Pattarawut Sopha has extensive education and research experience in biological sciences. He received his Ph.D. in Biological Sciences from Nara Institute of Science and Technology in Japan, and holds an M.Sc. in Molecular Genetics and Genetic Engineering from Mahidol University in Thailand and a B.Sc. in Microbiology from Srinakarinwirot University in Thailand. His research has focused on protein homeostasis, the unfolded protein response, and ER protein quality control. He has worked as a postdoctoral researcher at the University of North Carolina studying ER stress responses.

1. Pattarawut Sopha 2008-2012 Ph.D., Biological Sciences
Nara institute of science and technology, Japan
2002-2005 M.Sc., Molecular Genetics and Genetic Engineering
Mahidol University,Thailand
1998-2002 B.Sc., Microbiology
Srinakarinwirot University,Thailand
CurriculumVitae
Education
Program of Applied Biological Sciences,
Chulabhorn Graduate Institute
54 Kamphaeng Phet 6,Talat Bang Khen, Lak Si,
Bangkok 10210,THAILAND
pattarawut@cgi.ac.th, (+66)25541900 ext. 2643
Date of Birth: January 22nd, 1980
Nationality:Thai
Research Experience
DepartmentofBiology,FacultyofScience,
SrinakarinwirotUniversity,Bangkok,Thailand
Undergraduatestudent,Advisor:Dr.PrawatAungpraphapornchai
ThestudyofADIfragmentsofLactobacillusspp.
usingdegeneratedprimers
2001 - 2002
IMBG,MahidolUniversity,
NakornPathom,Thailand
Researchassistant,Advisor:Dr.WitoonTirasophon
CharacterizationofchimericyeastIre1pinSaccharomycescerevisiae
IMBG,MahidolUniversity,
NakornPathom,Thailand
Graduatestudent,Advisor:Dr.WitoonTirasophon
InvestigationofcatalyticpropertiesofhumanIre1αpinyeastmodel
DepartmentofBiochemistry,FacultyofScienceMahidolUniversity,
Bangkok,Thailand
Researchassistant,Advisor:Dr.MathurosePonglikitmongkol
Transcriptionalactivationanalysisofhumanpapillomavirus(HPV)oncoproteins(E6andE7),
mitochondrialDNAGeneticanalysisofThailandHornbills,
thestudyoftumorsuppressioneffectsinCurcumacomosaextracts
Instituteofmolecularbiologyandgenetics(IMBG),MahidolUniversity,
NakornPathom,Thailand
Researchassistant,Advisor:Dr.WitoonTirasophon
Thestudyofunfoldedproteinresponse(UPR)ininsectcell
underdenguevirus-persistentinfection
Narainstituteofscienceandtechnology,
Nara,Japan
Ph.D.student,Supervisor:Dr.KenjiKohno
StudyonanovelmammalianERtransmembraneJ-protein,DNAJB14
DepartmentofCellBiologyandPhysiology,SchoolofMedicine,
UniversityofNorthCarolina,ChapelHill,NorthCarolina,USA
PostdoctoralResearch,Supervisor:Dr.DouglasM.Cyr
Studyontheregulationofthetransmembrane
cheperonesunderacuteERstress
2003
2002 -2005
2005 - 2006
2006 - 2008
2008 - 2012
2013 - 2016
Protein Homeostasis,
Cell Stress Response and Regulation,
Endoplasmic Reticulum (ER) Protein Homeostasis,
Chaperone Expression and Function during Cell Stress,
Transmembrane Protein Quality Control,
Cancer Cell Stress Response
ResearchInterests
Lecturer/Researcher

2. References
Publications
The ER-transmembrane Hsp40 DNAJB12 is sensitive to acute ER stress and degraded before ER stressinduced
apoptosis. Pattarawut Sopha, HongYu Ren, and Douglas M. Cyr (Manuscript in prep.)
Restoration of R117H CFTR Folding and Function in Human Airway CellsThrough Combination
Treatment withVX-809 andVX-770. Martina Gentzsch, HongYu Ren, Scott A. Houck, Nancy L. Quinney, Deborah
M. Cholon, Pattarawut Sopha, Chaudhry IG, Jhuma Das, NikolayV. Dokholyan, Scott H. Randell, and Douglas M.
Cyr. Am J Physiol Lung Cell Mol Physiol. 2016. DOI: 10.1152/ajplung.00186.2016
VX-809 corrects folding defects in cystic fibrosis transmembrane conductance regulatorprotein through
action on membrane-spanning domain 1. HongYu Ren, Diane E. Grove, Oxana De La Rosa, Scott A. Houck,
Pattarawut Sopha, FredrickVan Goor, Beth J. Hoffman, and Douglas M. Cyr. Mol Biol Cell. 2013. 24(19): 3016-24.
A novel mammalian ER-located J-protein, DNAJB14, can accelerate ERAD of misfolded membrane
proteins. Pattarawut Sopha, Hiroshi Kadokura,Yo-heiYamamoto, MasatoTakeuchi, Michiko Saito, AkioTsuru,
and Kenji Kohno. Cell Struct. Funct. 2012. 37: 177-187.
Domain compatibility in Ire1 kinase is critical for the unfolded protein response. Juthakorn Poothong,
Pattarawut Sopha, Randal J. Kaufman, andWitoonTirasophon. FEBS Lett. 2010. 584: 3203 - 3208.
Isolation of lactic acid bacteria from fermented foods which have potential for tetramethylpyrazine
production. Prawat Aungpraphapornchai, Nat-hatai Sangobpun. and Pattarawut Sopha. Srinakarinwirot Sci. J.
2007. 23: 94 – 108
Presentation
Poster presentation:
The ER-transmembrane Hsp40 DNAJB12 mediates cross-talk between ERQC and apoptotic signaling
pathways to protect cells from acute proteotoxic stress. Federation of American Societies for Experimental Biology
(FASEB) – Summer Research Conferences (SRC) : From Unfolded Proteins in the Endoplasmic Reticulum to
Disease. June 2015 Saxtons river,Vermont, USA
Study of a novel mammalian transmembrane J-protein, DNAJB14. Federation of American Societies for
Experimental Biology (FASEB) – Summer Research Conferences (SRC) : From Unfolded Proteins in the
Endoplasmic Reticulum to Disease. June 2011 Saxtons river,Vermont, USA
in vivo characterization of chimeric yeast IRE1 in Saccharomyces cerevisiae. 29th Congress on Science and
Technology ofThailand. October 2003 Khonkaen University, Khonkaen,Thailand
Oral presentation:
ER Stress-dependent Degradation of theTransmembrane Hsp40 Sensitizes cells to Apoptosis. Cell Biology
and Physiology (CBP) Fusion Seminar. May 2015 UNC-Chapel Hill, NC, USA
Study of a novel transmembrane J-protein, DNAJB14. NAIST Global COE International StudentWorkshop
(Joint seminar: NAIST, UC-Davis, CAS-IGDB). November 2010 Osaka, Japan
Study of a novel transmembrane J-protein, DNAJB14.The Joint Seminar (NAIST-MU-NU) on Biosciences
& Biotechnology, February 2010 Mahidol University, Bangkok,Thailand
#1
Douglas M. Cyr, Professor of Cell Biology
University of North Carolina – Chapel Hill
Department of Cell Biology and Physiology, School of Medicine
Room 4312B, MBRB
Chapel Hill, NC 27516 USA
(+1) 919-843-4805
dmcyr@med.unc.edu
Relationship: Postdoc Supervisor
#2
Patrick J. Brenwald, Professor of Cell Biology
University of North Carolina – Chapel Hill
Department of Cell Biology and Physiology, School of Medicine
Room 4312C, MBRB
Chapel Hill, NC 27516 USA
(+1) 919-843-4995
pjbrennw@med.unc.edu
#3
Kadokura Hiroshi, Professor of Biochemistry
Tohoku University
2-1-1 Katahira, Aoba-ku, Sendai 980-8577 JAPAN
(+81) 022-217-5605
kadokura@tagen.tohoku.ac.jp
Relationship: PhD thesis committee
#4
Kohno Kenji, Professor of Molecular and Cell Genetics
Nara Institute of Science andTechnology,
8916-5 Ikoma, Nara 630-0192 JAPAN
(+81) 0743-72-5642
kkouno@bs.naist.jp
Relationship: Advisor
#5
Mathurose Ponglikitmongkol, Professor of Biochemistry
Mahidol University
Department of Biochemistry, Faculty of Science
272 Room B314, Rama 6 Rd., Ratchadevi, Bangkok 10400THAILAND
(+66) 2-201-5455
mathurose.pon@mahidol.ac.th
Relationship: Advisor
General molecular biology techniques e.g. gene cloning,
PCR and related applications; nucleotide analysis using
southern and northern blotting;protein purification and analysis;
bacterial, yeast and mammalian cell genetic manipulations;
polyclonal antibody production; Primary Lung Cell Culture
2001 Department of Biology, Faculty of Science,
Srinakarinwirot University, Bangkok,Thailand
Teaching assistant, Participating in laboratory lectures,
and preparing laboratory materials
2008 NAIST international scholarship
2004Thailand Pfizer foundation scholarship program for human
life science research.
Pfizer foundation, Bangkok,Thailand.
2003 The Royal Bangkok Sport Club (RBSC) scholarship for
graduate students.
The Royal Bangkok Sport Club, Bangkok,Thailand.
TheThailand Research Fund (TRF) granted from
Prof. Dr. Sakol Panyim.
The personal financial support from Prof. Dr. Sakol Panyim.
IMBG, Mahidol University, Nakorn Pathom,Thailand.
Scholarships
TeachingExperience
TechnicalSkills