NACGRAB Achievements 2017

1. NACGRAB’s 2017 Achievements on
Intermediate Outcome 2.1:
Strengthened Capacity of National Research Centres to Produce High Ratio, Cost Effective & Quality
Pre-basic Seed Yam For Seed Yam Producers Using A.S.
Under the Project
Yam Improvement for Incomes and Food Security in West Africa – Phase II
(YIIFSWA-II)
BY
Afolayan A. O. (PhD.)
@
THE ANNUAL PROGRESS REVIEW & WORK PLANNING MEETING OF YAM IMPROVEMENT FOR
INCOMES AND FOOD SECURITY IN WEST AFRICA-PHASE II (YIIFSWA-II)
BY BMGF 5TH - 8TH March 2018, IITA, IBADAN

2. INTRODUCTION
NACGRAB - established in 1987 by FMST
 Conduct research, gather data & disseminate technological
information on:
 Genetic resources conservation
 Genetic resources utilization
 Biotechnology application.
 Backed by Decree 33 of 1987 & Seed
Decree 72 of 1992.
 Expected to regulate seed, livestock &
fisheries industries (Varietal Release
Committees).
NACGRAB / IITA Collaborations
=> Mandate: Fostering
Relationship with National
GRCs & International
Organizations / Centres
(GRBA)

3. YIIFSWA-1 Collaboration
NACGRAB’s Achievements (2013 –2016)
1.) Establishment of
Collected Yam
Varieties in NACGRAB
Screen-House
2.) Research into
Appropriate Disinfection
Protocol for Yam In vitro
Regeneration
3.) Research into
Appropriate
media Protocols
for Yam In vitro
Regeneration
4.) Successful in
vitro regeneration of
Yam Explants (using
Meristem & Nodal
Cuttings)
5.) Virus Indexing
of Cultured Yam
plantlets
7.) Mass
Multiplication of
Virus Negative
Yam plantlets 8.) Bacterial
Indexing of Virus-
free Yam plantlets
6.) Sorting &
selection of Virus
Negative Yam plantlets
9a.) Mass
Multiplication of
clean Yam plantlets
(TIBs & CTC)
10.)Post-flask management
of clean Yam plantlets &
delivery of Disease-free Yam
seedlings to IITA
9b.) Plantlets
Phyto-sanitary
Re-Certification
Result
Acquisition
& Sorting
Result
Acquisition
& Sorting
Result Acquisition & Sorting

4.  Sub-Agreement: IITA /
NACGRAB (Signed in April, 2017):
*Intermediate Outcome 2.1:
Strengthened Capacity of
National Research Centres to
Produce High Ratio, Cost
Effective & Quality Pre-basic
Seed Yam to Meet the
Demands of Seed Yam
Producers Using Aeroponic
System.
NACGRAB / IITA Collaboration – YIIFSWA II

5.  NACGRAB (contribute to
achievement of Primary Outcome 2 of
YIIFSWA-II):
*Establishment of a functional,
scalable & sustainable seed
system driven by PSI
=> delivers sufficient quantities of
high quality seed of improved yam
varieties to farmers @ right time &
right prices that encourage adoption.
NACGRAB / IITA Collaboration – YIIFSWA II

6.  NACGRAB (contribute to
achievement of Primary Outcome 2 of
YIIFSWA-II):
* NACGRAB - work closely with
National Root Crops Research
Institute (NRCRI), Umudike
=>To supply the country need
in pre-basic seed yam by
producing high ratio, cost
effective & quality pre-basic
seed yam to meet the demand
of basic seed producers.
NACGRAB / IITA Collaboration – YIIFSWA II

7. 2017 NACGRAB Assigned Activities
 Output 2.1.2: One fully
functional TIBS established
 Output 2.1.6: Context/Sahel
Training on Business model
using TIBs
 Output 2.1.7: Capacity for
phyto-sanitary cleaning and
protection of in vitro plants (TC
and TIBs)

8. 2017 Proposed Work Plan
S/
N
ACTIVITY JA
N
FE
B
MA
R
APR MA
Y
JU
N
JU
L
AUG SPT OCT NO
V
D
E
C
1 Setting up a SETIS
Bioreactor facility
2 Rapid Multiplication in
CTC
3 Rapid Multiplication in
TIBs / SETIS
4 Post-flask activities
(Acclimatization and
weaning)
5 Delivery of Clean Yam
seedlings to NRCRI
Proposed plan on supply of Seedlings
 TDr 89 / 02665 (83, 6) – 1,200
 TDa 98/01176 (13) - 500
 TDr 95/19177 (21) - 240

9. SETIS Bioreactor System established
at NACGRAB
A
 24 pairs of SETIS™ Bioreactor
vessels,
 1 SETIS™ caps holder,
 100 SETIS™ air filter,
 3 SETIS™ T-air connectors &
components,
 1 SETIS™ Control/Software for 4
CPs,
 1 Set air filters (5/0,3/0,01μm)
 1 Air Dryer 220V - 400l/min (excl.
connections).
Official Handing-Over of SETIS by
Engr. Akeredolu (IITA) & his team
(May 6, 2017)

10. Alternative Power Generating Source
A
 Housing for inverter
 Inverter compatible AC (1.5Hp)
 LED lighting tubes (12pcs)
 Construction of housing for
compressor
 Installation of a separate
compressor for the SETIS
components
5KVA inverter system with eight
(8) 200A deep cycle batteries

11. Rapid Micro-propagation of Existing
Yam Plantlets from 2013-2016 YIIFSWA
A
B
Rapid multiplication of TDr 89/02665 using CTC technique
(Jan – April, 2017)

12. Acquisition of All Three Promoted Yam
Varieties from IITA (May 10, 2017)
A
B
TDr 89/02665 (83) – 6 plantlets
TDr 89/02665 (83) – 6 plantlets
TDr 89/02665 (83) – 6 plantlets
TDr 89/02665 (83) – 7 plantlets
TDr 89/02665 (6) – 3 plantlets
TDa 98/01176 (13) – 5 plantlets
TDr 95/19177 (21) – 7 plantlets
Total = 40 plantlets
In October, 2017: 1 bioreactor with TDr
95/19177 (21) + Some reagents etc.
 Seven (7) culture vials containing all
three promoted yam varieties with
phyto-sanitary batch numbers, were
given to NACGRAB/YIIFSWA team

13. Rapid Micro-propagation of YIIFSWA-
II Promoted Yam Varieties (CTC)
A
Rapid multiplication of forty (40) new yam plantlets supplied
from IITA, using CTC technique (May – Dec, 2017)

14. Trial Micro-propagation of YIIFSWA-
II Promoted Yam Varieties (SETIS)
A
Trial multiplication of yam plantlets using SETIS

15. Rapid Micro-propagation of YIIFSWA-II
Promoted Yam Varieties
S/N Variety Initial No. of
Virus-free
plantlets
Supplied by IITA
Total No. of
plantlets
Multiplied @
NACGRAB
Multiplicatio
n ratio /
variety
1. TDr 89/02665 (83) 25 1311 1:52
2. TDr 89/02665 (6) 3 489 1:163
3. TDa 98/01176 (13) 5 863 1:173
4. TDr 95/19177 (21) 127 704 1:6
Total seedlings 160 3367 1:21
Average % Contaminated Plantlets = 30.92 %

16. S/N Variety
Proposed plan
of supply of
Seedlings
Total No. of
plantlets
Multiplied @
NACGRAB
VBF Yam
Seedlings
supplied
1.
TDr 89/02665
(NBN.,83, 6)
1200 2673 1873
2.
TDa 98/01176
(13)
500 863 30
3.
TDr 95/19177
(21)
240 704 37
Total seedlings
1940
proposed
4240
Produced
1940
Supplied
Yam Varieties Supplied by NACGRAB in 2017
% Contaminated Plantlets = 30.92 %

17. NACGRAB / NRCRI Collaboration
A
B
NACGRAB gave NRCRI 862 disease-free TC and TIBs yam
seedlings

18. A
B
NACGRAB gave IITA 960 disease-free TC and TIBs yam seedlings
& 118 disease-free TC and TIBs yam plantlets
Supply of Disease-free Yam Variety to IITA

19. Training of NACGRAB Yam Team on
SETIS at IITA
A
B
12th - 16th of June, 2017 17th - 19th October, 2017

20. TDr
89/02665
(83)
CTC- 1: 4
TIBs- 1: 6
TDr
89/02665 (6)
CTC- 1: 4
TIBs- 1: 6
TDr
95/19177
CTC- 1: 5
TIBs- 1: 8
8- 10
weeks
TDa
98/01176
CTC- 1:6
TIBs- 1:10
TIBs Daily Immersion Frequency = 4
TIBs No. of Cycles Per day = 3 - 4 Cycles
% survival at post-flask Stage = 60 - 75%
% Contamination in TIBs = 50% % Contamination in CTC= 35%
Yam Propagation Ratio (In CTC & TIBs)

21. Yam Plantlets in Cultures (>Dec. 31, 2017)
0
20
40
60
80
100
120
140
160
180
200
TDr
89/02665
(83)
TDr
89/02665 (6)
TDa
98/01176
(13)
TDr
95/19177
(21)
Virus and Bacterial- Free Yam plantlets (Dec, 2017)
TDr 89/02665 (83)
TDr 89/02665 (6)
TDa 98/01176 (13)
TDr 95/19177 (21)
No.ofvirusandbacterialfreeplantlets

22. Yam Seedlings in Screenhouse (>Dec. 31, 2017)
0
20
40
60
80
100
120
140
160
180
200
TDr 89/02665
(83) TDr 89/02665
(6) TDa
98/01176
(13)
TDr 95/19177
(21)
Virus and Bacterial- Free Yam plantlets (Dec, 2017)
TDr 89/02665 (83)
TDr 89/02665 (6)
TDa 98/01176
(13)
TDr 95/19177 (21)
No.ofvirusandbacterialfreeSeedlings

23. Visit of Dr. M. Balogun/Ms. O. Odihi of IITA to NACGRAB
10th May. 2017

24. Visit of Dr. Marian Quain of Crop Research Institute (CRI),
Ghana to NACGRAB
30th August, 2017

25. Visit of Officials of NASC & YIIFSWA-II
Information Officer to NACGRAB
A
B
5th of October, 2017

26. Acclimatization of plantlets Potted Yam Seedlings in
NACGRAB Screen-house
Post-flask Management

27. Healthy Propagated Yam Varieties in NACGRAB Screen-
house

28. Harvested YIIFSWA-I yam tubers from NACGRAB field genebank.
(PONA, 02475, Amula, 18544, 02565, 02665)
Conservation of YIIFSWA-I Yam Varieties
in NACGRAB TC Field Genebank & Harvest

29. NACGRAB 2017 Achieved Milestone
S/N 2017 Activities Remarks
A Output 2.1.2: At least one fully functional scalable temporary
Immersion Bioreactor system TIBS established in Nigeria (at
NACGRAB, UI or NRCRI) and in Ghana at CRI by end of year
2018
Achieved
1 NACGRAB will supply NRCRI with TC and TIBs plantlets to plant
the aeroponics system (40 tables of 49 holes each). These virus
and disease free TC or TIBS plantlets should be produced from
the three yam varieties to be promoted in YIIFSWA-II.
Achieved
2 NACGRAB should be in the position to supply the needs of the
private seed company in initial plantlets to plant their
aeroponics system.
Achieved for 2017
3 Purchase all needed materials and chemicals to ensure the
effective running and functionally of TIBs at NACGRAB.
Achieved for 2017

30. Other NACGRAB 2017 Milestones
4 Contribute in the development of formal yam
seed system in Nigeria through supply of first
generation of plantlets.
Achieved for 2017
B Output 2.1.6: Context/Sahel has developed,
demonstrated, and trained researchers at NRCRI
and CRI on business model for pre-basic planting
material using TIBs. (Business components).
IITA/Context
C Output 2.1.7: Capacity for phytosanitary cleaning
and protection of in vitro plants (TC and TIBs),
aeroponics, vines and tubers established through
training and best-practices among NARIs and
private sector producing the pre-basic, basic seed
production in Nigeria and Ghana.
By IITA

31. ONGOING ACTION/S
• Acclimatization / Weaning
• Continuation of in vitro
Regeneration and
Multiplication of Clean
Yam Varieties
• Plan towards continuous
delivery of certified
clean yam seedlings.

32. Limitations
Contamination of Cultures
(TIBs & CTC)
Contamination of cultures is
being experienced continuously
especially in TIBs.
Post-flask Loss of Yam
Seedlings due to
Unfavorable Ecological
Conditions.
Seedlings were lost to abrupt weather change during
acclimatization & weaning.

33. Limitations
Ongoing Industrial Action
Man-power mobilization
becomes challenging as the
staff need to strike a balance
between union demands &
survival of the project by
working at odd hours.

34. Recommendations
Scaling up a SETTIS Bioreactor
facility for sustaining Yam
production in NACGRAB
 This will enhance
productivity
Scaling up of Alternative
Power Generating Equipment
 Procurement of Solar
panels to compliment
inverter & serve as
source of electric power
to charge batteries.

35. CONCLUSION
At NACGRAB,
irrespective of the
challenges,
we are intensifying
our efforts in
achieving
the delivery of the
objectives of
YIIFSWA-II i.e.
production of large
quantity of clean
yam seedlings.

36. NACGRAB TEAM - YIIFSWA
 Dr. Aladele S. E. (Dir/CEO)
• Mrs. Jamalledine Z. O.
• Dr. Afolayan A. O.
• Mr. Lawyer E. F.
• Mrs. Ajongbolo B. F.
• Mrs Olayode M. N.
• Mrs. Omosola O. T.
• Mr. Adedeji A.

37. ACKNOWLEDGEMENT
 Bill & Melinda Gate
Foundation - BMGF
 International Institute for
Tropical Agriculture – IITA
 All YIIFSWA Team
members & stakeholders

38. THANK
YOU