Enzyme-linked immunosorbent assay (ELISA) is an immunoassay used to measure antibodies, antigens, proteins, & glycoproteins in biological samples. ELISA is a plate-based technique.

1. Applications of ELISA
(Enzyme-Linked
Immunosorbent Assay)

2. synopsis
Introduction
Definition
Applications

3. introduction
• it is an immunological assay
Note
Immunological assays / Immunoassay (IA): biochemical test

4. definition
• it is a plate based technique
• used to detect & quantify peptides, antibodies, proteins &
hormones

5. applications

6. Detect & Measure the Presence of Antibodies in
the blood
 Autoantibodies (ANTI-DSDNA, ANTI-DSG1, ANA, etc.)
 Antibodies against infectious disease
(ANTIBACTERIAL, ANTIVIRAL, ANTIFUNGAL)
 Hepatitis A, B, C, HIV, etc

7. Detect & Estimate the Levels of Tumor
Markers
 PROSTATE-SPECIFIC ANTIGEN (PSA)
 CARCINOEMBRYONIC ANTIGEN (CEA)

8. Detect & Estimate Hormone Levels
 luteinizing hormone
 follicular stimulating hormone
 prolactin
 testosterone
 human chorionic gonadotropin (hcg)
Hormone: chemical substances (act like messenger molecules)
Note

9. tracking disease outbreaks
 cholera
 HIV
• influenza

10. detecting past exposures
 HIV
 lyme disease
 hepatitis

11. screening donated blood for possible viral
contaminants
 ANTI-HIV-1/2
 ANTI-HCV
 HBSAG

12. detecting drug abuse
 amphetamine
 methamphetamine
 3,4-methylenedioxymethamphetamine
 cocaine
 benzoylecgonine

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YOU
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