The use of carotenoids in poultry feeds is an environmentally friendly and profitable practice. therefore, it's sustainable.

1. Sustainable Animal Production
with CAROPHYLL®
People, Planet and Profit
April 2014

2. CAROPHYLL®
• CAROPHYLL® is the highest quality, best recognized brand of carotenoids in the
animal nutrition market
• Carotenoids are natural occurring substances that are added to the diet of
poultry to enhance the quality of the products (yolk and skin color, antioxidant
properties, vitelline membrane strength), and boost their reproductive
performance and immune capacity

3. Carotenoids in Nature
Carotenoids are very common molecules in nature.
They are synthesised by:
Bacteria Algae Fungi Plants
Nature produces 100 Million MT of Carotenoids per year
3 tons per second!

4. Carotenoids in Wild Birds
• Carotenoids are present in the animal kingdom (yellow, orange and red), and
are typically associated with colorful birds such as seagulls, canaries, cardinals
and flamingoes
• The color of the males is associated with good health and therefore with their
reproductive capacity
• In order to store carotenoids, birds need to be healthy enough to eat, digest
and absorb nutrients, so a pigmented bird is a healthy animal

5. Where CAROPHYLL® fits into the
Sustainability Equation?
CAROPHYLL® and People
Enhances the quality of poultry products via the use of safe nature
identical carotenoids in order to make them more nutritious and
appetizing
CAROPHYLL® and Planet
Production of nature-identical carotenoids with a lower carbon
footprint than chemically extracted carotenoids and with the
potential to reduce the carbon foot print of the poultry via better
feed efficiency
CAROPHYLL® and Profit
CAROPHYLL® boasts high Return on Investment when used as part of the
egg quality enhancement package and the breeder flock

6. CAROPHYLL® and People
• Increasing the attractiveness of healthy foods like eggs and chicken using
nature-identical carotenoids
• Increasing the antioxidant capacity of the egg and the strength of the yolk
(superior egg quality)
• Assessing the impact of carotenoids on the health of poultry and final consumer

7. CAROPHYLL® and People
• Increasing the attractiveness of healthy foods like eggs and chicken using
nature-identical carotenoids
In many countries,
people look for a
golden yolk as a
sign of a nutritive
and healthy egg
In China Brazil, Australia, Russia, India, Germany, Spain, Italy, France and others,
people prefer egg yolks with a YolkFan™ of 12 or more.

8. CAROPHYLL® and People
• Increasing the attractiveness of healthy foods like eggs and chicken using
“The yolk, for me, is really very
“The yolk must be
well-rounded and shiny.”
nature-identical carotenoids
“The yolk, for me, is really very important.
It must be yellow-orange.
Quite simply, because it will be much
more appetizing, it will draw the eye,
it will make the customer want to try it.”
“The appearance (of the egg) is very
important because it is what makes
you want to cook it, prepare it.”
important.
It must be yellow-orange.
Quite simply, because it will be much
more appetizing, it will draw the eye,
it will make the customer want to try it.”
“The appearance (of the egg) is very
important because it is what makes
you want to cook it, prepare it.”
“The yolk must be
well-rounded and shiny.”

9. CAROPHYLL® and People
• Increasing the attractiveness of healthy foods like eggs
and chicken using nature-identical carotenoids
The content of carotenoids in the raw yolk will certainly influence the outcome
of a simple boiled egg, which one would you rather have?

10. CAROPHYLL® and People
• Increasing the attractiveness of healthy foods like eggs and chicken using
Egg bread after supper for seventy
Brazilian guests in Bastos, Br. Self-service.
Breads were baked similarly using
eggs with different yolk shades.
At the end guests choose 3:1 the
golden bread.
nature-identical carotenoids

11. CAROPHYLL® and People
• Increasing the attractiveness of healthy foods like eggs and chicken using
nature-identical carotenoids
Scrambled eggs after breakfast for 100 international guests at the Hilton Hotel
in Zurich in 2013. Nobody touched the paler eggs.

12. Many egg products rely
on eggs as the sole
source of color.
The egg yolk content and
saturation are very
important
CAROPHYLL® and People
• Increasing the attractiveness of healthy foods like eggs and chicken using
nature-identical carotenoids

13. CAROPHYLL® and People
• Increasing the antioxidant capacity of the egg and the strength of the yolk
18
16
14
12
10
8
6
4
2
0
Effect of Canthaxanthin from CAROPHYLL® (6ppm) on the Total Antioxidant Status
a
Breeder hens egg yolks
Control
Canthaxanthin
(TAC, U/mL)of the breeder hens and eggs
(superior egg quality)
Zhang, at al., 2011. P. Sci. 90:1516
b
a
b

14. CAROPHYLL® and People
• Increasing the antioxidant capacity of the egg and the strength of the yolk
48
47
46
45
44
43
42
41
40
Vitelline membrane Rupture Force (mN)
95% CI for the Mean
P = 0.009
60ppm Caro Red Control
7.4
7.3
7.2
7.1
7
6.9
6.8
6.7
6.6
Vitelline membrane Rigidity (mN/mm)
95% CI for the Mean
P = 0.094
60ppm Caro Red Control
40 weeks hens- 7 days stored eggs
DSM non published

15. CAROPHYLL® and People
• Increasing the antioxidant capacity of the egg and the strength of the yolk
• Is helpful to separate the yolk and white
more easily
• Can be considered a measure for the egg
freshness
• Limits the watering of yolk and water loss
of white
(superior egg quality)
Hens fed 60ppm of
CAROPHYLL® Red
Regular eggs

16. CAROPHYLL® and People
• Assessing the impact of carotenoids on the health of poultry and final consumer
Canthaxanthin (active ingredient of CAROPHYLL® red) evaluations:
Safety is confirmed
• Authorized as a feed additive (for poultry and salmonids) and listed as a food additive
• This is the most- evaluated carotenoid in the world
- JECFA (toxicology) 1995
- SCF (toxicology) 1997
- SCAN (feed) 2002
- EFSA (feed/food) 2007, 2010, 2013
- …
• EFSA Oct 2010 (food) 2013 (feed)
- Safety of Canthaxanthin has been fully evaluated and re-confirmed
(all aspects of safety were reviewed)
CXN safety is confirmed by the evaluators

17. CAROPHYLL® and People
• Assessing the impact of carotenoids on the health of poultry and final consumer
ADI = 0.03mg CXN/kg BW/day.
What does this mean in practice?
• Max. approved level for CXN in eggs (Japan/EU) = 8mg CXN/kg feed
• 8mg CXN per kg feed = ~26mg CXN/kg egg yolk (worst case)
= 7.02mg CXN/kg egg (Egg yolk = 27% of the egg weight)
= 0.42mg CXN per egg (avg. egg weight (no shell) = 60gm)
• ADI for CXN = 0.03mg CXN/kg bw/day
= A 60kg human can safely consume up to 1.8mg of CXN per
day over a lifetime
Worst case scenario, a 60kg human can safely consume up to 4.3 eggs
containing CXN per day for a lifetime (1.8/0.42)

18. CAROPHYLL® and Planet
• Nature-identical carotenoids like the ones in CAROPHYLL®, have a much lower
carbon footprint than chemically extracted pigments
• CAROPHYLL® Red ( as part of the MaxiChick® breeder solution) has the potential
to reduce the amount of feed necessary to produce chicks
• CAROPHYLL® production complies with the very strict European rules to
minimize environmental impact

19. CAROPHYLL® and Planet
• Nature-identical carotenoids like the ones in CAROPHYLL®, have a much lower
carbon footprint than chemically extracted pigments
Life Cycle Assessment
Level of impact on environmental aspects based on estimation of graphic representations from Saling et al.200424
Aspect Nature-identical Natural extract
Per 100.000 eggs DYCF 12 Red Yellow Red Yellow
Energy Consumption 230 MJ 190 MJ 650 MJ 560 MJ
Emissions 12kg CO2 eq 12kg CO2 eq 52kg CO2 eq 130kg CO2 eq
Acidification potential 30kg SO2 eq 30kg SO2 eq 700kg SO2 eq 1300kg SO2 eq
Ozone depletion potential 0.002 mg CFC eq 0.005 mg CFC eq 0 mg CFC eq 0 mg CFC eq
Land use 0 m2 0 m2 37 m2 37 m2
Critical water volume 2000 m3 35000 m3 2000 m3 42000 m3
Risk potential* 6 units 7 units 36 units 16 units
Toxicity potential** 1.5 units 1 unit 6 units 6 units
* Risk potential calculated different risks depending on reaction temperatures, pressures, inflammable materials, etc. as well as accident statistics from different industry
sectors in different countries. Transportation risks were estimated where longer transportation distances resulted in higher risk evaluations.
** Toxicity potential was determined using an assessment method based on the R-phrases of the Hazardous Substances Regulation Act (GefStoffV) and based on units
calculated in a matrix with weighted points based on severity according to their toxicity potential.

20. CAROPHYLL® and Planet
• Nature-identical carotenoids like the ones in CAROPHYLL®, have a much lower
carbon footprint than chemically extracted pigments
50000
40000
30000
20000
10000
0
Yellow and red,
chemical
Yellow and red,
plant-extract
g Emittents/CB
Carbon dioxide
140
120
100
80
60
40
20
0
Yellow and red,
chemical
Yellow and red,
plant-extract
g Emittents/CB
Nitrous oxide
Chlorinated and fluorinated carbon
Methane
Saling, et al., 2006. Journal of Sustainable Agriculture, Vol. 29(1)

21. CAROPHYLL® and Planet
• CAROPHYLL® Red (as part of the MaxiChick® solution for breeders) has the
potential to reduce the amount of feed necessary to produce one day old chicks,
reducing the environmental impact of Poultry
3740
3720
3700
3680
3660
3640
3620
3600
3580
3560
Chicks/Ton feed*
Control MaxiChick
*MaxiChick® App for Ross 308 improving one settable egg, 1% fertility and 1% hatchability.

22. CAROPHYLL® and Planet
• CAROPHYLL® production complies with the very strict European rules to
minimize environmental impact
Village Neuf is situated in Alsace, France, along the banks of the Rhine River

23. 900
800
700
600
500
400
300
200
100
0
Numbers of Salmon (n)
Year
Delta du Rhin
Rhin inferieur
Rhin moyen
Rhin superieur
Programme Rhine 2020: Salmon 2020
Up until 2009:
5,000 salmon have returned to the Rhine
2020: A population of 7,000 – 21,000 salmon will migrate into the Rhine each year
Adapted from:http://www.unece.org/fileadmin/DAM/env/water/cadialogue/docs/Almaty_Oct2010/Eng/Hofstra_Eng.pdf
The Rhine river is an ecological success story, and DSM takes responsible
care of the river, thanks to its strict environmental policies. We are proud
to be a good neighbor.

24. CAROPHYLL® and Profit
• The return on investment of using CAROPHYLL® is around 100:1 when used for
egg improvement
• CAROPHYLL® Red ( as part of the MaxiChick® solution for breeders) maximizes
the profit of the breeder farmer
• CAROPHYLL® is more cost effective than chemically extracted carotenoids

25. CAROPHYLL® and Profit
• The return on investment of using CAROPHYLL® is around 100:1
when used for egg improvement
80
70
60
50
40
30
20
10
Price and DYCF in Beijing
y=5.1823x – 21.087
R2 = 0.3809
In Beijing a direct relationship between price and
egg yolk color was found. Eggs over 30 RMB/kg
were always over 9 on the DYCF.
0
4 5 6 7 8 9 10 11 12 13 14 15
Price/RMbkg
DSM YCF
60
50
40
30
20
10
0
Price and DYCF in Shangai
4 5 6 7 8 9 10 11 12 13 14 15
Price/RMbkg
DSM YCF
In Shangai, two distinctive groups were found.
Very pale eggs (DYCF<6) comprise 31% of the
sample. Golden eggs (DYCF >9) comprised 69% of
the supermarket offering. All the eggs with prices
over 40 RMBs were well pigmented.
Approx. Carotenoid investment to get to DYCF15 = 0.09 RMB/kg egg
y=1.7566x + 16.109
R2 = 0.3073

26. Eggs in Spain
Summary:
1. Avg. YolkFan™ 11.4 (10-14)
2. Price related to claim O >FR > C
3. Price related to YolkFan ™
4. ROI= 0.0404/.000102=396
1 USD=0.7248 €
0.5
0.4
0.3
0.2
0.1
Egg prices in Madrid and Alcala according to attributes
0.5
0.4
0.3
0.2
0.1
Egg prices in Madrid and Alcala according to YolkFan™,
€/egg (not-organics)
Investment to go from 10 to 14 in YolkFan™= 0.000102 €/YolkFan
unit/egg
C = Conventional
GF = GMO Free
B = Barn
FR = Free-range
O3 = Omega 3
VE = Vitamin E
O = Organic
W = Welfare check
M = Medium
L = large
0
C, M C, M C, M-L
C, L C, L C, XL FR FR FR FR FR O
€/egg
Attributes
y = 0.0404x - 0.2606
R² = 0.2178
0
9 9.5 10 10.5 11 11.5 12 12.5 13 13.5

27. Eggs in Russia
11
10
9
8
7
6
5
4
Eggs in Moscow according to YolkFan™, RUB/Egg
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Summary:
1. Avg YolkFan™ 11 (3-14)
2. Price related to claim O >FR > C
3. Price related to YolkFan™
4. ROI= 0.1609/.00414=38.8
Investment to go from 8 to 14 in YolkFan™= 0.00414 RUB/YolkFan
unit/egg
1 USD=36.15 RUB
y = 0.1609x + 5.3997
R² = 0.22177

28. Egg Price vs Yolkfan™, Japan
(non-organic)
120
100
80
60
40
20
0
Ordinary MCoop brand Ordinary L Freshness Enriched
nutrient
Enriched
Folic acid
Iodine egg Rice
Average Price by claim, JPY/egg
160
140
120
100
80
60
40
20
0
Egg prices as affected by YolkFan™, JPY/egg
8 9 10 11 12 13 14 15
YolkFan™
Summary:
1. Avg YolkFan™ 11.6 (8-15)
2. Price related to claim
3. Price related to YolkFan™
4. ROI= 7.2679/.011=660.72
1 USD=102.157 JPY
y = 7.2679x - 57.574
R² = 0.31898
Investment to go from 8 to 15 in YolkFan™= 0.011 JPY/YolkFan
unit/egg

29. Egg Price in Ukraine
2.1
1.9
1.7
1.5
1.3
1.1
0.9
0.7
0.5
Egg pricing as affected by YolkFan™ , UAH/egg
5 6 7 8 9 10 11 12 13 14
UAH/egg
YolkFan™
Summary:
1. Avg YolkFan™ 9.3 (6-13)
2. Price related to claim
3. Price related to YolkFan™
4. ROI= 0.0709/.00091=77.9
Investment to go from 6 to 14 in YolkFan™= 0.00091 UAH/YolkFan
unit/egg
1 USD=9.7 UAH
y = 0.0709x + 0.6192
R² = 0.29392

30. Egg Price in Brazil
15
14
13
12
11
10
1.20
1.00
0.80
0.60
0.40
0.20
0.00
Price per egg (NON-ORGANIC) as affected by YolkFan™, BRL/eggPREÇO
3 4 5 6 7 8 9 10 11 12 13 14 15
BRL/egg
YolkFan™
Summary:
1. Avg YolkFan™ 9 (3-15)
2. YolkFan value related to claim
3. Price related to YolkFan™ and claim
4. ROI= 0.0294/.000024=123
1 USD=102.157 JPY
y = 0.0294x + 0.232 R² = 0.23427
Investment to go from 6 to 14 in YolkFan™= 0.00024 BRL/YolkFan
unit/egg
3 4 5 6 7 8 9
CA
CA
CA
CA
CA
CA
O3
O3VE
O3VE
O3VE
OG
OG
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
RE
VE
YolkFan
Attributes and YolkFan™ in the Brazilian egg shell market
VE = Vitamin
Enriched
RE = Regular
OG = Organic
O3VE = Omega 3
+ Vitamin E
O3 = Omega 3
CA = Caipira

31. CAROPHYLL® and Profit
• CAROPHYLL® Red (as part of the MaxiChick® solution for breeders) maximize
• Using the MaxiChick calculator with
the following assumptions:
- Ross 308 performing at standard
- Improvement of +1 settable eggs
- Improvement of +1% fertility
- Improvement of +1% hatchability
• That means 4.1 and 100 chicks more
per housed hen and per MT of feed
respectively
• At 0.30 € per chick and 6€/Mt
inclusion rate, that means an ROI
of 5 to 1
the profit of the breeder farmer

32. CAROPHYLL® and Profit
• CAROPHYLL® is more cost effective than chemically extracted carotenoids
Equivalent of 1 kg of pure Canthaxanthin
from CAROPHYLL®, to different sources
of red carotenoids for Egg pigmentation
600
700
500
1
10
2.5
0 500 1000
Canthaxanthin
CAROPHYLL® Red, 10%
Pure capsanthin/capsorubin
Stable Paprika extract, 5 g/kg
High quality paprika
Low quality paprika
Equivalent of 1 kg of pure Apo-ester from
CAROPHYLL®, to different sources of
yellow carotenoids for Egg pigmentation
180
210
150
1
10
3
0 50 100 150 200 250
Apo-ester
CAROPHYLL® yellow, 10%
Lut/Zea pure
tagetes, 2% stable
Tagetes 2% High quality
Tagetes 2% low quality
The high replacement rates and the superior performance of the CAROPHYLL®
carotenoids, make them the most economical solution to improve the quality of eggs

33. Where CAROPHYLL® Fits into the
Sustainability Equation?
CAROPHYLL® and People
Enhance the quality of poultry products via the use of safe nature
identical carotenoids in order to make them more nutritious and
appetizing
CAROPHYLL® and Planet
Production of nature-identical carotenoids with a lower carbon
footprint than chemically extracted carotenoids and with the
potential to reduce the carbon foot print of the poultry via better
feed efficiency
CAROPHYLL® and Profit
CAROPHYLL® boast high Return on Investment when used as part of
the egg quality enhancement package and the breeder flock
Triple check: Sustainability is the business driver for
CAROPHYLL® as well!!
✓
✓
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