.
13. Ying Liu, Ying Yang, Yuanchao Ye, Kuan Cai, Qifeng Shi, Shin-ichi Tashiro,
Satoshi Onodera, Takashi Ikejima. Activation of ERK-p53 and ERK-mediated
phosphorylation of Bcl-2 are involved in c-Met inhibitor SU11274-induced
autophagic cell death in human lung cancer A549 cells. J Pharmacol Sci.
2012;118(4):423-432.
14. Ying Liu, Qifeng Shi, Yuanchao Ye, Shin-ichi Tashiro, Satoshi Onodera, Takashi
Ikejima. Activated O2˙- and H2O2 mediated cell survival in SU11274-treated non-
small-cell lung cancer A549 cells via c-Met-PI3K and c-Met-Grb2/SOS-Ras-p38
pathways. J Pharmacol Sci. 2012;119(2):150-159.
15. Linghe Zang, Qian Xu, Yuanchao Ye, Yanqiu Liu, Shin-ichi Tashiro, Satoshi
Onodera, Takashi Ikejima. Autophagy enhanced phagocytosis of apoptotic cells by
oridonin-treated human histocytic lymphoma U937 cells. Arch Biochem Biophys.
2012;518(1):31-41.
16. Binbin Liu, Pengfei Yang, Yuanchao Ye, Yan Zhou, Lingzhi Li, Shin-ichi Tashiro,
Satoshi Onodera, Takashi Ikejima. Role of ROS in the protective effect of silibinin
on sodium nitroprusside induced apoptosis in rat pheochromocytoma PC12 cells.
Free Radic Res. 2011;45(7):835-47.
17. Yan Cheng, Feng Qiu, Yuanchao Ye, Shin-ichi Tashiro, Satoshi Onodera, Takashi
Ikejima. Oridonin induces G2/M arrest and apoptosis via activating ERK–p53
apoptotic pathway and inhibiting PTK–Ras–Raf–JNK survival pathway in murine
fibrosarcoma L929 cells. Arch Biochem Biophys. 2009;490(1):70-75.
18. Yan Cheng, Feng Qiu, Yuanchao Ye, Zhaoming Guo, Shin-ichi Tashiro, Satoshi
Onodera, Takashi Ikejima.. Autophagy inhibits reactive oxygen species-mediated
apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-
treated murine fibrosarcoma L929 cells. FEBS J. 2009;276(5):1291-1306.
Conference and reports
1. Yuanchao Ye, Songhai Chen. Upregulation of WDR26 promotes breast cancer cell
growth, migration and invasion via enhancing PI3K/AKT signaling. American
Association for Cancer Research. 2014.

5. 2. Yuanchao Ye, Takashi Ikejima. Oral report: TNFα-induced necroptosis and
autophagy via supression of p38-NF-κB survival pathway in L929 cells. 1st
Symposium on Development and Research of Original New Drug. 2011.07
3. Yuanchao Ye, Lu Yu, Yan Cheng, Hongju Wang, Shin-ichi Yashiro, Satoshi
Onodera, Takashi Ikejima. Oral report: TNFα induced L929 cell necroptosis by
inhibitig p38-mediated NF-κB survival pathway and promoting the release of
cytochrome c. The International Conference of Pharmacology. 2010.09
References:
Takashi Ikejima, Ph.D.
Professor of Pharmacology
Phone: +86-24-23844463
E-mail: ikejimat@vip.sina.com
Caitlin Runne, Ph.D.
Subject Matter Expert, eScience Lab
Phone: 720-708-8141
E-mail:Caitlin.Runne1@gmail.com
Jianqi Yang, Ph.D.
Scientist
Phone: 319-512-9215
E-mail:jianqi-yang@uiwoa.edu