IATROSCAN MK6 TLC -FID/FPD

1. Recent Advances
In TLC
By Inder Ishwarlal Gohri 1

2. IATROSCAN MK6
TLC-FID/FPD Dual Detection
System
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3. > It is the combines techniques of thin layer chromatography
(TLC)
> It is an established technique for the separation of
organic compounds, with a flame ionization detector (FID)
>The new model IATROSCAN MK6 with FPD
(flame photometric detector) detects the hetero-atoms
such as phosphorous and sulfur selectively.
IATROSCAN
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4. >Theadditionof FPDto theformerTLC-FIDIATROSCANwithimprovedquantification
andreproducibilityenablestoacquirea newwealthof analyticalinformation
>It combinesthe advantagesof separationbyTLC(ofnon-volatiles)withdetectionby FID
(populardetectorin GC).
>It is mostideallysuitedfor SARAanalysisi.e., Saturates,Aromatics, Resinsand
Asphaltenesfoundin petroleumoil. It is an officialmethod(IP-469).
>MostpetroleumindustryresearchandrefiningunitsinIndiahaveinstalledthesame.
Biolipidsisanother areaof greatinterest.
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>A Flame ionization detector (FID) consists of a hydrogen
(H2)/air flame and a collector plate.
>The effluent from the column passes through the flame,
which breaks down organic molecules and produces ions.
>The ions are collected on a biased electrode and produce an
electrical signal.
>The FID is extremely sensitive with a large dynamic range,
its only disadvantage is that it destroys the sample.
>lame ionization detectors are used for detecting
hydrocarbons (HC) such as methane (CH4), ethane (C2H6),
acetylene (C2H2) etc.
Flame ionization detector
(FID)

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7. Flame photometric detector(FPD)
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1. The flame photometric detector (FPD) allows sensitive and selective measurements of volatile
sulphur and phosphorus compounds.
2. The detection principle is the formation of excited sulphur (S2*) and excited hydrogen
phosphorous oxide species (HPO*) in a reducing flame.
3. A photomultiplier tube measures the characteristic chemiluminescent emission from these
species.
4. The optical filter can be changed to allow the photomultiplier to view light of 394 nm for
sulphur measurement or 526 nm for phosphorus.
5. The detector response to phosphor is linear, whereas the response to sulphur depends on the
square of the concentration.

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ACCESSORIES AND
CONSUMABLES:
DEVELOPMEN
T TANK
CHROMAROD
DRYER
CHROMAROD

10. > They are the quartz rod coated with a thin layer of silica or alumina on which the
sample is developed and separated.
> After development, the drying is carried out with the help of a nitrogen flow or a
cool air blowing (air dryer)
>
The separated lipids are detected directly by combustion in a hydrogen flame, which
generates, as in GLC, an electric current
Chromarod
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1.Blank scan
> The CHROMAROD can be cleaned and activated by hydrogen flame through the
blank scan on IATROSCAN.

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2. Sample spotting
> Approx. 1 µl of the sample solution is spotted on the
CHROMAROD by means of a micro dispenser
Semi Automated Sample
Auto-spotter

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Full Automated Sample
Auto-spotter

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3. Separation
> The components in the sample on the CHROMAROD(s) are
separated through development procedure in the development
tank .
Development
Tank

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4.Solvent removal
> After development , the development solvent adsorbed on the
CHROMAROD(s) is removed.
CHROMARO
D DRYER

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5. Measurement
> After solvent removal start measurement by pressing START
key.

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Application

19. S.A.R.A. ANALYSIS
Saturates Aromatic Resins Asphaltenes
● Iatroscan MK6 TLC-FID is the fastest method on the market for S.A.R.A.
analysis!
● With Iatroscan MK6you cancharacterizePetroleumandBitumenin only30
seconds.
IatroscanMK6is quick, preciseandunexpensive!
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21. Sample:Standard mixture
1.Cholesterol ester
2.Triglyceride
3.Cholesterol
4.Phosphatidylethanolamine
5.Phosphatidylcholine
6.Phosphatidylinositol
7.Sphingomyelin
8.Lysophosphatidyl choline
Stationary phase :Chromarod-SIII
Mobile phase :
1st. Chloroform:Methanol:Water:
25%Ammonia
47:20:2.5:0.28 7cm
2nd.Hexane :Diethyl ether 63:7 10cm
Lipid Analysis
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22. Glycerides Analysis
Sample :Standard mixture
1.TG (tripalmitin)
2.FA (palmitic acid)
3.1,3-DG (1,3-dimyristin)
4.1,2-DG (1,2-dipalmitin)
5.MG (monopalmitin)
Stationary phase : CHROMAROD-
SIII
Mobile phase :
Benzene :Chloroform : Acetic acid
50 : 20 : 0.7 10cm
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23. >Special types of thin layers, such as those suitable for separating triglycerides
can be easily prepared.
>Possibility to work on 10 chromarod bars at the same time.
>Wide range of applications.
>Chromarod rods can be used up to 100 times.
>Short analysis time 20 -30 minutes depending on the method
> Neither coloring nor activation of thin layer is required for the analysis
Advantages
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REFERENCES:
1)https://www.anchrom.in/iatroscan.php
2)https://www.medience.co.jp/english/iatroscan.html
3)https://www.iatroscan.com/
4)https://www.sylant.pl/iatroscan-mk6-analizator-
tlcfid.html
5)http://shell-usa.com/Iatroscan/
6)http://hiq.linde-
gas.com/en/analytical_methods/gas_chromatogra
phy/flame_photometric_detector.html

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