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Mr Govardhan Joshi
Introduction
 Skin and other tissue are richly supplied
with capillaries so when few ml of blood is
required capillary puncture is done
 Skin puncture is the method of choice in
pediatric patients
 Skin puncture can be used in adults with:
- extreme obesity
- sever burn
- Thrombotic tendencies
Site of obtaining blood-
 Finger tip
 Heel
 Great toe
 Ear lobe
Requirement-
 Lancet
 Spirit swab
 Sterile cotton
 Dry cotton
Technique:
 Identification of patient
 Select an appropriate puncture site
 Clean the puncture site with antiseptic
and allow the area to dry.
 Make the puncture with a sterile lancet
 Discard the first drop of blood by wiping
it away with a sterile pad
 Collect the specimen in a suitable
container
 Place gauze on the site and ask the
patient to apply pressure until bleeding
stops.
 Dispose of used supplies in biohazard
container.
 Thank the patient.
 Remove gloves and wash hands.
 Complete paperwork.
 Deliver sample to the laboratory
Disadvantages
 Can be used for only few tests
 Dilution of blood with tissue fluid will give
false low count

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Skin puncture method

  • 2. Introduction  Skin and other tissue are richly supplied with capillaries so when few ml of blood is required capillary puncture is done  Skin puncture is the method of choice in pediatric patients  Skin puncture can be used in adults with: - extreme obesity - sever burn - Thrombotic tendencies
  • 3. Site of obtaining blood-  Finger tip  Heel  Great toe  Ear lobe Requirement-  Lancet  Spirit swab  Sterile cotton  Dry cotton
  • 4. Technique:  Identification of patient  Select an appropriate puncture site  Clean the puncture site with antiseptic and allow the area to dry.  Make the puncture with a sterile lancet  Discard the first drop of blood by wiping it away with a sterile pad  Collect the specimen in a suitable container
  • 5.  Place gauze on the site and ask the patient to apply pressure until bleeding stops.  Dispose of used supplies in biohazard container.  Thank the patient.  Remove gloves and wash hands.  Complete paperwork.  Deliver sample to the laboratory
  • 6. Disadvantages  Can be used for only few tests  Dilution of blood with tissue fluid will give false low count