SlideShare a Scribd company logo
1 of 15
Download to read offline
Jazmin Gómez Olarte
Tercer semestre 2020-1
Facultad de medicina, Universidad Pontificia Bolivariana
INTRODUCTION
Phillygenin is a component extracted from Forsythiae Fructus.
Forsythia Suspensa Forsythiae Fructus
Phillygenin
* Detoxify
* Therapeutic effects
INTRODUCTION
TLR4/MYD88/NF-kB pathway
LPS: Lipopolysaccharide
TLR: Toll-like receptors
PHI could inhibit the proinflammatory
reaction induced by LPS, in addition it
could inhibit protein expression in the
TLR4 / MyD88 / NF-κB signal pathway.
OBJECTIVE
Investigate if Phillygenin
(PHI) has a therapeutic
effect on liver fibrosis
caused by inflammation.
MATERIALES Y MÉTODOS
Para qué
Para cultivo de las células
LX2 (línea de las células
estrelladas hepáticas del
humano).
CULTIVO CELULAR Fundamento
Proceso mediante el que
las células pueden
cultivarse en condiciones
controladas.
Fundamento Para qué
ELISA
Enzyme-Linked
ImmunoSorbent Assay
MATERIALES Y MÉTODOS
Detección de los
factores inflamatorios
IL-6, IL-1β y TNF-α.
*Prueba para determinar
[Antígeno] o
[Anticuerpo] con uno de
ellos en fase sólida y el
otro en solución.
* Conjugación enzima-Ac
que genera un producto
detectable.
MATERIALES Y MÉTODOS
Fundamento
* Retrotranscripción de una hebra
de ARN en ADN.
* Uso de transcriptasa inversa.
RT-qPCR
Reacción en cadena de la polimerasa con
transcriptasa inversa
Para qué
Determinación de la expresión de
colágeno I y α-SMA (secreción de
actina del músculo liso α) y para
verificación del mecanismo de PHI en
la ruta de señal TLR4 / MyD88 / NF-κB
MATERIALES Y MÉTODOS
Usado para la
detección de una
proteína específica en
algún tejido.
WESTERN BLOT
* Análisis de α-SMA y
COL1.
* Análisis de TLR4,
MyD88, TAK1, p65,
p-p65, IκBα, p-IκBα
and IKKβ.
Para qué Fundamento
RESULTS
Untreated cells were used as control.
RESULTS
RESULTS
RESULTS
LPS can cause the expression of IL-6,
IL-1β and TNF-α and the expression of
collagen I, indicating that LPS could
induce inflammation and fibrosis in
LX2 cells.
PHI could inhibit the activation of LX2
cells and the expression of fibrotic
cytokines by inhibiting the
proinflammatory reaction induced by
LPS in the TLR4 / MyD88 / NF-κB
signaling pathway.
DISCUSSION
Author Concept
Liu et al.; Paik et al.
LPS can not only stimulate LX2 cells to
secrete inflammatory cytokines, but
also activate LX2 cells to express
fibrotic cytokines of α-SMA and COL1.
Jaeyeon Jung
It has been reported that inhibition of
NF-κB signaling pathway played an
important role in reducing liver fibrosis
reaction.
Chen et al.; Liu et al. LPS induced LX2 cells inflammation
model was successfully established
with upregulation of IL-1β, IL-6 and
TNF-α levels.
CONCLUSIONS
Some substances of herbal origin such
as phillygenin can produce effects on
inflammatory processes by interfering in
a specific signaling pathway and in this
way cause a therapeutic response on
some tissue that could be compromised.
LPS are one of the responsible for the
production of proinflammatory
cytokines in this case by the activation of
the TLR4 / MyD88 / NF-κB pathway
where it eventually causes liver tissue
damage.
Seminario biologia molecular jazmin gomez

More Related Content

Similar to Seminario biologia molecular jazmin gomez

Eicosanoids.pptx for first year medical students
Eicosanoids.pptx for first year medical studentsEicosanoids.pptx for first year medical students
Eicosanoids.pptx for first year medical studentsdinesh kumar
 
INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...
INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...
INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...melissazapataduran
 
Nrf2: A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...
Nrf2:  A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...Nrf2:  A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...
Nrf2: A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...LifeVantage
 
Sfn2016 abstracts
Sfn2016 abstractsSfn2016 abstracts
Sfn2016 abstractsVan Cheung
 
Seminario biomol
Seminario biomolSeminario biomol
Seminario biomolSydgold15
 
Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.
Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.
Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.Dmitri Popov
 
Thiru Sivakumaran - SRTP 2011
Thiru Sivakumaran - SRTP 2011Thiru Sivakumaran - SRTP 2011
Thiru Sivakumaran - SRTP 2011tsivaku2
 
Immunomodulatory Potential of Probiotic Lactobacillus casei
Immunomodulatory Potential of Probiotic Lactobacillus caseiImmunomodulatory Potential of Probiotic Lactobacillus casei
Immunomodulatory Potential of Probiotic Lactobacillus caseiKarthikeyanThirugnan3
 
Second Messenger(F-28).pptx
Second Messenger(F-28).pptxSecond Messenger(F-28).pptx
Second Messenger(F-28).pptxZubair Manzoor
 

Similar to Seminario biologia molecular jazmin gomez (20)

Eicosanoids.pptx for first year medical students
Eicosanoids.pptx for first year medical studentsEicosanoids.pptx for first year medical students
Eicosanoids.pptx for first year medical students
 
INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...
INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...
INTERLEUKIN-6 PLAYS A PROTECTIVE ROLE IN DEVELOPMENT OF CISPLATIN-INDUCED ACU...
 
Acute renal failure.
Acute renal failure. Acute renal failure.
Acute renal failure.
 
Nrf2: A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...
Nrf2:  A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...Nrf2:  A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...
Nrf2: A Master Regulator of Detoxification and also Antioxidant, Anti-inflam...
 
Final Abstract
Final Abstract Final Abstract
Final Abstract
 
Zz c0610-1003 diabetes-np_final
Zz c0610-1003 diabetes-np_finalZz c0610-1003 diabetes-np_final
Zz c0610-1003 diabetes-np_final
 
cytokines
cytokinescytokines
cytokines
 
Sfn2016 abstracts
Sfn2016 abstractsSfn2016 abstracts
Sfn2016 abstracts
 
Seminario biomol
Seminario biomolSeminario biomol
Seminario biomol
 
Leucotrienes
LeucotrienesLeucotrienes
Leucotrienes
 
Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.
Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.
Radiation Protection: Phospholipase C, LAMP and Phopholipase C, LAMP inhibition.
 
Thiru Sivakumaran - SRTP 2011
Thiru Sivakumaran - SRTP 2011Thiru Sivakumaran - SRTP 2011
Thiru Sivakumaran - SRTP 2011
 
Sk
SkSk
Sk
 
Immunomodulatory Potential of Probiotic Lactobacillus casei
Immunomodulatory Potential of Probiotic Lactobacillus caseiImmunomodulatory Potential of Probiotic Lactobacillus casei
Immunomodulatory Potential of Probiotic Lactobacillus casei
 
GW8510 manuscript
GW8510 manuscriptGW8510 manuscript
GW8510 manuscript
 
Immunosuppression
ImmunosuppressionImmunosuppression
Immunosuppression
 
Second Messenger(F-28).pptx
Second Messenger(F-28).pptxSecond Messenger(F-28).pptx
Second Messenger(F-28).pptx
 
Prostaglandins.docx
Prostaglandins.docxProstaglandins.docx
Prostaglandins.docx
 
PROJECT PROPOSAL
PROJECT PROPOSALPROJECT PROPOSAL
PROJECT PROPOSAL
 
presentación de seminario
presentación de seminario presentación de seminario
presentación de seminario
 

Recently uploaded

FAIRSpectra - Enabling the FAIRification of Analytical Science
FAIRSpectra - Enabling the FAIRification of Analytical ScienceFAIRSpectra - Enabling the FAIRification of Analytical Science
FAIRSpectra - Enabling the FAIRification of Analytical ScienceAlex Henderson
 
Terpineol and it's characterization pptx
Terpineol and it's characterization pptxTerpineol and it's characterization pptx
Terpineol and it's characterization pptxMuhammadRazzaq31
 
Thyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate ProfessorThyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate Professormuralinath2
 
GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body Areesha Ahmad
 
Porella : features, morphology, anatomy, reproduction etc.
Porella : features, morphology, anatomy, reproduction etc.Porella : features, morphology, anatomy, reproduction etc.
Porella : features, morphology, anatomy, reproduction etc.Cherry
 
Call Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort ServiceCall Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort Serviceshivanisharma5244
 
Cyanide resistant respiration pathway.pptx
Cyanide resistant respiration pathway.pptxCyanide resistant respiration pathway.pptx
Cyanide resistant respiration pathway.pptxCherry
 
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRingsTransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRingsSérgio Sacani
 
FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry
FAIRSpectra - Enabling the FAIRification of Spectroscopy and SpectrometryFAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry
FAIRSpectra - Enabling the FAIRification of Spectroscopy and SpectrometryAlex Henderson
 
Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....
Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....
Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....muralinath2
 
Phenolics: types, biosynthesis and functions.
Phenolics: types, biosynthesis and functions.Phenolics: types, biosynthesis and functions.
Phenolics: types, biosynthesis and functions.Cherry
 
Reboulia: features, anatomy, morphology etc.
Reboulia: features, anatomy, morphology etc.Reboulia: features, anatomy, morphology etc.
Reboulia: features, anatomy, morphology etc.Cherry
 
The Mariana Trench remarkable geological features on Earth.pptx
The Mariana Trench remarkable geological features on Earth.pptxThe Mariana Trench remarkable geological features on Earth.pptx
The Mariana Trench remarkable geological features on Earth.pptxseri bangash
 
development of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virusdevelopment of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virusNazaninKarimi6
 
Genetics and epigenetics of ADHD and comorbid conditions
Genetics and epigenetics of ADHD and comorbid conditionsGenetics and epigenetics of ADHD and comorbid conditions
Genetics and epigenetics of ADHD and comorbid conditionsbassianu17
 
Concept of gene and Complementation test.pdf
Concept of gene and Complementation test.pdfConcept of gene and Complementation test.pdf
Concept of gene and Complementation test.pdfCherry
 
Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds
Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune WaterworldsBiogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds
Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune WaterworldsSérgio Sacani
 
Dr. E. Muralinath_ Blood indices_clinical aspects
Dr. E. Muralinath_ Blood indices_clinical  aspectsDr. E. Muralinath_ Blood indices_clinical  aspects
Dr. E. Muralinath_ Blood indices_clinical aspectsmuralinath2
 
PODOCARPUS...........................pptx
PODOCARPUS...........................pptxPODOCARPUS...........................pptx
PODOCARPUS...........................pptxCherry
 

Recently uploaded (20)

FAIRSpectra - Enabling the FAIRification of Analytical Science
FAIRSpectra - Enabling the FAIRification of Analytical ScienceFAIRSpectra - Enabling the FAIRification of Analytical Science
FAIRSpectra - Enabling the FAIRification of Analytical Science
 
Terpineol and it's characterization pptx
Terpineol and it's characterization pptxTerpineol and it's characterization pptx
Terpineol and it's characterization pptx
 
Thyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate ProfessorThyroid Physiology_Dr.E. Muralinath_ Associate Professor
Thyroid Physiology_Dr.E. Muralinath_ Associate Professor
 
GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body GBSN - Microbiology (Unit 3)Defense Mechanism of the body
GBSN - Microbiology (Unit 3)Defense Mechanism of the body
 
Porella : features, morphology, anatomy, reproduction etc.
Porella : features, morphology, anatomy, reproduction etc.Porella : features, morphology, anatomy, reproduction etc.
Porella : features, morphology, anatomy, reproduction etc.
 
Site Acceptance Test .
Site Acceptance Test                    .Site Acceptance Test                    .
Site Acceptance Test .
 
Call Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort ServiceCall Girls Ahmedabad +917728919243 call me Independent Escort Service
Call Girls Ahmedabad +917728919243 call me Independent Escort Service
 
Cyanide resistant respiration pathway.pptx
Cyanide resistant respiration pathway.pptxCyanide resistant respiration pathway.pptx
Cyanide resistant respiration pathway.pptx
 
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRingsTransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
TransientOffsetin14CAftertheCarringtonEventRecordedbyPolarTreeRings
 
FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry
FAIRSpectra - Enabling the FAIRification of Spectroscopy and SpectrometryFAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry
FAIRSpectra - Enabling the FAIRification of Spectroscopy and Spectrometry
 
Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....
Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....
Human & Veterinary Respiratory Physilogy_DR.E.Muralinath_Associate Professor....
 
Phenolics: types, biosynthesis and functions.
Phenolics: types, biosynthesis and functions.Phenolics: types, biosynthesis and functions.
Phenolics: types, biosynthesis and functions.
 
Reboulia: features, anatomy, morphology etc.
Reboulia: features, anatomy, morphology etc.Reboulia: features, anatomy, morphology etc.
Reboulia: features, anatomy, morphology etc.
 
The Mariana Trench remarkable geological features on Earth.pptx
The Mariana Trench remarkable geological features on Earth.pptxThe Mariana Trench remarkable geological features on Earth.pptx
The Mariana Trench remarkable geological features on Earth.pptx
 
development of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virusdevelopment of diagnostic enzyme assay to detect leuser virus
development of diagnostic enzyme assay to detect leuser virus
 
Genetics and epigenetics of ADHD and comorbid conditions
Genetics and epigenetics of ADHD and comorbid conditionsGenetics and epigenetics of ADHD and comorbid conditions
Genetics and epigenetics of ADHD and comorbid conditions
 
Concept of gene and Complementation test.pdf
Concept of gene and Complementation test.pdfConcept of gene and Complementation test.pdf
Concept of gene and Complementation test.pdf
 
Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds
Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune WaterworldsBiogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds
Biogenic Sulfur Gases as Biosignatures on Temperate Sub-Neptune Waterworlds
 
Dr. E. Muralinath_ Blood indices_clinical aspects
Dr. E. Muralinath_ Blood indices_clinical  aspectsDr. E. Muralinath_ Blood indices_clinical  aspects
Dr. E. Muralinath_ Blood indices_clinical aspects
 
PODOCARPUS...........................pptx
PODOCARPUS...........................pptxPODOCARPUS...........................pptx
PODOCARPUS...........................pptx
 

Seminario biologia molecular jazmin gomez

  • 1. Jazmin Gómez Olarte Tercer semestre 2020-1 Facultad de medicina, Universidad Pontificia Bolivariana
  • 2. INTRODUCTION Phillygenin is a component extracted from Forsythiae Fructus. Forsythia Suspensa Forsythiae Fructus Phillygenin * Detoxify * Therapeutic effects
  • 3. INTRODUCTION TLR4/MYD88/NF-kB pathway LPS: Lipopolysaccharide TLR: Toll-like receptors PHI could inhibit the proinflammatory reaction induced by LPS, in addition it could inhibit protein expression in the TLR4 / MyD88 / NF-κB signal pathway.
  • 4. OBJECTIVE Investigate if Phillygenin (PHI) has a therapeutic effect on liver fibrosis caused by inflammation.
  • 5. MATERIALES Y MÉTODOS Para qué Para cultivo de las células LX2 (línea de las células estrelladas hepáticas del humano). CULTIVO CELULAR Fundamento Proceso mediante el que las células pueden cultivarse en condiciones controladas.
  • 6. Fundamento Para qué ELISA Enzyme-Linked ImmunoSorbent Assay MATERIALES Y MÉTODOS Detección de los factores inflamatorios IL-6, IL-1β y TNF-α. *Prueba para determinar [Antígeno] o [Anticuerpo] con uno de ellos en fase sólida y el otro en solución. * Conjugación enzima-Ac que genera un producto detectable.
  • 7. MATERIALES Y MÉTODOS Fundamento * Retrotranscripción de una hebra de ARN en ADN. * Uso de transcriptasa inversa. RT-qPCR Reacción en cadena de la polimerasa con transcriptasa inversa Para qué Determinación de la expresión de colágeno I y α-SMA (secreción de actina del músculo liso α) y para verificación del mecanismo de PHI en la ruta de señal TLR4 / MyD88 / NF-κB
  • 8. MATERIALES Y MÉTODOS Usado para la detección de una proteína específica en algún tejido. WESTERN BLOT * Análisis de α-SMA y COL1. * Análisis de TLR4, MyD88, TAK1, p65, p-p65, IκBα, p-IκBα and IKKβ. Para qué Fundamento
  • 9. RESULTS Untreated cells were used as control.
  • 12. RESULTS LPS can cause the expression of IL-6, IL-1β and TNF-α and the expression of collagen I, indicating that LPS could induce inflammation and fibrosis in LX2 cells. PHI could inhibit the activation of LX2 cells and the expression of fibrotic cytokines by inhibiting the proinflammatory reaction induced by LPS in the TLR4 / MyD88 / NF-κB signaling pathway.
  • 13. DISCUSSION Author Concept Liu et al.; Paik et al. LPS can not only stimulate LX2 cells to secrete inflammatory cytokines, but also activate LX2 cells to express fibrotic cytokines of α-SMA and COL1. Jaeyeon Jung It has been reported that inhibition of NF-κB signaling pathway played an important role in reducing liver fibrosis reaction. Chen et al.; Liu et al. LPS induced LX2 cells inflammation model was successfully established with upregulation of IL-1β, IL-6 and TNF-α levels.
  • 14. CONCLUSIONS Some substances of herbal origin such as phillygenin can produce effects on inflammatory processes by interfering in a specific signaling pathway and in this way cause a therapeutic response on some tissue that could be compromised. LPS are one of the responsible for the production of proinflammatory cytokines in this case by the activation of the TLR4 / MyD88 / NF-κB pathway where it eventually causes liver tissue damage.